Title:
CHIMERIC SIGNAL PEPTIDES FOR PROTEIN PRODUCTION
Document Type and Number:
WIPO Patent Application WO/2020/139855
Kind Code:
A3
Abstract:
A chimeric signal peptide for protein expression includes an N-region, a hydrophobic region, and a C-region, wherein the N-region and the C-region are from a same signal peptide of a first protein and the hydrophobic region is from a signal peptide of a second protein, wherein the first protein is different from the second protein. The first and second protein are independently selected from the group consisting of BM40, IL2, HA, Insulin, CD33, IFNA2, IgGK leader, AZU, and SEAP.
Inventors:
TENG CHAO-YI (TW)
CHEN YING-JU (TW)
CHEN YING-JU (TW)
Application Number:
PCT/US2019/068406
Publication Date:
July 30, 2020
Filing Date:
December 23, 2019
Export Citation:
Assignee:
DEV CT BIOTECHNOLOGY (CN)
DCB USA LLC (US)
DCB USA LLC (US)
International Classes:
C07K7/04; C07K14/17; C12N15/62
Other References:
SOOJIN HAN; SHUSHIL MACHHI; MARK BERGE; GUOLING XI; THOMAS LINKE; RONALD SCHONER: "Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli", AMB EXPRESS, vol. 7, no. 93, 12 May 2017 (2017-05-12) - 1 December 2017 (2017-12-01), pages 1 - 14, XP055683140
ZHANG, L ET AL.: "Alteration in the IL -2 signal peptide affects secretion of proteins in vitro and in vivo", THE JOURNAL OF GENE MEDICINE, vol. 7, no. 3, 13 December 2004 (2004-12-13), pages 354 - 365, XP003015830, DOI: 10.1002/jgm.677
PAUL HOLDEN, KEENE DOUGLAS R., LUNSTRUM GREGORY P., BÄCHINGER HANS PETER, HORTON WILLIAM A.: "Secretion of Cartilage Oligomeric Matrix Protein Is Affected by the Signal Peptide", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 280, no. 29, 3 March 2005 (2005-03-03), pages 17172 - 17179, XP055728479
ZHANG, L ET AL.: "Alteration in the IL -2 signal peptide affects secretion of proteins in vitro and in vivo", THE JOURNAL OF GENE MEDICINE, vol. 7, no. 3, 13 December 2004 (2004-12-13), pages 354 - 365, XP003015830, DOI: 10.1002/jgm.677
PAUL HOLDEN, KEENE DOUGLAS R., LUNSTRUM GREGORY P., BÄCHINGER HANS PETER, HORTON WILLIAM A.: "Secretion of Cartilage Oligomeric Matrix Protein Is Affected by the Signal Peptide", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 280, no. 29, 3 March 2005 (2005-03-03), pages 17172 - 17179, XP055728479
Attorney, Agent or Firm:
LIANG, T. Chyau et al. (US)
Download PDF: