Title:
CELL WITH TARGETED INTEGRATION, METHOD FOR PREPARING SAME, AND METHOD FOR PRODUCING TARGET GENE EXPRESSION PRODUCT
Document Type and Number:
WIPO Patent Application WO/2023/246626
Kind Code:
A1
Abstract:
Provided are a cell with targeted integration, a method for preparing same, and a method for producing a target gene expression product. The cell with targeted integration comprises a nucleic acid fragment set forth in SEQ ID NO. 1 comprising an integrated exogenous nucleotide sequence. By means of massive screenings in the early stage, the nucleic acid fragment set forth in SEQ ID NO. 1 was found. By integrating the exogenous nucleotide sequence into the nucleic acid fragment, the obtained cell with targeted integration features stability and a high yield.
Inventors:
CHEN LIANG (CN)
DENG XINYU (CN)
YANG XIAOLI (CN)
LIANG GUOLONG (CN)
DENG XINYU (CN)
YANG XIAOLI (CN)
LIANG GUOLONG (CN)
Application Number:
PCT/CN2023/100580
Publication Date:
December 28, 2023
Filing Date:
June 16, 2023
Export Citation:
Assignee:
SHENZHEN TAILI BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N15/11; C12N5/10; C12N15/85; C12N15/90
Foreign References:
| CN107109434A | 2017-08-29 | |||
| CN110997925A | 2020-04-10 | |||
| US20220119985A1 | 2022-04-21 |
Other References:
DATABASE Nucleotide 12 July 2020 (2020-07-12), HILLIARD, W. ET AL.: "Cricetulus griseus strain 17A/GY chromosome 3, alternate assembly CriGri-PICRH-1.0, whole genome shotgun sequence", XP093120994, retrieved from NCBI Database accession no. NC_048596
JIAN-FEN LIN, LUO SHUN: "Research Progress on Stable Hot Spot Sites in the Genome of CHO Cells", CHINA BIOTECHNOLOGY, vol. 42, no. 3, 25 March 2022 (2022-03-25), pages 72 - 81, XP093121002
JAE SEONG LEE, KALLEHAUGE THOMAS BEUCHERT, PEDERSEN LASSE EBDRUP, KILDEGAARD HELENE FAUSTRUP: "Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway", SCIENTIFIC REPORTS, vol. 5, no. 1, 1 July 2015 (2015-07-01), pages 1 - 11, XP055373118, DOI: 10.1038/srep08572
JIAN-FEN LIN, LUO SHUN: "Research Progress on Stable Hot Spot Sites in the Genome of CHO Cells", CHINA BIOTECHNOLOGY, vol. 42, no. 3, 25 March 2022 (2022-03-25), pages 72 - 81, XP093121002
JAE SEONG LEE, KALLEHAUGE THOMAS BEUCHERT, PEDERSEN LASSE EBDRUP, KILDEGAARD HELENE FAUSTRUP: "Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway", SCIENTIFIC REPORTS, vol. 5, no. 1, 1 July 2015 (2015-07-01), pages 1 - 11, XP055373118, DOI: 10.1038/srep08572
Attorney, Agent or Firm:
CHINA ZHENGHE INTELLECTUAL PROPERTY AGENCY (CN)
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