Title:
COMPOSITIONS AND METHODS FOR HIGHLY EFFICIENT GENETIC SCREENING USING BARCODED GUIDE RNA CONSTRUCTS
Document Type and Number:
WIPO Patent Application WO/2020/125762
Kind Code:
A1
Abstract:
Compositions, kits and methods are provided for genetic screening using one or more sets of guide RNA constructs having internal barcodes ("iBAR"). Each set has three or more guide RNA constructs targeting the same genomic locus, but embedded with different iBAR sequences.
Inventors:
WEI WENSHENG (CN)
ZHU SHIYOU (CN)
CAO ZHONGZHENG (CN)
LIU ZHIHENG (CN)
HE YUAN (CN)
YUAN PENGFEI (CN)
ZHU SHIYOU (CN)
CAO ZHONGZHENG (CN)
LIU ZHIHENG (CN)
HE YUAN (CN)
YUAN PENGFEI (CN)
Application Number:
PCT/CN2019/127080
Publication Date:
June 25, 2020
Filing Date:
December 20, 2019
Export Citation:
Assignee:
UNIV BEIJING (CN)
EDIGENE BIOTECHNOLOGY INC (CN)
EDIGENE BIOTECHNOLOGY INC (CN)
International Classes:
C12N15/66; C12N9/22; C12N15/10; C12N15/113; C40B40/06
Domestic Patent References:
| WO2017214460A1 | 2017-12-14 | |||
| WO2016205745A2 | 2016-12-22 | |||
| WO2018154027A1 | 2018-08-30 |
Foreign References:
| CN106062197A | 2016-10-26 | |||
| CN107090466A | 2017-08-25 | |||
| CN106232823A | 2016-12-14 |
Other References:
See also references of EP 3898983A4
Attorney, Agent or Firm:
BEIJING CAI HE LAW FIRM (CN)
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