CELLS HAVING A SPECTRAL SIGNATURE, AND METHODS OF PREPARATION AND USE THEREOF

Title:

CELLS HAVING A SPECTRAL SIGNATURE, AND METHODS OF PREPARATION AND USE THEREOF

Document Type and Number:

WIPO Patent Application WO2002029410

Kind Code:

A3

Abstract:

Methods, compositions and articles of manufacture for encoding a cell with semiconductor nanocrystals and/or other fluorophors are provided. The encoded cells can be subjected to functional assays in mixed populations, and an assay result can be determined and associated with individual cells by virtue of their code. The methods are particularly useful in multiplex settings where a plurality of encoded cells are to be assayed. The methods are used in screening methods for G protein coupled receptors (GPCRs), for identifying the ligands and functions of orphan GPCRs, and for screening for modulators of GPCRs. Kits comprising reagents for performing such methods are also provided.

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Inventors:

BRUCHEZ MARCEL P (US)
DANIELS R HUGH (US)
DIAS JENNIFER (US)
MATTHEAKIS LARRY C (US)

Application Number:

PCT/US2001/031410

Publication Date:

January 30, 2003

Filing Date:

October 05, 2001

Export Citation:

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Assignee:

QUANTUM DOT CORP (US)
BRUCHEZ MARCEL P (US)
DANIELS R HUGH (US)
DIAS JENNIFER (US)
MATTHEAKIS LARRY C (US)

International Classes:

C12N5/00; C12N5/06; G01N33/50; G01N33/566; G01N33/58; (IPC1-7): G01N33/53; G01N33/58; G01N33/533

Foreign References:

EP0990903A1	2000-04-05
US5990479A	1999-11-23
US6051386A	2000-04-18

Other References:

BRUCHEZ M JR ET AL: "Semiconductor nanocrystals as fluorescent biological labels", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 281, 25 September 1998 (1998-09-25), pages 2013 - 2016, XP002125872, ISSN: 0036-8075
CHAN WCW ET AL: "Quantum Dot bioconjugates for ultrasensitive nonisotopic detection", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 281, 25 September 1998 (1998-09-25), pages 2016 - 2018, XP002125871, ISSN: 0036-8075
LACOSTE T D ET AL: "SUPER RESOLUTION MOLECULAR RULER USING SINGLE QUANTUM DOTS", BIOPHYSICAL JOURNAL, NEW YORK, US, US, vol. 78, January 2000 (2000-01-01), pages 402A, XP000933548, ISSN: 0006-3495

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