Title:
CHROMATIN THREE-DIMENSIONAL CONFORMATION CAPTURE METHOD AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/012418
Kind Code:
A1
Abstract:
A chromatin three-dimensional conformation capture method, comprising: performing chromatin proximity ligation in situ, and performing spatial location information specific labelling on a proximity ligation product, such that chromatin three-dimensional conformation capture in the case of retaining spatial resolution is realized.
Inventors:
CHEN XUEPENG (CN)
CHEN ZHENPING (CN)
YU HAO (CN)
CHEN ZHENPING (CN)
YU HAO (CN)
Application Number:
PCT/CN2023/106632
Publication Date:
January 18, 2024
Filing Date:
July 10, 2023
Export Citation:
Assignee:
GUANGZHOU NAT LABORATORY (CN)
International Classes:
C12Q1/6869; C12Q1/6806; G16B20/20; G16B30/20; G16B50/00
Domestic Patent References:
| WO2017066907A1 | 2017-04-27 |
Foreign References:
| CN113466444A | 2021-10-01 | |||
| CN114891858A | 2022-08-12 |
Other References:
ZHANG, CHAO ET AL.: "tagHi-C Reveals 3D Chromatin Architecture Dynamics during Mouse Hematopoiesis", CELL REPORTS, vol. 32, 29 September 2020 (2020-09-29), XP055748789, DOI: 10.1016/j.celrep.2020.108206
DENG YANXIANG, BARTOSOVIC MAREK, MA SAI, ZHANG DI, KUKANJA PETRA, XIAO YANG, SU GRAHAM, LIU YANG, QIN XIAOYU, ROSOKLIJA GORAZD B.,: "Spatial profiling of chromatin accessibility in mouse and human tissues", NATURE, vol. 609, no. 7926, 8 September 2022 (2022-09-08), pages 375 - 383, XP093128375, ISSN: 0028-0836, DOI: 10.1038/s41586-022-05094-1
DENG YANXIANG, ZHANG DI, LIU YANG, SU GRAHAM, ENNINFUL ARCHIBALD, BAI ZHILIANG, FAN RONG: "Spatial Epigenome Sequencing at Tissue Scale and Cellular Level", BIORXIV, 12 March 2021 (2021-03-12), XP093128376, Retrieved from the Internet [retrieved on 20240207], DOI: 10.1101/2021.03.11.434985
DENG YANXIANG, BARTOSOVIC MAREK, MA SAI, ZHANG DI, LIU YANG, QIN XIAOYU, SU GRAHAM, XU MINA L., HALENE STEPHANIE, CRAFT JOSEPH E.,: "Spatial-ATAC-seq: spatially resolved chromatin accessibility profiling of tissues at genome scale and cellular level", BIORXIV, 7 June 2021 (2021-06-07), XP055908334, Retrieved from the Internet [retrieved on 20220404], DOI: 10.1101/2021.06.06.447244
DENG YANXIANG, BARTOSOVIC MAREK, MA SAI, ZHANG DI, KUKANJA PETRA, XIAO YANG, SU GRAHAM, LIU YANG, QIN XIAOYU, ROSOKLIJA GORAZD B.,: "Spatial profiling of chromatin accessibility in mouse and human tissues", NATURE, vol. 609, no. 7926, 8 September 2022 (2022-09-08), pages 375 - 383, XP093128375, ISSN: 0028-0836, DOI: 10.1038/s41586-022-05094-1
DENG YANXIANG, ZHANG DI, LIU YANG, SU GRAHAM, ENNINFUL ARCHIBALD, BAI ZHILIANG, FAN RONG: "Spatial Epigenome Sequencing at Tissue Scale and Cellular Level", BIORXIV, 12 March 2021 (2021-03-12), XP093128376, Retrieved from the Internet
DENG YANXIANG, BARTOSOVIC MAREK, MA SAI, ZHANG DI, LIU YANG, QIN XIAOYU, SU GRAHAM, XU MINA L., HALENE STEPHANIE, CRAFT JOSEPH E.,: "Spatial-ATAC-seq: spatially resolved chromatin accessibility profiling of tissues at genome scale and cellular level", BIORXIV, 7 June 2021 (2021-06-07), XP055908334, Retrieved from the Internet
Attorney, Agent or Firm:
IPSUNNY (CN)
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