Title:
CELLULOSE-SYNTHETIZING MICROBE TRANSFECTANT AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/149583
Kind Code:
A1
Abstract:
The present invention addresses the problem of realizing a cellulose-synthesizing microbe transfectant capable of producing glucose or cellobiose from a carbon source such as methanol. A transfectant according to one embodiment of the present invention resolves the abovementioned problem by introducing a cellulase gene and a cell-surface protein gene into a cellulose-synthesizing microbe.
Inventors:
ASO YUJI (JP)
Application Number:
PCT/JP2023/003994
Publication Date:
August 10, 2023
Filing Date:
February 07, 2023
Export Citation:
Assignee:
SUMITOMO CHEMICAL CO (JP)
NAT UNIV CORP KYOTO INST TECHNOLOGY (JP)
NAT UNIV CORP KYOTO INST TECHNOLOGY (JP)
International Classes:
C12N15/31; C12N1/21; C12N15/55; C12N15/62; C12N15/74; C12P19/14
Domestic Patent References:
| WO2014080070A1 | 2014-05-30 |
Foreign References:
| JPH09503649A | 1997-04-15 | |||
| US20150044327A1 | 2015-02-12 |
Other References:
FRANCISCO J A, ET AL.: "SPECIFIC ADHESION AND HYDROLYSIS OF CELLULOSE BY INTACT ESCHERICHIACOLI EXPRESSING SURFACE ANCHORED CELLULASE OR CELLULOSE BINDING DOMAINS", BIOTECHNOLOGY. THE INTERNATIONAL MONTHLY FOR INDUSTRIAL BIOLOGY, NATURE PUBLISHING GROUP, US, vol. 11, 1 April 1993 (1993-04-01), US , pages 491 - 496, XP002947290, ISSN: 0733-222X, DOI: 10.1038/nbt0493-491
GAO DONGFANG, LUAN YAQI, WANG QIAN, LIANG QUANFENG, QI QINGSHENG: "Construction of cellulose-utilizing Escherichia coli based on a secretable cellulase", MICROBIAL CELL FACTORIES, vol. 14, no. 1, 1 December 2015 (2015-12-01), pages 159, XP093083362, DOI: 10.1186/s12934-015-0349-7
NIGEL F. DELANEY, MARIA E. KACZMAREK, LEWIS M. WARD, PAIGE K. SWANSON, MING-CHUN LEE, CHRISTOPHER J. MARX: "Development of an Optimized Medium, Strain and High-Throughput Culturing Methods for Methylobacterium extorquens", PLOS ONE, vol. 8, no. 4, pages e62957, XP055260103, DOI: 10.1371/journal.pone.0062957
GAO DONGFANG, LUAN YAQI, WANG QIAN, LIANG QUANFENG, QI QINGSHENG: "Construction of cellulose-utilizing Escherichia coli based on a secretable cellulase", MICROBIAL CELL FACTORIES, vol. 14, no. 1, 1 December 2015 (2015-12-01), pages 159, XP093083362, DOI: 10.1186/s12934-015-0349-7
NIGEL F. DELANEY, MARIA E. KACZMAREK, LEWIS M. WARD, PAIGE K. SWANSON, MING-CHUN LEE, CHRISTOPHER J. MARX: "Development of an Optimized Medium, Strain and High-Throughput Culturing Methods for Methylobacterium extorquens", PLOS ONE, vol. 8, no. 4, pages e62957, XP055260103, DOI: 10.1371/journal.pone.0062957
Attorney, Agent or Firm:
HARAKENZO WORLD PATENT & TRADEMARK (JP)
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