Title:
CEPHALOSPORIN HYDROXYLASE MUTANT, ENCODING DNA OF MUTANT, METHOD FOR USING MUTANT AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/001034
Kind Code:
A1
Abstract:
A cephalosporin hydroxylase mutant, encoding DNA of the mutant, a method for using the mutant and an application thereof. An amino acid sequence of the cephalosporin hydroxylase mutant uses SEQ ID NO:2 as a reference sequence in a sequence listing, and has a mutation located in at least one of position 29 glycine, position 40 alanine, position 41 glycine, position 182 arginine and position 272 threonine; compared to wild-type cephalosporin hydroxylase, the cephalosporin hydroxylase mutant has an enzymatic activity at least 10% higher and a heat resistance at least 150% higher.
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Inventors:
LUI YUK SUN (CN)
CHENG SHIU MING (CN)
CHOW YUEN WING (CN)
SIU YAU LUNG (CN)
WANG JUN (CN)
CHENG SHIU MING (CN)
CHOW YUEN WING (CN)
SIU YAU LUNG (CN)
WANG JUN (CN)
Application Number:
PCT/CN2017/086843
Publication Date:
January 04, 2018
Filing Date:
June 01, 2017
Export Citation:
Assignee:
BIORIGHT WORDWIDE CO LTD (GB)
LUI YUK SUN (CN)
LUI YUK SUN (CN)
International Classes:
C12N15/53; C12N9/02; C12P35/02
Domestic Patent References:
| WO2008107782A2 | 2008-09-12 |
Foreign References:
| CN104364376A | 2015-02-18 |
Other References:
LLOYD, M. D. ET AL.: "Controlling the Substrate Selectivity of Deacetoxycephalosporin/deacetylcephalosporin C Synthase", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 279, no. 15, 9 April 2004 (2004-04-09), pages 15420 - 15426, XP002305107
WU, X. B. ET AL.: "C-terminus Mutations of Acremonium Chrysogenum Deacetoxy/deacetyl Cephalosporin C Synthase with Improved Activity toward Penicillin Analogs", FEMS MICROBIOLOGY LETTERS., vol. 246, no. 1, 8 April 2005 (2005-04-08), pages 103 - 110, XP027871731
HAN, SHU ET AL.: "Research Progress on Biosynthesis and Regulatory Mechanisms of Cephamycin C in Streptomyces Clavuligerus", CHINESE JOURNAL OF ANTIBIOTICS, vol. 40, no. 8, 31 August 2015 (2015-08-31), pages 561 - 569, XP055586704
WEI, C. L. ET AL.: "Directed Evolution of Streptomyces Clavuligerus Deacetoxycephalosporin C Synthase for Enhancement of Penicillin G Expansion", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 71, no. 12, 31 December 2005 (2005-12-31), pages 8873 - 8880, XP055450212
See also references of EP 3480313A4
WU, X. B. ET AL.: "C-terminus Mutations of Acremonium Chrysogenum Deacetoxy/deacetyl Cephalosporin C Synthase with Improved Activity toward Penicillin Analogs", FEMS MICROBIOLOGY LETTERS., vol. 246, no. 1, 8 April 2005 (2005-04-08), pages 103 - 110, XP027871731
HAN, SHU ET AL.: "Research Progress on Biosynthesis and Regulatory Mechanisms of Cephamycin C in Streptomyces Clavuligerus", CHINESE JOURNAL OF ANTIBIOTICS, vol. 40, no. 8, 31 August 2015 (2015-08-31), pages 561 - 569, XP055586704
WEI, C. L. ET AL.: "Directed Evolution of Streptomyces Clavuligerus Deacetoxycephalosporin C Synthase for Enhancement of Penicillin G Expansion", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 71, no. 12, 31 December 2005 (2005-12-31), pages 8873 - 8880, XP055450212
See also references of EP 3480313A4
Attorney, Agent or Firm:
TEE&HOWE INTELLECTUAL PROPERTY ATTORNEYS (CN)
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