Title:
CELLS AND METHODS FOR PRODUCING ISOBUTYRIC ACID
Document Type and Number:
WIPO Patent Application WO/2012/109534
Kind Code:
A3
Abstract:
Disclosed herein are cells and methods for renewably producing isobutyrate. In some cases, the cells can include a heterologous DNA that encodes at least one enzyme that catalyzes the conversion of isobutyraldehyde to isobutyrate. In other cases, the cells can include a genetically modified enzyme that catalyzes the conversion of isobutyraldehyde to isobutyrate to a degree greater than the wild-type version of the enzyme. In other cases, the cells can include one or more enzyme that catalyze the conversion of 2-ketovaline to isobutyrate. Generally, methods include growing the cells in a medium that includes a carbon source that the cells are able to convert to isobutyrate.
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Inventors:
ZHANG KECHUN (US)
XIONG MINGYONG (US)
WOODRUFF ADAM P (US)
XIONG MINGYONG (US)
WOODRUFF ADAM P (US)
Application Number:
PCT/US2012/024640
Publication Date:
October 04, 2012
Filing Date:
February 10, 2012
Export Citation:
Assignee:
UNIV MINNESOTA (US)
ZHANG KECHUN (US)
XIONG MINGYONG (US)
WOODRUFF ADAM P (US)
ZHANG KECHUN (US)
XIONG MINGYONG (US)
WOODRUFF ADAM P (US)
International Classes:
C12N1/20; C12N9/00; C12N9/16; C12P7/52
Domestic Patent References:
| WO2010017230A2 | 2010-02-11 |
Foreign References:
| US4452999A | 1984-06-05 |
Other References:
FERRANDEZ A ET AL: "MOLECULAR CHARACTERIZATION OF PADA, A PHENYLACETALDEHYDE DEHYDROGENASE FROM ESCHERICHIA COLI", FEBS LETTERS, vol. 406, no. 1/02, 4 July 1997 (1997-07-04), pages 23 - 27, XP001026135, ISSN: 0014-5793
JAMES M CLOMBURG ET AL: "Biofuel production in Escherichia coli: the role of metabolic engineering and synthetic biology", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 86, no. 2, 9 February 2010 (2010-02-09), pages 419 - 434, XP019799970, ISSN: 1432-0614
PAMELA P. PERALTA-YAHYA ET AL: "Advanced biofuel production in microbes", BIOTECHNOLOGY JOURNAL, vol. 5, no. 2, 1 February 2010 (2010-02-01), pages 147 - 162, XP055027158, ISSN: 1860-6768
TIANGANG LIU ET AL: "Genetic Engineering of Escherichia coli for Biofuel Production", ANNUAL REVIEW OF GENETICS, vol. 44, no. 1, 1 December 2010 (2010-12-01), pages 53 - 69, XP055027159, ISSN: 0066-4197
KECHUN ZHANG ET AL: "A Synthetic Metabolic Pathway for Production of the Platform Chemical Isobutyric Acid", CHEMSUSCHEM, vol. 4, no. 8, 22 August 2011 (2011-08-22), pages 1068 - 1070, XP055027005, ISSN: 1864-5631
JAMES M CLOMBURG ET AL: "Biofuel production in Escherichia coli: the role of metabolic engineering and synthetic biology", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 86, no. 2, 9 February 2010 (2010-02-09), pages 419 - 434, XP019799970, ISSN: 1432-0614
PAMELA P. PERALTA-YAHYA ET AL: "Advanced biofuel production in microbes", BIOTECHNOLOGY JOURNAL, vol. 5, no. 2, 1 February 2010 (2010-02-01), pages 147 - 162, XP055027158, ISSN: 1860-6768
TIANGANG LIU ET AL: "Genetic Engineering of Escherichia coli for Biofuel Production", ANNUAL REVIEW OF GENETICS, vol. 44, no. 1, 1 December 2010 (2010-12-01), pages 53 - 69, XP055027159, ISSN: 0066-4197
KECHUN ZHANG ET AL: "A Synthetic Metabolic Pathway for Production of the Platform Chemical Isobutyric Acid", CHEMSUSCHEM, vol. 4, no. 8, 22 August 2011 (2011-08-22), pages 1068 - 1070, XP055027005, ISSN: 1864-5631
Attorney, Agent or Firm:
GRAM, Christophe D. (Raasch & Gebhardt P.A.,P.O. Box 58133, Minneapolis Minnesota, US)
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