CELL SPHERE PREPARATION DEVICE AND PREPARATION METHOD - TSINGHUA SHENZHEN INT GRADUATE SCHOOL

Title:

CELL SPHERE PREPARATION DEVICE AND PREPARATION METHOD

Document Type and Number:

WIPO Patent Application WO/2024/017118

Kind Code:

A1

Abstract:

A cell sphere preparation device and preparation method. The cell sphere preparation method comprises: an aqueous phase liquid reservoir (202), a liquid storage tube (204) and a first pump (207) being in communication in sequence, and the first pump (207) pumping cell suspension into the liquid storage tube (204); a second pump (205), the liquid storage tube (204) and a first inlet (301) of a microfluidic chip (208) being in communication in sequence, and the second pump (205) delivering the cell suspension in the liquid storage tube (204) to the first inlet (301); making an oil phase material flow to a second inlet (302) of the microfluidic chip, such that droplets of the cell suspension are formed in the microfluidic chip (208); and solidifying the droplets to form cell spheres. The cell sphere preparation device and preparation method provided can improve the sample injection efficiency of a microfluidic chip, and thus improve the preparation efficiency of cell spheres; moreover, the cell suspension injection amount of the microfluidic chip can be accurately controlled, thereby ensuring the quality of the cell spheres and improving the quality stability of the cell spheres.

Inventors:

MA SHAOHUA (CN)
YANG HAOWEI (CN)

Application Number:

PCT/CN2023/106991

Publication Date:

January 25, 2024

Filing Date:

July 12, 2023

Export Citation:

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Assignee:

TSINGHUA SHENZHEN INT GRADUATE SCHOOL (CN)

International Classes:

C12M3/00; B01L3/00; C12M1/00; C12M1/34; C12M1/36; C12M1/38

Foreign References:

CN115287185A	2022-11-04
CN107418872A	2017-12-01
JP3109378U	2005-05-19
CN113583960A	2021-11-02
JPH0331766A	1991-02-12
US6558946B1	2003-05-06
CN110711608A	2020-01-21
JP2021067641A	2021-04-30
US20190105279A1	2019-04-11

Other References:

ZHANG,T. ET AL.: "One-Step Generation and Purification of Cell-Encapsulated Hydrogel Microsphere With an Easily Assembled Microfluidic Device.", FRONT. BIOENG. BIOTECHNOL., vol. 9, 28 January 2022 (2022-01-28), XP055930020, DOI: 10.3389/fbioe.2021.816089

Attorney, Agent or Firm:

JIAQUAN IP LAW (CN)

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