Title:
CIS-REPLICON CONSTRUCT
Document Type and Number:
WIPO Patent Application WO/2022/166959
Kind Code:
A1
Abstract:
Provided is a new-type cis-replicon construct for efficiently expressing a target protein, which comprises an RNA polymerase coding unit and a target protein coding unit. The replicon construct can drive the replication of the target protein coding unit and reduce or avoid the replication and expression of the RNA polymerase, thereby effectively improving the expression of the target protein and reducing the expression of the non-target protein.
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Inventors:
ZHANG SHOUTAO (CN)
MA QIANG (CN)
TIAN QINGNAN (CN)
WANG TIAN (CN)
HUANG YONGHUI (CN)
LIU GUOTAO (CN)
TIAN ZHEN (CN)
MA QIANG (CN)
TIAN QINGNAN (CN)
WANG TIAN (CN)
HUANG YONGHUI (CN)
LIU GUOTAO (CN)
TIAN ZHEN (CN)
Application Number:
PCT/CN2022/075384
Publication Date:
August 11, 2022
Filing Date:
February 07, 2022
Export Citation:
Assignee:
UNIV ZHENGZHOU (CN)
International Classes:
C12N15/50; C12N15/11; C12N15/64; C12N15/86
Domestic Patent References:
| WO2002018585A2 | 2002-03-07 | |||
| WO2004085660A2 | 2004-10-07 |
Foreign References:
| CN112852841A | 2021-05-28 | |||
| US20140079734A1 | 2014-03-20 | |||
| CN202110151575A | 2021-02-03 | |||
| CN202111178173A | 2021-10-09 | |||
| CN109328233A | 2019-02-12 |
Other References:
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NORBERT PARDI ET AL., NAT REV DRUG DISCOV., vol. 17, 2018, pages 261 - 279
JENNIFER L. HYDE ET AL., VIRUS RESEARCH, vol. 206, 2015, pages 99 - 107
GIULIETTA MARUGGI ET AL., MOL THER., vol. 27, 2019, pages 757 - 772
KHROMYKH A ET AL., CURR OPIN MOL THER, vol. 2, 2000, pages 555 - 569
JESSE H. ERASMUS ET AL., MOL THER., vol. 26, 2018, pages 2507 - 2522
KENNETH LUNDSTROM, VIRUSES, vol. 6, 2014, pages 2392 - 2415
KENNETH LUNDSTROM, VACCINES, vol. 7, 2019, pages 29
CUILING ZHANG ET AL., FRONT IMMUNOL., vol. 10, 2019, pages 594
S GUANJ ROSENECKER, GENE THERAPY, vol. 24, 2017, pages 133 - 143
KRISTIE BLOOM ET AL., GENE THER., 2020, pages 1 - 13
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GOULD ET AL., ANTIVIRAL RES., vol. 87, 2010, pages 111 - 124
JONATHAN RUPP ET AL., J. GEN. VIROLOGY, vol. 96, 2015, pages 2483 - 2500
KATHRYN CARPENTIER ET AL., CURR OPIN VIROL, vol. 28, 2018, pages 53 - 60
FARHANA ABU BAKAR ET AL., VIRUSES, vol. 10, 2018, pages 105
AUTUMN HOLMES ET AL., PLOS PATHOG, vol. 16, 2020, pages e1008876
ERKUDEN CASALES ET AL., VIROLOGY, vol. 376, 2008, pages 242 - 251
TOEY NIVITCHANYONG ET AL., VIRUS RES., vol. 141, 2009, pages 1 - 12
AUTUMN T. LAPOINTE ET AL., MBIO, vol. 9, 2018, pages 02342 - 18
ELLEN G. STRAUSS ET AL., VIROLOGY, vol. 191, 1992, pages 932 - 940
M GOMEZ DE CEDRON ET AL., FEBS LETT, vol. 448, 1999, pages 19 - 22
TYLER LARK ET AL., FRONT MICROBIOL, vol. 8, 2017, pages 2652
KULASEGARAN SHYLINI ET AL., J VIROL., 2009, pages 8327 - 8339
JENNIFER L. HYDE ET AL., VIRUS RES, 2015, pages 99 - 107
KUHN ET AL., J VIROL, 1990, pages 1465 - 1476
HARDYRICE, J VIROL, vol. 79, 2005, pages 4630 - 4639
NADINE BANGEL-RULAND, J GENE MED, vol. 15, 2013, pages 414 - 426
ZELJKA TREPOTEC ET AL., RNA, vol. 25, 2019, pages 507 - 518
GAVIN HANSON ET AL., NAT REV MOL CELL BIOL., vol. 19, 2018, pages 20 - 30
VINCENT P MAURO ET AL., BIODRUGS, vol. 32, 2018, pages 69 - 81
HARDYRICE, J. VIROL., vol. 79, 2005, pages 4630 - 4639
KENNETH LUNDSTROM, MOLECULES, vol. 23, 2018, pages 3310
THOMAS DEMOULINS ET AL., NANOMEDICINE, vol. 12, 2016, pages 711 - 722
YASMIN GRANOT ET AL., SEMINARS IN IMMUNOLOGY, vol. 34, 2017, pages 68 - 77
PIOTR S. KOWALSKI ET AL., MOL THER, 2019, pages 710 - 728
DUBENSKY, T. W. ET AL.: "Sindbis virus DNA-based expression vectors: Utility for in vitro and in vivo gene transfer", JOURNAL OF VIROLOGY, vol. 70, no. 1, 1 January 1996 (1996-01-01), XP002039561, ISSN: 0022-538X
NORBERT PARDI ET AL., NAT REV DRUG DISCOV., vol. 17, 2018, pages 261 - 279
JENNIFER L. HYDE ET AL., VIRUS RESEARCH, vol. 206, 2015, pages 99 - 107
GIULIETTA MARUGGI ET AL., MOL THER., vol. 27, 2019, pages 757 - 772
KHROMYKH A ET AL., CURR OPIN MOL THER, vol. 2, 2000, pages 555 - 569
JESSE H. ERASMUS ET AL., MOL THER., vol. 26, 2018, pages 2507 - 2522
KENNETH LUNDSTROM, VIRUSES, vol. 6, 2014, pages 2392 - 2415
KENNETH LUNDSTROM, VACCINES, vol. 7, 2019, pages 29
CUILING ZHANG ET AL., FRONT IMMUNOL., vol. 10, 2019, pages 594
S GUANJ ROSENECKER, GENE THERAPY, vol. 24, 2017, pages 133 - 143
KRISTIE BLOOM ET AL., GENE THER., 2020, pages 1 - 13
STRAUSSSTRAUSS, MICROBIOL. REV., vol. 58, 1994, pages 491 - 562
GOULD ET AL., ANTIVIRAL RES., vol. 87, 2010, pages 111 - 124
JONATHAN RUPP ET AL., J. GEN. VIROLOGY, vol. 96, 2015, pages 2483 - 2500
KATHRYN CARPENTIER ET AL., CURR OPIN VIROL, vol. 28, 2018, pages 53 - 60
FARHANA ABU BAKAR ET AL., VIRUSES, vol. 10, 2018, pages 105
AUTUMN HOLMES ET AL., PLOS PATHOG, vol. 16, 2020, pages e1008876
ERKUDEN CASALES ET AL., VIROLOGY, vol. 376, 2008, pages 242 - 251
TOEY NIVITCHANYONG ET AL., VIRUS RES., vol. 141, 2009, pages 1 - 12
AUTUMN T. LAPOINTE ET AL., MBIO, vol. 9, 2018, pages 02342 - 18
ELLEN G. STRAUSS ET AL., VIROLOGY, vol. 191, 1992, pages 932 - 940
M GOMEZ DE CEDRON ET AL., FEBS LETT, vol. 448, 1999, pages 19 - 22
TYLER LARK ET AL., FRONT MICROBIOL, vol. 8, 2017, pages 2652
KULASEGARAN SHYLINI ET AL., J VIROL., 2009, pages 8327 - 8339
JENNIFER L. HYDE ET AL., VIRUS RES, 2015, pages 99 - 107
KUHN ET AL., J VIROL, 1990, pages 1465 - 1476
HARDYRICE, J VIROL, vol. 79, 2005, pages 4630 - 4639
NADINE BANGEL-RULAND, J GENE MED, vol. 15, 2013, pages 414 - 426
ZELJKA TREPOTEC ET AL., RNA, vol. 25, 2019, pages 507 - 518
GAVIN HANSON ET AL., NAT REV MOL CELL BIOL., vol. 19, 2018, pages 20 - 30
VINCENT P MAURO ET AL., BIODRUGS, vol. 32, 2018, pages 69 - 81
HARDYRICE, J. VIROL., vol. 79, 2005, pages 4630 - 4639
KENNETH LUNDSTROM, MOLECULES, vol. 23, 2018, pages 3310
THOMAS DEMOULINS ET AL., NANOMEDICINE, vol. 12, 2016, pages 711 - 722
YASMIN GRANOT ET AL., SEMINARS IN IMMUNOLOGY, vol. 34, 2017, pages 68 - 77
PIOTR S. KOWALSKI ET AL., MOL THER, 2019, pages 710 - 728
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW (CN)
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