Title:
CELLS HAVING HIGH ADAPTABILITY UNDER HYPOXIC CONDITIONS, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/145700
Kind Code:
A1
Abstract:
The present application relates to cells having high adaptability under hypoxic conditions, and a preparation method therefor. Particularly, the present specification provides cells having high adaptability under hypoxic conditions, comprising at least one engineered gene having indels within a wild-type gene selected from the group consisting of HIF1AN, HIF3A, PHD2, TLR4 and PAI1. In addition, the present specification provides, as a method for preparing cells having high adaptability under hypoxic conditions, a gene editing method comprising introducing a CRISPR/Cas9 system into cells.
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Inventors:
SHIN EUN JI (KR)
LEE KANG IN (KR)
LEE JAE YOUNG (KR)
LEE KANG IN (KR)
LEE JAE YOUNG (KR)
Application Number:
PCT/KR2021/000546
Publication Date:
July 22, 2021
Filing Date:
January 14, 2021
Export Citation:
Assignee:
TOOLGEN INC (KR)
International Classes:
C12N5/0775; C07K14/47; C12N9/22; C12N15/10; C12N15/11
Domestic Patent References:
| WO2018231018A2 | 2018-12-20 |
Foreign References:
| KR20150101446A | 2015-09-03 |
Other References:
FILHO DANIEL MENDES; CARVALHO RIBEIRO PATRÍCIA DE; OLIVEIRA LUCAS FELIPE; SANTOS ANA LUIZA DOS; PARREIRA RICARDO CAMBRAIA; PINTO M: "Enhancing the Therapeutic Potential of Mesenchymal Stem Cells with the CRISPR-Cas System", STEM CELL REVIEWS AND REPORTS, HUMANA PRESS INC., US, vol. 15, no. 4, 30 May 2019 (2019-05-30), US, pages 463 - 473, XP036834616, ISSN: 1550-8943, DOI: 10.1007/s12015-019-09897-0
MAHON P C, HIROTA K, SEMENZA G L: "FIH-1: A novel protein that interacts with HIF-1alpha and VHL to mediate repression of HIF-1 transcriptional activity.", GENES & DEVELOPMENT, COLD SPRING HARBOR LABORATORY PRESS, PLAINVIEW, NY., US, vol. 15, no. 20, 15 October 2001 (2001-10-15), US, pages 2675 - 2686, XP002254663, ISSN: 0890-9369, DOI: 10.1101/gad.924501
YANG SHENG-LI, WU CHAO, XIONG ZHI-FAN, FANG XIEFAN: "Progress on hypoxia-inducible factor-3: Its structure, gene regulation and biological function (Review)", MOLECULAR MEDICINE REPORTS, vol. 12, no. 2, 2015, GR, pages 2411 - 2416, XP009529034, ISSN: 1791-2997, DOI: 10.3892/mmr.2015.3689
NATH BHARATH, SZABO GYONGYI: "Hypoxia and hypoxia inducible factors: Diverse roles in liver diseases", HEPATOLOGY, JOHN WILEY & SONS, INC., US, vol. 55, no. 2, 1 February 2012 (2012-02-01), US, pages 622 - 633, XP055828822, ISSN: 0270-9139, DOI: 10.1002/hep.25497
LEE SEAHYOUNG, CHOI EUNHYUN, CHA MIN-JI, HWANG KI-CHUL: "Cell Adhesion and Long-Term Survival of Transplanted Mesenchymal Stem Cells: A Prerequisite for Cell Therapy", OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, HINDAWI PUBLISHING CORPORATION, US, vol. 2015, 1 January 2015 (2015-01-01), US, pages 1 - 9, XP055828821, ISSN: 1942-0900, DOI: 10.1155/2015/632902
MAHON PCHIROTA KSEMENZA GL: "FIH-1: a novel protein that interacts with HIF-lalpha and VHL to mediate repression of HIF-1 transcriptional activity", GENES DEV, vol. 15, no. 20, 2001, pages 2675 - 2686, XP002254663, DOI: 10.1101/gad.924501
See also references of EP 4092111A4
MAHON P C, HIROTA K, SEMENZA G L: "FIH-1: A novel protein that interacts with HIF-1alpha and VHL to mediate repression of HIF-1 transcriptional activity.", GENES & DEVELOPMENT, COLD SPRING HARBOR LABORATORY PRESS, PLAINVIEW, NY., US, vol. 15, no. 20, 15 October 2001 (2001-10-15), US, pages 2675 - 2686, XP002254663, ISSN: 0890-9369, DOI: 10.1101/gad.924501
YANG SHENG-LI, WU CHAO, XIONG ZHI-FAN, FANG XIEFAN: "Progress on hypoxia-inducible factor-3: Its structure, gene regulation and biological function (Review)", MOLECULAR MEDICINE REPORTS, vol. 12, no. 2, 2015, GR, pages 2411 - 2416, XP009529034, ISSN: 1791-2997, DOI: 10.3892/mmr.2015.3689
NATH BHARATH, SZABO GYONGYI: "Hypoxia and hypoxia inducible factors: Diverse roles in liver diseases", HEPATOLOGY, JOHN WILEY & SONS, INC., US, vol. 55, no. 2, 1 February 2012 (2012-02-01), US, pages 622 - 633, XP055828822, ISSN: 0270-9139, DOI: 10.1002/hep.25497
LEE SEAHYOUNG, CHOI EUNHYUN, CHA MIN-JI, HWANG KI-CHUL: "Cell Adhesion and Long-Term Survival of Transplanted Mesenchymal Stem Cells: A Prerequisite for Cell Therapy", OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, HINDAWI PUBLISHING CORPORATION, US, vol. 2015, 1 January 2015 (2015-01-01), US, pages 1 - 9, XP055828821, ISSN: 1942-0900, DOI: 10.1155/2015/632902
MAHON PCHIROTA KSEMENZA GL: "FIH-1: a novel protein that interacts with HIF-lalpha and VHL to mediate repression of HIF-1 transcriptional activity", GENES DEV, vol. 15, no. 20, 2001, pages 2675 - 2686, XP002254663, DOI: 10.1101/gad.924501
See also references of EP 4092111A4
Attorney, Agent or Firm:
IPS PATENT FIRM (KR)
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