Title:
CODING SEQUENCE OF ND4 PROTEIN AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/077756
Kind Code:
A1
Abstract:
Disclosed are a nucleic acid encoding a human NADH dehydrogenase subunit 4 protein and an application thereof. The nucleotide sequence is shown in SEQ ID NO.: 1. Also disclosed is a fusion nucleic acid, comprising the nucleic acid encoding a human NADH dehydrogenase subunit 4 protein. Further disclosed is a recombinant expression vector, comprising the nucleic acid or fusion nucleic acid. Also disclosed is a transformant which introduces the nucleic acid or fusion nucleic acid into a host. The expression level of the nucleic acid encoding a human NADH dehydrogenase subunit 4 protein is higher, thus more human NADH dehydrogenase subunit 4 protein may be obtained in mitochondria, which may better treat Leber hereditary optic neuropathy.
Inventors:
LI BIN (CN)
Application Number:
PCT/CN2018/118662
Publication Date:
April 23, 2020
Filing Date:
November 30, 2018
Export Citation:
Assignee:
WUHAN NEUROPHTH BIOLOGICAL TECH LIMITED COMPANY (CN)
International Classes:
C12N15/62; A61K48/00; C12N15/85
Foreign References:
| JP2017121240A | 2017-07-13 | |||
| EP2913403A1 | 2015-09-02 | |||
| CN104450747A | 2015-03-25 | |||
| CN102634527A | 2012-08-15 |
Other References:
CWERMAN-THIBAULT, H. ET AL.: "Nuclear Expression of Mitochondrial Nd4 Leads to the Protein Assembling in Complex I and Prevents Optic Atrophy and Visual Loss", MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT., vol. 2, no. 15003, 25 February 2015 (2015-02-25), pages 1 - 15, XP055703863
WAN, X. ET AL.: "Efficacy and Safety of rAAV2-ND4 Treatment for Leber's Hereditary Optic Neuropathy", SCIENTIFIC REPORTS, vol. 6, no. 21587, 19 February 2016 (2016-02-19), pages 1 - 10, XP055687074
WAN, X. ET AL.: "Efficacy and Safety of rAAV2-ND4 Treatment for Leber's Hereditary Optic Neuropathy", SCIENTIFIC REPORTS, vol. 6, no. 21587, 19 February 2016 (2016-02-19), pages 1 - 10, XP055687074
Attorney, Agent or Firm:
METIS IP (CHENGDU) LLC (CN)
Download PDF: