Title:
COCAINE ESTERASE MUTANT AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/279816
Kind Code:
A1
Abstract:
Disclosed in the present invention are a cocaine esterase mutant and an application thereof. The cocaine esterase mutant is obtained by mutating a wild-type cocaine esterase, an amino acid sequence of the wild-type cocaine esterase being as shown in SEQ ID No. 1, the cocaine esterase mutant being T172R/G173Q/L196C/I301C, or with an additional V116K point mutation, or with an additional A51 point mutation, the A51 point mutation being to L, Y, V, F or W. Compared with the wild-type enzyme, the cocaine esterase mutant obtained by screening in the present invention has greatly improved catalytic efficiency for benzoylecgonine, a toxic metabolite of cocaine.
Inventors:
CHEN XIABIN (CN)
YAO JIANZHUANG (CN)
HOU SHURONG (CN)
DENG XINGYU (CN)
ZHANG YUN (CN)
TONG JUNSEN (CN)
YAO JIANZHUANG (CN)
HOU SHURONG (CN)
DENG XINGYU (CN)
ZHANG YUN (CN)
TONG JUNSEN (CN)
Application Number:
PCT/CN2022/089839
Publication Date:
January 12, 2023
Filing Date:
April 28, 2022
Export Citation:
Assignee:
UNIV HANGZHOU NORMAL (CN)
International Classes:
C12N9/18; A61K38/46; A61P39/02; B09C1/10; C02F3/34; C12N15/55
Domestic Patent References:
| WO2008008358A2 | 2008-01-17 |
Foreign References:
| CN113481182A | 2021-10-08 | |||
| CN101583374A | 2009-11-18 | |||
| CN101903038A | 2010-12-01 | |||
| AU2007272955A1 | 2008-01-17 | |||
| US20160122732A1 | 2016-05-05 | |||
| US20130039900A1 | 2013-02-14 |
Other References:
HUANG XIAOQIN, ZHAO XINYUN, ZHENG FANG, ZHAN CHANG-GUO: "Cocaine Esterase–Cocaine Binding Process and the Free Energy Profiles by Molecular Dynamics and Potential of Mean Force Simulations", JOURNAL OF PHYSICAL CHEMISTRY PART B, AMERICAN CHEMICAL SOCIETY, US, vol. 116, no. 10, 15 March 2012 (2012-03-15), US , pages 3361 - 3368, XP093022755, ISSN: 1520-6106, DOI: 10.1021/jp2111605
Attorney, Agent or Firm:
HANGZHOU JUNDU PATENT FIRM LLP (CN)
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