Title:
CIRCRNA VACCINE AGAINST INFECTIOUS SPLEEN AND KIDNEY NECROSIS VIRUS, AND A CONSTRUCTION METHOD THEREFOR AND THE USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/123517
Kind Code:
A1
Abstract:
The present invention relates to a circRNA vaccine against an infectious spleen and kidney necrosis virus, and a construction method therefor and the use thereof. The method comprises: synthesizing a fusion sequence P-IRES-m6A-MCP with an in-vitro T7 (or S6) transcription promoter, an internal ribosome entry site IRES of an encephalomyocarditis virus, an N6-methyladenine (m6A) site, and an open reading frame of a major capsid protein (MCP) of an infectious spleen and kidney necrosis virus (ISKNV) sequentially at a 5' end, subjecting RNA to in-vitro transcription with a T7 (or S6) transcriptase by using P-IRES-m6A-MCP as a template, removing the DNA template with DNaseI, then performing linkage and cyclization with a T4 RNA ligase, removing linear RNA with a RNase R, and then obtaining circular RNA, i.e. circRNA-MCP. By means of the immunization with the circRNA-MCP, the incidence of infectious spleen and kidney necrosis can be reduced.
Inventors:
GONG CHENGLIANG (CN)
XUE RENYU (CN)
HU XIAOLONG (CN)
ZHU MIN (CN)
GU YUCHAO (CN)
FENG YONGJIE (CN)
XUE RENYU (CN)
HU XIAOLONG (CN)
ZHU MIN (CN)
GU YUCHAO (CN)
FENG YONGJIE (CN)
Application Number:
PCT/CN2021/144066
Publication Date:
July 06, 2023
Filing Date:
December 31, 2021
Export Citation:
Assignee:
UNIV SOOCHOW (CN)
International Classes:
C12N15/62; A61K39/12; A61P31/20
Domestic Patent References:
| WO2021041541A1 | 2021-03-04 |
Foreign References:
| CN113476597A | 2021-10-08 | |||
| US20190345503A1 | 2019-11-14 |
Other References:
OBI PRISCA; CHEN Y. GRACE: "The design and synthesis of circular RNAs", METHODS, ACADEMIC PRESS, NL, vol. 196, 2 March 2021 (2021-03-02), NL , pages 85 - 103, XP086875211, ISSN: 1046-2023, DOI: 10.1016/j.ymeth.2021.02.020
LEI, MING ET AL.: "Translation and functional roles of circular RNAs in human cancer", MOLECULAR CANCER, vol. 19, no. 1, 15 February 2020 (2020-02-15)
LEI, MING ET AL.: "Translation and functional roles of circular RNAs in human cancer", MOLECULAR CANCER, vol. 19, no. 1, 15 February 2020 (2020-02-15)
Attorney, Agent or Firm:
SUZHOU CREATOR PATENT AND TRADEMARK AGENCY, LTD (CN)
Download PDF:
Previous Patent: RESOURCE INDICATION METHOD, TERMINAL DEVICE, AND NETWORK DEVICE
Next Patent: DRYING DEVICE
Next Patent: DRYING DEVICE