Title:
COENZYME-LINKED GLUCOSE DEHYDROGENASE AND POLYNUCLEOTIDE ENCODING THE SAME
Document Type and Number:
WIPO Patent Application WO/2006/101239
Kind Code:
A1
Abstract:
[PROBLEMS] To provide means for the mass production of a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability against glucose and hardly acts on maltose. [MEANS FOR SOLVING PROBLEMS] A polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase which can catalyze the oxidation of glucose in the presence of an electron acceptor and has the degree of effect on maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting, from the resulting culture, a polypeptide which can link to FAD to exert the glucose dehydrating effect; a method for the determination of glucose using the polypeptide; a reagent composition for use in the determination of glucose; and a biosensor.
More Like This:
Inventors:
OMURA HIRONORI (JP)
SANADA HIROKAZU (JP)
YADA TAKAKO (JP)
ATSUMI AYAKA (JP)
MORITA TETSUNARI (JP)
ISHIMARU EMI (JP)
SANADA HIROKAZU (JP)
YADA TAKAKO (JP)
ATSUMI AYAKA (JP)
MORITA TETSUNARI (JP)
ISHIMARU EMI (JP)
Application Number:
PCT/JP2006/306198
Publication Date:
September 28, 2006
Filing Date:
March 27, 2006
Export Citation:
Assignee:
IKEDA FOOD RES CO LTD (JP)
OMURA HIRONORI (JP)
SANADA HIROKAZU (JP)
YADA TAKAKO (JP)
ATSUMI AYAKA (JP)
MORITA TETSUNARI (JP)
ISHIMARU EMI (JP)
OMURA HIRONORI (JP)
SANADA HIROKAZU (JP)
YADA TAKAKO (JP)
ATSUMI AYAKA (JP)
MORITA TETSUNARI (JP)
ISHIMARU EMI (JP)
International Classes:
C12N15/09; C12M1/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/04; C12Q1/32; C12R1/645; C12R1/66; C12R1/69
Domestic Patent References:
| WO2004058958A1 | 2004-07-15 | |||
| WO1998020136A1 | 1998-05-14 |
Foreign References:
| JP2001346587A | 2001-12-18 | |||
| JP2002223772A | 2002-08-13 | |||
| JP2001046078A | 2001-02-20 |
Other References:
KOJIMA S. ET AL.: "Fundamental study for an oxygen-insensitive amperometric glucose sensor using a novel glucose dehydrogenase", CHEMICAL SENSORS, vol. 20, no. SUPPLEMENT B, 11 July 2004 (2004-07-11), pages 768 - 769, XP003004574
KOJIMA N. ET AL.: "Shinki Glucose Dehydrogenase o Mochiita Sanso Fukan'no-sei Ketto Sensor no Kiso Kento (1)", THE JAPAN SOCIETY FOR ANALYTICAL CHEMISTRY NENKAI KOEN YOSHISHU, vol. 53, 18 August 2004 (2004-08-18), pages 80, XP003004575
INOSE K. ET AL.: "Cloning and expression of the gene encoding catalytic subunit of thermostable glucose dehydrogenase from Burkholderia cepacia in Escherichia coli", BIOCHIM. BIOPHYS. ACTA, vol. 1645, no. 2, 21 February 2003 (2003-02-21), pages 133 - 138, XP004405440
SODE K. ET AL.: "A novel thermostable glucose dehydrogenase varying temperature properties by altering its quaternary structures", ENZYME MICROB. TECHNOL., vol. 19, August 1996 (1996-08-01), pages 82 - 85, XP002970423
IWASHITA K. ET AL.: "Purification and characterization of extracellular and cell wall bound beta-glucosidases from Aspergillus kawachii", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 62, no. 10, October 1998 (1998-10-01), pages 1938 - 1946, XP003004576
TSUJIMURA S. ET AL.: "Novel FAD-Dependent Glucose Dehydrogenase for a Dioxygen-Insensitive Glucose Biosensor", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 70, no. 3, March 2006 (2006-03-01), pages 654 - 659, XP003004577
MERRIFIELD, R.B.: "J. Solid phase peptide synthesis I. The synthesis of tetrapeptide", J. AMER. CHEM. SOC., vol. 85, 1963, pages 2149 - 2154, XP002257754, DOI: doi:10.1021/ja00897a025
See also references of EP 1862543A1
KOJIMA N. ET AL.: "Shinki Glucose Dehydrogenase o Mochiita Sanso Fukan'no-sei Ketto Sensor no Kiso Kento (1)", THE JAPAN SOCIETY FOR ANALYTICAL CHEMISTRY NENKAI KOEN YOSHISHU, vol. 53, 18 August 2004 (2004-08-18), pages 80, XP003004575
INOSE K. ET AL.: "Cloning and expression of the gene encoding catalytic subunit of thermostable glucose dehydrogenase from Burkholderia cepacia in Escherichia coli", BIOCHIM. BIOPHYS. ACTA, vol. 1645, no. 2, 21 February 2003 (2003-02-21), pages 133 - 138, XP004405440
SODE K. ET AL.: "A novel thermostable glucose dehydrogenase varying temperature properties by altering its quaternary structures", ENZYME MICROB. TECHNOL., vol. 19, August 1996 (1996-08-01), pages 82 - 85, XP002970423
IWASHITA K. ET AL.: "Purification and characterization of extracellular and cell wall bound beta-glucosidases from Aspergillus kawachii", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 62, no. 10, October 1998 (1998-10-01), pages 1938 - 1946, XP003004576
TSUJIMURA S. ET AL.: "Novel FAD-Dependent Glucose Dehydrogenase for a Dioxygen-Insensitive Glucose Biosensor", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 70, no. 3, March 2006 (2006-03-01), pages 654 - 659, XP003004577
MERRIFIELD, R.B.: "J. Solid phase peptide synthesis I. The synthesis of tetrapeptide", J. AMER. CHEM. SOC., vol. 85, 1963, pages 2149 - 2154, XP002257754, DOI: doi:10.1021/ja00897a025
See also references of EP 1862543A1
Attorney, Agent or Firm:
Shiga, Masatake (Yaesu Chuo-ku, Tokyo 53, JP)
Download PDF: