Title:
COMPETITIVE N-HYBRID SYSTEM
Document Type and Number:
WIPO Patent Application WO2006117225
Kind Code:
B1
Abstract:
The invention relates to a method for identifying highly affinous ligands comprising the following steps: a) creating a bank for a mutagenized first hybrid protein comprising a plurality of mutants; b) expression of a first hybrid protein in a host with a second hybrid protein, one of the hybrid proteins comprising the DNA binding domain of a transcription factor and a bait protein and the other hybrid protein comprising the activation domain for a transcription factor and a prey protein; c) allowing the binding reaction between the first and second hybrid protein in order to form a complex with a functional transcription factor in the host cell under reaction conditions, which are such that the balance of the binding reaction is offset on the side of the hybrid proteins; d) detecting the binding reaction by detecting a reporter gene expressed via the functional transcription factor; e) optionally repeating a step or several steps from a) to d); f) selection of a mutant.
Inventors:
ARNTZ CLAUDIA (DE)
MEINDERS DANIELLE (DE)
BLOCK CHRISTOPH (DE)
MEINDERS DANIELLE (DE)
BLOCK CHRISTOPH (DE)
Application Number:
PCT/EP2006/004190
Publication Date:
October 25, 2007
Filing Date:
May 04, 2006
Export Citation:
Assignee:
SIGNALOMICS GMBH (AT)
ARNTZ CLAUDIA (DE)
MEINDERS DANIELLE (DE)
BLOCK CHRISTOPH (DE)
ARNTZ CLAUDIA (DE)
MEINDERS DANIELLE (DE)
BLOCK CHRISTOPH (DE)
International Classes:
C12N15/10; G01N33/68
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