Title:
COMBINATORIAL NUCLEOBASE OLIGOMERS COMPRISING UNIVERSAL BASE ANALOGUES AND METHODS FOR MAKING AND USING SAME
Document Type and Number:
WIPO Patent Application WO2004111072
Kind Code:
A3
Abstract:
The invention relates to insulating combinatorial nucleobase oligomers that comprise universal base analogs, where the oligomers are formed by the ligation of two or more oligomer "blocks" via a covalent linkage. Universal bases may serve to insulate specifically binding nucleobases from the effects of the covalent linker region joining two oligomer blocks together, so that the universal bases at least partially negate the Tm penalty caused by the covalent linkage, effective to reduce the required minimal length of the oligomer blocks and the combinatorial oligomer. The resulting insulating nucleobase combinatorial oligomers find use in any hybridization-based application, including use as probes and primers. The combinatorial oligomers of the present invention provide advantages over existing combinatorial oligomer systems currently known in the art.
Inventors:
LIVAK KENNETH J (US)
MULLAH K BASHAR (US)
MULLAH K BASHAR (US)
Application Number:
PCT/US2004/018800
Publication Date:
June 30, 2005
Filing Date:
June 10, 2004
Export Citation:
Assignee:
APPLERA CORP (US)
LIVAK KENNETH J (US)
MULLAH K BASHAR (US)
LIVAK KENNETH J (US)
MULLAH K BASHAR (US)
International Classes:
B01J19/00; C07H21/00; C07H21/04; C07K1/04; C07K14/00; C12N15/11; C12P19/34; C12Q1/68; C40B40/06; C40B50/00; (IPC1-7): C12Q1/68; C12N15/11; C07H21/04; C12P19/34; C07K1/04; C07K14/00; B01J19/00
Domestic Patent References:
| WO1999018238A1 | 1999-04-15 | |||
| WO2000002899A1 | 2000-01-20 |
Other References:
LOAKES D: "SURVEY AND SUMMARY THE APPLICATIONS OF UNIVERSAL DNA BASE ANALOGUES", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 29, no. 12, 15 June 2001 (2001-06-15), pages 2437 - 2447, XP001179827, ISSN: 0305-1048
SEELA FRANK ET AL: "The N8-(2'-deoxyribofuranoside) of 8-aza-7-deazaadenine: A universal nucleoside forming specific hydrogen bonds with the four canonical DNA constituents", NUCLEIC ACIDS RESEARCH, vol. 28, no. 17, 1 September 2000 (2000-09-01), pages 3224 - 3232, XP002323814, ISSN: 0305-1048
NICHOLS R ET AL: "A UNIVERSAL NUCLEOSIDE FOR USE AT AMBIGOUS SITES IN DNA PRIMERS", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 369, no. 6480, 9 June 1994 (1994-06-09), pages 492 - 493, XP000560346, ISSN: 0028-0836
SEELA FRANK ET AL: "The N8-(2'-deoxyribofuranoside) of 8-aza-7-deazaadenine: A universal nucleoside forming specific hydrogen bonds with the four canonical DNA constituents", NUCLEIC ACIDS RESEARCH, vol. 28, no. 17, 1 September 2000 (2000-09-01), pages 3224 - 3232, XP002323814, ISSN: 0305-1048
NICHOLS R ET AL: "A UNIVERSAL NUCLEOSIDE FOR USE AT AMBIGOUS SITES IN DNA PRIMERS", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 369, no. 6480, 9 June 1994 (1994-06-09), pages 492 - 493, XP000560346, ISSN: 0028-0836
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