PLANT DISEASE

CROSS-REFERENCE TO RELATED APPLICATIONS This application claims priority to U.S. Provisional Application Nos. 63/190,047, filed May 18, 2021, and 63/257,208, filed October 19, 2021, each of which is incorporated by reference herein in its entirety.

FIELD OF THE INVENTION

The invention relates to a bacterial strain and variants thereof and populations for controlling plant pests and disease and/or improving an agronomic trait of interest in a plant.

BACKGROUND

Plant diseases and plant pests are responsible for significant agricultural losses. Effects can range from mild symptoms to catastrophic plant damage, which can lead to major economic and social consequences. Methods are needed to effectively control plant diseases and the pathogens that cause them and to effectively control plant pests.

SUMMARY

Compositions and methods for controlling plant diseases and plant pests and/or for improving at least one agronomic trait of interest in a plant are provided. Such compositions and methods comprise a population of biocontrol agents or bacterial strains that control one or more pathogens that cause plant disease, one or more plant pests, and/or improve at least one agronomic trait of interest. The biological agents or bacterial strains can be used as an inoculant for plants. Methods for growing a plant susceptible to plant disease and methods and compositions for controlling plant disease and plant pests are also provided. Further provided are methods and compositions of increasing disease resistance and pest resistance in plants. Methods and compositions for improving plant health and/or improving at least one agronomic trait of interest are also provided.

DETAILED DESCRIPTION

I. Overview

Compositions and methods for improving at least one agronomic trait of interest and/or improving plant health and/or for controlling one or more plant diseases and/or plant pests are provided. A biological agent, biocontrol agent, bacterial strain, modified bacterial strain, modified biological agent, or modified biocontrol agent or active variant therof, and/or a composition derived therefrom are used herein to describe a microorganism that is used to control plant pests, disease-causing plant pathogens and/or improve at least one agronomic trait of interest and/or improve plant health. The biocontrol agent can be used alone or in combination with another biocontrol agent known in the art or disclosed herein. II Bacterial Strains

The biocontrol agent or bacterial strain AIP015329, AIP075655, AIP059286, or a variant of any thereof can be used to control one or more plant pest, one or more plant disease, and/or improve at least one agronomic trait of interest and/or improve plant health. Cell populations comprising AIP015329, AIP075655, OR AIP059286 are provided, as well as populations of spores derived from this strain, or any preparation thereof. The AIP015329, AIP075655, or AIP059286 bacterial strains and/or the pesticidal compositions provided herein comprise as an active ingredient a cell population comprising or an active variant of any thereof.

Bacterial strain AIP075655 is described in WO 2020/077042, incorporated by reference herein, and was deposited with the Patent Depository of the National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604 U.S.A. on August 3, 2018 and assigned NRRL No. B-67651. The deposit will be maintained under the terms of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure. The deposit was made merely as a convenience for those of skill in the art and is not an admission that a deposit is required under 35 U.S. C. §112.

Bacterial strain AIP059286 is described in WO 2020/092381, incorporated by reference herein, and was deposited with the Patent Depository of the National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604 U.S.A. on August 3, 2018 and assigned NRRL No. B-67653. The deposit will be maintained under the terms of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure. The deposit was made merely as a convenience for those of skill in the art and is not an admission that a deposit is required under 35 U.S. C. §112.

Bacterial strain AIP015329 was deposited with the Patent Depository of the National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604 U.S.A. on March 25, 2019 and assigned NRRL No. B-67754. The deposit will be maintained under the terms of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure. The deposit was made merely as a convenience for those of skill in the art and is not an admission that a deposit is required under 35 U.S.C. §112.

The term "isolated" encompasses a bacterium, spore, or other entity or substance, that has been (1) separated from at least some of the components with which it was associated when initially produced (whether in nature or in an experimental setting), and/or (2) produced, prepared, purified, and/or manufactured by the hand of man. Isolated bacteria may be separated from at least about 10%, about 20%, about 30%, about 40%, about 50%, about 60%, about 70%, about 80%, about 90%, or more of the other components with which they were initially associated. As used herein, a substance is "pure" if it is substantially free of other components. The terms "purify," "purifying" and "purified" zrefer to a bacterium, spore, or other material that has been separated from at least some of the components with which it was associated either when initially produced or generated (e.g., whether in nature or in an experimental setting), or during any time after its initial production. A bacterium or spore or a bacterial population or a spore population may be considered purified if it is isolated at or after production, such as from a material or environment containing the bacterium or bacterial population or spore, and a purified bacterium or bacterial population or spore may contain other materials up to about 10%, about 20%, about 30%, about 40%, about 50%, about 60%, about 70%, about 80%, about 90%, or above about 90% and still be considered purified. In some embodiments, purified bacteria or spores and bacterial populations or spore populations are more than about 80%, about 85%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99%, or more than about 99% pure. In specific embodiments, a culture of bacteria contains no other bacterial species in quantities to be detected by normal bacteriological techniques.

In some embodiments, the compositions of the invention comprise substantially pure cultures of bacterial strain AIP015329, AIP075655, or AIP059286. The compositions of the invention also provide progeny of substantially pure cultures of bacterial strain AIP015329, AIP075655, or AIP059286, wherein the culture has all of the physiological and morphological characteristics of AIP015329, AIP075655, or AIP059286.

By "population" is intended a group or collection that comprises two or more individuals ^. e., 10, 100, 1,000, 10,000, 1x10 ⁶ , 1x10 ⁷ , or 1x10 ⁸ or greater) of a given bacterial strain. Various compositions are provided herein that comprise a population of at least one bacterial strain or a mixed population of individual poplations from more than one bacterial strain. In specific embodiments, the population of bacterial strain AIP015329, AIP075655, or AIP059286, or an active variant of any thereof, or spores or forespores or a combination of cells, forespores and/or spores, and/or a composition derived from AIP015329, AIP075655, or AIP059286 or an active variant of any thereof comprises a concentration of at least about 10 ³ CFU/ml to about 10 ⁵ CFU/ml, 10 ³ CFU/ml to about 10 ⁴ CFU/ml, 10 ³ CFU/ml to about 10 ⁶ CFU/ml, 10 ⁴ CFU/ml to about 10 ⁸ CFU/ml, 10 ⁵ CFU/ml to about 10 ¹¹ CFU/ml, about 10 ⁵ CFU/ml to about 10 ¹⁰ CFU/ml, about 10 ⁵ CFU/ml to about 10 ¹² CFU/ml, about 10 ⁵ CFU/ml to about 10 ⁶ CFU/ml, about 10 ⁶ CFU/ml to about 10 ⁷ CFU/ml, about 10 ⁷ CFU/ml to about 10 ⁸ CFU/ml, about 10 ⁸ CFU/ml to about 10 ⁹ CFU/ml, about 10 ⁹ CFU/ml to about 10 ¹⁰ CFU/ml, about 10 ¹⁰ CFU/ml to about 10 ¹¹ CFU/ml, about 10 ¹¹ CFU/ml to about 10 ¹² CFU/ml. In other embodiments, the concentration of the AIP015329, AIP075655, or AIP059286 bacterial strain or an active variant of any thereof and/or a composition derived therefrom comprises at least about 10 ⁵ CFU/ml, at least about 10 ⁶ CFU/ml, at least about 10 ⁷ CFU/ml, at least about 10 ⁸ CFU/ml, at least about 10 ⁹ CFU/ml, at least about 10 ¹⁰ CFU/ml, at least about 10 ¹¹ CFU/ml, or at least about 10 ¹² CFU/ml. In particular embodiments, the population of AIP015329, AIP075655, or AIP059286 or an active variant of any thereof comprises a concentration of at least about 10 ³ CFU/g to about 10 ⁴ , 10 ³ CFU/g to about 10 ⁵ CFU/g, CFU/g, 10 ³ CFU/g to about 10 ⁶ CFU/g, 10 ⁴ CFU/g to about 10 ⁸ CFU/g, 10 ⁵ CFU/g to about 10 ¹¹ CFU/g, about 10 ⁵ CFU/g to about 10 ¹⁰ CFU/g, about 10 ⁵ CFU/g to about 10 ¹² CFU/g, about 10 ⁵ CFU/g to about 10 ⁶ CFU/g, about 10 ⁶ CFU/g to about 10 ⁷ CFU/g, about 10 ⁷ CFU/g to about 10 ⁸ CFU/g, about 10 ⁸ CFU/g to about 10 ⁹ CFU/g, about 10 ⁹ CFU/g to about 10 ¹⁰ CFU/g, about 10 ¹⁰ CFU/g to about 10 ¹¹ CFU/g, about 10 ¹¹ CFU/g to about 10 ¹² CFU/g. In other embodiments, the concentration of the AIP015329, AIP075655, or AIP059286 bacterial strain or active variant of any thereof comprises at least about 10 ² CFU/g, at least about 10 ³ CFU/g, at least about 10 ⁴ CFU/g, at least about 10 ⁵ CFU/g, at least about 10 ⁶ CFU/g, at least about 10 ⁷ CFU/g, at least about 10 ⁸ CFU/g, at least about 10 ⁹ CFU/g, at least about 10 ¹⁰ CFU/g, at least about 10 ¹¹ CFU/g, or at least about 10 ¹² CFU/g. The bacterial concentration of a given solid or liquid composition or formulation can be expressed in CFU/g or CFU/mU, respectively, or expressed as activity or viability using any methods described herein. For example, a measure bacterial viability that is equivalent to CFU can be expressed in terms of cells/g or cells/mU if using epifluorescent measurements or a measure of activity can be expressed as pg of a metabolite, such as pyrrolnitrin, per g of bacteria when using metabolite measurements as a reporter metabolite, such as for example using pyrrolnitrin as a reporter metabolite.

A “spore” refers to at least one dormant (at application) but viable reproductive unit of a bacterial species. Non-limiting methods by which spores are formed from AIP015329 or variants thereof are disclosed elsewhere herein. It is further recognized the populations disclosed herein can comprise a combination of vegetative cells and forespores (cells in an intermediate stage of spore formation); a combination of forespores and spores; or a combination of forespores, vegetative cells and/or spores.

As used herein, “derived from” means directly isolated or obtained from a particular source or alternatively having identifying characteristics of a substance or organism isolated or obtained from a particular source. In the event that the “source” is an organism, “derived from” means that it may be isolated or obtained from the organism itself or a culture broth, suspension, or medium used to culture or grow said organism. A compound or composition “derived from” or “obtainable from” means that the compound or composition may be isolated from or produced by a cell culture or a whole cell broth, or a suspension, filtrate, supernatant, fraction, or extract derived from a cell culture or a whole cell broth.

As used herein, “whole broth culture” or “whole cell broth” refers to a liquid culture containing both cells and media. If bacteria are grown on a plate, the cells can be harvested in water or other liquid, whole culture. The terms “whole broth culture” and “whole cell broth” are used interchangeably.

As used herein, “supernatant” refers to the liquid remaining when cells grown in broth or are harvested in another liquid from an agar plate and are removed by centrifugation, filtration, _^ sedimentation, or other means well known in the art. In some embodiments, the supernatant may be diluted with another composition, such as water, buffer, fresh media, and/or a formulation. The diluted supernatant is still considered a supernatant of the invention.

As used herein, “fdtrate” refers to liquid from a whole broth culture that has passed through a membrane. The fdtrate may comprise a concentrated amount of an effective compound or metabolite compared to the concentration of the effective compound or metabolite in the whole broth culture or supernatant. As used herein, “extract” refers to liquid substance removed from cells by a solvent (water, detergent, buffer, and/or organic solvent, for example) and separated from the cells by centrifugation, fdtration, or other method known in the art. The extract may comprise a concentrated amount of an effective compound or metabolite compared to the concentration of the effective compound or metabolite in the cells prior to extraction. Alternatively, the filtrate or extract may then be diluted with another composition, such as water, buffer, fresh media, and/or a formulation. Such diluted filtrates or extracts are still considered filtrates and extracts of the invention.

As used herein, “metabolite” refers to a compound, substance, or byproduct of fermentation of a bacterial strain. An effective compound or metabolite is a compound present in the supernatant, whole cell broth, or bacterial strain which may improve any agronomic trait of interest of a plant, or which controls a plant pest or a plant pathogen that causes a plant disease, when applied to a plant of interest at an effective amount.

In some embodiments, a composition of the invention comprises a filtrate or extract derived from fermentation of the AIP015329, AIP075655, or AIP059286 bacterial strain or an active variant of any thereof, wherein said composition comprises a concentrated amount of an effective compound or metabolite compared to the amount in a whole cell broth or supernatant of said bacterial strain. In other embodiments, a composition of the invention comprises a diluted filtrate, diluted extract, or diluted supernatant derived from the fermentation of the AIP015329, AIP075655, or AIP059286 bacterial strain or an active variant of any thereof, wherein said composition comprises a diluted amount of the effective compound or metabolite compared to the amount whole cell broth or undiluted supernatant of said bacterial strain. The diluted filtrate, diluted extract, or diluted supernatant may still comprise an effective amount of the effective compound or metabolite.

The compositions and methods described herein comprise or are derived from AIP015329, AIP075655, or AIP059286 or an active variant of any thereof, or a spore or a forespore or a combination of cells, forespores or/and spores, from AIP015329, AIP075655, or AIP059286 or an active variant of any thereof. Methods also comprise cultivating bacterial strain AIP015329, AIP075655, or AIP059286 or an active variant of any thereof. In some embodiments, bacterial strain AIP015329, AIP075655, or AIP059286 or an active variant of any thereof is cultivated and compounds and/or compositions are obtained by isolating these compounds and/or compositions from the culture of AIP015329, AIP075655, or AIP059286 or an active variant of any thereof.

In some embodiments, bacterial strain AIP015329, AIP075655, or AIP059286 or an active variant of any thereof is cultivated in nutrient medium using methods known in the art. The bacterial strain can be cultivated by shake flask cultivation or by small scale or large scale fermentation (including but not limited to continuous, batch, fed-batch, or solid state fermentation) in laboratory or industrial fermenters performed in a suitable medium and under conditions allowing for bacterial cell growth. The cultivation can take place in suitable nutrient medium comprising carbon and nitrogen sources and inorganic salts, using prodedures known in the art. Suitable media are available from commercial sources or are prepared according to publications well-known in the art. Following cultivation, compounds, metabolites, and/or compositions can be extracted from the culture broth. The extract can be fractionated by chromatography. The extract can be further purified using methods well-known in the art. The extract can also be diluted using methods well-known in the art.

The compositions comprising bacterial strain AIP015329, AIP075655, or AIP059286 or an active variant of any thereof, or a spore or a forespore or a combination of cells, forespores and/or spores, and/or a composition derived from AIP015329, AIP075655, or AIP059286 or an active variant of any thereof can further comprise an agriculturally acceptable carrier. The term "agriculturally acceptable carrier" is intended to include any material that facilitates application of a composition to the intended subject (i.e, a plant or plant part susceptible to a plant pest or plant disease of interest (i.e., powdery mildew), or any other pest or disease disclosed herein or a plant or plant part for improving an agronomic trait of interest. Carriers used in compositions for application to plants and plant parts are preferably non-phytotoxic or only mildly phytotoxic. A suitable carrier may be a solid, liquid or gas depending on the desired formulation. In one embodiment, carriers include polar or non-polar liquid carriers such as water, mineral oils and vegetable oils. Additional carriers are disclosed elsewhere herein.

A. Active Variants of a Bacterial Strain

Further provided are active variants of AIP015329, AIP075655, or AIP059286. Such variants will retain the ability to control one or more plant diseases (i.e., reduce disease severity and/or reduce disease development), and/or control one or more plant pests (e.g., bacterial pests, fungal and fungal-like pests, nematode pests, insect pests). In some embodiments, variants will retain the ability to control one or more fungal plant diseases and/or one or more fungal pathogens. In other embodiments, variants will retain the ability to control sorghum anthracnose, late blight, powdery mildew, black sigatoka, and/or Aspergillus spp. In further embodiments, variants will retain the ability to control Colletrotrichum spp., Phytophthora spp., Podosphaera spp., Aspergillus spp., and/or Mycosphaerella spp. In still further embodiments, variants will retain the ability to control Colletotrichum sublineolum, Phytophthora infestans, Podosphaera xanthii, Aspergillus flavus, and/ or Mycosphaerella fijiensis. In some embodiments, variants will retain all of the physiological and morphological characteristics of the parent bacterial strain disclosed herein. Active variants of the various bacterial strains provided herein include, for example, any isolate or mutant of AIP015329, AIP075655, or AIP059286 which retains the ability to control plant diseases.

As used herein, “pesticidal activity” refers to activity against one or more pests, including fungi, fungal-like pathogens (e.g., Oomycetes, plasmodiophorids, and the Phytomyxea), bacteria, insects, nematodes, viruses, viroids, protozoan pathogens, and the like, such that the pest is killed or controlled. In some embodiments, variants will retain the ability to control one or more insect pests or nematode pests. In particular embodiments, variants will retain the ability to control fungal and fungal-like pests, including Oomycetes such as Pythium, Phytophthora, and downy mildews. As used herein downy mildews include but are not limited to pathogenic species of the genera Peronospora, Pseudoperonospora, Bremia,

Plasmopara, and Basidiophora. The term “mutant” refers to a variant of the parental stran as well as methods for obtaining a mutant or variant in which the pesticidal activity is greater than that expressed by the parental strain. The “parent strain” is the original strain before mutagenesis. To obtain such mutants the parental strain may be treated with a chemical such as N-methyl-N’-nitro-N-nitrosoguanidine, ethylmethanesulfone (EMS), or by irradiation using gamma, x-ray, or UV -irradiation, or by other means well known in the art.

In some embodiments, the mutant may be the result of a spontaneous mutation which generates a phenotype. Such a mutant may be derived from an AIP015329, AIP075655, or AIP059286 population when grown continuously in liquid culture. The spontaneous mutation may be a naturally occurring mutation or an induced mutation. In other embodiments, the spontaneous mutation may be derived from an AIP015329, AIP075655, or AIP059286 population when grown continuously on a solid media, such as an agar plate. These spontaneous mutants are considers derivatives of the bacterial strain of the invention. Said derivatives may be derived from the deposited strain. In some embodiments, derivatives retain all of the physiological and morphological characteristics of the bacterial strain of the invention. In some embodiments, derivatives retain the ability to control sorghum anthracnose, late blight, powdery mildew, black sigatoka, and/or Aspergillus spp. In further embodiments, derivatives will retain the ability to control Colletrotrichum spp., Phytophthora spp., Podosphaera spp., Aspergillus spp., and/or Mycosphaerella spp. In still further embodiments, derivatives will retain the ability to control Colletotrichum sublineolum, Phytophthora infe stans, Podosphaera xanthii, Aspergillus flavus, an d/o r Mycosphae re l la fijiensis.

In some embodiments, the variant or derivative contains a mutation in at least one gene, relative to the deposited strain. The gene(s) may have a role in, for example, biofdm formation, motility, chemotaxis, extracellular secretion, transport (for example ABC transporter proteins), stress responses, volatiles, transcription (for example alternative sigma factors and global transcription regulators), root colonization, ability to stimulate induced systemic resistance in a plant, and/or secondary metabolism including synthesis of lipopeptides, polyketides, macromolecular hydrolases (for example proteases and/or carbohydrases), and/or antimicrobial compounds including antibiotics. Secondary metabolism refers to both non-ribosomal and ribosomal synthesis of antimicrobial compounds, including cyclic lipopeptides, polyketides, iturins, bacteriocins (for example plantazolicin and amylocyclicin) and dipeptides (for example bacilysin).

An example of a variant is a cell of bacterial strain disclosed herein, wherein the cell further comprises a mutation in the swrA gene that results in loss of function. The swrA mutation, which affects biofdm formation (Keams et al, Molecular Microbiology (2011) 52(2): 357-369) may result in a variant of a strain of the invention which has enhanced ability to control a plant pest or improve an agronomic trait of interest of a plant. Other genes that are involved in biofdm formation, such as sfp, epsC, degQ, and a plasmid gene called rapP (see for example, McLoon et al, J of Bacteriology, (2011) 193(8): 2027-2034), may also be mutated in an active variant of a bacterial strain of the invention.

In specific embodiments, the bacterial strain is compatible with a biocide. A biocide is a chemical substance that can exert a controlling effect on an organism by chemical or biological means. Biocides include pesticides, such as fungicides; herbicides; insecticides, other crop protection chemicals, and the like. Such compounds are discussed in detail elsewhere herein. A bacterial strain is compatible with a biocide when the bacterial strain is able to survive and/or reproduce in the presence of an effective amount of a biocide of interest. In instances where the bacterial strain is not compatible for a biocide of interest, if desired, methods can be undertaken to modify the bacterial strain to impart the compatibility of interest.

Such methods to produce modified bacterial strains include both selection techniques and/or transformation techniques.

By “modified bacterial strain” is intended a population wherein the strain has been modified (by selection and/or transformation) to have one or more additional traits of interest. In some embodiments the modified bacterial strain is an active variant of AIP015329, AIP075655, or AIP059286. In specific embodiments, the modified bacterial strain is compatible with a biocide of interest, including but not limited to, resistance to a herbicide, fungicide, pesticide, or other crop protection chemical. The modified biocide- resistant strains have the same identification characteristics as the original sensitive strain except they are significantly more resistant to the particular herbicide, fungicide, pesticide, or other crop protection chemical. Their identification is readily possible by comparison with characteristics of the known sensitive strain. Thus, isolated populations of modified bacterial strains are provided.

An increase in resistance to a biocide (i.e., for example, a herbicide, fungicide, pesticide, or other crop protection chemical resistance) refers to the ability of an organism (i.e., bacterial cell or spore) to survive and reproduce following exposure to a dose of the biocide (e.g, herbicide, fungicide, pesticide, or other crop protection chemical) that would normally be lethal to the unmodified organism or would substantially reduce growth of the unmodified organism. In specific embodiments, the increase in resistance to a biocide is demonstrated in the presence of an agriculturally effective amount of the biocide.

In such instances, the modified bacterial strain having resistance to one or more biocides is useful for enhancing the competitiveness of bacterial strains particularly over other microbial agents which are not resistant to herbicides, fungicides, pesticides, or other crop protection chemicals. Therefore, compositions provided herein include selected or engineered bacterial strains and modified populations of bacterial strains. These bacterial strains or modified bacterial strains can be used as an inoculant for plants. They can also be applied as a spray application directly to the aerial parts of plants, and can be mixed with the herbicide or other chemical to which they have been modified to become tolerant.

Thus, active variants of AIP015329, AIP075655, or AIP059286 include a modified strain, such that the active variant controls a plant disease and further is able to grow in the presence of at least one biocide.

Recombinant bacterial strains having resistance to an herbicide, fungicide, pesticide, or other crop protection chemical can be made through genetic engineering techniques and such engineered or recombinant bacterial strains may be grown to produce a modified population of bacterial strains. A recombinant bacterial strain is produced by introducing polynucleotides into the bacterial host cell by transformation. Methods for transforming microorganisms are known and available in the art. See, generally, Hanahan, D. (1983) Studies on transformation of Escherichia coli with plasmids J. Mol. Biol. 166, 557-77; Seidman, C.E. (1994) In: Current Protocols in Molecular Biology, Ausubel, F.M. etal. eds., John Wiley and Sons, NY; Choi et al. (2006) J. Microbiol. Methods 64:391-397; Wang et al. 2010. J. Chem. Technol. Biotechnol. 85:775-778. Transformation may occur by natural uptake of naked DNA by competent cells from their environment in the laboratory . Alternatively, cells can be made competent by exposure to divalent cations under cold conditions, by electroporation, by exposure to polyethylene glycol, by treatment with fibrous nanoparticles, or other methods well known in the art.

Herbicide resistance genes useful in transforming a bacterial strain include, but are not limited to, fumonisin detoxification genes (U.S. Patent No. 5,792,931); acetolactate synthase (ALS) mutants that lead to herbicide resistance, in particular the sulfonylurea-type herbicides, such as the S4 and/or Hra mutations; inhibitors of glutamine synthase such as phosphinothricin or basta (e.g., bar gene); and glyphosate resistance (EPSPS gene); gluphosinate, and HPPD resistance (WO 96/38576, U.S. Patent Nos. 6,758,044; 7,250,561; 7,935,869; and 8,124,846), or other such genes known in the art. The disclosures of WO 96/38576, U.S. Patent No. 5,792,931, U.S. Patent No. 6,758,044; U.S. Patent No. 7,250,561; U.S. Patent No. 7,935,869; and U.S. Patent No. 8,124,846 are herein incorporated by reference. The bar gene encodes resistance to the herbicide basta, the npt\\ gene encodes resistance to the antibiotics kanamycin and geneticin, and the AUS- gene mutants encode resistance to the sulfonylurea herbicides including chlorsulfuron, metsulfuron, sulfometuron, nicosulfuron, rimsulfuron, flazasulfuron, sulfosulfuron, and triasulfuron, and the imadizolinone herbicides including imazethapyr, imazaquin, imazapyr, and imazamethabenz.

To identify and produce a modified population of bacterial strains through selection, the bacterial strains are grown in the presence of the herbicide, fungicide, pesticide, or other crop protection chemical as the selection pressure. Susceptible agents are killed while resistant agents survive to reproduce without competition. As the bacterial strains are grown in the presence of the herbicide, fungicide, pesticide, or other crop protection chemical, resistant bacterial strains successfully reproduce and become dominant in the population, becoming a modified population of bacterial strains. Methods for selecting resistant strains are known and include U.S. Patent Nos. 4,306,027 and 4,094,097, herein incorporated by reference. The active variant of the bacterial strain comprising a modified population of bacterial strains will have the same identification characteristics as the original sensitive strain except they are significantly more tolerant to the particular herbicide, fungicide, pesticide, or other crop protection chemical. Thus, their identification is readily possible by comparison with characteristics of the known sensitive strain.

Further active variants of the various bacteria provide herein can be identified employing, for example, methods that determine the sequence identity relatedness between the 16S ribosomal RNA, methods to identify groups of derived and functionally identical or nearly identical strains include Multilocus sequence typing (MUST), concatenated shared genes trees, Whole Genome Alignment (WGA), Average Nucleotide Identity, and MinHash (Mash) distance metric.

In one aspect, the active variants of a bacterial strain disclosed herein include strains that are closely related to said bacterial strain by employing the Bishop MUST method of organism classification as defined in Bishop et al. (2009) BMC Biology 7(1)1741-7007-7-3. Thus, in specific embodiments, an active variant of the bacterial strain AIP015329, AIP075655, or AIP059286 includes a bacterial strain that falls within at least a 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%. 94%, 95%, 96%, 97%, 98%, 98.5%,

98.8%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, or 99.9% sequence cut off employing the Bishop method of organism classification as set forth in Bishop el al. (2009) BMC Biology 7(1)1741-7007-7-3, which is herein incorporated by reference in its entirety. Active variants of the bacteria identified by such methods will retain the ability to improve at least one agronomic trait when applied in an effective amount to a plant, plant part, or an area of cultivation, including for example, reducing plant disease severity, reducing plant disease development, increasing plant resistance, increasing plant health, and/or improving an agronomic trait of interest in a plant.

In another aspect, the active variant of the bacterial strain(s) disclosed herein include strains that are closely related to any of the disclosed strains on the basis of the Average Nucleotide Identity (ANI) method of organism classification. ANI (see, for example, Konstantinidis, K.T., et al, (2005) PNAS USA 102(7):2567-72; and Richter, M., et al., (2009) PNAS 106(45): 19126-31) and variants (see, for example, Varghese, N.J., et al. , Nucleic Acids Research (July 6, 2015): gkv657) are based on summarizing the average nucleotides shared between the genomes of strains that align in WGAs. Thus, in specific embodiments, an active variant of bacterial strain AIP015329, AIP075655, or AIP059286 disclosed herein includes a bacterial stain that falls within at least a 90%, 95%, 96%, 97%, 97.5%, 98%, 98.5%, 98.8%, 99%, 99.5%, or 99.8% sequence cut off employing the ANI method of organism classification as set forth in Konstantinidis, K.T., et al, (2005) PNAS USA 102(7):2567-72, which is herein incorporated by reference in its entirety. Active variants of the bacteria identified by such methods will retain the ability to improve at least one agronomic trait when applied in an effective amount to a plant, plant part, or an area of cultivation, including for example, reducing plant disease severity and/or , reducing plant disease development, and/or increasing plant resistance to a pest.

In another aspect, the active variants of the isolated bacterial strains disclosed herein includes strains that are closely related on the basis of 16S rDNA sequence identity. See Stackebrandt E. et al., “Report of the ad hoc committee tor the re-evaluation of the species definition in bacteriology A Int JSyst Evol Microbiol. 52(3): 1043-7 (2002) regarding use of 16S rDNA sequence identity for determining relatedness in bacteria. In an embodiment, the at least one strain is at least 95% identical to any of the above strains on the basis of 16S rDN A sequence identity, at least 96% identical to any of the abo ve strains on the basis of 16S rDNA sequence identity, at least 97% identical to any of the above strains on the basis of 16 S rDNA sequence identity, at least 98% to any of the above strains on the basis of 16S rDNA sequence identity, at least 98.5% identical to any of the above strains on the basis of 16S rDNA sequence identity, at least 99% identical to any of the above strains on the basis of 16S rDNA sequence identity, at least 99.5% to any of the above strains on the basis of 16S rDN A sequence i dentity or at least 100% to any of the a bove strai ns on the basis of 16S rDNA sequence identity. Active variants of the bacteria identified by such methods will retain the ability to improve at least one agronomic trait when applied in an effective amount to a plant, plant part, or an area of cultivation, including for example, reducing plant disease severity , increasing plant resistance to a pest, and/or reducing plant disease development. The MinHash (Mash) distance metric is a comparison method that defines thresholds for hierarchical classification of microorganisms at high resolution and requires few parameters and steps (Ondov et al. (2016) Genome Biology 17: 132). The Mash distance estimates the mutation rate between two sequences directly from their MinHash sketches (Ondov et al. (2016) Genome Biology 17: 132). Mash distance strongly corresponds to Average Nucleotide Identity method (ANI) for hierarchical classification (See, Konstantinidis, K.T. et al. (2005) PNAS USA 102(7):2567-72, herein incorporated by reference in its entirety). That is, an ANI of 97% is approximately equal to a Mash distance of 0.03, such that values put forth as useful classification thresholds in the ANI literature can be directly applied with the Mash distance.

Active variants of the bacterial strain AIP015329, AIP075655, or AIP059286 include strains that are closely related on the basis of the Minhash (Mash) distance between complete genome DNA sequences. Thus, in specific embodiments, an active variant of a bacterial strain disclosed herein includes bacterial strains having a genome within a Mash distance of less than about 0.015 to the disclosed strains. In other embodiments, an active variant of a bacterial strain disclosed herein includes a distance metric of less than about 0.001, 0.0025 ,_0.005, 0.010, 0.015, 0.020, 0.025, or 0.030. A genome as it relates to the Mash distance includes both bacterial chromosomal DNA and bacterial plasmid DNA. In other embodiments, the active variant of a bacterial strain has a genome that is above a Mash distance threshold to the disclosed strains that is greater than dissimilarity caused by technical variance. In further instances, the active variant of a bacterial strain has a genome that is above a Mash distance threshold to the disclosed strains that is greater than dissimilarity caused by technical variance and has a Mash distance of less than about 0.015. In other instances, the active variant of a bacterial strain has a genome that is above a Mash distance threshold to the disclosed strains that is greater than dissimilarity caused by technical variance and has a Mash distance of less than about 0.001, 0.0025, 0.005, 0.010, 0.015, 0.020, 0.025, or 0.030.

As used herein, “above technical variation” means above the Mash distance between two strains caused by errors in the genome assemblies provided the genomes being compared were each DNA sequenced with at least 20X coverage with the Illumina HiSeq 2500 DNA sequencing technology and the genomes are at least 99% complete with evidence for contamination of less than 2%. While 20X coverage is an art recognized term, for clarity, an example of 20X coverage is as follows: for a genome size of 5 megabases (MB), 100 MB of DNA sequencing from the given genome is required to have 20X sequencing coverage on average at each position along the genome. There are many suitable collections of marker genes to use for genome completeness calculations including the sets found in Campbell et al. (2013) PNAS USA 110(14):5540-45, Dupont et al. (2012) ISMEJ 6: 1625- 1628. and the CheckM framework (Parks et al. (2015) Genome Research 25:1043-1055); each of these references is herein incorporated in their entirety. Contamination is defined as the percentage of typically single copy marker genes that are found in multiple copies in the given genome sequence (e.g. Parks et al. (2015) Genome Research 25:1043-1055); each of these references is herein incorporated in their entirety. Completeness and contamination are calculated using the same collection of marker genes. Unless otherwise stated, the set of collection markers employed in the completeness and contamination assay is the set forth in Campbell el al. (2013) PNAS USA 110(14): 5540-45, herein incorporated by reference.

Exemplary steps to obtain a distance estimate between the genomes in question are as follows: (1) Genomes of sufficient quality for comparison must be produced. A genome of sufficient quality is defined as a genome assembly created with enough DNA sequence to amount to at least 20X genome coverage using Illumina HiSeq 2500 technology. The genome must be at least 99% complete with contamination of less than 2% to be compared to the claimed microbe’s genome. (2) Genomes are to be compared using the Minhash workflow as demonstrated in Ondov et al. (2016) Genome Biology 17:132, herein incorporated by reference in its entirety. Unless otherwise stated, parameters employed are as follows: “sketch” size of 1000, and “k-mer length” of 21. (3) Confirm that the Mash distance between the 2 genomes is less than 0.001, 0.0025, 0.005, 0.010, 0.015, 0.020, 0.025, or 0.030. Using the parameters and methods stated above, a Mash distance of 0.015 between two genomes means the expected mutation rate is 0.015 mutations per homologous position. Active variants of the bacteria identified by such methods will retain the ability to improve at least one agronomic trait when applied in an effective amount to a plant, plant part, or an area of cultivation, including for example, reducing plant disease severity, reducing plant disease development, and/or increasing plant resistance to a pest.

III. Formulations

The bacteria strains provided herein (i.e., AIP015329, AIP075655, AIP059286, or an active variant of any thereof, or a spore or a forespore or a combination of cells, forespores, and/or spores, and/or a composition derived from AIP015329, AIP075655, AIP059286, or an active variant of any thereof) can be formulated as a cell paste, wettable powders, a cell pellet, dusts, granules, a slurry, a dry powder, aqueous or oil based liquid products, and the like. Such formulations will comprise the bacteria provided herein or an active variant thereof, and/or a composition derived therefrom in addition to carriers and other agents suitable for use in the methods as disclosed elsewhere herein, including but not limited to contacting a pest to control it, or applying to a plant, plant part, seed or area of cultivation in order to control a plant pest or treat or prevent a plant disease.

The bacterial strain AIP015329, AIP075655, or AIP059286 bacterial strain or active variants of any thereof can be formulated to include at least one or more of an extender, a solvent, spontaneity promoter, carrier, emulsifier, dispersant, frost protectant, thickener, and/or adjuvant. In some embodiments, the extender, solvent, spontaneity promoter, carrier, emulsifier, dispersant, frost protectant, thickener, and/or adjuvant is a non-natural or synthetic extender, a solvent, spontaneity promoters, carriers, emulsifiers, dispersants, frost protectants, thickeners, and/or adjuvants. In particular embodiments, the bacterial strains disclosed herein and the active variants thereof can be formulated to include at least one or more natural extender, a solvent, spontaneity promoter, carrier, emulsifier, dispersant, frost protectant, thickener, and/or adjuvant. Examples of typical formulations include water-soluble liquids (SL), emulsifiable concentrates (EC), emulsions in water (EW), suspension concentrates (SC), suspo-emulsions (SE), flowable concentrates for seed treatment (FS), oil dispersions (OD), water-dispersible granules (WG), granules (GR), capsule concentrates (CS), water-dispersible granules (WG), granules (GR), block baits (BB), water-soluble granules (SG), and mixed formulations of CS and SC (ZC). In some embodiments, the formulation may be a waxy coating. These and other possible types of formulation are described, for example, by Crop Life International and in Pesticide Specifications, Manual on development and use of FAO and WHO specifications for pesticides, FAO Plant Production and Protection Papers - 173, prepared by the FAO/WHO Joint Meeting on Pesticide Specifications, 2004, ISBN: 9251048576. The formulations may comprise active agrochemical compounds other than one or more active compounds of the invention.

The formulations or application forms of the various bacterial strains or active variants thereof can comprise, but are not limited to, auxiliaries, such as extenders, solvents, spontaneity promoters, carriers, emulsifiers, dispersants, frost protectants, biocides, solid carriers, surfactants, thickeners and/or other auxiliaries, such as adjuvants. An adjuvant in this context is a component which enhances the biological effect of the formulation, without the component itself having a biological effect. Examples of adjuvants are agents which promote the retention, spreading, attachment to the leaf surface, or penetration.

Non-limiting extenders are, for example, water, polar and nonpolar organic chemical liquids, for example from the classes of the aromatic and non-aromatic hydrocarbons (such as paraffins, alkyl benzenes, alkylnaphthalenes, chlorobenzenes), the alcohols and polyols (which, if appropriate, may also be substituted, etherified and/or esterified), the ketones (such as acetone, cyclohexanone), esters (including fats and oils) and (poly)ethers, the unsubstituted and substituted amines, amides, lactams (such as N-alkylpyrrolidones) and lactones, the sulphones and sulphoxides (such as dimethyl sulphoxide). If the extender used is water, it is also possible to employ, for example, organic solvents as auxiliary solvents. Essentially, non-limiting liquid solvents are: aromatics such as xylene, toluene or alkylnaphthalenes, chlorinated aromatics and chlorinated aliphatic hydrocarbons such as chlorobenzenes, chloroethylenes or methylene chloride, aliphatic hydrocarbons such as cyclohexane or paraffins, for example petroleum fractions, mineral and vegetable oils, alcohols such as butanol or glycol and also their ethers and esters, ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone or cyclohexanone, strongly polar solvents such as dimethylformamide and dimethyl sulphoxide, and also water. In principle it is possible to use any suitable solvent. Non-limiting solvents are, for example, aromatic hydrocarbons, such as xylene, toluene or alkylnaphthalenes, for example, chlorinated aromatic or aliphatic hydrocarbons, such as chlorobenzene, chloroethylene or methylene chloride, for example, aliphatic hydrocarbons, such as cyclohexane, for example, paraffins, petroleum fractions, mineral and vegetable oils, alcohols, such as methanol, ethanol, isopropanol, butanol or glycol, for example, and also their ethers and esters, ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone or cyclohexanone, for example, strongly polar solvents, such as dimethyl sulphoxide, and water.

Non-limiting examples of suitable carriers include, for example, ammonium salts and ground natural minerals such as kaolins, clays, talc, chalk, quartz, attapulgite, montmorillonite or diatomaceous earth, and ground synthetic minerals, such as finely divided silica, alumina and natural or synthetic silicates, resins, waxes and/or solid fertilizers. Mixtures of such carriers may likewise be used. Carriers suitable for granules include the following: for example, crushed and fractionated natural minerals such as calcite, marble, pumice, sepiolite, dolomite, and also synthetic granules of inorganic and organic meals, and also granules of organic material such as sawdust, paper, coconut shells, maize cobs, and tobacco stalks.

Liquefied gaseous extenders or solvents may also be used. Non-limiting examples are those extenders or carriers which at standard temperature and under standard pressure are gaseous, examples being aerosol propellants, such as halogenated hydrocarbons, and also butane, propane, nitrogen and carbon dioxide. Examples of emulsifiers and/or foam-formers, dispersants or wetting agents having ionic or nonionic properties, or mixtures of these surface-active substances, are salts of polyacrylic acid, salts of lignosulphonic acid, salts of phenolsulphonic acid or naphthalene sulphonic acid, poly condensates of ethylene oxide with fatty alcohols or with fatty acids or with fatty amines, with substituted phenols (preferably alkylphenols or arylphenols), salts of sulphosuccinic esters, taurine derivatives (preferably alkylta urates), phosphoric esters of polyethoxylated alcohols or phenols, fatty acid esters of polyols, and derivatives of the compounds containing sulphates, sulphonates and phosphates, examples being alkylaryl polyglycol ethers, alkylsulphonates, alkyl sulphates, arylsulphonates, protein hydrolysates, lignin-sulphite waste liquors and methylcellulose. The presence of a surface -active substance is advantageous if one of the active compounds and/or one of the inert carriers is not soluble in water and if application takes place in water.

Further auxiliaries that may be present in the formulations and in the application forms derived from them include colorants such as inorganic pigments, examples being iron oxide, titanium oxide, Prussian Blue, and organic dyes, such as alizarin dyes, azo dyes and metal phthalocyanine dyes, and nutrients and trace nutrients, such as salts of iron, manganese, boron, copper, cobalt, molybdenum, and zinc.

Stabilizers, such as low-temperature stabilizers, preservatives, antioxidants, light stabilizers or other agents which improve chemical and/or physical stability may also be present. Additionally present may be foam-formers or defoamers.

Furthermore, the formulations and application forms derived from them may also comprise, as additional auxiliaries, stickers such as carboxymethylcellulose, natural and synthetic polymers in powder, granule or latex form, such as gum arabic, polyvinyl alcohol, polyvinyl acetate, and also natural phospholipids, such as cephalins and lecithins, and synthetic phospholipids. Further possible auxiliaries include mineral and vegetable oils.

There may possibly be further auxiliaries present in the formulations and the application forms derived from them. Examples of such additives include fragrances, protective colloids, binders, adhesives, thickeners, thixotropic substances, penetrants, retention promoters, stabilizers, sequestrants, complexing agents, humectants and spreaders. Generally speaking, the active compounds may be combined with any solid or liquid additive commonly used for formulation purposes. Suitable retention promoters include all those substances which reduce the dynamic surface tension, such as dioctyl sulphosuccinate, or increase the viscoelasticity, such as hydroxypropylguar polymers, for example.

Suitable penetrants in the present context include all those substances which are typically used in order to enhance the penetration of active agrochemical compounds into plants. Penetrants in this context are defined in that, from the (generally aqueous) application liquor and/or from the spray coating, they are able to penetrate the cuticle of the plant and thereby increase the mobility of the active compounds in the cuticle. This property can be determined using the method described in the literature (Baur et al., 1997, Pesticide Science 51: 131-152). Examples include alcohol alkoxylates such as coconut fatty ethoxylate (10) or isotridecyl ethoxylate (12), fatty acid esters such as rapeseed or soybean oil methyl esters, fatty amine alkoxylates such as tallowamine ethoxylate (15), or ammonium and/or phosphonium salts such as ammonium sulphate or diammonium hydrogen phosphate, for example.

The various compositions and formulations disclosed herein can comprise an amount of the bacterial strain AIP015329, AIP075655, or AIP059286 or an active variant of any thereof, or a spore or a forespore or a combination of cells, forespores or/and spores, and/or can comprise an amount of a composition derived from AIP015329, AIP075655, or AIP059286 or an active variant of any thereof. In certain embodiments, the viability of a composition comprising the bacterial strain AIP015329, AIP075655, or AIP059286 or an active variant of any thereof can be quantitated by measuring the number of colony forming units per gram or per ml of the composition. In particular embodiments, the composition comprises a concentration of the bacterial strain of at least about 10 ⁴ CFU/gram to about 10 ¹¹ CFU/gram, at least about 10 ⁵ CFU/gram to about 10 ¹¹ CFU/gram, about 10 ⁵ CFU/gram to about 10 ¹⁰ CFU/gram, about 10 ⁵ CFU/gram to about 10 ¹² CFU/gram, about 10 ⁵ CFU/gram to about 10 ⁶ CFU/gram, about 10 ⁶ CFU/gram to about 10 ⁷ CFU/gram, about 10 ⁷ CFU/gram to about 10 ⁸ CFU/gram, about 10 ⁸ CFU/gram to about 10 ⁹ CFU/gram, about 10 ⁹ CFU/gram to about 10 ¹⁰ CFU/gram, about 10 ¹⁰ CFU/gram to about 10 ¹¹ CFU/gram, or about 10 ¹¹ CFU/gram to about 10 ¹² CFU/gram. In other embodiments, the concentration of the bacterial strain comprises at least about 10 ⁵ CFU/gram, at least about 10 ⁶ CFU/gram, at least about 10 ⁷ CFU/gram, at least about 10 ⁸ CFU/gram, at least about 10 ⁹ CFU/gram, at least about 10 ¹⁰ CFU/gram, at least about 10 ¹¹ CFU/gram, at least about 10 ¹² CFU/gram, at least about 10 ⁴ CFU/gram. Such concentrations of the bacterial strain can occur in any formulation type of interest, including, for example in a liquid formulation, wettable power, spray dried formulation, cell paste, wettable granule, or freeze-dried formulation.

In some embodiments, the bacterial strain can occur in a liquid formulation. Fiquid formulations can comprise an amount of a cell of the bacterial strain AIP015329, AIP075655, or AIP059286 or an active variant of any thereof, or a spore or a forespore or a combination of cells, forespores and/or spores from AIP015329, AIP075655, or AIP059286 or an active variant of any thereof, and/or a composition derived therefrom. In liquid formulations, the amount of bacterial strain or active variant thereof, and/or a composition derived therefrom, disclosed herein can comprise a concentration of at least about 10 ⁴ to about 10 ¹¹ CFU/mF, at least about 10 ⁵ CFU/mF to about 10 ¹¹ CFU/ mF, about 10 ⁵ CFU/ mF to about 10 ¹⁰ CFU/ mL, about 10 ⁵ CFU/ mL to about 10 ¹² CFU/ mL, about 10 ⁵ CFU/ mL to about 10 ⁶ CFU/ mL, about 10 ⁶ CFU/ mL to about 10 ⁷ CFU/ mL, about 10 ⁷ CFU/ mL to about 10 ⁸ CFU/ mL, about 10 ⁸ CFU/ mL to about 10 ⁹ CFU/ mL, about 10 ⁹ CFU/ mL to about 10 ¹⁰ CFU/ mL, about 10 ¹⁰ CFU/ mL to about 10 ¹¹ CFU/ mL, or about 10 ¹¹ CFU/ mL to about 10 ¹² CFU/ mL or at least about 10 ⁴ CFU/ mL, at least about 10 ⁵ CFU/ mL, at least about 10 ⁶ CFU/ mL, at least about 10 ⁷ CFU/ mL, at least about 10 ⁸ CFU/ mL, at least about 10 ⁹ CFU/ mL, at least about 10 ¹⁰ CFU/ mL, at least about 10 ¹¹ CFU/ mL, at least about 10 ¹² CFU/ mL.

Dry formulations such as cell pastes, wettable powders, granules, and spray dried formulations can comprise the bacterial strain AIP015329, AIP075655, or AIP059286 or an active variant of any thereof, or a spore or a forespore or a combination of cells, forespores or/and spores of any thereof, and/or can comprise a composition derived from AIP015329, AIP075655, or AIP059286 or an active variant of any thereof. The amount of the bacterial strain in the cell paste or wettable powder can comprise a concentration of the bacterial strain of at least about 10 ⁵ CFU/gram to about 10 ¹¹ CFU/gram, about 10 ⁷ CFU/gram to about 10 ¹⁰ CFU/gram, about 10 ⁷ CFU/gram to about 10 ¹¹ CFU/gram, about 10 ⁶ CFU/gram to about 10 ¹⁰ CFU/gram, about 10 ⁶ CFU/gram to about 10 ¹¹ CFU/gram, about 10 ¹¹ CFU/gram to about 10 ¹² CFU/gram, about 10 ⁵ CFU/gram to about 10 ¹⁰ CFU/gram, about 10 ⁵ CFU/gram to about 10 ¹² CFU/gram, about 10 ⁵ CFU/gram to about 10 ⁶ CFU/gram, about 10 ⁶ CFU/gram to about 10 ⁷ CFU/gram, about 10 ⁷ CFU/gram to about 10 ⁸ CFU/gram, about 10 ⁸ CFU/gram to about 10 ⁹ CFU/gram, about 10 ⁹ CFU/gram to about 10 ¹⁰ CFU/gram, about 10 ¹⁰ CFU/gram to about 10 ¹¹ CFU/gram, or about 10 ¹¹ CFU/gram to about 10 ¹² CFU/gram. In some embodiments, the concentration of the bacterial strain comprises at least about 10 ⁵ CFU/gram, at least about 10 ⁶ CFU/gram, at least about 10 ⁷ CFU/gram, at least about 10 ⁸ CFU/gram, at least about 10 ⁹ CFU/gram, at least about 10 ¹⁰ CFU/gram, at least about 10 ¹¹ CFU/gram, at least about 10 ¹² CFU/gram, or at least about 10 ¹³ CFU/gram.

As used herein, a “cell paste” comprises a population of cells that has been centrifuged and/or fdtered and/or otherwise concentrated.

Further provided is a coated seed which comprises a seed and a coating on the seed, wherein the coating comprises at least one bacterial strain such as AIP015329, AIP075655, or AIP059286 or an active variant of any thereof, or a spore or a forespore or a combination of cells, forespores or/and spores, and/or can comprise a composition derived from AIP015329, AIP075655, or AIP059286 or an active variant of any thereof, wherein said bacterial strain or the active variant thereof is present on the seed at about 10 ⁵ CFU/ 100 lbs of seed to about 10 ¹⁰ CFU/ 100 lbs of seed, at about 10 ⁶ CFU/ 100 lbs of seed to about 10 ¹¹ CFU/100 lbs of seed, at about 10 ⁸ CFU/100 lbs of seed to about 10 ⁹ CFU/100 lbs of seed, at about 10 ⁸ CFU/100 lbs of seed to about 10 ¹⁰ CFU/100 lbs of seed, at about 10 ⁶ CFU/100 lbs of seed to about 10 ¹¹ CFU/100 lbs of seed, or at about 10 ⁷ CFU/100 lbs of seed to about 10 ¹¹ CFU/100 lbs of seed. In some embodiments, said bacterial strain or the active variant thereof is present on the seed at about 10 ⁷ cells/ 100 lbs of seed to about 10 ¹² cells/100 lbs of seed, at about 10 ⁸ cells/100 lbs of seed to about 10 ¹³ cells/100 lbs of seed, at about 10 ¹⁰ cells/100 lbs of seed to about 10 ¹¹ cells/100 lbs of seed, at about 10 ⁷ cells/100 lbs of seed to about 10 ¹⁴ cells/100 lbs of seed, at about 10 ⁸ cells/100 lbs of seed to about 10 ¹⁰ cells/100 lbs of seed, at about 10 ⁹ cells/100 lbs of seed to about 10 ¹³ cells/100 lbs of seed, at about 10 ⁸ cells/100 lbs of seed to about 10 ¹³ cells/100 lbs of seed, at about 10 ⁸ cells/100 lbs of seed to about 10 ¹⁴ cells/100 lbs of seed, or at about 10 ⁷ cells/100 lbs of seed to about 10 ¹⁴ cells/100 lbs of seed. Various plants of interest are disclosed elsewhere herein.

A seed coating can further comprise at least at least one nutrient, at least one herbicide or at least one pesticide, or at least one biocide. See, for example, US App Pub. 20040336049, 20140173979, and 20150033811.

In other embodiments, the viability of the bacterial strain AIP015329, AIP075655, or AIP059286 or an active variant of any thereof in a composition or formulation can be quantitated using an epifluorescence assay in which fluorescent dyes that are specific for cells with intact membranes or disrupted membranes are utilized, such as those assays that use a SYTO 9 nucleic acid stain that fluoresces green indicating a cell has an intact membrane and propidium iodide that fluoresces red indicating a cell with a disrupted membrane that is not viable (see, for example, LIVE/DEAD ^® Bac Light™ Bacterial Viability and Counting Kit from Molecular Probes; and Ivanova et al. (2010) Biotechnology & Biotechnological Equipment 24:supl, 567- 570). For Bacillus spp., it has been shown in the art that dormant spores do not stain readily with either SYT09 or propidium iodide, however dead dormant spores will stain red (Ghosh et al. (2017) Scientific Reports 7: 17768). Intact Bacillus spp. spores are also readily detected as phase bright in phase contrast microsopy.

It is known that following desiccation, some Pseudomonas strains enter a metabolically active state in which the cells are viable but not culturable (VBNC) (Pazos-Rojas et al. (2019) PLoS ONE 14(7):e0219554). Cells in a VBNC state retain the ability to be cultured if reconstituted, for example, in water or root exudates, when exposed to particular metals or ions, or any other reconstitution method that is specific for the individual VBNC bacterial strain.

In some embodiments, the composition or formulation comprises a concentration (e.g., as measured by viability) of the bacterial strain of at least about 10 ¹ cells/gram to about 10 ¹⁵ cells/gram, where “cells” may include viable cells, live dormant spores, forespores, and germinating spores. In some embodiments, the composition or formulation comprises a concentration of the bacterial strain of 10 ² cells/gram to about 10 ⁵ cells/gram, 10 ² cells/gram to about 10 ⁴ cells/gram, 10 ³ cells/gram to about 10 ⁶ cells/gram, 10 ⁴ cells/gram to about 10 ⁸ cells/gram, at least about 10 ⁵ cells/gram to about 10 ¹¹ cells/gram, about 10 ⁷ cells/gram to about 10 ¹¹ cells/gram, about 10 ⁷ cells/gram to about 10 ¹³ cells/gram, about 10 ⁶ cells/gram to about 10 ¹¹ cells/gram, about 10 ⁶ cells/gram to about 10 ¹³ cells/gram, about 10 ¹⁰ cells/gram to about 10 ¹² cells/gram, about 10 ⁸ cells/gram to about 10 ¹³ cells/gram, about 10 ⁹ cells/gram to about 10 ¹⁴ cells/gram, about 10 ⁸ cells/gram to about 10 ¹² cells/gram, about 10 ⁸ cells/gram to about 10 ¹² cells/gram, about 10 ⁹ cells/gram to about 10 ¹² cells/gram, about 10 ¹⁰ cells/gram to about 10 ¹¹ cells/gram, about 10 ¹¹ cells/gram to about 10 ¹² cells/gram, about 10 ¹¹ cells/gram to about 10 ¹² cells/gram, or about 10 ¹² cells/gram to about 10 ¹³ cells/gram, where “cells” may include viable cells, live dormant spores, forespores, and germinating spores. In some embodiments, the concentration of the bacterial strain comprises at least about 10 ² cells/gram, at least about 10 ³ cells/gram, at least about 10 ⁴ cells/gram, at least about 10 ⁵ cells/gram, at least about 10 ⁶ cells/gram, at least about 10 ⁷ cells/gram, at least about 10 ⁸ cells/gram, at least about 10 ⁹ cells/gram, at least about 10 ¹⁰ cells/gram, at least about 10 ¹¹ cells/gram, at least about 10 ¹² cells/gram, at least about 10 ¹³ cells/gram, at least about 10 ¹⁴ cells/gram, or at least about 10 ¹⁵ cells/gram, where “cells” may include viable cells, live dormant spores, forespores, and germinating spores as measured with an epifluorescence assay and/or phase contrast microscopy.

In liquid compositions and formulations, the amount of bacterial strain, or active variant thereof, disclosed herein can comprise a concentration of at least about 10 ¹ cells/mL to about 10 ¹⁵ cells/mL, where “cells” may include viable cells, live dormant spores, forespores, and germinating spores. In some embodiments, the composition or formulation comprises a concentration of the bacterial strain of 10 ² cells/mL to about 10 ⁶ cells/mL, 10 ⁵ cells/mL to about 10 ¹⁰ cells/mL, 10 ⁸ cells/mL to about 10 ¹⁵ cells/mL, 10 ⁹ cells/mL to about 10 ¹² cells/mL, at least about 10 ³ to about 10 ⁹ cells/mL, at least about 10 ³ to about 10 ⁶ cells/mL, at least about 10 ⁴ to about 10 ¹¹ cells/mL, at least about 10 ⁸ cells/mL to about 10 ¹³ cells/mL, about 10 ⁵ cells/mL to about 10 ¹⁰ cells/mL, about 10 ⁵ cells/mL to about 10 ¹² cells/mL, about 10 ⁸ cells/mL to about 10 ¹⁵ cells/mL, about 10 ⁸ cells/mL to about 10 ¹² cells/mL, about 10 ⁷ cells/mL to about 10 ¹¹ cells/mL, about 10 ⁸ cells/mL to about 10 ¹¹ cells/mL, about 10 ⁹ cells/mL to about 10 ¹⁰ cells/mL, about 10 ¹⁰ cells/mL to about 10 ¹¹ cells/mL, or about 10 ¹¹ cells/mL to about 10 ¹² cells/mL or at least about 10 ³ cells/mL, at least about 10 ⁴ cells/mL, at least about 10 ⁵ cells/mL, at least about 10 ⁶ cells/mL, at least about 10 ⁷ cells/mL, at least about 10 ⁸ cells/mL, at least about 10 ⁹ cells/mL, at least about 10 ¹⁰ cells/mL, at least about 10 ¹¹ cells/mL, at least about 10 ¹² cells/mL, at least about 10 ¹³ cells/mL, at least about 10 ¹⁴ cells/mL, or at least about 10 ¹⁵ cells/mL, where “cells” may include viable cells, live dormant spores, forespores, and germinating spores as measured with an epifluorescence assay and/or phase contrast microscopy.

It is known that bacterial strains with pesticidal activity produce a battery of secondary metabolites which can serve as antibacterial and antifungal compounds (Lucke et al. (2020) Frontiers in Plant Science 11: Article 589416). In some embodiments, the concentration of a secondary metabolite within a composition or formulation comprising a bacterial strain, such as AIP015329, AIP075655, or AIP059286 or an active variant of any thereof, can be measured as a surrogate of the viability and/or pesticidal activity of the bacterial strain in the composition or formulation. For example, pyrrolnitrin can be measured as a reporter metabolite for antifungal activity as it is co-regulated with other antifungal metabolites that are active in Pseudomonas spp. The presence of pyrrolnitrin is a measure of intact cells and cell concentration within a composition or formulation. Pyrrolnitrin and other antifungal metabolites are retained within cells and not secreted, so measurement first requires cell lysis. Pyrrolnitrin can then be measured using any analytical chemistry method known in the art, including but not limited to, high performance liquid chromatography with ultraviolet detection (HPLC-UV) of a composition or formulation, such as that described in Hill et al. (1994) Appl Env Micro 60(1) 78-85, which is herein incorporated by reference in its entirety. Microbes such as Bacillus spp. produce a variety of secondary metabolites with pesticidal properties, such as polyketides, peptide antibiotics, bacteriocins, and cyclic lipopeptides. Polyketides include bacillaene, difficidin, macrolactin, aurantinins, and basiliskamide. Cylic lipopeptides include those in the surfactin, iturin, fengycin, and kurstakin families. Biosurfactants such as lipopeptides are also produced by Pseudomonas spp., such as for example tensin, pseudophomin, massetolid, pseudodesmin, xantholysin, and syringomycin families. The presence and/or concentration of a secondary metabolite such as a cyclic lipopeptide or polyketide many be used as a surrogate of the viability and/or pesticidal activity of the bacterial strain in the composition or formulation. For example, lipopeptides may be extracted from a bacterial culture using methods well known in the art, such as a combination of acid precipitation and solvent extraction. The lipopeptide may then be purified and measured by a variety of methods, including membrane ultrafiltration, ionic exchange chromatography, adsorption-desorption on resins, HPLC-UV, hydrophobic interaction chromatography, and/or gel filtration (Ines and Dhouha (2015) Peptides 71: 100- 112).

In some embodiments, the presently disclosed compositions or formulations comprise between about 100 μg/g to 2000 μg/g, 200 μg/g to 1800 μg/g, 300 μg/g to 1500 μg/g, 300 μg/g to 1300 μg/g, 400 μg/g to 1500 μg/g, 400 μg/g to 1300 μg/g, 300 μg/g to 1000 μg/g, 400 μg/g to 1000 μg/g, 500 μg/g to 1000 μg/g, 500 μg/g to 1300 μg/g, 600 μg/g to 1000 μg/g, 600 μg/g to 1300 μg/g, 600 μg/g to 1500 μg/g, or about 300 μg/g, about 400 μg/g, about 500 μg/g, about 600 μg/g, about 700 μg/g, about 800 μg/g, about 900 μg/g, about 1000 μg/g, about 1100 μg/g, about 1200 μg/g, about 1300 μg/g, about 1400 μg/g, about 1500 μg/g, about 1500 μg/g, about 1600 μg/g, about 1700 μg/g, about 1800 μg/g, about 1900 μg/g, and about 2000 μg/g expressed as pg of of a secondary metabolite per g of bacteria. In further embodiments, the secondary metabolite may be pyrrolnitrin, bacillaene, difficidin, macrolactin, aurantinins, basiliskamide, or a member of the tensin, pseudophomin, massetolid, pseudodesmin, xantholysin, syringomycin, surfactin, iturin, fengycin, or kurstakin families.

Further provided is a composition comprising a whole cell broth, supernatant, filtrate, or extract derived from bacterial strain AIP015329, AIP075655, or AIP059286 or an active variant of any thereof, wherein an effective amount of the composition improves an agronomic trait of interest of a plant or controls a plant pest or a plant pathogen that causes disease. The composition contains effective compound(s), metabolite(s), and/or protein(s) which improve an agronomic trait of interest of a plant or controls a plant pest or a plant pathogen that causes disease. The supernatant refers to the liquid remaining when cells are grown in broth or are harvested in another liquid from an agar plate and are removed by centrifugation, filtration, sedimentation, or other means well known in the art. The supernatant may be further concentrated to produce a filtrate. The filtrate may comprise a concentrated amount of an effective compound or metabolite compared to the concentration of the effective compound or metabolite in the supernatant or whole cell broth. In some embodiments, the supernatant, filtrate, or extract may be processed to a wettable powder, spray dried formulation, and/or seed coating. In other embodiments, the supernatant, filtrate, or extract may be concentrated (e.g., water is removed) but remain in a liquid formulation. The composition described above can be applied alone or in combination with another substance, in an effective amount to control a plant pest or improve an agronomic trait of interest of a plant. The various formulations disclosed herein can be stable for at least 30, 40, 50, 60, 70, 80, 90, 100, 125, 150, 200, 225, 250, 275, 300, 325, 350 days, 1.5 years, 2 years or longer. By “stable formulation” it is intended that the formulation retains viable bacteria and/or retains an effective amount of a biologically active bacterial population. The bacterial population may comprise bacterial cells, spores, forespores, or a combination of any of these. Biological activity as used herein refers to the ability of the formulation to improve an agronomic trait of interest or control a plant pest or a plant pathogen that causes a plant disease. In one embodiment, the stable formulation retains at least about 1%, about 10%, about 20%, about 30% about 40%, about 50%, about 60%, about 70%, about 80%, or about 90% of CFU/gram in the formulation at a given storage time point when compared to the CFU/gram produced after immediate preparation of the formulation. In another embodiment, the stable formulation retains at least about 30% to 80%, about 50% to about 80%, about 60% to about 70%, about 70% to about 80%, about 40% to about 50%, about 50% to about 60%, about 60% to about 70% of biological activity (e.g., antifungal activity as calculated using a reporter metabolite such as pyrrolnitrin) in the formulation at a given storage time point when compared to the biological activity found in the formulation immediately after production. In another embodiment, the stable formulation at a given storage time point retains at least about 30%, 45%, 50%, 60%, 70%, 80%, 90% of biological activity when compared to the biological activity found in the formulation immediately after production. In still another embodiment, the stable formation retains any combination of the viability and biological activity noted above.

The formulations preferably comprise between 0.00000001 % and 98% by weight of active compound or, with particular preference, between 0.01 % and 95% by weight of active compound, more preferably between 0.5% and 90% by weight of active compound, based on the weight of the formulation.

The active compound content of the application forms prepared from the formulations may vary within wide ranges. The active compound concentration of the application forms may be situated typically between 0.00000001 % and 95% by weight of active compound, preferably between 0.00001 % and 1 % by weight, based on the weight of the application form. Application takes place in a customary manner adapted to the application forms.

Moreover, the bacterial strain AIP015329, AIP075655, or AIP059286 provided herein or an active variant of any thereof can be mixed with a biocide, such as a fungicide, insecticide, or herbicide to enhance its activity or the activity of the chemical to which it has been added.

In specific embodiments, the bacterial strain AIP015329, AIP075655, or AIP059286 or active variant of any thereof and/or a composition derived therefrom is compatible with agricultural chemicals used to improve performance of biocides. Such agricultural chemicals include safeners, surfactants, stickers, spreaders, UV protectants, and suspension and dispersal aids. Safeners are chemicals that improve or modify the performance of herbicides. Surfactants, spreaders, and stickers are chemicals included in agricultural spray preparations that change the mechanical properties of the spray (for example, by altering surface tension or improving leaf cuticle penetration). UV protectants improve the performance of agricultural biocides by reducing degradation by ultraviolet light. Suspension and dispersal aids improve the performance of biocides by altering their behavior in a spray tank. In instances where the bacterial strain is not compatible with an agricultural chemical of interest, if desired, methods can be undertaken to modify the bacterial strain to impart the compatibility of interest. Such methods to produce modified bacterial strains include both selection techniques and/or transformation techniques.

The AIPO 15329, AIP075655, or AIP059286 bacterial strain provided herein, an active variant of any thereof, and/or a composition derived therefrom can be used to significantly improve at least one agronomic trait of interest (i.e, reduce disease caused by a plant pathogen (e.g., fungal pathogen or fungal-like pathogen) or reduce susceptibility to plant pests). The bacterial strain provided herein, an active variant thereof, and/or a composition derived therefrom can be used with other pesticides for an effective integrated pest management program. In one embodiment, the biocontrol populations can be mixed with known pesticides in a manner described in WO 94/10845, herein incorporated by reference.

Non-limiting examples of compounds and compositions that can be added to the formulation, include but are not limited to, Acetyl tributyl citrate [Citric acid, 2-(acetyloxy)-, tributyl ester]; Agar;

Almond hulls; Almond shells; alpha-Cyclodextrin; Aluminatesilicate; Aluminum magnesium silicate [Silicic acid, aluminum magnesium salt]; Aluminum potassium sodium silicate [Silicic acid, aluminum potassium sodium salt]; Aluminum silicate; Aluminum sodium silicate [Silicic acid, aluminum sodium salt]; Aluminum sodium silicate (1:1: l)[Silicic acid (H4Si04), aluminum sodium salt (1:1:1)]; Ammonium benzoate [Benzoic acid, ammonium salt]; Ammonium stearate [Octadecanoic acid, ammonium salt]; Amylopectin, acid-hydrolyzed, 1-octenylbutanedioate; Amylopectin, hydrogen 1-octadecenylbutanedioate; Animal glue; Ascorbyl palmitate; Attapulgite-type clay; Beeswax; Bentonite; Bentonite, sodian; beta-Cyclodextrin; Bone meal; Bran; Bread crumbs; (+)-Butyl lactate; [Lactic acid, n-butyl ester, (S)]; Butyl lactate [Lactic acid, n- butyl ester]; Butyl stearate [Octadecanoic acid, butyl ester]; Calcareous shale; Calcite (Ca(Co3)); Calcium acetate; Calcium acetate monohydrate [Acetic acid, calcium salt, monohydrate]; Calcium benzoate [Benzoic acid, calcium salt]; Calcium carbonate; Calcium citrate [Citric acid, calcium salt]; Calcium octanoate; Calcium oxide silicate (Ca30(SiOz _t )); Calcium silicate [Silicic acid, calcium salt]; Calcium stearate [Octadecanoic acid, calcium salt]; Calcium sulfate; Calcium sulfate dehydrate; Calcium sulfate hemihydrate; Canary seed; Carbon; Carbon dioxide; Carboxymethyl cellulose [Cellulose, carboxymethyl ether]; Cardboard; Camauba wax; Carob gum [Locust bean gum]; Carrageenan; Caseins; Castor oil; Castor oil, hydrogenated; Cat food; Cellulose; Cellulose acetate; Cellulose, mixture with cellulose carboxymethyl ether, sodium salt; Cellulose, pulp; Cellulose, regenerated; Cheese; Chlorophyll a; Chlorophyll b; Citrus meal; Citric acid; Citric acid, monohydrate; Citrus pectin; Citrus pulp; Clam shells; Cocoa; Cocoa shell flour; Cocoa shells; Cod-liver oil; Coffee grounds; Cookies; Cork; Com cobs; Cotton; Cottonseed meal; Cracked wheat; Decanoic acid, monoester with 1,2,3-propanetriol; Dextrins; Diglyceryl monooleate [9- Octadecenoic acid, ester with 1,2,3-propanetriol]; Diglyceryl monostearate [9-Octadecanoic acid, monoester with xybis(propanediol)]; Dilaurin [Dodecanoic acid, diester with 1,2,3-propanetriol]; _.. Dipalmitin [Hexadecanoic acid, diester with 1,2,3-propanetriol]; Dipotassium citrate [Citric acid, dipotassium salt]; Disodium citrate [Citric acid, disodium salt]; Disodium sulfate decahydrate ; Diatomaceous earth (less than 1% crystalline silica); Dodecanoic acid, monoester with 1,2,3-propanetriol; Dolomite; Douglas fir bark; Egg shells; Eggs; (+)-Ethyl lactate [Lactic acid, ethyl ester, (S)]; Ethyl lactate [Lactic acid, ethyl ester]; Feldspar; Fish meal; Fish oil (not conforming to 40 CFR 180.950) ; Fuller's earth; Fumaric acid; gamma-Cyclodextrin; Gelatins; Gellan gum; Glue (as depolymd. animal collagen); Glycerin [1,2,3-Propanetriol]; Glycerol monooleate [9-Octadecenoic acid (Z)-, 2,3-dihydroxypropyl ester]; Glyceryl dicaprylate [Octanoic acid, diester with 1,2,3-propanetriol]; Glyceryl dimyristate [Tetradecanoic acid, diester with 1,2,3-propanetriol]; Glyceryl dioleate [9-Octadecenoic acid (9Z)-, diester with 1,2,3-propanetriol]; Glyceryl distearate ; Glyceryl monomyristate [Tetradecanoic acid, monoester with 1,2,3-propanetriol]; Glyceryl monooctanoate [Octanoic acid, monoester with 1,2,3-propanetriol]; Glyceryl monooleate [9-Octadecenoic acid (9Z)-, monoester with 1,2,3-propanetriol]; Glyceryl monostearate [Octadecanoic acid, monoester with 1,2,3-propanetriol];

Glyceryl stearate [Octadecanoic acid, ester with 1,2,3-propanetriol]; Granite; Graphite; Guar gum; Gum Arabic; Gum tragacanth; Gypsum; Hematite (Fe203); Humic acid; Hydrogenated cottonseed oil; Hydrogenated rapeseed oil; Hydrogenated soybean oil; Hydroxyethyl cellulose [Cellulose, 2-hydroxyethyl ether]; Hydroxypropyl cellulose [Cellulose, 2-hydroxypropyl ether]; Hydroxypropyl methyl cellulose [Cellulose, 2-hydroxypropyl methyl ether]; Iron magnesium oxide (Fe ₂ MgO ₄ ); Iron oxide (Fe ₂ O ₃ ); Iron oxide (Fe ₂ O ₃ ); Iron oxide (Fe ₃ O4); Iron oxide (FeO); Isopropyl alcohol [2 -Propanol]; Isopropyl myristate; Kaolin; Lactose; Lactose monohydrate; Lanolin; Latex rubber; Laurie acid; Lecithins; Licorice extract; Lime (chemical) dolomitic; Limestone; Linseed oil; Magnesium carbonate [Carbonic acid, magnesium salt (1:1); Magnesium benzoate; Magnesium oxide; Magnesium oxide silicate (Mg ₃ O(Si ₂ O ₅ ). monohydrate; Magnesium silicate; Magnesium silicate hydrate; Magnesium silicon oxide (Mg ₂ Si ₃ O ₈ ): Magnesium stearate [Octadecanoic acid, magnesium salt]; Magnesium sulfate; Magnesium sulfate heptahydrate; Malic acid;

Malt extract; Malt flavor; Maltodextrin; Methylcellulose [Cellulose, methyl ether]; Mica; Mica-group minerals; Milk; N/A Millet seed; Mineral oil (U.S.P.); 1-Monolaurin [Dodecanoic acid, 2,3-dihydroxypropyl ester]; 1-Monomyristin [Tetradecanoic acid, 2,3-dihydroxypropyl ester]; Monomyristin [Decanoic acid, diester with 1,2,3-propanetriol]; Monopalmitin [Hexadecanoic acid, monoester with 1,2,3-propanetriol]; Monopotassium citrate [Citric acid, monopotassium salt; Monosodium citrate [Citric acid, monosodium salt]; Montmorillonite; Myristic acid; Nepheline syenite; Nitrogen; Nutria meat; Nylon; Octanoic acid, potassium salt; Octanoic acid, sodium salt; Oils, almond; Oils, wheat; Oleic acid; Oyster shells; Palm oil; Palm oil, hydrogenated; Palmitic acid [Hexadecanoic acid]; Paraffin wax; Peanut butter; Peanut shells ; Peanuts; Peat moss; Pectin; Perlite; Perlite, expanded; Plaster of paris; Polyethylene; Polyglyceryl oleate; Polyglyceryl stearate; Potassium acetate [Acetic acid, potassium salt]; Potassium aluminum silicate, anhydrous; Potassium benzoate [Benzoic acid, potassium salt]; Potassium bicarbonate [Carbonic acid, monopotassium salt]; Potassium chloride; Potassium citrate [Citric acid, potassium salt]; Potassium humate [Humic acids, potassium salts]; Potassium myristate [Tetradecanoic acid, potassium salt]; Potassium oleate [9-Octadecenoic acid (9Z)-, potassium salt; Potassium ricinoleate [9-Octadecenoic acid, 12-hydroxy-, monopotassium salt,(9Z,12R)-]; Potassium sorbate [Sorbic acid, potassium salt ]; Potassium stearate [Octadecanoic acid, potassium salt]; Potassium sulfate; Potassium sulfate [Sulfuric acid, monopotassium salt]; 1,2-Propylene carbonate [l,3-Dioxolan-2-one, 4-methyl-]; Pumice; Red cabbage color (expressed from edible red cabbage heads via a pressing process using only acidified water); Red cedar chips; Red dog flour; Rubber; Sawdust; Shale; Silica, amorphous, fumed (crystalline free); Silica, amorphous, precipated and gel; Silica (crystalline free); Silica gel; Silica gel, precipitated, crystalline-free; Silica, hydrate; Silica, vitreous; Silicic acid (H2S1O3), magnesium salt (1:1); Soap (The water soluble sodium or potassium salts of fatty acids produced by either the saponification of fats and oils, or the neutralization of fatty acid); Soapbark [Quillaja saponin]; Soapstone; Sodium acetate [Acetic acid, sodium salt]; Sodium alginate; Sodium benzoate [Benzoic acid, sodium salt]; Sodium bicarbonate; Sodium carboxymethyl cellulose [Cellulose, carboxymethyl ether, sodium salt]; Sodium chloride; Sodium citrate; Sodium humate [Humic acids, sodium salts]; Sodium oleate; Sodium ricinoleate [9-Octadecenoic acid, 12-hydroxy-, monosodium salt, (9Z,12R)-]; Sodium stearate [Octadecanoic acid, sodium salt]; Sodium sulfate; Sorbitol [D-glucitol]; Soy protein; Soya lecithins [Lecithins, soya]; Soybean hulls; Soybean meal; Soybean, flour; Stearic acid [Octadecanoic acid]; Sulfur; Syrups, hydrolyzed starch, hydrogenated; Tetragylceryl monooleate [9-Octadecenoic acid (9Z)-, monoester with tetraglycerol]; Tricalcium citrate [Citric acid, calcium salt (2:3)]; Triethyl citrate [Citric acid, triethyl ester; Tripotassium citrate [Citric acid, tripotassium salt]; Tripotassium citrate monohydrate [Citric acid, tripotassium salt, monohydrate]; Trisodium citrate [Citric acid, trisodium salt]; Trisodium citrate dehydrate [Citric acid, trisodium salt, dehydrate]; Trisodium citrate pentahydrate [Citric acid, trisodium salt, pentahydrate]; Ultramarine blue [C.I. Pigment Blue 29]; Urea; Vanillia; Vermiculite; Vinegar (maximum 8% acetic acid in solution); Vitamin C [L-Ascorbic acid]; Vitamin; Walnut flour; Walnut shells; Wheat; Wheat flour; Wheat germ oil; Whey; White mineral oil (petroleum); Wintergreen oil; Wollastonite (Ca(Si03)); Wool; Xanthan gum; Yeast; Zeolites (excluding erionite (CAS Reg. No. 66733-21-9)); Zeolites, NaA; Zinc iron oxide; Zinc oxide (ZnO); and Zinc stearate [Octadecanoic acid, zinc salt].

IV. Methods of Use

The bacterial strain AIP015329, AIP075655, or AIP059286, modified bacterial strains active variants of any thereof, and/or compositions derived therefrom provided herein can be employed with any plant species to improve an agronomic trait of interest. Agonomic traits of interest include any trait that improves plant health or commercial value. Non-limiting examples of agronomic traits of interest including increase in biomass, increase in drought tolerance, thermal tolerance, herbicide tolerance, drought resistance, nematode resistance, insect resistance, fungus resistance, virus resistance, bacteria resistance, male sterility, cold tolerance, salt tolerance, increased yield, enhanced nutrient use efficiency, increased nitrogen use efficiency, increased tolerance to nitrogen stress, increased fermentable carbohydrate content, reduced lignin content, increased antioxidant content, enhanced water use efficiency, increased vigor, increased germination efficiency, earlier or increased flowering, increased biomass, altered root-to-shoot biomass ratio, enhanced soil water retention, or a combination thereof. In other instance, the agronomic trait of interest includes an altered oil content, altered protein content, altered seed carbohydrate composition, altered seed oil composition, altered seed protein composition, chemical tolerance, cold tolerance, delayed senescence, disease resistance, drought tolerance, ear weight, growth improvement, health enhancement, heat tolerance, herbicide tolerance, herbivore resistance, improved nitrogen fixation, improved nitrogen utilization, improved root architecture, improved water use efficiency, increased biomass, increased root length, increased seed weight, increased shoot length, increased yield, increased yield under water-limited conditions, kernel mass, kernel moisture content, metal tolerance, number of ears, number of kernels per ear, number of pods, nutrition enhancement, pathogen resistance, pest resistance, photosynthetic capability improvement, salinity tolerance, stay-green, vigor improvement, increased dry weight of mature seeds, increased fresh weight of mature seeds, increased number of mature seeds per plant, increased chlorophyll content, increased number of pods per plant, increased length of pods per plant, reduced number of wilted leaves per plant, reduced number of severely wilted leaves per plant, and increased number of non-wilted leaves per plant, a detectable modulation in the level of a metabolite, a detectable modulation in the level of a transcript, or a detectable modulation in the proteome relative to a reference plant.

In one non-limiting embodiment, the bacterial strain AIP015329, AIP075655, or AIP059286, an active variant of any thereof, and/or a composition derived therefrom provided herein can be employed with any plant species susceptible to a plant disease. By “a plant susceptible to a plant disease” is meant that the causative pathogen(s) of the plant disease are able to infect the plant. For example, a plant susceptible to a plant disease can be susceptible to a plant disease caused by a fungi or fungal-like organism (e.g., an Oomycete such as Phytophthora or Pythium) as disclosed elsewhere herein.

As used herein, the term plant includes plant cells, plant protoplasts, plant cell tissue cultures from which plants can be regenerated, plant calli, plant clumps, and plant cells that are intact in plants or plant parts such as embryos, pollen, ovules, seeds, leaves, flowers, branches, fruit, kernels, ears, cobs, husks, stalks, roots, root tips, anthers, and the like. Grain is intended to mean the mature seed produced by commercial growers for purposes other than growing or reproducing the species.

Plants of interest include monocotyledonous plants, also referred to as monocots, and dicotyledonous plants, also referred to as dicots. Examples of plant species of interest include, but are not limited to, species, cultivars, varieties, and hybrids of com (Zea mays), Brassica sp. (e.g., B. napus, B. rapa, B. juncea), particularly those Brassica species useful as sources of seed oil, alfalfa ( Medicago sativa), rice ( Oryza sativa), rye ( Secale cereale), sorghum (Sorghum bicolor, Sorghum vulgare), millet (e.g., pearl millet ( Pennisetum glaucum), proso millet ( Panicum miliaceum), foxtail millet (Setaria italica), finger millet ( Eleusine coracana)), sunflower ( Helianthus annuus), safflower ( Carthamus tinctorius), wheat ( Triticum aestivum), soybean ( Glycine max), tobacco ( Nicotiana tabacum), potato ( Solarium tuberosum), peanuts ( Arachis hypogaea), cotton ( Gossypium barbadense, Gossypium hirsutum), sweet potato ( Ipomoea batatus), cassava ( Manihot esculenta), coffee ( Coffea spp.), coconut ( Cocos nucifera), pineapple ( Ananas comosus), citrus trees ( Citrus spp.), cocoa ( Theobroma cacao), tea ( Camellia sinensis), banana ( Musa spp.), avocado (Persea americana), fig {Ficus casica), guava ( Psidium guajava), mango ( Mangifera indica), olive (Olea europaea), papaya ( Carica papaya), grape ( Vitus spp.), strawberry ( Fragaria x ananassa), cherry ( Prunus spp.), apple ( Malus domestica), orange ( Citrus x .s7/7t _' m7.v) ₌ cashc\Y ( Anacardium occidentale), macadamia ( Macadamia integrifolia), almond ( Prunus amygdalus), sugar beets (Beta vulgaris), sugarcane ( Saccharum spp.), Cannabis (including Cannabis sativa, Cannabis indica, and Cannabis ruderalis), oats, barley, vegetables, ornamentals, and conifers.

Plants of interest also include root and tuber vegetables including species, cultivars, varieties, and hybrids of carrot, potato, radish, sweet potato, yam, turnip, rutabaga, arracacha, arrowroot, Chinese artichoke, Jerusalem artichoke, garden beet, burdock, canna, cassava, celeriac, chervil, chicory, chufa, taro/dasheen, ginger, ginseng, horseradish, leren, parsnip, rutabaga, turnip, tanier and sugarbeet; bulb vegetables including species, cultivars, varieties, and hybrids of onion, green onion, leek, and shallot; leafy vegetables such as head lettuce, leaf lettuce, spinach, celery, Swiss chard, watercress, rhubarb, kale, bok choy, endive, collards, cilantro, dandelion, and mustard greens; Brassica head and stem vegetables including species, cultivars, varieties, and hybrids of broccoli, broccoli raab (rapini), gai Ion, gai choy, cabbage, Chinese cabbage, Brussels sprouts, cauliflower, kohlrabi, mizuna, mustard greens, and rape greens; legume vegetables including species, cultivars, varieties, and hybrids of bean or pea, including Lupinus spp. including grain lupin, sweet lupin, white lupin, Phaeolus spp. (including green bean, kidney bean, lima bean, navy bean, pinto bean, runner bean, snap bean, tepary bean, wax bean), Vigna spp. (including adzuki bean, asparagus bean, blackeyed pea, catjang, Chinese longbean, cowpea, crowder pea, moth bean, mung bean, rice bean, southern pea, urd bean, and yardlong bean), Pisum spp. (including dwarf pea, edible-podded pea, English pea, field pea, garden pea, green pea, snowpea, and sugar snap pea), favabean, chickpea, guar, jackbean, lentil,; pigeon pea, soybean, sword bean,, soybean, succulent cultivars of edible-podded bean or edible-podded pea, succulent shelled cultivars of bean or pea, and dried cultivars of bean or pea; fruiting vegetables including species, cultivars, varieties, and hybrids of tomato, bell peper, non-bell pepper, pimento pepper, chili pepper, eggplant, bush tomato, currant tomato, garden huckleberry, goji berry, ground cherry, okra, tomatillo, sunberry, pepino, African eggplant, scarlet eggplant, pea eggplant, martynia, and roselle; cucurbit vegetables including species, cultivars, varieties, and hybrids of cucumber, muskmelon including hybrids and/or varieties of Cucumis spp. such as cantaloupe, watermelon, and honeydew, squash including pumpkin and winter squash including butternut, calabaza, and acorn squash, and summer squash including Cucurbitaceae family members such as hybrids and/or varieties of Cucurbita pepo such as crookneck squash and straightneck squash, hybrids and/or varieties of Lagenaria spp. such as spaghetti squash, hyotan, and cucuzza, Luffa spp. such as hechima and Chinese okra, Momordica spp. such as bitter melon, balsam pear, and Chinese cucumber, and Sechium edule (chayote);Citrus including species, cultivars, varieties, and hybrids of orange, tangerine, mandarin, lemon, lime, grapefruit, pummelo, tangor, uniq fruit, and kumquat; pome fruit including species, cultivars, varieties, and hybrids of apple, pear, quince, Chinese quince, tejocote, mayhaw, loquat, and azarole; stone fruit including species, cultivars, varieties, and hybrids of sweet cherry, tart cherry, peach, plum, prune plum, apricot, Jujube, nectarine, and plumcot; berry and small fruit including species, cultivars, varieties, and hybrids of blackberry, raspberry, highbush blueberry, caneberry, bushberry, large shrub or tree berry including elderberry, and mulberry, small fruit climbing vine including grape, gooseberry, Amur river grape, may pop, fuzzy kiwifiuit, and hardy kiwifiuit, low growing berry including strawberry, bearberry, lingonberry, cranberry, cloudberry, muntries, and partridgeberry; tree nut including species, cultivars, varieties, and hybrids of almond, pecan, Brazil nut, butternut, cashew, chestnut, ginkgo, Okari nut, pine nut, pistachio, walnut, and bunya; cereal grain including species, cultivars, varieties, and hybrids of com (sweet com and field com), rice, sorghum, amaranth, lupine, wheat, millet, barely, buckwheat, oats, rye, and wild rice; grass forage, fodder and hay including species, cultivars, varieties, and hybrids of Bermuda grass, bluegrass, bentgrass, bromegrass, Sudan grass, switch grass, Poa annua, and fescue; nongrass animal feeds including species, cultivars, varieties, and hybrids of alfalfa, Trifolium spp. and Melilotus spp.; oil seed including species, cultivars, varieties, and hybrids of rapeseed, sunflower seed, cottonseed, canola, calendula, castor oil plant, safflower, poppyseed, sesame, flax, milkweed, tea oil plant, Brassica napus, Brassica campestris, and Crambe abyssinica; stalk, stem, and leaf petiole vegetable including species, cultivars, varieties, and hybrids of asparagus, agave, artichoke, aloe vera, bamboo, fennel, fiiki, palm hearts, prickly pear, udo, Chinese celery, and celery; tropical and sub-tropical fmit with an edible peel including species, cultivars, varieties, and hybrids of date, fig, guava, olive; tropical and sub-tropical fruit with an inedible peel including species, cultivars, varieties, and hybrids of atemoya, sugar apple, avocado, mango, papaya, banana, plantains, pomegranate, dragon fmit, lychee, passionfmit, pineapple, durian, and prickly pear; herb including species, cultivars, varieties, and hybrids of basil, mint, marigold, geranium, echinacea, Rooibos, sage, savory, thyme, tarragon, violet, yerba santa, yomogi, and wild bergamot; spice including species, cultivars, varieties, and hybrids of dill seed, celery seed, allspice, anise pepper, anise seed, star anise, annatto, balsam, caraway, cardamom, Cassia, nutmeg, cinnamon, clove, coriander, cumin, echinacea, eucalyptus, fennel, fenugreek, mustard, pepper, pepperbush, peppercorn, peppertree, quinine, me, saffron, sandalwood, sassafras, sumac, tamarind, vanilla, willow, witch hazel, and yohimbe. Plants of interest also include species, cultivars, varieties, and hybrids of cotton, linen, tobacco, and hops.

Plants of interest include ornamental plants, flowers, flowering plants, tropical foliage, foliage, trees, shrubs, forestry, and grasses, including lawn, sod, turf, and ornamental turf. Plants of interest include annual and perennial flower plants including species, cultivars, varieties, and hybrids of Alyssum, Chrysantheum,

Easter lily, Hydrangea, Marigols, Ranunculus, Verbena spp., roses ( Rosa spp.), tulips ( Tulipa spp.), daffodils ( Narcissus spp.), Carnation, Asters, Garden Phlox, Impatiens, Orchids, Roses, Vinca, Azalea, Cyclamen, Geraniums, Kalanchoe, Pansies, Salvia spp., Violas, Begonia, Dianthus, Gerbera, Linaria, Petunia, Snapdragons, Zinnias, Calla lily, Dwarf Bee-balm, Golden Star, Lisianthus, Poinsettia, Stock, Lobelia, Portulaca; tropical foliage including species, cultivars, varieties, and hybrids of Aglaonema, Hibiscus, Dieffenbachia, Leatherleaf Fern, Dracaena spp., English Ivy, and Spathiphyllum; trees and shmbs including species, cultivars, varieties, and hybrids of Azalea boxwood, Gumpo azalea, Ligustrum japonicum, Photinia, Spirea, Crape myrtle, Indian hawthorn, Lilac, Rhododendron, Dogwood, Japanese maple, Loropetalum, Soft Touch holly, Rhododendron spp., Rosaceae spp., Pinus spp. (including loblolly pine, slash pine, ponderosa pine, lodgepole pine, and Monterey pine ), Douglas-fir, Western hemlock, Sitka spruce, redwood, tme firs such as silver fir and balsam fir, and cedars such as Western red cedar and Alaska yellow-cedar; lawn and turf including species, cultivars, varieties, and hybrids of bluegrass, bentgrass, Bermudagrass, Dichondra, Fescue, Orchardgrass, Poa annua, St. Augustine, Ryegrass, Zoysia, and mixtures thereof.

In specific embodiments, plants of the present invention are row crop plants (for example, com, alfalfa, sunflower, Brassica spp, Phaaeolus spp., Pisum spp., soybean, cotton, flax, buckwheat, sugarbeets, safflower, peanut, sorghum, sugarcane, wheat, millet, tobacco, etc.). In other embodiments, com and soybean plants are preferred, and in yet other embodiments com plants are preferred. In other embodiments, plants of the present invention are permanent crops (for example, wine grapes, pistachios, walnuts, almonds, coconuts, pecans, apples, pears, avocados, citms, etc.). Other plants of interest include grain plants that provide seeds of interest, oil-seed plants, and leguminous plants. Seeds of interest include grain seeds, such as com, wheat, barley, rice, sorghum, rye, etc. Oil-seed plants include cotton, soybean, safflower, sunflower, Brassica, maize, alfalfa, palm, coconut, etc. Leguminous plants include beans, peas, and dry pulses. Beans include guar, locust bean, fenugreek, soybean, garden beans, cowpea, mungbean, lima bean, fava bean, lentils, chickpea, etc.

A. Non-limiting Plant Pests

In specific embodiments, the bacterial strains provided herein are those that target one or more plant pests. The term “pests” or “plant pests” includes but is not limited to insects, fungi, fungal-like organisms, bacteria, nematodes, vimses, viroids, protozoan pathogens, and the like. Plant pathogens include but are not limited to vimses, viroids, bacteria, insects which vector or spread plant diseases, nematodes, Oomycetes, plasmodiophorids, members of the Phytomyxea, fungi, fungal -like organisms, and the like. In specific embodiments, the bacterial strains, or active variants thereof, provided herein are those that target one or more plant pests. For example, any of the bacterial strain provided herein or active variant thereof can have antifungal activity against one, two, three, four, five, or more fungal pathogens and/or fungal diseases described herein. Examples of plant diseases which can be treated or reduced or prevented include, but are not limited to, plant diseases caused by plant pathogens. Examples of such plant diseases include, but are not limited to, Asian Soybean Rust (ASR), gray mold, leaf spot, Frogeye Leaf Spot, Early Blight, Damping off complex, Brown Patch, black scurf, root rot, belly rot, Sheath Blight, Powdery Mildew, Anthracnose, Black Sigatoka, Anthracnose leaf spot, Downy Mildew, Pythium Blight, Late Blight, Fusarium Head Blight, sudden death syndrome (SDS), Fusarium Wilt, Com Stalk Rot, Brown Rust, Black Rust, Yellow Rust, Wheat Rust, Rust, Apple Scab, Post-bloom Fmit Drop, Gummy Stem Blight, Greasy Spot, Com Stalk Rot, Cherry Blossom Blight, Damping Off, Fire Blight, Citms Greening Disease, Clubroot, Verticillium Wilt, and Brown Rot, to name a few.

The methods and compositions disclosed herein can be used to control one or more fungal or fungal- like pathogens. In further embodiments, the bacterial strains or active variants thereof (i.e., AIP015329, AIP075655, or AIP059286 or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores, and/or a composition derived from any one thereof) control at least one, two, three, four, five, or more fungal pathogens and/or fungal-like pathogens. A fungal pathogen can be, but is not limited to, a fungus selected from the group consisting of Aspergillus flavus, Aspergillus spp., Albugo occidentalis, Albugo spp., Alternaria solani, Alternaria spp., Apiognomonia errabunda, Apiognomonia veneta, Apiognomonia spp., Armillaria mellea, Armillaria spp., Bipolaris maydis, Botrytis cinerea, Botrytis squamosa, Botrytis spp., Botryosphaeria dothidea, Botryosphaeira spp., Blumeriella jaapii, Blumeriella spp., Bremia lactucae, Bremia spp., Cladosporium carpophilum, Cladosporium caryigenum, Cladosporium spp., Colletotrichum acutatum, Colletotrichum graminicola, Colletotrichum cereale, Colletotrichum gloeosporiodes, Colletotrichum sublineolum, Colletotrichum spp, Cochliobolus heterostrophus, Corynespora cassiicola, Corynespora spp., Discula fraxinea, Cercospora sojina, Cercospora beticola, Cercospora spp., Blumeria graminis f. sp. Tritici, Didymella bryoniae, Didymella spp., Elsinoe fawcetti, Elsinoe spp., Erysiphe necator, Erysiphe lager stroemiae, Erysiphe cichoracearum, Erysiphe spp., Eutypa lata, Eutypa spp., Fusarium graminearum, Fusarium solani, Fusarium oxysporum, Fusarium graminicola, Fusarium spp., Golovinomyces cichoracearum, Gibberella zeae, Gibberella spp., Gloeodes pomigena, Gymnosporangium juniperi-virginianae, Hemileia vastatrix, Leveillula taurica, Leveillula spp., Mycosphaerella fijiensis, Mycosphaerella citri, Mycosphaerella pomi, Mycosphaerella spp., Macrophomina spp.. Monosporascits cannonballus, Monosporascus spp.. Monilinia fructicola, Monilinia laxa, Monilinia fructigena, Monilinia spp., Neofabraea spp., Podosphaera xanthii, Podosphaera leucotricha, Podosphaera spp., Phomopsis viticola, Phomopsis spp., Penicillium spp., Phakopsora meibomiae, Phakopsora pachyrizi, Phakopsora spp., Puccinia triticina, Puccinia recondita, Puccinia striiformis, Puccinia graminis, Puccinia spp., Pyrenophora trici-repentis, Rhizoctonia solani, Rhizoctonia spp., Rhizopus spp., Ramularia spp., Tilletia barclayena, Tilletia spp., Uncinula necator, Uncinula spp,. Uromyces betae, Uromyces spp., Phoma spp., Sclerotium rolfsii, Sclerotium spp., Sclerotinia minor, Sclerotinia sclerotiorium, Sclerotinia spp., Schizothyrium pomi, Schizothyrium spp., Septoria glycines, Septoria spp., Sphaerotheca pannosa, Sphaerotheca macularis, Sphaerotheca spp., Sphaceloma spp., Venturia inaequalis, Venturia spp., Verticillium spp., Wilsonomyces carpophilus, and Wilsonomyces spp.

In specific embodiments, fungal-like pathogens or fungal-like organisms refers to any organism that exhibits typical phenotypic characterisitics of fungi, but are not technically classified as fungi. In some embodiments, fungal-like pathogens were previously classified as fungi, but have changed classification. In specific embodiments Oomycetes, plasmodiophorids, and members of the Phytomyxea which are plant parasitic are referred to herein as fungal-like pathogens. Fungal-like pathogens can be, but are not limited to, Pythium cryptoirregulare, Pythium aphanidermatum, Pythium irregulare, Pythium sylvaticum, Pythium myriotylum, Pythium ultimum, Pythium spp., Phytophthora capsici, Phytophthora nicotianae, Phytophthora infestans, Phytophthora tropicalis, Phytophthora sojae, Phytophthora spp., Peronospora belbahrii, Peronospora lamii, Peronospora farinosa, Peronospora spp., Pseudoperonospora cubensis, Pseudoperonospora spp., Bremia spp., Plasmopara viticola, Plasmopara obduscens, Plasmopara spp., Basidiophora spp., Plasmodiophora brassicae, and P las modi ophora spp.

In some embodiments, the fungal or fungal-like pathogen is selected from the group consisting of Aspergillus spp., Botrytis spp., Cercospora spp., Alternaria spp., Didymella spp., Fusarium spp., Erysiphe spp., Colletotrichum spp., Monilinia spp., Mycosphaerella spp., Plasmopara spp., Peronospora spp., Pythium spp., Phytophthora spp., Phomopsis spp., Phakopsora spp., Podosphaera spp., Rhizoctonia spp., Sclerotinia spp., Uncinula spp., Venturia spp., Wilsonomyces spp., and P las modi ophora spp.

In further embodiments, the fungal or fungal-like pathogen is selected from the group consisting of Aspergillus flavus, Botrytis cinerea, Cercospora sojina, Alternaria solani, Colletotrichum acutatum, Colletotrichum cereal, Colletotrichum suhlineolum, Didymella hryoniae, Erysiphe necator, Fusarium graminearum, Fusarium solani, Monilinia fructicola, Monilinia laxa, Monilinia fructigena, Mycosphaerella citri, Mycosphaerella fijiensis, Podosphaera xanthii, Plasmopara viticola, P las modi ophora hrassicae, Peronospora helhahrii, Pythium aphanidermatum, Pythium sylvaticum, Pythium myriotylum, Pythium ultimum, Phytophthora nicotianae, Phytophthora infestans, Phytophthora tropicalis, Phytophthora sojae, Phakopsora pachyrizi, Rhizoctonia solani, Sclerotinia minor, Sclerotinia sclerotiorium, Uncinula necator, and Venturia inaequalis. In some embodiments, the bacterial strains or active variants thereof (i.e., AIP015329, AIP075655, AIP059286, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores, and/or a composition derived from AIP015329, AIP075655, AIP059286, or an active variant of any thereof) control at least one, two, three, four, five, or more fungal or fungal -like pathogens selected from the group consisting of Aspergillus flavus, Botrytis cinerea, Cercospora sojina, Alternaria solani, Colletotrichum acutatum, Colletotrichum cereal, Colletotrichum suhlineolum, Didymella hryoniae, Erysiphe necator, Fusarium graminearum, Fusarium solani, Monilinia fructicola, Monilinia laxa, Monilinia fructigena, Mycosphaerella citri, Mycosphaerella fijiensis, Podosphaera xanthii, Plasmopara viticola, Plasmodiophora hrassicae, Peronospora helhahrii, Pythium aphanidermatum,

Pythium sylvaticum, Pythium myriotylum, Pythium ultimum, Phytophthora nicotianae, Phytophthora infestans, Phytophthora tropicalis, Phytophthora sojae, Phakopsora pachyrizi, Rhizoctonia solani, Sclerotinia minor, Sclerotinia sclerotiorium, Uncinula necator, and Venturia inaequalis

In some embodiments, the bacterial strains provided herein are those that control one or more bacterial pathogens. In further embodiments, the bacterial strains or active variants thereof (i.e., AIPO 15329, AIP075655, AIP059286, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores, and/or a composition derived from any one thereof) control at least one, two, three, four, five, or more bacterial pathogens. Bacterial pathogens include Actinobacteria and Proteobacteria and are selected from the families of the Burkholderiaceae, Xanthomonadaceae, Pseudomonadaceae, Enterobacteriaceae, Microbacteriaceae, and Rhizobiaceae. A bacterial pathogen can be, but is not limited to, a bacterial species, sub-species, pathovar, or strain selected from the group consisting of Agrobacterium spp., including Agrobacterium rhizogenes and Agrobacterium tumefaciens; Acidovorax avenae ( =Pseudomonas avenae, Pseudomonas avenae subsp. avenae, Pseudomonas rubrilineans), including Acidovorax avenae subsp. avenae ( =Pseudomonas avenae subsp. avenae), Acidovorax avenae subsp. cattleyae ( =Pseudomonas cattleyae), Acidovorax avenae subsp. citrulli (= Pseudomonas pseudoalcaligene subsp. citrulli, Pseudomonas avenae subsp. citrulli)),' Brenneria spp., Burkholderia spp., including Burkholderia andropogonis ( =Pseudomonas andropogonis, Pseudomonas woodsii), Burkholderia caryophylli ( =Pseudomonas caryophylli), Burkholderia cepacia (= Pseudomonas cepacia), Burkholderia gladioli ( =Pseudomonas gladioli), Burkholderia gladioli pv. agaricicola ( =Pseudomonas gladioli pv. agaricicola), Burkholderia gladioli pv. alliicola ( =Pseudomonas gladioli pv. alliicold), Burkholderia gladioli pv. gladioli ( =Pseudomonas gladioli, Pseudomonas gladioli pv. gladioli), Burkholderia glumae ( =Pseudomonas glumae), Burkholderia plantarii ( =Pseudomonas plantarii) Burkholderia solanacearum ( =Ralstonia solanacearum), and Ralstonia spp.; Liherihacter spp., including Candidatus Liherihacter spp., including Liherihacter africanus (Laf), Liherihacter americanus (Lam), Liherihacter asiaticus (Las), Liherihacter europaeus (Leu), Liherihacter psyllaurous, Liherihacter solanacearum (Lso); Clavihacter spp. including Clavihacter michiganensis and Clavibacter sepedonicus; Corynebacterium, including Corynebacterium fascians, Corynebacterium flaccumfaciens pv . flaccumfaciens, Corynebacterium michiganensis, Corynebacterium michiganense pv. tritici, Corynebacterium michiganense pv. nebraskense, Corynebacterium sepedonicum; Dickeya spp. including Dickeya dadantii and Dickeya solani; Erwinia spp. including Erwinia amylovora, Erwinia ananas, Erwinia carotovora ( =Pectohacterium carotovorum), Erwinia carotovora subsp. atroseptica, Erwinia carotovora subsp. carotovora, Erwinia chrysanthemi, Erwinia chrysanthemi pv. zeae, Erwinia dissolvens, Erwinia herbicola, Erwinia rhapontic, Erwinia stewartiii, Erwinia tracheiphila, Erwinia uredovora; Pseudomonas savastanoi, Pseudomonas syringae, including Pseudomonas syringae pv. actinidiae (Psa), Pseudomonas syringae pv. atrofaciens, Pseudomonas syringae pv. coronafaciens, Pseudomonas syringae pv. glycinea, Pseudomonas syringae pv. lachrymans, Pseudomonas syringae pv. maculicola Pseudomonas syringae pv. papulans, Pseudomonas syringae pv. striafaciens, Pseudomonas syringae pv. syringae, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tabaci; Pectobacterium spp., including Pectobacterium atrosepticum; Streptomyces spp. including Streptomyces acidiscabies, Streptomyces albidoflavus, Streptomyces candidus ( =Actinomyces candidus), Streptomyces caviscabies, Streptomyces collinus, Streptomyces europaeiscahiei, Streptomyces intermedius, Streptomyces ipomoeae, Streptomyces luridiscahiei, Streptomyces niveiscahiei, Streptomyces puniciscahiei, Streptomyces retuculiscahiei, Streptomyces scabiei, Streptomyces scabies, Streptomyces setonii, Streptomyces stelii scabiei, Streptomyces turgidiscabies, Streptomyces wedmorensis; Xanthomonas axonopodis, including Xanthomonas axonopodis pv. alfalfae ( =Xanthomonas alfalfae), Xanthomonas axonopodis pv. aurantifolii ( =Xanthomonas fuscans subsp. aurantifolii), Xanthomonas axonopodis pv. allii ( =Xanthomonas campestris pv. allii), Xanthomonas axonopodis pv. axonopodis, Xanthomonas axonopodis pv. hauhiniae ( =Xanthomonas campestris pv. hauhiniae), Xanthomonas axonopodis pv. begonia ( =Xanthomonas campestris pv. hegoniae), Xanthomonas axonopodis pv. hetlicola ( =Xanthomonas campestris pv. hetlicola), Xanthomonas axonopodis pv. hiophyti ( =Xanthomonas campestris pv. biophyti), Xanthomonas axonopodis pv. cajani ( =Xanthomonas campestris pv. cajani), Xanthomonas axonopodis pv. cassava ( =Xanthomonas cassavae, Xanthomonas campestris pv. cassavae), Xanthomonas axonopodis pv. cassiae ( =Xanthomonas campestris pv. cassiae), Xanthomonas axonopodis pv. citri ( =Xanthomonas citri), Xanthomonas axonopodis pv. citrumelo ( =Xanthomonas alfalfa subsp. citrumelonis), Xanthomonas axonopodis pv. clitoriae ( =Xanthomonas campestris pv. clitoriae), Xanthomonas axonopodis pv. coracanae ( =Xanthomonas campestris pv. coracanae), Xanthomonas axonopodis pv. cyamopsidis ( =Xanthomonas campestris pv. cyamopsidis), Xanthomonas axonopodis pv. desmodii ( =Xanthomonas campestris pv. desmodii), Xanthomonas axonopodis pv. desmodiigangetici ( =Xanthomonas campestris pv. desmodiigangetici), Xanthomonas axonopodis pv. desmodiilaxiflori ( =Xanthomonas campestris pv. desmodiilaxiflori), Xanthomonas axonopodis pv. desmodiirotundifolii ( =Xanthomonas campestris pv. desmodiirotundifolii), Xanthomonas axonopodis pv. dieffenhachiae ( =Xanthomonas campestris pv. dieffenhachiae), Xanthomonas axonopodis pv. erythrinae ( =Xanthomonas campestris pv. erythrinae), Xanthomonas axonopodis pv . fascicularis ( =Xanthomonas campestris pv . fasciculari), Xanthomonas axonopodis pv. glycines ( =Xanthomonas campestris pv. glycines), Xanthomonas axonopodis pv. khayae ( =Xanthomonas campestris pv. khayae), Xanthomonas axonopodis pv. lespedezae ( =Xanthomonas campestris pv. lespedezae), Xanthomonas axonopodis pv. maculifoliigardeniae ( =Xanthomonas campestris pv. maculifoliigardeniae), Xanthomonas axonopodis pv. malvacearum ( =Xanthomonas citri subsp. malvacearum), Xanthomonas axonopodis pv. manihotis ( =Xanthomonas campestris pv. manihotis), Xanthomonas axonopodis pv. martyniicola ( =Xanthomonas campestris pv. martyniicola), Xanthomonas axonopodis pv. melhusii ( =Xanthomonas campestris pv. melhusii), Xanthomonas axonopodis pv. nakataecorchori ( =Xanthomonas campestris pv. nakataecorchori), Xanthomonas axonopodis pv. passiflorae ( =Xanthomonas campestris pv. passiflorae), Xanthomonas axonopodis pv. patelii ( =Xanthomonas campestris pv. patelii), Xanthomonas axonopodis pv. pedalii ( =Xanthomonas campestris pv. pedalii), Xanthomonas axonopodis pv. phaseoli ( =Xanthomonas campestris pv. phaseoli, Xanthomonas phaseoli), Xanthomonas axonopodis pv . phaseoli var.fuscans ( =Xanthomonas fuscans), Xanthomonas axonopodis pv. phyllanthi ( =Xanthomonas campestris pv. phyllanthi), Xanthomonas axonopodis pv. physalidicola ( =Xanthomonas campestris pv. physalidicola), Xanthomonas axonopodis pv. poinsettiicola ( =Xanthomonas campestris pv. poinsettiicola), Xanthomonas axonopodis pv. punicae ( =Xanthomonas campestris pv. punicae), Xanthomonas axonopodis pv. rhynchosiae ( =Xanthomonas campestris pv. rhynchosiae), Xanthomonas axonopodis pv. ricini ( =Xanthomonas campestris pv. ricini), Xanthomonas axonopodis pv. seshaniae ( =Xanthomonas campestris pv. seshaniae), Xanthomonas axonopodis pv. tamarindi ( =Xanthomonas campestris pv. tamarindi), Xanthomonas axonopodis pv. vasculorum ( =Xanthomonas campestris pv. vasculorum), Xanthomonas axonopodis pv. vesicatoria ( =Xanthomonas campestris pv. vesicatoria, Xanthomonas vesicatoria), Xanthomonas axonopodis pv. vignaeradiatae ( =Xanthomonas campestris pv. vignaeradiatae), Xanthomonas axonopodis pv. vignicola ( =Xanthomonas campestris pv. vignicola), Xanthomonas axonopodis pv. vitians ( =Xanthomonas campestris pv. vitians); Xanthomonas campestris pv. musacearum, Xanthomonas campestris pv. pruni ( =Xanthomonas arhoricola pv. pruni), Xanthomonas fragariae; Xanthomonas oryzae, Xanthomonas translucens ( =Xanthomonas campestris pv. hordei) including Xanthomonas translucens pv. arrhenatheri ( =Xanthomonas campestris pv. arrhenatheri), Xanthomonas translucens pv. cerealis ( =Xanthomonas campestris pv. cerealis), Xanthomonas translucens pv. graminis ( =Xanthomonas campestris pv. graminis), Xanthomonas translucens pv. phlei ( =Xanthomonas campestris pv. phlei), Xanthomonas translucens pv. phleipratensis ( =Xanthomonas campestris pv. phleipratensis), Xanthomonas translucens pv. poae ( =Xanthomonas campestris pv. poae), Xanthomonas translucens pv. secalis ( =Xanthomonas campestris pv. secalis), Xanthomonas translucens pv. translucens ( =Xanthomonas campestris pv. translucens), Xanthomonas translucens pv. undulosa ( =Xanthomonas campestris pv. undulosa), Xanthomonas oryzae, Xanthomonas oryzae pv. oryzae ( =Xanthomonas campestris pv. oryzae), Xanthomonas oryzae pv. oryzicola ( =Xanthomonas campestris pv. oryzicola), and Xylella fastidiosa from the family of Xanthomonadaceae .

In some embodiments, the bacterial pathogen is a species, sub-species, pathovar, or strain selected from the group consisting of Acidovorax avenae, Burkholderia gladioli, Candidatus Liherihacter spp., Erwinia amylovora, Erwinia ananas, Erwinia carotovora, Erwinia chrysanthemi, Erwinia dissolvens, Erwinia herhicola, Erwinia rhapontic, Erwinia stewartiii, Erwinia tracheiphila, Erwinia uredovora; Pseudomonas syringae, Streptomyces scabies, Xanthomonas campestris, Xanthomonas axonopodis, Xanthomonas fragariae; Xanthomonas translucens, and Xylella fastidiosa. In some embodiments, the bacterial strains or active variants thereof (i.e., AIPO 15329, AIP075655, AIP059286, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores, and/or a composition derived from AIP015329, or an active variant of thereof) control at least one, two, three, four, five, or more bacterial pathogens selected from the group consisting of a species, sub-species, pathovar, or strain of Acidovorax avenae, Burkholderia gladioli, Candidatus Liberibacter spp., Erwinia amylovora, Erwinia ananas, Erwinia chrysanthemi, Erwinia dissolvens, Erwinia herbicola, Erwinia rhapontic, Erwinia stewartiii, Erwinia tracheiphila, Erwinia uredovora; Pectobacterium carotovorum, Pseudomonas syringae, Streptomyces scabies, Xanthomonas campestris, Xanthomonas axonopodis, Xanthomonas fragariae; Xanthomonas translucens, and Xylella fastidiosa.

In some embodiments, the bacterial strains provided herein are those that control one or more insect or insect pests. In further embodiments, the bacterial strains or active variants thereof (i.e., AIP015329, AIP075655, AIP059286, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores, and/or a composition derived from any one thereof) control at least one, two, three, four, five, or more insects and/or insect pests. The term "insects" or “insect pests” as used herein refers to insects and other similar pests. The insect or insect pest may either feed from a plant tissue, such as a leaf, fruit, stalk, or root, or it may pierce a plant tissue and feed on plant fluids, such as the phloem. The insect or insect pest may act as a vector for plant pathogens, for example for viral or bacterial plant pathogens. The term "insect" encompasses eggs, larvae, juvenile and mature forms of insects. Insects can be targeted at any stage of development. For example, insects can be targeted after the first instar, during the second instar, third instar, fourth instar, fifth instar, or any other developmental or adult growth stage. As used herein, the term “instar” is used to denote the developmental stage of the larval or nymphal forms of insects. Insect pests include insects selected from the orders Acari, Coleoptera, Lepidoptera, Hemiptera, Diptera, Hymenoptera, Lepidoptera, Mallophaga, Homoptera, Hemiptera, Orthroptera, Thysanoptera, Trombidiformes, Dermaptera, Isoptera, Anoplura, Siphonaptera, and Trichoptera.

Insect pests of the order Coleoptera include, but are not limited to, Agriotes spp., Anthonomus spp., Atomaria linearis, Chaetocnema tibialis, Cosmopolites spp., Curculio spp., Dermestes spp., Epilachna spp., Eremnus spp., Leptinotarsa decemlineata, Lissorhoptrus spp.. Meloloniha spp., Orycaephilus spp., Otiorhynchus spp., Phlyctinus spp., Popillia spp., Psylliodes spp., Rhizopertha spp., Scarabeidae, Sitophilus spp., Sitotroga spp., Tenebrio spp., Tribolium spp., and Trogoderma spp. In specific embodiments, Coleoptera insects include, but are not limited to weevils from the families Anthribidae, Bruchidae, and Curculionidae (e.g., sweetpotato weevil ( Cylas formicarius (Fabricius)), boll weevil ( Anthonomus grandis Boheman), rice water weevil ( Lissorhoptrus oryzophilus Kuschel), rice weevil ( Sitophilus oryzae L.)); flea beetles, cucumber beetles, rootworms, leaf beetles, potato beetles, and leafminers in the family Chrysomelidae (e.g., Colorado potato beetle ( Leptinotarsa decemlineata Say), western com rootworm ( Diabrotica virgifera virgifera LeConte)); chafers and other beetles from the family Scaribaeidae (e.g., Japanese beetle (Popillia japonica Newman) and European chafer ( Rhizotrogus majalis Razoumowsky)); wireworms from the family Elateridae and bark beetles from the family Scolytidae.

As disclosed herein, insect pests include Coleoptera pests of the com rootworm complex: Western com rootworm, Diabrotica virgifera virgifera ; northern com rootworm, D. barberi ; Southern com rootworm or spotted cucumber beetle, Diabrotica undecimpunctata howardi; and the Mexican com rootworm, D. virgifera zeae. In specific embodiments, the insect pest is Western com rootworm, Colorado Potato Beetle, and/or sweet potato weevil.

Insect pests that can be controlled with the compositions and methods disclosed herein further include insects of the order Lepidoptera, including Achoroia grisella, Acleris gloverana, Acleris variana, Adoxophyes orana, Agrotis ipsilon, Alabama argillacea, Alsophila pometaria, Amyelois transitella, Anagasta kuehniella, Anarsia lineatella, Anisota senatoria, Antheraea pernyi, Anticarsia gemmatalis, Archips spp., Argyrotaenia spp., Athetis mindara, Bombyx mori, Bucculatrix thurberiella, Cadra cautella, Choristoneura sp., Cochylls hospes, Colias eurytheme, Corcyra cephalonica, Cydia latiferreanus, Cydia pomonella, Datana integerrima, Dendrolimus sibericus, Desmiafeneralis spp. , Diaphania hyalinata, Diaphania nitidalis, Diatraea grandiosella, Diatraea saccharalis, Ennomos subsignaria, Eoreuma loftini, Esphestia elutella, Erannis tilaria, Estigmene acrea, Eulia salubricola, Eupocoellia ambiguella, Eupoecilia ambiguella, Euproctis chrysorrhoea, Euxoa messoria, Galleria mellonella, Grapholita molesta, Harrisina americana, Helicoverpa subflexa, Helicoverpa zea, Eleliothis virescens, Hemileuca oliviae, Homoeosoma electellum, Hyphantia cunea, Keiferia lycopersicella, Lambdina fiscellaria fiscellaria, Lambdina fiscellaria lugubrosa, Leucoma salicis, Lobesia botrana, Loxostege sticticalis, Lymantria dispar, Macalla thyrisalis, Malacosoma spp.. Mamestra bras sicae, Marne stra configurata, Manduca quinquemaculata, Manduca sexta, Maruca testulalis , Melanchra picta, Operophtera brumata, Orgyia spp., Ostrinia nubilalis, Paleacrita vernata, Papilio cresphontes, Pectinophora gossypiella, Phryganidia californica, Phyllonorycter blancardella, Pieris napi, Pieris rapae, Plathypena scabra, Platynota flouendana, Platynota stultana, Platyptilia carduidactyla, Plodia interpunctella, Plutella xylostella, Pontia protodice, Pseudaletia unipuncta, Pseudoplasia includens, Sabulodes aegrotata, Schizura concinna, Sitotroga cerealella, Spilonta ocellana, Spodoptera spp. including Spodoptera frugiperda, Thaurnstopoea pityocampa, Tinsola bisselliella, Trichoplusia hi, Tuta absoluta, Udea rubigalis, Xylomyges curiails, and Yponomeuta padella.

The methods and compositions provided herein can also be used against insect pests of the order Hemiptera including, but not limited to, Lygus spp., including Lygus spp. including Lygus hesperus, Lygus lineolaris, Lygus pratensis, Lygus rugulipennis, and Lygus pabulinus, Calocoris norvegicus, Orthops compestris, Plesiocoris rugicollis, Cyrtopeltis modestus, Cyrtopeltis notatus, Spanagonicus albofasciatus, Diaphnocoris chlorinonis, Labopidicola allii, Pseudatomoscelis seriatus, Adelphocoris rapidus, Poecilocapsus lineatus, Blissus leucopterus, Nysius spp. including Nysius ericae and Nysius raphanus, Nezara viridula, Acrosternum hilare, Euschistus spp. including Euschistus servus and Euschistus heros, Dichelops spp. including Dichelops melacantus and Dichelops furcatus, Halyomorpha halys, Lipaphis erysimi, Aphis gossypii, Macrosiphum avenae, Myzus persicae, Acyrthosiphon pisum, Aphidoidea spp, Eurygaster spp., Coreidae spp., Pyrrhocoridae spp., Blostomatidae spp., Reduviidae spp., Cimicidae spp., Aleurocanthus woglumi, Aleyrodes proletella, Bemisia spp. including Bemisia argentifolii and Bemisia tabaci, Trialeurodes vaporariorum, and psyllids including Diaphorina spp. including Diaphorina citri and Trioza spp. including Trioza erytreae.

The methods and compositions provided herein can also be used against insect pests of the order Thysenoptera including, but not limited to, thrips species, including Frankliniella spp., for example Western Flower thrips ( Frankliniella occidentalis (Pergande)); Thrips spp., for example Thrips tabaci; Scirtothrips spp., for example Scirtothrips dorsalis; Klambothrips spp., for example Klambothrips myopori;

Echinothrips spp., for example Echinothrips americanus; and Megalurothrips spp., for example Megalurothrips usitatus.

The methods and compositions provided herein can also be used against insect pests of the order Trombidiformes including, but are not limited to, plant feeding mites, including six-spooted spider mite ( Eutetranychus sexmaculatus), Texas citrus mite ( Eutetranychus banksi), Citrus red mite ( Panonychus citri), European red mite ( Panonychus ulmi), McDaniel mite ( Tetranychus mcdanieli), Pacific spider mite ( Tetranychus pacificus), Strawberry spider mite ( Tetranychus urticae), Spruce spider mite ( Oligonychus ununguis), Sugi spider mite ( Oligonychus nondonensisi), and Tetranychus evansi.

In specific embodiments, the bacterial strains provided herein are those that control one or more insect or insect pests. For example, the various bacterial strains provided herein target one or more insect pests that cause damage to plants. For example, any of the bacterial strain provided herein or active variant thereof can have insecticidal activity against one, two, three, four, five, or more insect pests described herein.

In some embodiments, the compositions and methods provided herein control nematode plant pests. Nematodes include parasitic nematodes such as root-knot, cyst, and lesion nematodes, including of the species Meloidogyne such as the Southern Root-Knot nematode (Meloidogyne incognita), Javanese Root- Knot nematode ( Meloidogyne javanica), Northern Root-Knot Nematode ( Meloidogyne hapla) and Peanut Root-Knot Nematode (Meloidogyne arenaria); nematodes of the species Ditylenchus such as Ditylenchus destructor and Ditylenchus dipsaci; nematodes of the species Pratylenchus such as the Cob Root-Lesion Nematode (Pratylenchus penetrans), Chrysanthemum Root-Lesion Nematode (Pratylenchus fallax), Pratylenchus coffeae, Pratylenchus loosi and Walnut Root-Lesion Nematode (Pratylenchus vulnus ); Nematodes of the species Globodera such as Glohodera rostochiensis and Glohodera pallida,' Nematodes of the species Heterodera such as Heterodera glycines (soybean cyst nematode); Heterodera schachtii (beet cyst nematode); Heterodera avenae (cereal cyst nematode); Nematodes of the species Aphelenchoides such as the Rice White-tip Nematode (Aphelenchoides hesseyi), Aphelenchoides ritzemabosi and Aphelenchoides fragariae,' Nematodes of the species Aphelenchus such as Aphelenchus avenae, Nematodes of the species Radopholus, such as the Burrowing-Nematode (Radopholus similis); Nematodes of the species Tylenchulus such as Tylenchulus semipenetrans; Nematodes of the species Rotylenchulus such as Rotylenchulus reniformis,' Nematodes living in trees such as Bursaphelenchus xylophilus and the Red Ring Nematode (Bursaphelenchus cocophilus) etc. and Globodera spp.; particularly members of the cyst nematodes, including, but not limited to Globodera rostochiensis and Globodera pailida (potato cyst nematodes); Spiral (Helicotylenchus spp.),' Burrowing (Radopholus similis),' Bulb and stem (Ditylenchus dipsaci),' Reniform (Rotylenchulus reniformis),' Dagger (Xiphinema spp.); Bud and leaf (Aphelenchoides spp.); and Pine Wilt Disease (Bursaphelenchus xylophilus). Lesion nematodes include Pratylenchus spp. The term "nematode" encompasses eggs, larvae, juvenile and mature forms of nematodes.

Bacterial strains or active variants thereof and/or a composition derived therefrom can be tested for pesticidal activity against a pest in any developmental stage, including early developmental stages, e.g., as larvae or other immature forms. For example, larvae of insect pests may be reared in total darkness at from about 20 °C to about 30 °C and from about 30% to about 70% relative humidity. Bioassays may be performed as described in Czapla and Lang (1990) J. Econ. Entomol. 83 (6): 2480-2485. Methods of rearing insect larvae and performing bioassays are well known to one of ordinary skill in the art.

In specific embodiments, the bacterial strains provided herein are those that control one or more nematode or nematode pests. For example, the various bacterial strains provided herein control one or more nematode pests that cause damage to plants. For example, any of the bacterial strain provided herein or active variant thereof can have nematicidal activity against one, two, three, four, five, or more nematode pests described herein.

In one non-limiting embodiment, the bacterial strain, active variant thereof, and/or a composition derived therefrom provided herein can be employed to decrease or reduce the level of a plant pest. The term “pests” includes but is not limited to, insects, fungi, fungal-like organisms, bacteria, nematodes, viruses, viroids, protozoan pathogens, and the like. By "pest resistance" is intended that the bacterial strain, active variant thereof, and/or a composition derived therefrom provided herein can inhibit (inhibit growth, feeding, fecundity, or viability), suppress (suppressing growth, feeding, fecundity, or viability), reduce (reduce the pest infestation, reduce the pest feeding activities on a particular plant) or kill (cause the morbidity, mortality, or reduced fecundity of) a pest, such as an insect pest. By “a plant susceptible to a pest” is meant that a pest is able to infect or damage the plant. For example, a plant susceptible to a pest can be susceptible to damage caused by a insect, or nematode pest as disclosed elsewhere herein.

In further embodiments, the bacterial strains or active variants thereof (i.e., AIP015329, AIP075655, or AIP059286 or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores, and/or a composition derived from any one of AIP015329, AIP075655, or AIP059286 or an active variant of any thereof) control at least one, two, three, four, five, or more of the plant pathogens and/or plant pests described herein. In specific embodiments, the bacterial strains AIP015329, AIP075655, or AIP059286 or active variants thereof, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores, and/or a composition derived from any one of AIPO 15329, AIP075655, or AIP059286 or an active variant of any thereof control at least one plant pest, plant pathogen, and/or plant disease described herein.

In further embodiments, the bacterial strains or active variants thereof (i.e., AIP015329, AIP075655, or AIP059286 or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores, and/or a composition derived from any one of AIP015329, AIP075655, or AIP059286 or an active variant of any thereof) control at least one, two, three, four, five, or more plant diseases or diseases caused by plant pathogens or plant pests such as insect pests, selected from the group consisting of Asian Soybean Rust (ASR), gray mold, leaf spot, Frogeye Leaf Spot, Early Blight, Damping off complex, Brown Patch, black scurf, root rot, belly rot, Sheath Blight, Powdery Mildew, Anthracnose, Black Sigatoka, Anthracnose leaf spot, Downy Mildew, Pythium Blight, Late Blight, Fusarium Head Blight, sudden death syndrome (SDS), Fusarium Wilt, Com Stalk Rot, Brown Rust, Black Rust, Yellow Rust,

Wheat Rust, Rust, Apple Scab, Post-bloom Fruit Drop, Gummy Stem Blight, Greasy Spot, Com Stalk Rot, Cherry Blossom Blight, Damping Off, Fire Blight, Citrus Greening Disease, Clubroot, Verticillium Wilt, and Brown Rot.

B. Methods of Treating or Preventing Plant Disease

Provided herein are methods of treating or preventing a plant disease comprising applying to a plant having a plant disease or at risk of developing a plant disease an effective amount of at least one bacterial strain provided herein or an active variant thereof, and/or a composition derived therefrom wherein the bacterial strain controls a plant pest that causes the plant disease. The plant pest may be a vims, viroid, bacteria, nematode, fungus, fungal-like organism (such as and including an Oomycete, plasmodiophorid, and a member of the Phytomyxea), insect, or protozoan pathogen. Also provided herein are methods of controlling a plant pest or preventing plant damage caused by a plant pest comprising applying to a plant an effective amount of at least one bacterial strain provided herein or an active variant thereof, and/or a composition derived therefrom wherein the bacterial strain controls a plant pest. Also provided herein are methods of reducing susceptibility to a plant pest and/or increasing resistance to a plant pest comprising applying to a plant having a plant disease or damage or at risk of developing a plant disease or damage caused by a plant pest an effective amount of at least one bacterial strain provided herein or an active variant thereof, and/or a composition derived therefrom wherein the bacterial strain controls the plant pest. In certain embodiments, the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom may comprise at least one of AIP015329, AIP075655, or AIP059286, or an active variant of any thereof; or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP015329, AIP075655, or AIP059286, or an active variant or any thereof. In some embodiments, the effective amount of the bacterial strain or active variant thereof and/or a composition derived therefrom comprises at least about 10 ⁴ to 10 ¹⁶ CFU per hectare, at least about 10 ¹² to 10 ¹⁶ CFU per hectare, or least about 10 ⁵ to 10 ¹¹ CFU per hectare. In some embodiments, the composition is derived from a bacterial strain provided herein or active variant thereof which may comprise a cell of at least one of AIP015329, AIP075655, or AIP059286 or an active variant of any thereof; or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIPO 15329, AIP075655, or AIP059286 or an active variant of any thereof.In some methods, a bacterial strain provided herein or an active variant therof, and/or a composition derived therefrom is an agent that treats or prevents one, two, three, four, five or more plant diseases, infections, or infestations by plant pests. In other methods, a bacterial strain provided herein or an active variant thereof, and/or a composition derived therefrom is an antipesticidal agent that treats or prevents one, two, three, four, five or more fungal plant diseases, diseases caused by fungal-like pathogens, diseases caused by bacterial pathogens, or infections or infestations caused by insect pests or nematode pests. A bacterial strain provided herein or an active variant therof, and/or a composition derived therefrom can be employed with any plant species susceptible to a plant disease of interest and/or susceptible to a plant pest of interest.

Examples of diseases causes by fungal, fungal-like, bacterial, or other plant pests described herein are provided in Table 1. Also provided are non-limiting exemplary crop species that are susceptible to the plant diseases caused by the pathogens. For example, Table 1 shows that Bortrytis cinerea causes gray mold on all flowering crops. Therefore, a bacterial strain provided herein or active variant therof, and/or a composition derived therefrom that controls Bortrytis cinerea can be applied to a plant having gray mold or at risk of developing gray mold in order to treat or prevent gray mold in the plant. Similarly, Table 1 shows that Rhizoctonia solani causes Damping off complex in com, Damping off complex in soybean, Brown Patch in turf, and Damping off complex in ornamentals. Therefore, a bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom that controls Rhizoctonia solani can be applied to a plant having Damping off complex and/or brown patch or at risk of developing Damping off complex and/or brown patch in order to treat or prevent Damping off complex and/or brown patch in the plant. In yet another example, Table 1 shows that Colletotrichum cereale, Apiognomonia errabunda, Apiognomonia veneta, Colletotrichum gloeosporiodes, Discula fraxinea cause Anthracnose leaf spot. Therefore, a bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom that controls one or more of Colletotrichum cereale, Apiognomonia errabunda, Apiognomonia veneta, Colletotrichum gloeosporiodes, Discula fraxinea can be applied to a plant having Anthracnose leaf spot or at risk of developing Anthracnose leaf spot in order to treat or prevent Anthracnose leaf spot in the plant.

Table 1

The term “treat” or “treating” or its derivatives includes substantially inhibiting, slowing, or reversing the progression of a condition, substantially ameliorating symptoms of a condition or substantially preventing the appearance of symptoms or conditions brought about by the pathogen or pest that causes the plant disease.

The terms “controlling” and “protecting a plant from a pathogen” refers to one or more of inhibiting or reducing the growth, germination, reproduction, and/or proliferation of a pathogen of interest; and/or killing, removing, destroying, or otherwise diminishing the occurrence, and/or activity of a pathogen of interest. As such, a plant or plant part treated with a bacterial strain provided herein may show a reduced disease severity or reduced disease development in the presence of plant pathogens by a statistically significant amount. The bacterial strains, or combinations thereof, provided herein can reduce the growth, germination, reproduction, and/or proliferation of a pathogen of interest on a plant or plant part in a field or area of cultivation or following removal of the plant or plant part from a field or area of cultivation.

The term “prevent” and is variations means the countering in advance of bacterial, fungal, viral, insect or other pest growth, proliferation, infestation, spore germination, and hyphae growth. In this instance, the composition is applied before exposure to the pathogens or plant pests.

The term “ameliorate” and “amelioration” relate to the improvement in the treated plant condition brought about by the compositions and methods provided herein. The improvement can be manifested in the forms of a decrease in pathogen or pest growth and/or an improvement in the diseased plant height, weight, number of leaves, root system, or yield. In general, the term refers to the improvement in a diseased plant physiological state.

The term "inhibit" and all variations of this term is intended to encompass the restriction or prohibition of bacterial, fungal, viral, nematode, insect, or any other pest growth, as well as spore germination. The term "eliminate" relates to the substantial eradication or removal of bacteria, fungi, viruses, nematodes, insects, or any other pests by contacting them with the composition of the invention, optionally, according to the methods of the invention described below.

The terms "delay", "retard" and all variations thereof are intended to encompass the slowing of the progress of bacterial, fungal, viral, nematode, insect, or any other pest growth, and spore germination. The expression "delaying the onset" is interpreted as preventing or slowing the progression of bacterial, fungal, viral, nematodes, insect, or any other pest growth, infestation, infection, spore germination and hyphae growth for a period of time, such that said bacterial, fungal, viral, nematode, insect, or any other pest growth, infestation, infection, spore germination and hyphae growth do not progress as far along in development, or appear later than in the absence of the treatment according to the invention.

A plant, plant part, or area of cultivation treated with a bacterial strain provided herein or an active variant thereof, and/or a composition derived therefrom may show a reduced disease severity or reduced disease development in the presence of plant pathogens or plant pest by a statistically significant amount. A reduced disease severity or reduced disease development can be a reduction of about 10% to about 20%, about 20% to about 30%, about 30% to about 40%, about 40% to about 50%, about 50% to about 60%, about 60% to about 70%, about 70% to about 80%, about 80% to about 90%, or about 90% to about 100% when compared to non-treated control plants. In other instances, the plant treated with a bacterial strain provided herein or an active variant thereof, and/or a composition derived therefrom may show a reduced disease severity or reduced disease development in the presence of plant pathogen of at least about 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%,

29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%,

47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%,

65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%,

83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or about

100% greater when compared to non-treated control plants. Methods for assessing plant disease severity are known, and include, measuring percentage of diseased leaf area (Godoy etal. (2006) Fitopatol. Bras. 31(1) 63-68 or by measuring growth of the pathogen, for example uredinia counts for Phakops ora pachyrhiz (see Example 5).

A plant, plant part, or area of cultivation treated with a bacterial strain provided herein or an active variant thereof, and/or a composition derived therefrom may show a reduction of plant pathogens, including fungal and fungal -like pathogens. A reduction of plant pathogens can be a reduction of about 10% to about 20%, about 20% to about 30%, about 30% to about 40%, about 40% to about 50%, about 50% to about 60%, about 60% to about 70%, about 70% to about 80%, about 80% to about 90%, or about 90% to about 100% when compared to non-treated control plants or plant parts. In other instances, the plant or plant part treated with a bacterial strain provided herein or an active variant thereof, and/or a composition derived therefrom may show a may show a reduction of plant pathogens of at least about 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%,

52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%,

70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%,

88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or about 100% greater when compared to non-treated control plants. Methods for measuring the number of plant pathogens are known, and include contacting plants with one or more pests and determining the plant's ability to survive and/or cause the death of the pests.

By "antipathogenic compositions" or “antipathogenic” is intended that the compositions are capable of suppressing, controlling, preventing and/or killing the invading pathogenic organism. In specific embodiments, an antipathogenic composition reduces the disease symptoms resulting from pathogen challenge by a statistically significant amount, including for example, at least about 10% to at least about 20%, at least about 20% to about 50%, at least about 10% to about 60%, at least about 30% to about 70%, at least about 40% to about 80%, or at least about 50% to about 90% or greater. Hence, the methods of the invention can be utilized to protect plants from disease, particularly those diseases that are caused by plant pathogens.

Assays that measure antipathogenic activity are commonly known in the art, as are methods to quantitate disease resistance in plants following pathogen infection. See, for example, U.S. Patent No. 5,614,395, herein incorporated by reference. Such techniques include, measuring overtime, the average lesion diameter, the pathogen biomass, and the overall percentage of decayed plant tissues. For example, a plant either expressing an antipathogenic polypeptide or having an antipathogenic composition applied to its surface shows a decrease in tissue necrosis (i.e., lesion diameter) or a decrease in plant death following pathogen challenge when compared to a control plant that was not exposed to the antipathogenic composition. Alternatively, antipathogenic activity can be measured by a decrease in pathogen biomass.

For example, a plant expressing an antipathogenic polypeptide or exposed to an antipathogenic composition is challenged with a pathogen of interest. Over time, tissue samples from the pathogen-inoculated tissues are obtained and RNA is extracted. The percent of a specific pathogen RNA transcript relative to the level of a plant specific transcript allows the level of pathogen biomass to be determined. See, for example, Thomma el al. (1998) Plant Biology 95: 15107-15111, herein incorporated by reference.

Furthermore, in vitro antipathogenic assays include, for example, the addition of varying concentrations of the antipathogenic composition to paper disks and placing the disks on agar containing a suspension of the pathogen of interest. Following incubation, clear inhibition zones develop around the discs that contain an effective concentration of the antipathogenic polypeptide (Liu el al. (1994) Plant Biology 91: 1888-1892, herein incorporated by reference). Additionally, microspectrophotometrical analysis can be used to measure the in vitro antipathogenic properties of a composition (Hu el al. (1997) Plant Mol. Biol. 34:949-959 and Cammue et al. (1992) J. Biol. Chem. 267: 2228-2233, both of which are herein incorporated by reference). C. Methods of Inducing Disease and/or Pest Resistance in Plants and/or for Improving Plant

Health and/or Improving an Agonomic Trait of Interest

Compositions and methods for inducing disease resistance in a plant to plant pathogens are also provided. Accordingly, the compositions and methods are also useful in protecting plants against fungal pathogens, viruses, nematodes, and insects. Provided herein are methods of inducing disease resistance against a plant pathogen comprising applying to a plant that is susceptible to a plant disease caused by the plant pathogen an effective amount of at least one bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom. In certain embodiments, a bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom may comprise at least one of AIP015329, AIP075655, or AIP059286, or an active variant of any thereof; or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP015329, AIP075655, or AIP059286, or an active variant of any thereof. In certain embodiments, the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom promotes a defensive response to the pathogen that causes the plant disease. In some embodiments, the effective amount of a bacterial strain provided herein or active variant thereof comprises at least about 10 ⁴ to 10 ¹⁶ CFU per hectare. In some embodiments, the effective amount of a bacterial strain provided herein or active variant thereof comprises at least about 10 ⁵ to 10 ¹² CFU per hectare. In some embodiments, the effective amount of a bacterial strain provided herein or active variant thereof comprises at least about 10 ¹² to 10 ¹⁶ CFU per hectare. In some embodiments, the effective amount of the bacterial strain or active variant thereof comprises at least about 10 ⁶ to 10 ¹⁸ cells per hectare. In some embodiments, the effective amount of the bacterial strain or active variant thereof comprises at least about 10 ¹⁴ to 10 ¹⁸ cells per hectare. In some embodiments, the effective amount of the bacterial strain or active variant thereof comprises at least about 10 ⁹ to 10 ¹³ cells per hectare. In some embodiments, the effective amount of the bacterial strain or active variant thereof comprises at least about 10 ⁷ to 10 ¹⁴ cells per hectare.

A defensive response in the plant can be triggered after applying a bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom to the plant, but prior to pathogen challenge and/or after pathogen challenge of the plant treated with a bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom.

In some methods, a bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom induces resistance to one, two, three, four, five or more plant pathogens described herein. In other methods, a bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom induces resistance to one, two, three, four, five or more fungal plant pathogens described herein.

By "disease resistance" is intended that the plants avoid the disease symptoms that result from plant- pathogen interactions. That is, pathogens are prevented from causing plant diseases and the associated disease symptoms, or alternatively, the disease symptoms caused by the pathogen are minimized or lessened as compared to a control. By "pest resistance" is intended that the plants avoid the symptoms that result from infection of a plant by a pest. That is, pests are prevented from causing plant diseases and the associated disease symptoms, or alternatively, the disease symptoms caused by the pest are minimized or lessened as compared to a control. Further provided are methods of improving plant health and/or improving an agronomic trait of interest comprising applying to a plant an effective amount of at least one bacterial strain provided herein or an active variant thereof, and/or a composition derived therefrom or an active variant thereof. In certain embodiments, a bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom may comprise at least one of AIP015329, AIP075655, or AIP059286, or an active variant of any thereof; or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP015329, AIP075655, or AIP059286, or an active variant of any thereof. In some embodiments, the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 ⁴ to 10 ¹⁶ CFU per hectare. In some embodiments, the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 ⁵ to 10 ¹² CFU per hectare. In some embodiments, the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 ¹² to 10 ¹⁶ CFU per hectare. In some embodiments, the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 ⁶ to 10 ¹⁸ cells per hectare. In some embodiments, the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 ¹⁴ to 10 ¹⁸ cells per hectare. In some embodiments, the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 ⁹ to 10 ¹³ cells per hectare. In some embodiments, the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 ⁷ to 10 ¹⁴ cells per hectare. In some embodiments, the composition is derived from a bacteria strain provided herein or active variant thereof which may comprise a cell of at least one of AIP015329, AIP075655, or AIP059286 or an active variant of any thereof; or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP015329, AIP075655, or AIP059286 or an active variant of any thereof.

In particular embodiments, the agronomic trait of interest that is improved by the bacterial strains or active variants thereof described herein is improved plant health. By “improved plant health” is meant increased growth and/or yield of a plant, increased stress tolerance and/or decreased herbicide resistance, to name a few. Increased stress tolerance refers to an increase in the ability of a plant to decrease or prevent symptoms associated with one or more stresses. The stress can be a biotic stress that occurs as a result of damage done to plants by other living organisms such as a pathogen (for example, bacteria, viruses, fungi, parasites), insects, nematodes, weeds, cultivated or native plants. The stress can also be an abiotic stress such as extreme temperatures (high or low), high winds, drought, salinity, chemical toxicity, oxidative stress, flood, tornadoes, wildfires, radiation and exposure to heavy metals. Non-limiting examples of improved agronomic traits are disclosed elsewhere herein. In specific embodiments, an effective amount of the bacterial strain or active variant thereof, and/or a composition derived therefrom .improves plant health or improves an agronomic trait of interest by a statistically significant amount, including for example, at least about 10% to at least about 20%, at least about 20% to about 50%, at least about 10% to about 60%, at least about 30% to about 70%, at least about 40% to about 80%, or at least about 50% to about 90% or greater. D. Methods of Application to a Plant or Plant Part

A bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom is applied in an effective amount. An effective amount of a bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom is an amount sufficient to control, treat, prevent, inhibit the pathogen or pest that causes a plant disease, and/or reduce plant disease severity or reduce plant disease development. In other embodiments, the effective amount of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom is an amount sufficient to improve an agronomic trait of interest and/or to promote or increase plant health, growth or yield of a plant susceptible to a disease and/or infection by a plant pest. The rate of application of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom may vary according to the pathogen or pest being targeted, the crop to be protected, the efficacy of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom, the severity of the disease, the climate conditions, the agronomic trait of interest to improve, and the like.

Generally, the rate of bacterial strain provided herein or active variant thereof is 10 ⁴ to 10 ¹⁶ colony forming units (CFU) per hectare. In other embodiments, for a field inoculation, the rate of bacterial strain provided herein or active variant thereof application is 3 x 10 ⁷ to 1 x 10 ¹¹ colony forming units (CFU) per hectare. (This corresponds to about 1 kg to 10 kg of formulated material per hectare). In other embodiments, for a field inoculation, the rate of bacterial strain provided herein or active variant thereof application is 3 x 10 ⁷ to 1 x 10 ¹⁶ colony forming units (CFU) per hectare; about 1x10 ¹² to about 1x10 ¹³ colony forming units (CFU) per hectare, about 1x10 ¹³ to about 1x10 ¹⁴ colony forming units (CFU) per hectare, about 1x10 ¹⁴ to about 1x10 ¹⁵ colony forming units (CFU) per hectare, about 1x10 ¹⁵ to about 1x10 ¹⁶ colony forming units (CFU) per hectare, about 1x10 ¹⁶ to about 1x10 ¹⁷ colony forming units (CFU) per hectare; about 1x10 ⁴ to about 1x10 ¹⁶ colony forming units (CFU) per hectare; about 1x10 ⁵ to about 1x10 ¹³ colony forming units (CFU) per hectare; about 1x10 ⁶ to about 1x10 ¹² colony forming units (CFU) per hectare; about 1x10 ⁹ to about 1x10 ¹¹ colony forming units (CFU) per hectare; about 1x10 ⁹ to about 1x10 ¹¹ colony forming units (CFU) per hectare. In other embodiments, for a field inoculation, the rate of bacterial strain provided herein or active variant thereof application is at least about 1x10 ⁴ , about 1x10 ⁵ , about 1x10 ⁶ , about 1x10 ⁷ , about 1x10 ⁸ , about 1x10 ⁹ , about 1x10 ¹⁰ , about 1x10 ¹¹ , about 1x10 ¹² 1x10 ¹³ , about 1x10 ¹⁴ , 1x10 ¹⁵ , about 1x10 ¹⁶ , or about 1x10 ¹⁷ colony forming units (CFU) per hectare. In some embodiments, for a field inoculation, the rate of bacterial strain provided herein or active variant thereof application is at least 1x10 ⁴ to at least about 1x10 ¹⁶ CFU/hectare. In other embodiments, for a field inoculation, the rate of bacterial strain provided herein or active variant thereof application is at least 1x10 ⁷ to at least about 1x10 ¹⁴ CFU/hectare.

In some embodiments, the rate of bacterial strain provided herein or active variant thereof is 10 ⁶ to 10 ¹⁸ cells per hectare. In other embodiments, for a field inoculation, the rate of bacterial strain provided herein or active variant thereof application is 3 x 10 ⁹ to 1 x 10 ¹⁸ cells per hectare; about 1x10 ¹⁴ to about 1x10 ¹⁵ cells per hectare, about 1x10 ¹⁵ to about 1x10 ¹⁶ cells per hectare, about 1x10 ¹⁶ to about 1x10 ¹⁷ cells per hectare, about 1x10 ¹⁷ to about 1x10 ¹⁸ cells per hectare, about 1x10 ¹⁸ to about 1x10 ¹⁹ cells per hectare; about 1x10 ⁶ to about 1x10 ¹⁸ cells per hectare; about 1x10 ⁷ to about 1x10 ¹⁵ cells per hectare; about 1x10 ⁸ to about 1x10 ¹⁴ cells per hectare; about 1x10 ¹¹ to about 1x10 ¹³ cells per hectare; about 1x10 ¹¹ to about 1x10 ¹³ cells per hectare; about 1x10 ⁷ to about 1x10 ¹⁴ cells per hectare. In other embodiments, for a field inoculation, the rate of bacterial strain provided herein or active variant thereof application is at least about 1x10 ⁴ , about 1x10 ⁵ , about 1x10 ⁶ , about 1x10 ⁷ , about 1x10 ⁸ , about 1x10 ⁹ , about 1x10 ¹⁰ , about 1x10 ¹¹ , about 1x10 ¹² lxl0 ¹³ , about 1x10 ¹⁴ , 1x10 ¹⁵ , about 1x10 ¹⁶ , about 1x10 ¹⁷ , about 1x10 ¹⁸ , or about 1x10 ¹⁹ cells per hectare.

In specific embodiments, the bacterial strain provided herein or active variant thereof applied comprises the strain deposited as AIP015329, AIP075655, or AIP059286, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP015329, AIP075655, or AIP059286, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIPO 15329, AIP075655, or AIP059286 or an active variant of any thereof.

In some embodiments, the applied composition is derived from a bacterial strain or active variant thereof comprising a strain deposited as AIP015329, AIP075655, or AIP059286 or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP015329, AIP075655, or AIP059286 or an active variant of any thereof. In some embodiments, the applied composition may be a substantially pure culture, whole cell broth, supernatant, filtrate, extract, or compound derived from a bacterial strain of the invention or an active variant thereof. The applied composition may be applied alone or in combination with another substance, in an effective amount for controlling a plant pathogen or for improving an agronomic trait of interest in a plant or plant part.

An effective amount of the applied composition is the quantity of microorganism cells, supernatant, whole cell broth, filtrate, cell fraction or extract, metabolite, and/or compound alone or in combination with another pesticidal substance that is sufficient to modulate plant pest infestation or the performance of an agronomic trait of interest in the plant. The amount that will be within an effective range can be determined by laboratory or field tests by one skilled in the art.

In some embodiments, when the composition is applied directly to the seed, the effective amount is a concentration of about 0.05-25%, or about 0.1-20%, or about 0.5-15%, or about 1-10%, or about 2-5% of the active ingredient per 100 g of seed. In some embodiments, the effective amount is about 0.5-1% of the active ingredient per 100 g of seed.

In some embodiments, when the composition is applied to the soil by, for example, in furrow, the effective amount is about 0.1-50 oz. of the active ingredient per 1000 ft row. In another embodiment, the effective amount for soil application is about 1-25 oz. of the active ingredient per 1000 ft row. In another embodiment, the effective amont is about 2-20 oz, or about 3-15 oz, or about 4-10 oz, or about 5-8 oz, of the active ingredient per 1000 ft row. In yet another embodiment, the effective amount is about 14 or 28 oz of the active ingredient per 1000 ft row. Any appropriate agricultural application rate for a biocide can be applied in combination with the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom disclosed herein. Methods to assay for the effective amount of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom include, for example, any statistically significant increase in the control of the pathogen or pest targeted by the biocide. Methods to assay for such control are known. Moreover, a statistically significant increase in the control of plant health, yield and/or growth that occurs upon application of an effective amount of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom when compared to the plant health, yield and/or growth that occurs when no bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom is applied.

Further provided is a method for controlling or inhibiting the growth of a plant pest or a plant pathogen that causes plant disease by applying a composition comprising at least one bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom provided herein (i.e., AIP015329, AIP075655, or AIP059286, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores from AIP015329, AIP075655, or AIP059286, or an active variant any of thereof). By “applying” is intended contacting an effective amount of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom to a plant, area of cultivation, seed and/or weed with one or more of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom so that a desired effect is achieved. Furthermore, the application of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom can occur prior to the planting of the crop (for example, to the soil, the seed, or the plant). In a specific embodiment, the application of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom is a foliar application. Therefore, a further embodiment of the invention provides a method for controlling or inhibiting the growth of a plant pest by applying the population of bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom to an environment in which the plant pest may grow. The application may be to the plant, to parts of the plant, to the seeds of the plants to be protected, or to the soil in which the plant to be protected are growing or will grow. Application to the plant or plant parts may be before or after harvest. Application to the seeds will be prior to planting of the seeds.

In some embodiments, an effective amount of at least one bacterial strain provided herein or active variant thereof provided herein is used as a foliar application to control or inhibit growth of one or more pathogens selected from the group consisting of Alternaria spp., Alternaria solani, Colletotrichum spp., Erysiphe spp., Mycosphaerella spp., Phomopsis spp., Podosphaera spp., Cercospora spp., Botrytis spp., Uncinula spp., Erwinia spp., Pseudomonas spp., and Xanthomonas spp.

In other embodiments, an effective amount of at least one bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom provided herein is applied to the soil in which the plant to be protected are growing or will grow to control or inhibit growth of one or more pathogens selected from the group consisting of Rhizoctonia spp., Rhizoctonia solani, Fusarium spp., Sclerotium spp., Sclerotinia spp., Sclerotinia sclerotiorum, Phytopthora spp., and Pythium spp.

In some embodiments, an effective amount of at least one bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom provided herein is applied to the plant after harvest to control or inhibit growth of one or more pathogens.

In some embodiments, the plant or plant part has been harvested or otherwise removed from the field or area of cultivation. Methods are provided for controlling a plant pest after harvest, also referred to as post-harvest. In some embodiments, the plant pest is a plant pathogen. In further embodiments, the plant pathogen is a my cotoxin-producing fungus. My cotoxins are known to be produced by Aspergillus spp., Penicillium spp., Fusarium spp., and Claviceps spp. (Liu etal. (2020) Comprehensive Reviews in Food Science and Food Safety 19: 1521-1560, incorporated by reference herein). Post-harvest plant pathogens include mycotoxin-producing fungi and rotting fungal and bacterial pathogens, including Aspergillus spp., Botrytis spp., Fusarium spp., Phacidiopynis spp., Sphaeropsis spp., Botryosphaeria spp., Rhizopus spp., Claviceps spp., Colletotrichum spp., Geotricum spp., Diaporthe spp.. Mucor spp., Lasiodiplodis spp., Neofahrea spp.. Monilinia spp., Gilhertella spp., Penicillium spp., Erwinia spp., Pectohacterium spp., and Brenneria spp.

An effective amount of a composition comprising at least one bacterial strain provided herein or active variant thereof can be applied to a plant or plant part prior to harvesting or after the plant or plant part has been harvested. As used herein, the term harvesting refers to the removal of a plant or plant part from the ground or other area of cultivation and can also refer to removal of a plant part from a plant that remains in the ground or other area of cultivation. The plant part may be a fruit, which may be harvested from a tree, bush, or vine, or it may be the edible portion of a vegetable crop such as a leaf, tuber, or root. Examples of plant parts include pome fruit, citrus, stone fruit, berries, tomatoes, peppers, melons, and tropical fruit. In some embodiments, the plant part is the grain or seed of the plant. Methods of post-harvest application include coating, spraying (high volume or low volume), fogging, thermofogging, drenching, dipping, flooding, foaming, brushing, or dusting the harvested plant or plant part with a composition comprising a bacterial strain described herein or a variant thereof. Other methods of post-harvest application include using paper wraps or box liners that have been treated with a composition comprising or derived from a bacterial strain described herein or a variant thereof. The composition comprising or derived from a bacterial strain described herein or a variant thereof may be aqueous or a wax-oil emulsion. In some embodiments, the composition is a fruit coating further comprising non-emulsified mineral oil, emulsified mineral oil, polyethylene, vegetable oil, camauba, shellac, a wood rosin blend, or a combination of any these.

In certain embodiments, the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom may comprise at least one of AIP015329, AIP075655, AIP059286, or an active variant of any thereof; or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP015329, AIP075655, AIP059286, or an active variant of any thereof. Various effective amounts of bacterial strain provided herein or active variant thereof are disclosed elsewhere herein and in one, non-limiting example, the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 ⁴ to 10 ¹² CFU/g, about 10 ¹² to 10 ¹⁶ CFU/g, about 10 ⁴ to 10 ¹² CFU/mL, about 10 ¹² to 10 ¹⁶ CFU/mL, or equivalent measure of bacterial concentration. In some embodiments, the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 ⁴ to 10 ¹² cells/100 gallons of water, about 10 ⁸ to 10 ¹⁴ cells/100 gallons of water, about 10 ⁷ to 10 ¹⁰ cells/100 gallons of water, or about 10 ⁹ to 10 ¹⁴ cells/100 gallons of water. In some embodiments, the composition further comprises at least one synthetic pesticide, such as for example imazalil, pyrimethanil, fludioxonil, azoxystrobin, propiconazole, tebucanozole, difenoconazole, or any synthetic pesticide recited elsewhere herein. In high volume applications, for example by a T-jet, a bacterial strain of the invention or a variant thereof may be supplied at about 10 ⁴ to 10 ¹² CFU/mL or 10 ⁶ to 10 ¹⁴ cells/mL at 100-200 gallons/ton of fruit. In low volume applications, for example by controlled droplet application, a bacterial strain of the invention or a variant thereof may be supplied at about 10 ⁴ to 10 ¹² CFU/mL or 10 ⁶ to 10 ¹⁴ cells/mL at 8-30 gallons/ton of fruit.

In specific embodiments, the application of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom (i.e., AIP015329, AIP075655, AIP059286, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP015329, AIP075655, AIP059286, or an active variant of any thereof, and/or a composition derived therefrom) is applied to the leaves of a soybean plant. The timing of application can vary depending on the conditions and geographical location. In specific embodiments, the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom is applied at the R1 (beginning flowering stage) of soybean development or may be applied earlier depending on disease onset and the disease severity.

In other embodiments, the biocide to a crop, area of cultivation, or field it is intended that one or more of a particular field, plant crop, seed and/or weed is treated with one or more of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom and one or more biocide so that a desired effect is achieved.

Various methods are provided for controlling a plant pest that causes a plant disease in an area of cultivation containing a plant susceptible to the plant disease. The method comprises planting the area of cultivation with seeds or plants susceptible to the plant disease or pest; and applying to the plant susceptible to the disease or pest, the seed or the area of cultivation of the plant susceptible to the plant disease or pest an effective amount of at least one bacterial strain provided herein or active variant thereof, and/or a composition derived thcrcfrom (i.c.. AIP015329, AIP075655, AIP059286, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores from AIP015329, AIP075655, AIP059286, or an active variant thereof), wherein the effective amount of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom controls the plant disease without significantly affecting the crop. In some embodiments, the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 ⁴ to 10 ¹⁶ CFU per hectare. In some embodiments, the effective amount comprises at least about 10 ⁵ to 10 ¹² colony forming units (CFU) per hectare. In other embodiments, the effective amount comprises at least about 10 ¹² to 10 ¹⁶ colony forming units (CFU) per hectare. In some embodiments, the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 ⁶ to 10 ¹⁸ cells per hectare. In some embodiments, the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 ¹⁴ to 10 ¹⁸ cells per hectare. In some embodiments, the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 ⁹ to 10 ¹³ cells per hectare. In some embodiments, the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 ⁷ to 10 ¹⁴ cells per hectare. In some embodiments, the composition is derived from a bacterial strain provided herein or active variant thereof and may comprise a cell of at least one of AIP015329, AIP075655, or AIP059286 or an active variant of any thereof; or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP015329, AIP075655, or AIP059286 or an active variant of any thereof.

Further provided is a method for growing a plant susceptible to a plant disease or plant pest. The method comprises applying to a plant susceptible to the disease or pest, a seed, or an area of cultivation of the plant susceptible to the disease or pest an effective amount of a composition comprising at least one bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom. In certain embodiments, the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom may comprise AIP015329, AIP075655, or AIP059286, or an active variant of any thereof; or a spore, or a forespore or a combination of cells, forespores and/or spores of AIP015329, AIP075655, or AIP059286, or an active variant of any thereof. Various effective amounts of bacterial strain provided herein or active variant thereof are disclosed elsewhere herein and in one, non-limiting example, the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 ⁴ to 10 ¹⁶ colony forming units (CFU) per hectare, at least about 10 ⁵ to 10 ¹² colony forming units (CFU) per hectare, or at least about 10 ¹² to 10 ¹⁶ colony forming units (CFU) per hectare. In some embodiments, the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 ⁶ to 10 ¹⁸ cells per hectare, at least about 10 ¹⁴ to 10 ¹⁸ cells per hectare, at least about 10 ⁹ to 10 ¹³ cells per hectare, or at least about 10 ⁷ to 10 ¹⁴ cells per hectare.

Methods are provided for controlling a plant pest on a plant or plant part by applying to the plant or plant part an effective amount of a composition comprising at least one bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom. Methods are also provided for controlling a plant pest by contacting said pest with an effective amount of a composition comprising at least one bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom. Various effective amounts of bacterial strain provided herein or an active variant thereof are disclosed elsewhere herein. In one, non-limiting example, the effective amount of a bacterial strain provided herein or active variant thereof comprises at least about 10 ⁴ to 10 ¹⁶ colony forming units (CFU) per hectare, at least about 10 ⁴ to 10 ¹² colony forming units (CFU) per hectare, or at least about 10 ¹² to 10 ¹⁶ colony forming units (CFU) per hectare. The composition comprising the bacterial strain or active variant thereof can be a solid or liquid composition or formulation. The plant or plant part need not be actively growing in order for the bacterial strain to effectively control the plant pest.

Methods for increasing plant yield are provided. The "yield" of the plant refers to the quality and/or quantity of biomass produced by the plant. By "biomass" is intended any measured plant product. An increase in biomass production is any improvement in the yield of the measured plant product. An increase in yield can comprise any statistically significant increase including, but not limited to, at least a 1% increase, at least a 3% increase, at least a 5% increase, at least a 10% increase, at least a 20% increase, at least a 30%, at least a 50%, at least a 70%, at least a 100% or a greater increase in yield compared to a plant not exposed to the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom. A method for increasing yield in a plant is also provided and comprises applying to a crop or an area of cultivation an effective amount of a composition comprising at least one bacterial strain comprising AIP015329, AIP075655, AIP059286, or an active variant of any thereof, a spore or a forespore or a combination of cells, forespores and/or spores from any one of AIP015329, AIP075655, AIP059286, or an active variant of any thereof, wherein said effective amount comprises at least about 10 ⁴ to 10 ¹⁶ colony forming units (CFU) per hectare, at least about 10 ⁵ to 10 ¹² colony forming units (CFU) per hectare, or at least about 10 ¹² to 10 ¹⁶ colony forming units (CFU) per hectare, and wherein said composition controls a plant pathogen or other plant pest, thereby increasing yield. In some embodiments, said effective amount comprises at least about 10 ⁶ to 10 ¹⁸ cells per hectare, at least about 10 ¹⁴ to 10 ¹⁸ cells per hectare, at least about 10 ⁹ to 10 ¹³ cells per hectare, or at least about 10 ⁷ to 10 ¹⁴ cells per hectare. A method for increasing yield in a plant is also provided which comprises applying to a crop or an area of cultivation an effective amount of a composition derived from at least one bacterial strain comprising AIP015329, AIP075655, AIP059286, or an active variant of any thereof, a spore or a forespore or a combination of cells, forespores and/or spores from any one of AIP015329, AIP075655, AIP059286, or an active variant of any thereof, wherein said composition controls a plant pest, thereby increasing yield.

As used herein, an “area of cultivation” comprises any region in which one desires to grow a plant. Such areas of cultivations include, but are not limited to, a field in which a plant is cultivated (such as a crop field, a sod field, a tree field, a managed forest, a field for culturing fruits and vegetables, etc.), a greenhouse, a growth chamber, etc.

Further provided is a coated seed which comprises a seed and a coating on the seed, wherein the coating comprises at least one bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom. Such coating of the seed may also be referred to as a seed treatment. In certain embodiments, the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom may comprise at least one of AIP015329, AIP075655, AIP059286, or an active variant of any thereof; or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP015329, AIP075655, AIP059286, or an active variant of any thereof. In certain embodiments, said bacterial strain provided herein or active variant thereof is present on the seed at about 10 ⁵ CFU/100 lbs of seed to about 10 ⁷ CFU/100 lbs of seed, at about 10 ⁴ CFU/100 lbs of seed to about 10 ⁸ CFU/100 lbs of seed, at about 10 ⁴ CFU/100 lbs of seed to about 10 ⁵ CFU/100 lbs of seed, at about 10 ⁵ CFU/100 lbs of seed to about 10 ⁶ CFU/100 lbs of seed, at about 10 ⁶ CFU/100 lbs of seed to about 10 ⁷ CFU/100 lbs of seed, or at about 10 ⁷ CFU/100 lbs of seed to about 10 ⁸ CFU/100 lbs of seed. In some embodiments, said bacterial strain provided herein or active variant thereof is present on the seed at about 10 ⁷ cells/100 lbs of seed to about 10 ¹² cells/ 100 lbs of seed, at about 10 ⁸ cells/ 100 lbs of seed to about 10 ¹³ cells/ 100 lbs of seed, at about 10 ¹⁰ cells/100 lbs of seed to about 10 ¹¹ cells/100 lbs of seed, at about 10 ⁷ cells/100 lbs of seed to about 10 ¹⁴ cells/100 lbs of seed, at about 10 ⁸ cells/100 lbs of seed to about 10 ¹⁰ cells/100 lbs of seed, at about 10 ⁹ cells/100 lbs of seed to about 10 ¹³ cells/100 lbs of seed, at about 10 ⁸ cells/100 lbs of seed to about 10 ¹³ cells/100 lbs of seed, at about 10 ⁸ cells/100 lbs of seed to about 10 ¹⁴ cells/100 lbs of seed, or at about 10 ⁷ cells/100 lbs of seed to about 10 ¹⁴ cells/100 lbs of seed. The seed coating can be applied to any seed of interest (i.e., for a monocotyledonous plant or a dicotyledonous plant). The term “seed” may refer to a seed or a propagule which is capable of producing a plant. Various plants of interest are disclosed elsewhere herein.

The seed coating may improve the health of the seed prior to being placed into a germination media, for example soil. In some embodiments, the seed coating may improve the health of the germinating seedling compared to a germinating seedling from a seed that does not have a seed coating. The seed coating may control plant pests, such as nematodes (for example Meloidogyne spp., Globodera spp., and Heterodera spp.), fungal, fungal-like, or bacterial pathogens, that infect seed or germinating seedlings. The seed coating may control pathogens that cause diseases such as damping off, vascular wilts, or rot. Such pathogens include Fusarium spp., Pythium spp., Rhizoctonia spp., Phytophthora spp., and Verticillium spp.

A seed coating can further comprise at least at least one nutrient, at least one herbicide or at least one pesticide, or at least one biocide. See, for example, US App Pub. 20040336049, 20140173979, and 20150033811. In some embodiments, the seed coating further comprises a pesticide, fungicide, nematicide, bactericide, insecticide, or an herbicide, such as those recited elsewhere herein.

A plant or plant part is provided having at least one bacterial strain disclosed herein or active variant thereof, and/or a composition derived therefrom applied to the surface of the plant or plant part. The bacterial strain, or active variant thereof applied to the surface of the plant or plant part can be in the form a composition or formulation as disclosed elsewhere herein. In specific embodiments the bacterial strain provided herein or active variant thereof is applied to a plant that has been removed from the field or area of cultivation or applied to a plant part that has been removed from the plant. In certain embodiments, the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom may comprise at least one of AIP015329, AIP075655, AIP059286, or an active variant of any thereof; or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP015329, AIP075655, AIP059286, or an active variant of any thereof. In certain embodiments, said bacterial strain provided herein or active variant thereof is applied to the plant or plant part at a concentration of about 10 ⁴ to 10 ¹² CFU/g, about 10 ¹² to 10 ¹⁶ CFU/g, about 10 ⁶ to 10 ¹⁴ cells/g, about 10 ¹⁰ to 10 ¹⁸ cells/g, about 10 ⁴ to 10 ¹² CFU/mL, about 10 ¹² to 10 ¹⁶ CFU/mL, about 10 ⁷ to 10 ¹⁴ cells/mL, about 10 ¹⁰ to 10 ¹⁸ cells/mL, or equivalent measure of bacterial concentration.

In other embodiments, a plant of interest (i.e., plant susceptible to the plant disease), a plant part of interest, and/or the area of cultivation comprising the plant, can be treated with a combination of an effective amount of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom and an effective amount of a biocide. By “treated with a combination of’ or “applying a combination of’ a bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom and a biocide to a plant, plant part, area of cultivation or field it is intended that one or more of a particular field, plant, plant part, and/or weed is treated with an effective amount of one or more of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom and one or more biocide so that a desired effect is achieved. Furthermore, the application of one or both of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom and the biocide can occur prior to the planting of the crop (for example, to the soil, or the plant) and/or after harvesting the crop. Moreover, the application of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom and the biocide may be simultaneous or the applications may be at different times (sequential), so long as the desired effect is achieved.

In one non-limiting embodiment, the active variant comprises a bacterial strain provided herein that is resistance to one or more biocide. In specific embodiments, the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom (i.e., AIP015329, AIP075655, AIP059286, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores from AIP015329, AIP075655, AIP059286, or an active variant of any thereof) is resistant to glyphosate. In such methods, a plant, crop, or area of cultivation is treated with a combination of an effective amount of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom that is resistant to glyphosate and an effective amount of glyphosate, wherein the effective amount of glyphosate is such as to selectively control weeds while the crop is not significantly damaged.

In another non-limiting embodiment, the active variant comprises a bacterial strain provided herein that is resistant to glufosinate. In such methods, a plant, crop, or area of cultivation is treated with a combination of an effective amount of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom that is resistant to glufosinate and an effective amount of glufosinate, wherein the effective amount of glufosinate is such as to selectively control weeds while the crop is not significantly damaged. In such embodiments, the effective amount of the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom js sufficient to result in a statistically significant increase in plant health, yield, and/or growth when compared to the plant health, yield, and/or growth that occurs when the same concentration of a bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom that was not modified to be resistant to glufosinate is applied in combination with the effective amount of the glufosinate or active derivative thereof. In a further embodiment, a bacterial strain provided herein or active variant therof, and/or a composition derived therefrom comprises an effective amount of AIP015329, AIP075655, AIP059286, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP015329, AIP075655, AIP059286, or an active variant of any thereof.

V. Biocides for Use in Combination with the Bacterial Strains provided herein or active variant thereof, and/or a composition derived therefrom

As discussed elsewhere herein, the bacterial strain provided herein or active variant therof, and/or a composition derived therefrom can be used in combination with a biocide (i.e., an herbicide, fungicide, pesticide, or other crop protection chemical). In such instances, the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom is compatible with the biocide of interest. In some embodiments, the bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom is provided as a stable formulation which further comprises a herbicide, fungicide, bactericide, nematicide, pesticide, insecticide or other crop protection chemical.

Herbicides that can be used in the various methods and compositions discloses herein include glyphosate, ACCase inhibitors (Arloxyphenoxy propionate (FOPS)); ALS inhibitors (Sulfonylurea (SU)), Imidazonlinone (IMI), Pyrimidines (PM)); microtubule protein inhibitor (Dinitroaniline (DNA)); synthetic auxins (Phenoxy (P)), Benzoic Acid (BA), Carboxylic acid (CA)); Photosystem II inhibitor (Triazine (TZ)), Triazinone (TN), Nitriles (NT), Benzothiadiazinones (BZ), Ureas (US)); EPSP Synthase inhibitor (glycines (GC)); Glutamine Synthesis inhibitor (Phosphinic Acid (PA)); DOXP synthase inhibitor (Isoxazolidinone (IA)); HPPD inhibitor (Pyrazole (PA)), Triketone (TE)); PPO inhibitors (Diphenylether (DE), N- phenylphthalimide (NP) (Ary triazinone (AT)); VLFA inhibitors (chloroacetamide (CA)), Oxyacetamide (OA)); Photosystem I inhibitor (Bipyridyliums (BP)); and the like.

Pesticides that can be used in the various methods and compositions disclosed herein include imidacloprid clothianidin, arylpyrazole compounds (W02007103076); organophosphates, phenyl pyrazole, pyrethoids caramoyloximes, pyrazoles, amidines, halogenated hydrocarbons, carbamates and derivatives thereof, terbufos, chloropyrifos, fipronil, chlorethoxyfos, telfuthrin, carbofuran, imidacloprid, tebupirimfos (U.S. Patent No. 5,849,320).

Nematicides that can be used in the various methods and compositions disclosed herein include any synthetic nematicide or biological nematicide. Examples of synthetic nematicides include, but are not limited to, acibenzolar-S-methyl, an avermectin (e.g., abamectin), carbamate nematicides (e.g., aldicarb, thiadicarb, carbofuran, carbosulfan, oxamyl, aldoxycarb, ethoprop, methomyl, benomyl, alanycarb), organophosphorus nematicides (e.g., phenamiphos (fenamiphos), fensulfothion, terbufos, fosthiazate, dimethoate, phosphocarb, dichlofenthion, isamidofos, fosthietan, isazofos ethoprophos, cadusafos, terbufos, chlorpyrifos, dichlofenthion, heterophos, isamidofos, mecarphon, phorate, thionazin, triazophos, diamidafos, fosthietan, phosphamidon), and certain fungicides, such as captan, thiophanate-methyl and thiabendazole. Biological nematicides include, but are not limited to, nematicides include ARF18; Arthrobotrys spp.; Chaetomium spp.; Cylindrocarpon spp.; Exophilia spp.; Fusarium spp.; Gliocladium spp.; Hirsutella spp.; Lecanicillium spp . : Monacrosporium spp.;Myrothecium spp.; Neocosmospora spp.; Paecilomyces spp.; Pochonia spp.; Stagonospora spp.; vesicular-arbuscular mycorrhizal fungi, Burkholderia spp.; Pasteuria spp., Brevibacillus spp.; Pseudomonas spp.; Rhizobacteria; and Bacillus spp.

Fungicides and/or bactericides that can be used in the various methods and compositions disclosed herein include aliphatic nitrogen fungicides (butylamine, cymoxanil, dodicin, dodine, guazatine, iminoctadine); amide fungicides (benzovindiflupyr, carpropamid, chloraniformethan, cyflufenamid, diclocymet, diclocymet, dimoxystrobin, fenaminstrobin, fenoxanil, flumetover, furametpyr, isofetamid, isopyrazam, mandestrobin, mandipropamid, metominostrobin, orysastrobin, penthiopyrad, prochloraz, quinazamid, silthiofam, triforine); acylamino acid fungicides (benalaxyl, benalaxyl-M, furalaxyl, metalaxyl, metalaxyl-M, pefurazoate, valifenalate); anilide fungicides (benalaxyl, benalaxyl-M, bixafen, boscalid, carboxin, fenhexamid, fluxapyroxad, isotianil, metalaxyl, metalaxyl-M, metsulfovax, ofurace, oxadixyl, oxycarboxin, penflufen, pyracarbolid, sedaxane, thifluzamide, tiadinil, vanguard); benzanilide fungicides (benodanil, flutolanil, mebenil, mepronil, salicylanilide, tecloftalam); furanilide fungicides (fenfuram, furalaxyl, furcarbanil, methfuroxam); sulfonanilide fungicides (flusulfamide); benzamide fungicides (benzohydroxamic acid, fluopicolide, fluopyram, tioxymid, trichlamide, zarilamid, zoxamide); furamide fungicides (cyclafuramid, furmecyclox); phenylsulfamide fungicides (dichlofluanid, tolylfluanid); sulfonamide fungicides (amisulbrom, cyazofamid); valinamide fungicides (benthiavalicarb, iprovalicarb); antibiotic fungicides (aureofungin, blasticidin-S, cycloheximide, griseofulvin, kasugamycin, moroxydine, natamycin, polyoxins, polyoxorim, streptomycin, validamycin); strobilurin fungicides (fluoxastrobin, mandestrobin); methoxyacrylate strobilurin fungicides (azoxystrobin, bifujunzhi, coumoxystrobin, enoxastrobin, flufcnoxystrobin. jiaxiangjunzhi. picoxystrobin, pyraoxystrobin); methoxycarbanilate strobilurin fungicides (pyraclostrobin, pyrametostrobin, triclopyricarb); methoxyiminoacetamide strobilurin fungicides (dimoxystrobin, fenaminstrobin, metominostrobin, orysastrobin); methoxyiminoacetate strobilurin fungicides (kresoxim-methyl, trifloxystrobin); aromatic fungicides (biphenyl, chlorodinitronaphthalenes, chloroneb, chlorothalonil, cresol, dicloran, fenjuntong, hexachlorobenzene, pentachlorophenol, quintozene, sodium pentachlorophenoxide, tecnazene, trichlorotrinitrobenzenes); arsenical fungicides (asomate, urbacide); aryl phenyl ketone fungicides (metrafenone, pyriofenone); benzimidazole fungicides (albendazole, benomyl, carbendazim, chlorfenazole, cypendazole, debacarb, fuberidazole, mecarbinzid, rabenzazole, thiabendazole); benzimidazole precursor fungicides (furophanate, thiophanate, thiophanate -methyl); benzothiazole fungicides (bentaluron, benthiavalicarb, benthiazole, chlobenthiazone, probenazole); botanical fungicides (allicin, berberine, carvacrol, carvone, osthol, sanguinarine, santonin); bridged diphenyl fungicides (bithionol, dichlorophen, diphenylamine, hexachlorophene, parinol); carbamate fungicides (benthiavalicarb, furophanate, iodocarb, iprovalicarb, picarbutrazox, propamocarb, pyribencarb, thiophanate, thiophanate-methyl, tolprocarb); benzimidazolylcarbamate fungicides (albendazole, benomyl, carbendazim, cypendazole, debacarb, mecarbinzid); carbanilate fungicides (diethofencarb, pyraclostrobin, pyrametostrobin, triclopyricarb); conazole fungicides, conazole fungicides (imidazoles) (climbazole, clotrimazole, imazalil, oxpoconazole, prochloraz, triflumizole); conazole fungicides (triazoles) (azaconazole, bromuconazole, cyproconazole, diclobutrazol, difenoconazole, diniconazole, diniconazole-M, epoxiconazole, etaconazole, fenbuconazole, fluquinconazole, flusilazole, flutriafol, furconazole, furconazole-cis, hexaconazole, imibenconazole, ipconazole, metconazole, myclobutanil, penconazole, propiconazole, prothioconazole, quinconazole, simeconazole, tebuconazole, tetraconazole, triadimefon, triadimenol, triticonazole, uniconazole, uniconazole-P); copper fungicides (acypetacs-copper, Bordeaux mixture, Burgundy mixture, Cheshunt mixture, copper acetate, basic copper carbonate, copper hydroxide, copper linoleate, copper naphthenate, a copper compound such as copper oleate, copper oxychloride, copper octanoate, copper silicate, copper sulfate, copper sulfate pentahydrate, tribasic copper sulfate, or copper zinc chromate; cufraneb, cuprobam, cuprous oxide, mancopper, oxine-copper, saisentong, thiodiazole-copper); cyanoacrylate fungicides (benzamacril, phenamacril); dicarboximide fungicides (famoxadone, fluoroimide); dichlorophenyl dicarboximide fungicides (chlozolinate, dichlozoline, iprodione, isovaledione, myclozolin, procymidone, vinclozolin); phthalimide fungicides (captafol, captan, ditalimfos, folpet, thiochlorfenphim); dinitrophenol fungicides (binapacryl, dinobuton, dinocap, dinocap-4, dinocap-6, meptyldinocap, dinocton, dinopenton, dinosulfon, dinoterbon, DNOC); dithiocarbamate fungicides (amobam, asomate, azithiram, carbamorph, cufraneb, cuprobam, disulfiram, ferbam, metam, nabam, tecoram, thiram, urbacide, ziram); cyclic dithiocarbamate fungicides (dazomet, etem, milneb); polymeric dithiocarbamate fungicides (mancopper, mancozeb, maneb, metiram, polycarbamate, propineb, zineb); dithiolane fungicides (isoprothiolane, saijunmao); fumigant fungicides (carbon disulfide, cyanogen, dithioether, methyl bromide, methyl iodide, sodium tetrathiocarbonate); hydrazide fungicides (benquinox, saijunmao); imidazole fungicides (cyazofamid, fenamidone, fenapanil, glyodin, iprodione, isovaledione, pefurazoate, triazoxide); conazole fungicides (imidazoles) (climbazole, clotrimazole, imazalil, oxpoconazole, prochloraz, triflumizole); inorganic fungicides (potassium azide, potassium thiocyanate, sodium azide, sulfur, see also copper fungicides, see also inorganic mercury fungicides); mercury fungicides; inorganic mercury fungicides (mercuric chloride, mercuric oxide, mercurous chloride); organomercury fungicides ((3- ethoxypropyl)mercury bromide, ethylmercury acetate, ethylmercury bromide, ethylmercury chloride, ethylmercury 2,3-dihydroxypropyl mercaptide, ethylmercury phosphate, /V-(ethylmercury)-/>- toluenesulphonanilide, hydrargaphen, 2-methoxyethylmercury chloride, methylmercury benzoate, methylmercury dicyandiamide, methylmercury pentachlorophenoxide, 8-phenylmercurioxyquinoline, phenylmercuriurea, phenylmercury acetate, phenylmercury chloride, phenylmercury derivative of pyrocatechol, phenylmercury nitrate, phenylmercury salicylate, thiomersal, tolylmercury acetate); morpholine fungicides (aldimorph, benzamorf, carbamorph, dimethomorph, dodemorph, fenpropimorph, flumorph, tridemorph); organophosphorus fungicides (ampropylfos, ditalimfos, EBP, edifenphos, fosetyl, hexylthiofos, inezin, iprobenfos, izopamfos, kejunlin, phosdiphen, pyrazophos, tolclofos-methyl, triamiphos); organotin fungicides (decafentin, fentin, tributyltin oxide); oxathiin fungicides (carboxin, oxycarboxin); oxazole fungicides (chlozolinate, dichlozoline, drazoxolon, famoxadone, hymexazol, metazoxolon, myclozolin, oxadixyl, oxathiapiprolin, pyrisoxazole, vinclozolin); polysulfide fungicides (barium polysulfide, calcium polysulfide, potassium polysulfide, sodium polysulfide); pyrazole fungicides (benzovindiflupyr, bixafen, fenpyrazamine, fluxapyroxad, furametpyr, isopyrazam, oxathiapiprolin, penflufen, penthiopyrad, pyraclostrobin, pyrametostrobin, pyraoxystrobin, rabenzazole, sedaxane); pyridine fungicides (boscalid, buthiobate, dipyrithione, fluazinam, fluopicolide, fluopyram, parinol, picarbutrazox, pyribencarb, pyridinitril, pyrifenox, pyrisoxazole, pyroxychlor, pyroxyfur, triclopyricarb); pyrimidine fungicides (bupirimate, diflumetorim, dimethirimol, ethirimol, fenarimol, ferimzone, nuarimol, triarimol); anilinopyrimidine fungicides (cyprodinil, mepanipyrim, pyrimethanil); pyrrole fungicides (dimetachlone, fenpiclonil, fludioxonil, fluoroimide); quaternary ammonium fungicides (berberine, sanguinarine); quinoline fungicides (ethoxyquin, halacrinate, 8 -hydroxy quinoline sulfate, quinacetol, quinoxyfen, tebufloquin); quinone fungicides (chloranil, dichlone, dithianon); quinoxaline fungicides (chinomethionat, chlorquinox, thioquinox); thiadiazole fungicides (etridiazole, saisentong, thiodiazole-copper, zinc thiazole); thiazole fungicides (ethaboxam, isotianil, metsulfovax, octhilinone, oxathiapiprolin, thiabendazole, thifluzamide); thiazolidine fungicides (flutianil, thiadifluor); thiocarbamate fungicides (methasulfocarb, prothiocarb); thiophene fungicides (ethaboxam, isofetamid, silthiofam); triazine fungicides (anilazine); triazole fungicides (amisulbrom, bitertanol, fluotrimazole, triazbutil); conazole fungicides (triazoles) (azaconazole, bromuconazole, cyproconazole, diclobutrazol, difenoconazole, diniconazole, diniconazole-M, epoxiconazole, etaconazole, fenbuconazole, fluquinconazole, flusilazole, flutriafol, furconazole, furconazole-cis, hexaconazole, huanjunzuo, imibenconazole, ipconazole, metconazole, myclobutanil, penconazole, propiconazole, prothioconazole, quinconazole, simeconazole, tebuconazole, tetraconazole, triadimefon, triadimenol, triticonazole, uniconazole, uniconazole-P); triazolopyrimidine fungicides (ametoctradin); urea fungicides (bentaluron, pencycuron, quinazamid); zinc fungicides (acypetacs-zinc, copper zinc chromate, cufraneb, mancozeb, metiram, polycarbamate, polyoxorim-zinc, propineb, zinc naphthenate, zinc thiazole, zinc trichlorophenoxide, zineb, ziram); unclassified fungicides (acibenzolar, acypetacs, allyl alcohol, benzalkonium chloride, bethoxazin, bromothalonil, chitosan, chloropicrin, DBCP, dehydroacetic acid, diclomezine, diethyl pyrocarbonate, ethylicin, fenaminosulf, fenitropan, fenpropidin, formaldehyde, furfural, hexachlorobutadiene, methyl isothiocyanate, nitrostyrene, nitrothal-isopropyl, OCH, pentachlorophenyl laurate, 2-phenylphenol, phthalide, piperalin, propamidine, proquinazid, pyroquilon, sodium orthophenylphenoxide, spiroxamine, sultropen, thicyofen, tricyclazole), or mefenoxam.

In some embodiments, the biocide (for example a herbicide, bactericide, fungicide, pesticide, and/or insecticide) is a biocontrol agent. In such instances, the bacterial strain provided herein or active variant therof, and/or a composition derived therefrom is compatible with the biocontrol agent of interest.

Biocontrol agents that can be used in the various methods and compositions disclosed herein include Bacillus amyloliquefaciens strain D747, (e.g. DOUBLENICKEL™ 55 or DOUBLENICKEL™ LC from Certis USA, L.L.C, having Accession No. FERM BP-8234 and described in U.S. Patent No. 7,094,592, incorporated by reference in its entirety herein); Bacillus subtilis strain QST 713/AQ713 (e.g.

SERENADE® MAX from Bayer CropScience LP, US, having NRRL Accession No. B-21661 and described in U.S. Patent No. 6,060,051, incorporated by reference in its entirety herein; Bacillus subtilis strain AQ30002 (aka QST30002; NRRL Accession No. B-50421), and AQ30004 (aka QST3004; NRRL Accession No. B-50455), both from Bayer CropScience LP, US and described in U.S. Patent No. 9,185,915, incorporated by reference in its entirety herein; Bacillus subtilis strain QST 713 (e.g. RHAPSODY® from Bayer CropScience, LP, US); Bacillus amyloliquefaciens strain GB03 (e.g. KODIAK® from Bayer CropScience, AG, DE); Bacillus subtilis strain FB17 (e.g. Veolondis™ from BASF); Bacillus pumilus strain QST 2808 (e.g. SONATA® from Bayer CropScience, having NRRF Accession No. B-30087); Bacillus pumilus strain GB34 (e.g. YIEFD SHIEFD from Bayer CropScience AG, DE); Bacillus subtilis var. amyloliquefaciens strain FZB24 (e.g. Taegro® from Syngenta); Bacillus finnus strain CNMC 1-1582 (e.g. VOTiVO® from Bayer CropScience); Streptomyces lydicus strain WYEC108 (e.g. ACTINOVATE® from Natural Industries, US, having ATCC Accession No. 55445): Streptomyces griseoviridis strain K61 (e.g. MYCOSTOP® from Verdera, cf. Crop Protection 2006, 25, 468-475, having Accession No. DSM 7206); Agrobacterium radiobacter strain 1026 (e.g. NOGALL™ from Becker Underwood, US); Agrobacterium radiobacter strain K84 (e.g. GALLTROL-A® from AgBioChem, CA); Pseudomonas fluorescens strain A506 (e.g. BLIGHTBAN® by NuFarm and also e.g. FROSTBAN B by Frost Technology Corp); Bacillus thuringiensis subspecies aizawai strain GC-91 (e.g. AGREE® from Certis USA, LLC); Bacillus thuringiensis subspecies kurstaki (e.g. BT 320 DUST from Wilbur-Ellis Company); Bacillus thuringiensis subspecies kurstaki strain EG7841 (e.g. CRYMAX® from Certis USA LLC); Bacillus thuringiensis subspecies kurstaki strain SA-12 (e.g. DELIVER® from Certis USA LLC); Bacillus thuringiensis subspecies kurstaki strain ABTS-351 (e.g. DiPel® from Valent BioSciences Corp, having ATCC Accession No. SD-1275); Bacillus thuringiensis subspecies kurstaki strain SA-11 (e.g. JAVELIN® from Certis USA LLC); Bacillus thuringiensis subspecies tenebrionis strain SA-10 (e.g. TRIDENT® from Certis USA LLC); Chromobacterium subtsugae strain PRAA4-1 (e.g. GRANDEVO® from Marrone Bioinnovations, USA); Isaria fumosorosea Apopka Strain 97 (e.g. PFR-97™ from Certis USA LLC, having ATTC Accssion No. 20874); Burkholderia spp. strain A396 (e.g. VENERATE™ from Marrone Bioinnovations, USA); Bacillus thuringiensis subspecies aizawai strain ABTS-1857 (e.g. XENTARI® from Valent BioSciences Corp,

USA); a biologically pure strain of Pseudomonas fluorescens selected from ATCC 55171, ATCC 55170, ATCC 55169, ATCC 55175, ATCC 55174, and ATCC 55168 as described in U.S. Patent 5,348,742, incorporated by reference herein; AIP001620 and AIP050999 as described in WO 2015/116838, incorporated by reference herein; AIP27511, AIP35174, AIP25773, AIP15251, AIP61892, AIP79428, AIP14931, AIP39589, and AIP36895 as described in WO 2017/040273, incorporated by reference herein; AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, and AIP085784 as described in WO 2019/023226, incorporated by reference herein; AIP000648, AIP097852, and AIP051459 as described in WO 2019/074813, incorporated by reference herein; AIP031898, AIP023234, AIP024552, AIP035573, AIP071234, AIP080021, AIP001237, AIP050674, AIP071546, AIP049805, AIP016229, AIP081435, AIP082140, AIP000817, AIP060333, AIP070494, AIP015104, AIP011586, AIP010884, AIP082287, AIP088026, AIP065969, AIP018514, AIP033041, AIP092281, AIP081114, AIP046403, AIP022635, AIP070925, and AIP039063 as described in WO 2020/006555, incorporated by reference herein; AIP075655, AIP061382, and AIP029105 as described in WO 2020/077042, incorporated by reference herein; and AIP045885, AIP075655, AIP09474, AIP024525, AIP033287, AIP093798, AIP061639, AIP082862, AIP058187, AIP059286, and AIP036706 as described in WO 2020/092381, incorporated by reference herein.

In some embodiments, biocontrol agents that can be used in the various methods and compositions disclosed herein are pesticidal fungal strains. These fungal strains of interest include Trichoderma harzianum strain KRL-AG2 (also known as strain T-22, e g. PLANTSHIELD® T-22G, ROOTSHIELD®, and TURFSHIELD from BioWorks, USA, having ATCC Accession No. 408479); Gliochladium vierns, aka Trichoderma virens, strain GL-21 (e.g. SOILGARD® 12G from Certis USA, L.L.C); Coniothyrium minitans strain CON/M/91-8 (e.g. CONTANS® from Encore Technologies, LLC, having Accession No. DSM-9660): Purpureocilium lilacinum; Ulocladium oudemansii U3 strain (akaHRU3 strain), (e.g. BOTRY-ZEN® by Botry-Zen Ltd, NZ); and Beauveria hassiana strain GHA (e.g. MYCOTROL® from Lam International Corp.).

In some embodiments, biocontrol agents that can be used in the various methods and compositions disclosed herein are pesticidal virus isolates. A virus may refer to a complete viral isolate itself or may refer to viral occlusion bodies. These viruses of interest include Cydia pomonella granulovirus (e.g. CYD-X® and CYD-X® HP, both from Certis USA LLC; CARPOVIRUSINE® from Sumitomo Corp.); Cydia pomonella granulovirus isolate V22 (e.g. MADEX HP from Andermatt Biocontrol AG); and polyhedral occlusion bodies of the nuclear polyhedrosis virus of Helicoverpa zea (e.g. GEMSTAR® from Certis USA LLC).

In some embodiments, biocontrol agents that can be used in the various methods and compositions disclosed herein are biopesticides derived from plant extracts. These biopesticides of interest include extracts of Chenopodium ambrosioides (e.g. Requiem® from Bayer CropScience LP).

In some embodiments, biocontrol agents that can be used in the various methods and compositions disclosed herein are biostimulants. A biostimulant is any microorganism or substance based on nautral resources. It is applied to plants, seeds, or the rhizosphere with the intention to stimulate natural processes of plants to benefit nutrient uptake, nutrient use efficiency, tolerance to abiotic stresses, and/or general plant health, including resistance to disease. Biostimulants include plant extracts, such as for example extracts derived from Reynoutria sachalinensis (e.g. REGALIA® from Marrone Bioinnovations, USA).

In some embodiments of the invention, a kit of parts is provided comprising a bacterial strain provided herein or active variant thereof, and/or a composition derived therefrom, and at least one biocide, in a spatially separated arrangement. In some embodiments, the biocide is an herbicide, fungicide, insecticide, bactericide, nematicide, pesticide, or other crop protection chemical.

Non-limiting embodiments of the invention include:

1. A formulation comprising a biocontrol agent, wherein the biocontrol agent comprises: (a) abacterial strain deposited as NRRL No. B-67754, NRRL No. B-67651, orNRRLNo. B- 67653; or

(b) a supernatant, fermentation product, filtrate, or extract derived from a whole cell culture of a bacterial strain deposited as NRRL No. B-67754, NRRL No. B-67651, or NRRL No. B-67653.

2. The formulation of embodiment 1, wherein the formulation is a dry formulation or a liquid formulation.

3. The formulation of embodiment 2, wherein said dry formulation is dried to a water activity of 0.3 or less.

4. The formulation of any one of embodiments 1-3, wherein the formulation is a spray dried formulation, a wettable powder, or a granule.

5. The formulation of any one of embodiments 1-4, wherein the biocontrol agent is present at about 10 ⁵ CFU/gram to about 10 ¹² CFU/gram or at about 10 ⁵ CFU/ml to about 10 ¹² CFU/ml.

6. The formulation of any one of embodiments 1-5, wherein the formulation further comprises a pesticide, a fungicide, a bactericide, a nematicide, an insecticide, or an herbicide.

7. The formulation of embodiment 6, wherein the fungicide or bactericide comprises prothioconazole, azoxystrobin, fluopicolide, chlorothalonil, fosetyl, fenhexamid, flutriafol, difenoconazole, tebuconazole, tetraconazole, pyraclostrobin, trifloxystrobin, propiconazole, fluoxastrobin, flutolanil, metconazole, a copper compound, or metrafenone.

8. The formulation of any one of embodiments 1-7, wherein the formulation comprises a second biocontrol agent.

9. The formulation of embodiment 8, wherein the second biocontrol agent comprises Bacillus amyloliquefaciens strain D747, Bacillus subtilis strain QST 713, Bacillus amyloliquefaciens strain GB03, Bacillus subtilis strain FBI 7, Bacillus pumilus strain QST 2808, Bacillus pumilus strain GB34, Bacillus subtilis var. amyloliquefaciens strain FZB24, Bacillus finnus strain CNMC 1-1582, Streptomyces lydicus strain WYEC108, Streptomyces griseoviridis strain K61, Agrobacterium radiobacter strain 1026, Agrobacterium radiobacter strain K84, Pseudomonas fluorescens strain A506, Bacillus thuringiensis subspecies aizawai strain GC-91, Bacillus thuringiensis subspecies kurstaki, Bacillus thuringiensis subspecies kurstaki strain EG7841, Bacillus thuringiensis subspecies kurstaki strain SA-12, Bacillus thuringiensis subspecies kurstaki strain ABTS-351, Bacillus thuringiensis subspecies kurstaki strain SA-11, Bacillus thuringiensis subspecies tenebrionis strain SA-10, Chromobacterium subtsugae strain PRAA4-1, Isaria fumosorosea Apopka Strain 97, Burkholderia spp. strain A396, Bacillus thuringiensis subspecies aizawai strain ABTS-1857, Pseudomonas fluorescens strains AIP0069 or AIP1620, or Bacillus subtilis subsp. subtilis strain AIP61892.

10. The formulation of any one of embodiments 1-9, wherein said formulation is a stable formulation.

11. A coated seed comprising a seed and a coating on the seed, wherein the coating comprises a formulation comprising a biocontrol agent, wherein the biocontrol agent comprises a bacterial strain deposited as NRRL No. B-67754, NRRL No. B-67651, orNRRL No. B-67653.

12. The coated seed of embodiment 11, wherein the biocontrol agent is present at about 10 ⁵ CFU/gram to about 10 ¹² CFU/gram or at about 10 ⁵ CFU/ml to about 10 ¹² CFU/ml.

13. The coated seed of embodiment 11 or 12, wherein the coating further comprises a pesticide, a fungicide, a bactericide, a nematicide, an insecticide or an herbicide.

14. The coated seed of embodiment 13, wherein the fungicide comprises prothioconazole, azoxystrobin, fluopicolide, chlorothalonil, fosetyl, fenhexamid, flutriafol, difenoconazole, tebuconazole, tetraconazole, pyraclostrobin, trifloxystrobin, propiconazole, fluoxastrobin, flutolanil, metconazole, a copper compound, or metrafenone.

15. The coated seed of any one of embodiments 11-14, wherein the coating comprises a second biocontrol agent.

16. The coated seed of embodiment 15, wherein the second biocontrol agent comprises Bacillus amyloliquefaciens strain D747, Bacillus subtilis strain QST 713, Bacillus amyloliquefaciens strain GB03, Bacillus subtilis strain FBI 7, Bacillus pumilus strain QST 2808, Bacillus pumilus strain GB34, Bacillus subtilis var. amyloliquefaciens strain FZB24, Bacillus finnus strain CNMC 1-1582, Streptomyces lydicus strain WYEC108, Streptomyces griseoviridis strain K61, Agrobacterium radiobacter strain 1026, Agrobacterium radiobacter strain K84, Pseudomonas fluorescens strain A506, Bacillus thuringiensis subspecies aizawai strain GC-91, Bacillus thuringiensis subspecies kurstaki, Bacillus thuringiensis subspecies kurstaki strain EG7841, Bacillus thuringiensis subspecies kurstaki strain SA-12, Bacillus thuringiensis subspecies kurstaki strain ABTS-351, Bacillus thuringiensis subspecies kurstaki strain SA-11, Bacillus thuringiensis subspecies tenebrionis strain SA-10, Chromobacterium subtsugae strain PRAA4-1, Isaria fumosorosea Apopka Strain 97, Burkholderia spp. strain A396, Bacillus thuringiensis subspecies aizawai strain ABTS-1857, Pseudomonas fluorescens strains AIP0069 or AIP1620, ox Bacillus subtilis subsp. subtilis strain AIP61892.

17. A composition comprising an effective amount of a biocontrol agent, wherein the biocontrol agent comprises:

(a) a bacterial strain deposited as NRRL No. B-67754, NRRL No. B-67651, or NRRL No.

B-67653; or

(b) a supernatant, fermentation product, filtrate, or extract derived from a whole cell culture of a bacterial strain deposited as NRRL No. B-67754, NRRL No. B-67651, or NRRL No. B-67653; wherein said effective amount of said composition controls a plant pest.

18. The composition of embodiment 17, wherein the bacterial strain is present in about 10 ⁵ CFU/gram to about 10 ¹² CFU/gram or in about 10 ⁵ CFU/ml to about 10 ¹² CFU/ml.

19. The composition of embodiment 17 or 18, wherein the composition further comprises a pesticide, a fungicide, a bactericide, a nematicide, an insecticide, or an herbicide.

20. The composition of embodiment 19, wherein the fungicide or bactericide comprises prothioconazole, azoxystrobin, fluopicolide, chlorothalonil, fosetyl, fenhexamid, flutriafol, difenoconazole, tebuconazole, tetraconazole, pyraclostrobin, trifloxystrobin, propiconazole, fluoxastrobin, flutolanil, metconazole, a copper compound, or metrafenone.

21. The composition of any one of embodiments 17-20, wherein the coating comprises a second biocontrol agent.

22. The composition of embodiment 21, wherein the second biocontrol agent comprises Bacillus amyloliquefaciens strain D747, Bacillus subtilis strain QST 713, Bacillus amyloliquefaciens strain GB03, Bacillus subtilis strain FBI 7, Bacillus pumilus strain QST 2808, Bacillus pumilus strain GB34, Bacillus subtilis var. amyloliquefaciens strain FZB24, Bacillus finnus strain CNMC 1-1582, Streptomyces lydicus strain WYEC108, Streptomyces griseoviridis strain K61, Agrobacterium radiobacter strain 1026, Agrobacterium radiobacter strain K84, Pseudomonas fluorescens strain A506, Bacillus thuringiensis subspecies aizawai strain GC-91, Bacillus thuringiensis subspecies kurstaki, Bacillus thuringiensis subspecies kurstaki strain EG7841, Bacillus thuringiensis subspecies kurstaki strain SA-12, Bacillus thuringiensis subspecies kurstaki strain ABTS-351, Bacillus thuringiensis subspecies kurstaki strain SA-11, Bacillus thuringiensis subspecies tenebrionis strain SA-10, Chromobacterium subtsugae strain PRAA4-1, Isaria fumosorosea Apopka Strain 97, Burkholderia spp. strain A396, Bacillus thuringiensis subspecies aizawai strain ABTS-1857, Pseudomonas fluorescens strains AIP0069 or AIP1620, ox Bacillus subtilis subsp. subtilis strain AIP61892.

23. A method for controlling a plant pest comprising contacting said pest with an effective amount of the composition of any one of embodiments 17-22, or the formulation of any one of embodiments 1-9, wherein said composition or said formulation controls said plant pest.

24. The method of embodiment 23, wherein the plant pest is an insect, nematode, fungus, fungal-like organism, virus, viroid, bacterium, or a protozoan pathogen.

25. The method of embodiment 23 or 24, wherein said plant pest comprises one or more fungal pathogens and/or fungal -like pathogens selected from the group consisting of Aspergillus flavus, Aspergillus spp., Albugo occidentalis, Albugo spp., Alternaria solani, Alternaria spp., Apiognomonia errabunda, Apiognomonia veneta, Apiognomonia spp., Armillaria mellea, Armillaria spp., Bipolaris maydis, Botrytis cinerea, Botrytis squamosa, Botrytis spp., Botryosphaeria dothidea, Botryosphaeira spp., Blumeriella jaapii, Blumeriella spp., Bremia lactucae, Bremia spp., Cladosporium carpophilum, Cladosporium caryigenum, Cladosporium spp., Colletotrichum acutatum, Colletotrichum graminicola, Colletotrichum cereale, Colletotrichum gloeosporiodes, Colletotrichum sublineolum, Colletotrichum spp, Cochliobolus heterostrophus, Corynespora cassiicola, Corynespora spp., Discula fraxinea, Cercospora sojina, Cercospora beticola, Cercospora spp., Blumeria graminis f. sp. Tritici, Didymella bryoniae, Didymella spp., Elsinoe fawcetti, Elsinoe spp., Erysiphe necator, Erysiphe lager stroemiae, Erysiphe cichoracearum, Erysiphe spp., Eutypa lata, Eutypa spp., Fusarium graminearum, Fusarium solani, Fusarium oxysporum, Fusarium graminicola, Fusarium spp., Golovinomyces cichoracearum, Gibberella zeae, Gibberella spp., Gloeodes pomigena, Gymnosporangium juniperi-virginianae, Hemileia vastatrix, Leveillula taurica, Leveillula Mycosphaerella fijiensis, Mycosphaerella citri, Mycosphaerella pomi, Mycosphaerella spp.,

Macrophomina spp., Monosporas cits cannonballus, Monosporascus spp.. Monilinia fructicola, Monilinia laxa, Monilinia fructigena, Monilinia spp., Neofabraea spp., Podosphaera xanthii, Podosphaera leucotricha, Podosphaera spp., Phomopsis viticola, Phomopsis spp., Penicillium spp., Phakopsora meibomiae, Phakopsora pachyrizi, Phakopsora spp., Puccinia triticina, Puccinia recondita, Puccinia striiformis, Puccinia graminis, Puccinia spp., Pyrenophora trici-repentis , Rhizoctonia solani, Rhizoctonia spp., Rhizopus spp., Ramularia spp., Tilletia barclayena, Tilletia spp., Uncinula necator, Uncinula spp,. Uromyces betae, Uromyces spp., Phoma spp., Sclerotium rolfsii, Sclerotium spp., Sclerotinia minor, Sclerotinia sclerotiorium, Sclerotinia spp., Schizothyrium pomi, Schizothyrium spp., Septoria glycines, Septoria spp., Sphaerotheca pannosa, Sphaerotheca macularis, Sphaerotheca spp., Sphaceloma spp., Venturia inaequalis, Venturia spp., Verticillium spp., Wilsonomyces carpophilus, Wilsonomyces spp., Pythium cryptoirregulare, Pythium aphanidermatum, Pythium irregulare, Pythium sylvaticum, Pythium myriotylum, Pythium ultimum, Pythium spp., Phytophthora capsid, Phytophthora nicotianae, Phytophthora infestans, Phytophthora tropicalis, Phytophthora sojae, Phytophthora spp., Peronospora belbahrii, Peronospora lamii, Peronospora farinosa, Peronospora spp., Pseudoperonospora cubensis, Pseudoperonospora spp., Bremia spp., Plasmopara viticola, Plasmopara obduscens, Plasmopara spp., Basidiophora spp., Plasmodiophora brassicae, and P las modi ophora spp.

26. The method of embodiment 24, wherein said fungal and/or fungal -like pathogen is a Colletotrichum spp., Phytophthora spp., Podosphaera spp., Aspergillus spp.,Mycosphaerella. spp., Uncinula spp., or Botrytis spp.

27. The method of embodiment 26, wherein said fungal and/or fungal -like pathogen is Colletotrichum sublineolum, Phytophthora infestans, Podosphaera xanthii, Aspergillus flavus, Mycosphaerella fijiensis, Uncinula necator, or Botrytis cinera.

28. The method of embodiment 23 or 24, wherein said plant pest comprises one or more bacterial pathogens selected from the group consisting of Acidovorax avenae, Burkholderia gladioli, Candidatus Liberibacter spp., Erwinia amylovora, Erwinia ananas, Erwinia chrysanthemi, Erwinia dissolvens, Erwinia herbicola, Erwinia rhapontic, Erwinia stewartiii, Erwinia tracheiphila, Erwinia uredovora; Pectobacterium carotovorum, Pseudomonas syringae, Streptomyces scabies, Xanthomonas campestris, Xanthomonas axonopodis, Xanthomonas fragariae; Xanthomonas translucens, and Xylella fastidiosa.

29. The method of any one of embodiments 23-28, wherein a second biocontrol agent is applied.

30. The method of embodiment 29, wherein the second biocontrol agent comprises Bacillus amyloliquefaciens strain D747, Bacillus subtilis strain QST 713, Bacillus amyloliquefaciens strain GB03, Bacillus subtilis strain FBI 7, Bacillus pumilus strain QST 2808, Bacillus pumilus strain GB34, Bacillus subtilis var. amyloliquefaciens strain FZB24, Bacillus fmnus strain CNMC 1-1582, Streptomyces lydicus strain WYEC108, Streptomyces griseoviridis strain K61, Agrobacterium radiobacter strain 1026, Agrobacterium radiobacter strain K84, Pseudomonas fluorescens strain A506, Bacillus thuringiensis subspecies aizawai strain GC-91, Bacillus thuringiensis subspecies kurstaki, Bacillus thuringiensis subspecies kurstaki strain EG7841, Bacillus thuringiensis subspecies kurstaki strain SA-12, Bacillus thuringiensis subspecies kurstaki strain ABTS-351, Bacillus thuringiensis subspecies kurstaki strain SA-11, Bacillus thuringiensis subspecies tenebrionis strain SA-10, Chromobacterium subtsugae strain PRAA4-1, Isaria fumosorosea Apopka Strain 97, Burkholderia spp. strain A396, Bacillus thuringiensis subspecies aizawai strain ABTS-1857, Pseudomonas fluorescens strains AIP0069 or AIP1620, or Bacillus subtilis subsp. subtilis strain AIP61892. 31. The method of embodiment 29 or 30, wherein the biocontrol agent and the second biocontrol agent are applied simultaneously.

32. The method of embodiment 29 or 30, wherein the biocontrol agent and the second biocontrol agent are applied sequentially.

33. A method for growing a plant susceptible to a plant pest comprising applying to a plant, a plant part, a seed, or an area of cultivation a biocontrol agent, wherein the biocontrol agent comprises:

(a) an effective amount of a bacterial strain deposited as NRRL No. B-67754, NRRL No. B- 67651, or NRRL No. B-67653; or

(b) an effective amount of a supernatant, fermentation product, fdtrate, or extract derived from a whole cell culture of a bacterial strain deposited as NRRL No. B-67754, NRRL No. B-67651, or NRRL No. B-67653; wherein said effective amount controls a plant pest.

34. The method of embodiment 33, wherein said effective amount of the bacterial strain comprises at least about 10 ⁴ to 10 ¹⁶ colony forming units (CFU) per hectare or at least about 10 ⁶ to 10 ¹⁸ cells per hectare.

35. The method of embodiment 33 or 34, wherein a second biocontrol agent is applied.

36. The method of embodiment 35, wherein the second biocontrol agent comprises Bacillus amyloliquefaciens strain D747, Bacillus subtilis strain QST 713, Bacillus amyloliquefaciens strain GB03, Bacillus subtilis strain FBI 7, Bacillus pumilus strain QST 2808, Bacillus pumilus strain GB34, Bacillus subtilis var. amyloliquefaciens strain FZB24, Bacillus finnus strain CNMC 1-1582, Streptomyces lydicus strain WYEC108, Streptomyces griseoviridis strain K61, Agrobacterium radiobacter strain 1026, Agrobacterium radiobacter strain K84, Pseudomonas fluorescens strain A506, Bacillus thuringiensis subspecies aizawai strain GC-91, Bacillus thuringiensis subspecies kurstaki, Bacillus thuringiensis subspecies kurstaki strain EG7841, Bacillus thuringiensis subspecies kurstaki strain SA-12, Bacillus thuringiensis subspecies kurstaki strain ABTS-351, Bacillus thuringiensis subspecies kurstaki strain SA-11, Bacillus thuringiensis subspecies tenebrionis strain SA-10, Chromobacterium subtsugae strain PRAA4-1, Isaria fumosorosea Apopka Strain 97, Burkholderia spp. strain A396, Bacillus thuringiensis subspecies aizawai strain ABTS-1857, Pseudomonas fluorescens strains AIP0069 or AIP1620, or Bacillus subtilis subsp. subtilis strain AIP61892. 37. The method of embodiment 35 or 36, wherein the biocontrol agent and the second biocontrol agent are applied simultaneously.

38. The method of embodiment 35 or 36, wherein the biocontrol agent and the second biocontrol agent are applied sequentially.

39. A method of controlling a plant pest comprising applying to a plant, a plant part, a seed, or an area of cultivation a biocontrol agent, wherein the biocontrol agent comprises:

(a) an effective amount of a bacterial strain deposited as NRRL No. B-67754, NRRL No. B- 67651, or NRRL No. B-67653; or

(b) an effective amount of a supernatant, fermentation product, fdtrate, or extract derived from a whole cell culture of a bacterial strain deposited as NRRL No. B-67754, NRRL No. B- 67651, or NRRL No. B-67653; wherein said effective amount controls the plant pest.

40. The method of embodiment 39, wherein said effective amount of the bacterial strain comprises at least about 10 ⁴ to 10 ¹⁶ colony forming units (CFU) per hectare or at least about 10 ⁶ to 10 ¹⁸ cells per hectare.

41. The method of embodiment 39 or 40, wherein the plant pest is an insect, nematode, fungus, fungal-like organism, virus, viroid, bacterium, or a protozoan pathogen.

42. The method of any one of embodiments 39-41, wherein said plant pest comprises one or more fungal pathogens and/or fungal-like pathogens selected from the group consisting of Aspergillus flavus, Aspergillus spp., Albugo occidentalis, Albugo spp., Alternaria solani, Alternaria spp., Apiognomonia errabunda, Apiognomonia veneta, Apiognomonia spp., Armillaria mellea, Armillaria spp., Bipolaris maydis, Botrytis cinerea, Botrytis squamosa, Botrytis spp., Botryosphaeria dothidea, Botryosphaeira spp., Blumeriella jaapii, Blumeriella spp., Bremia lactucae, Bremia spp., Cladosporium carpophilum, Cladosporium caryigenum, Cladosporium spp., Colletotrichum acutatum, Colletotrichum graminicola, Colletotrichum cereale, Colletotrichum gloeosporiodes, Colletotrichum sublineolum, Colletotrichum spp, Cochliobolus heterostrophus, Corynespora cassiicola, Corynespora spp., Discula fraxinea, Cercospora sojina, Cercospora beticola, Cercospora spp., Blumeria graminis f. sp. Tritici, Didymella bryoniae, Didymella spp., Elsinoe fawcetti, Elsinoe spp., Erysiphe necator, Erysiphe lager stroemiae, Erysiphe cichoracearum, Erysiphe spp., Eutypa lata, Eutypa spp., Fusarium graminearum, Fusarium solani, Fusarium oxysporum, Fusarium graminicola, Fusarium spp., Golovinomyces cichoracearum, Gibberella zeae, Gibberella spp., Gloeodes pomigena, Gymnosporangium juniperi-virginianae, Hemileia vastatrix, Leveillula taurica, Leveillula Mycosphaerella fijiensis, Mycosphaerella citri, Mycosphaerella pomi, Mycosphaerella spp., Macrophomina s p p .. Monosporasci is cannonballus, Monosporascus spp.. Monilinia fructicola, Monilinia laxa, Monilinia fructigena, Monilinia spp., Neofabraea spp., Podosphaera xanthii, Podosphaera leucotricha, Podosphaera spp., Phomopsis viticola, Phomopsis spp., Penicillium spp., Phakopsora meibomiae, Phakopsora pachyrizi, Phakopsora spp., Puccinia triticina, Puccinia recondita, Puccinia striiformis, Puccinia graminis, Puccinia spp., Pyrenophora trici-repentis, Rhizoctonia solani, Rhizoctonia spp., Rhizopus spp., Ramularia spp., Tilletia barclayena, Tilletia spp., Uncinula necator, Uncinula spp,. Uromyces betae, Uromyces spp., Phoma spp., Sclerotium rolfsii, Sclerotium spp., Sclerotinia minor, Sclerotinia sclerotiorium, Sclerotinia spp., Schizothyrium pomi, Schizothyrium spp., Septoria glycines, Septoria spp., Sphaerotheca pannosa, Sphaerotheca macularis, Sphaerotheca spp., Sphaceloma spp., Venturia inaequalis, Venturia spp., Verticillium spp., Wilsonomyces carpophilus, Wilsonomyces spp., Pythium cryptoirregulare, Pythium aphanidermatum, Pythium irregulare, Pythium sylvaticum, Pythium myriotylum, Pythium ultimum, Pythium spp., Phytophthora capsid, Phytophthora nicotianae, Phytophthora infestans, Phytophthora tropicalis, Phytophthora sojae, Phytophthora spp., Peronospora belbahrii, Peronospora lamii, Peronospora farinosa, Peronospora spp., Pseudoperonospora cubensis, Pseudoperonospora spp., Bremia spp., Plasmopara viticola, Plasmopara obduscens, Plasmopara spp., Basidiophora spp., Plasmodiophora brassicae, and P las modi ophora spp.

43. The method of embodiment 41, wherein said fungal and/or fungal-like pathogen is a Colletotrichum spp., Phytophthora spp.. Podosphaera spp., Aspergillus spp., Mycosphaerella. spp., Uncinula spp., or Botrytis spp.

44. The method of embodiment 43, wherein said fungal and/or fungal -like pathogen is Colletotrichum sublineolum, Phytophthora infestans, Podosphaera xanthii, Aspergillus flavus, Mycosphaerella fijiensis, Uncinula necator, or Botrytis cinera.

45. The method of any one of embodiments 39-41, wherein said plant pest comprises one or more bacterial pathogens selected from the group consisting of Acidovorax avenae, Burkholderia gladioli, Candidatus Liberibacter spp., Erwinia amylovora, Erwinia ananas, Erwinia chrysanthemi, Erwinia dissolvens, Erwinia herbicola, Erwinia rhapontic, Erwinia stewartiii, Erwinia tracheiphila, Erwinia uredovora; Pectobacterium carotovorum, Pseudomonas syringae, Streptomyces scabies, Xanthomonas campestris, Xanthomonas axonopodis, Xanthomonas fragariae; Xanthomonas translucens, and Xylella fastidiosa.

46. The method of any one of embodiments 39-45, wherein the biocontrol agent is applied to the plant or plant part after harvest. 47. The method of any one of embodiments 39-46, wherein the biocontrol agent is applied by dipping, drenching, flooding, fogging, spraying, dusting, or injecting.

48. The method of any one of embodiments 39-47, wherein said effective amount of the bacterial strain comprises at least about 10 ⁶ to 10 ¹² CFU/100 gallons of water or at least about 10 ⁸ to 10 ¹⁴ cells/ 100 gallons of water.

49. The method of any one of embodiments 39-48, wherein a second biocontrol agent is applied.

50. The method of embodiment 49, wherein the second biocontrol agent comprises Bacillus amyloliquefaciens strain D747, Bacillus subtilis strain QST 713, Bacillus amyloliquefaciens strain GB03, Bacillus subtilis strain FBI 7, Bacillus pumilus strain QST 2808, Bacillus pumilus strain GB34, Bacillus subtilis var. amyloliquefaciens strain FZB24, Bacillus finnus strain CNMC 1-1582, Streptomyces lydicus strain WYEC108, Streptomyces griseoviridis strain K61, Agrobacterium radiobacter strain 1026, Agrobacterium radiobacter strain K84, Pseudomonas fluorescens strain A506, Bacillus thuringiensis subspecies aizawai strain GC-91, Bacillus thuringiensis subspecies kurstaki, Bacillus thuringiensis subspecies kurstaki strain EG7841, Bacillus thuringiensis subspecies kurstaki strain SA-12, Bacillus thuringiensis subspecies kurstaki strain ABTS-351, Bacillus thuringiensis subspecies kurstaki strain SA-11, Bacillus thuringiensis subspecies tenebrionis strain SA-10, Chromobacterium subtsugae strain PRAA4-1, Isaria fumosorosea Apopka Strain 97, Burkholderia spp. strain A396, Bacillus thuringiensis subspecies aizawai strain ABTS-1857, Pseudomonas fluorescens strains AIP0069 or AIP1620, or Bacillus subtilis subsp. subtilis strain AIP61892.

51. The method of embodiment 49 or 50, wherein the biocontrol agent and the second biocontrol agent are applied simultaneously.

52. The method of embodiment 49 or 50, wherein the biocontrol agent and the second biocontrol agent are applied sequentially.

53. A method of treating or preventing a plant disease comprising applying to a plant, a plant part, a seed, or an area of cultivation a biocontrol agent, wherein the biocontrol agent comprises:

(a) an effective amount of a biocontrol agent comprising a bacterial strain deposited as NRRL No. B-67754, NRRL No. B-67651, or NRRL No. B-67653; or

(b) an effective amount of a supernatant, fermentation product, fdtrate, or extract derived from a whole cell culture of a bacterial strain deposited as NRRL No. B-67754, NRRL No. B-67651, or NRRL No. B-67653; wherein the effective amount treats or prevents the plant disease.

54. The method of embodiment 53, wherein said effective amount of the bacterial strain comprises at least about 10 ⁴ to 10 ¹⁶ CFU per hectare or at least about 10 ⁶ to 10 ¹⁸ cells per hectare..

55. The method of embodiment 53 or 54, wherein the plant disease is wherein the plant disease is Asian Soybean Rust (ASR), gray mold, leaf spot, Frogeye Leaf Spot, Early Blight, Damping off complex, Brown Patch, black scurf, root rot, belly rot, Sheath Blight, Powdery Mildew, Anthracnose, Black Sigatoka, Anthracnose leaf spot, Downy Mildew, Pythium Blight, Late Blight, Fusarium Head Blight, sudden death syndrome (SDS), Fusarium Wilt, Com Stalk Rot, Brown Rust, Black Rust, Yellow Rust, Wheat Rust, Rust, Apple Scab, Post-bloom Fruit Drop, Gummy Stem Blight, Greasy Spot, Com Stalk Rot, Cherry Blossom Blight, Damping Off, Fire Blight, Citms Greening Disease, Clubroot, Verticillium Wilt, or Brown Rot.

56. The method of any one of embodiments 53-55, wherein the biocontrol agent is applied to the plant or plant part after harvest.

57. The method of any one of embodiments 53-56, wherein the biocontrol agent is applied by dipping, drenching, flooding, fogging, spraying, dusting, or injecting.

58. The method of any one of embodiments 53-57, wherein said effective amount of the bacterial strain comprises at least about 10 ⁶ to 10 ¹² CFU/100 gallons of water or at least about 10 ⁸ to 10 ¹⁴ cells/ 100 gallons of water.

59. The method of any one of embodiments 53-58, wherein a second biocontrol agent is applied.

60. The method of embodiment 59, wherein the second biocontrol agent comprises Bacillus amyloliquefaciens strain D747, Bacillus subtilis strain QST 713, Bacillus amyloliquefaciens strain GB03, Bacillus subtilis strain FBI 7, Bacillus pumilus strain QST 2808, Bacillus pumilus strain GB34, Bacillus subtilis var. amyloliquefaciens strain FZB24, Bacillus finnus strain CNMC 1-1582, Streptomyces lydicus strain WYEC108, Streptomyces griseoviridis strain K61, Agrobacterium radiobacter strain 1026, Agrobacterium radiobacter strain K84, Pseudomonas fluorescens strain A506, Bacillus thuringiensis subspecies aizawai strain GC-91, Bacillus thuringiensis subspecies kurstaki, Bacillus thuringiensis subspecies kurstaki strain EG7841, Bacillus thuringiensis subspecies kurstaki strain SA-12, Bacillus thuringiensis subspecies kurstaki strain ABTS-351, Bacillus thuringiensis subspecies kurstaki strain SA-11, Bacillus thuringiensis subspecies tenebrionis strain SA-10, Chromobacterium subtsugae strain PRAA4-1, Isaria fumosorosea Apopka Strain 97, Burkholderia spp. strain A396, Bacillus thuringiensis subspecies aizawai strain ABTS-1857, Pseudomonas fluorescens strains AIP0069 or AIP1620, ox Bacillus subtilis subsp. subtilis strain AIP61892.

61. The method of embodiment 59 or 60, wherein the biocontrol agent and the second biocontrol agent are applied simultaneously.

62. The method of embodiment 59 or 60, wherein the biocontrol agent and the second biocontrol agent are applied sequentially.

The following examples are offered by way of illustration and not by way of limitation.

EXAMPLES

Example 1: Microbial strains and Methods of Culturing

Example 1.1: Isolation and Culturing

Bacterial strain AIP075655 is described in WO 2020/077042, incorporated by reference herein. Bacterial strain AIP059286 is described in WO 2020/092381, incorporated by reference herein. The bacterial strain AIP015329 was isolated from soil. The bacteria strain was successfully cultured both in liquid and solid media. The bacterial strain maybe cultured in media known in the art, for example CHA media which consists of, per L, NaCl (5 g), tryptone (10 g), nutrient broth (8 g), CaCl ₂ (0.14 mM), MgCl ₂ .6H20 (0.2 mM), and MnCl ₂ .4H20 (0.01 mM). AIP015329 was grown in 50 ml of liquid medium in a 250 ml baffled flask for 48 hours at 28°C. CFU/ml may be determined by plating the AIP015329 culture at a dilution of 10 ^-5 on LB Agar, growing for 24 h at 28°C, then counting the colonies present. CFU/ml may be calculated using the following formula: c = n/Sxd where c is the concentration, CFU/mL, n is number of colonies, d is dilution factor, S is volume transferred to plate.

Table 3: Growth of bacterial strain AIP015329 For detached-leaf assays performed in the following examples, bacterial strains stored in a freezer (- 80°C) were each streaked onto Luria Bertani (LB) agar (25 g LB broth and 15 g agar per liter of deionized water) in an 8.5 -cm diameter plastic plate. A single colony from the plate was picked and placed in 50ml of either LB liquid broth or a liquid broth consisting of NaCl (5g), tryptone (10 g), nutrient broth (8 g), CaCl ₂ (0.14 mM), MgCl ₂ 6 H ₂ O (0.2 mM), and MnCl ₂ 4H ₂ O (0.01 mM) per liter of deionized water in a 250-mi flask. Calcium chloride (CaCL). MgCL, and MnCL were filtered sterilized using 0.22 pm vacuum filter and were then added to NaCl, tryptone, nutrient broth (which were autoclaved together). Cultures were harvested after 48 h by pelleting cells and re-suspending to original volume in sterile H ₂ O. Colony forming units (CFU/mL) were determined using standard dilution plate count method, and plates were inspected for signs of contamination. Sterile distilled water was added to achieve a final concentration needed in detached-leaf or growth chamber strain evaluations.

Example 2: Evaluation of activity of bacterial strain AIP015329 against Colletotrichum sublineolum (sorghum anthracnose)

Sorghum cultivar 12-GS9016-KS585 was grown in the greenhouse for a steady supply of leaf tissue for the bacterial strain evaluation. Fully expanded sorghum leaves from 4-6 week old plants were excised and cut into equal pieces, 2,5cm wide. Colletotrichum sublineolum, (obtained from the Dr. Isakeit laboratory' at Texas A&M University) and grown on 20% Oatmeal agar for 14 days was used for inoculum. The actively growing culture was flooded with sterile distilled water to dislodge the spores. The concentration of the spore suspension was then adjusted to 1 x 10 ⁶ spores/mL. Tween 20 was then added to the suspension to 0.05%.

The bacterial strain was applied to the leaf pieces by spraying 120 pL of the bacterial culture suspended in magnesium chloride buffer (1 x 10 ⁸ CFU/ml) using a ribbed skirt fine mist fingertip sprayer (ID-S009, Container & Packaging Supply, Eagle, ID), fitted to a 15 ml conical centrifuge tube (Fisher Scientific, Cat No.l4-59-53A). The treated leaf pieces were then plated on 1% water agar amended with 6- Benzylaminopurine (BAP) and incubated at room temperature in the dark. 24 hours post treatment, the leaf pieces were inoculated with a 30 pL droplet of C. sublineolum spore suspension, applied on each side of the mid-rib. The plates were then incubated in a growth chamber (Percival Scientific, Inc) set to a 12-hour photoperiod, maintained at 25°C and 95% relative humidity. The experimental design was a randomized complete block design with 3 replications. Pyraclostrobin was used as a synthetic fungicide standard at 10 ppm.

Seven days post inoculation, the leaf pieces were assessed for anthracnose severity on a scale of 0-4 according to Prom et ah, 2016 (Plant Path J. 15(1): 11-16), with few modifications. 0-No symptoms or chlorotic flecks, 1 -hypersensitive reaction with no acervuli, 2-lesions with minute and few acervuli, 3- lesions with minute and few acervuli <25% of the leaf tissue and 4-lesions with acervuli covering >25% of the leaf surface. To identify isolates with robust, reproducible activity, we performed a “confirmation screen” on all active isolates from the primary screen. Isolates went into confirmation screens a few weeks after being identified in the primary screen, using a freshly grown bacterial culture. The confirmation assay protocol was the same as the primary. Data was analyzed using analysis of variance (ANOVA) in JMP® (version 14.0.0; SAS Institute Inc., Cary, NC). Significant differences (P < 0.05) were observed among treatments.

Table 4: Control of anthracnose by bacterial strain AIP015329 using sorghum leaf pieces

³ Means separation analyzed using SAS JMP version 14.0 command LSMeans Tukey's HSD.

Example 3: Evaluation of activity of bacterial strain AIP015329 against Podosphaera xanthii (powdery mildew on cucurbits)

Healthy squash leaves were excised and cut into uniform leaf disks 35 mm in diameter using a large cork borer (C.S. Osborne & Co, Harrison, NJ). An experimental unit consisted of a single leaf disk, each treated with a suspension of the bacterium. Controls were non-inoculated and inoculated leaf disks and the synthetic fungicide tebuconazole at 10 ppm as an additional positive control . Bacterial strain AIP015329 was prepared as described above. Each leaf disk was sprayed with 200 pL of the treatment (bacterial suspension or synthetic fungicide) on the adaxial surface 24 hours before inoculation with the fungal pathogen. After treatment, leaf disks were incubated in the dark for 24 hours at 23 °C.

Leaf disks were inoculated on the treated leaf surface by spraying a conidial suspension (1 x 10 ⁶ conidia/ml of sterile distilled water) of P. xanthii (an isolate obtained from Dr. McGrath laboratory, Cornell University ). Treatments were placed into sealed clear plastic boxes and incubated for six days at 25 °C with a relative humidity of 80% and a 12 h photoperiod. Each treatment was rated on a disease severity scale from 0 to 4, with 0 being no symptoms and 4 being greater than 50% of the leaf disk covered with colonies. The number of powdery mildew colonies were also recorded for each treatment. Each treatment was replicated two to three times for each experiment, and the confirmation assay protocol was the same as the primary. Data was analyzed in SAS JMP version 14.0. Results are shown in Table 5.

Table 5: Control of powdery mildew by bacterial strain AIP015329 using squash leaf discs

³ Means separation analyzed using SAS JMP version 14.0 command LSMeans Tukey's HSD.

Example 4: Evaluation of activity of bacterial strain AIP015329 against Mycosphaerella fijiensis (black sigatoka)

The susceptible Musa cultivar Grand Nain (supplied by Green Earth, Melbourne, Florida) was used in this experiment. Plants were maintained in the greenhouse for a constant supply of disease-free leaves. Mycosphaerella fijiensis culture (ITC0489) obtained from the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria was used for inoculation.

Bacterial isolate application and inoculation were performed as follows: smaller leaf pieces (4 cm long x 3 cm wide) were cut from an excised leaf. Two of these pieces were placed in plastic petri dishes with an adaxial side appressed on water agar amended with 5 mg/liter gibberellic acid (Twizeyimana et al. 2007). Leaf pieces were sprayed with 120 m,E of bacterial isolate (1 x 10 ⁸ CFU/ml of sterile distilled water) or a fungicide control (mancozeb 10 ppm) using a fingertip sprayer (Container & Packaging Supply, Eagle, ID) fitted to a 15 ml conical centrifuge tube (Fisher Scientific, Cat No.14-59-53A). Petri dishes with leaf pieces were incubated at room temperature in the dark. 24 hours later, leaf pieces in petri dishes were inoculated with a mycelial suspension of M. fijiensis (120 μl per leaf piece) using a fingertip sprayer fitted to a 50 ml conical centrifuge tube. The suspension contained mycelial fragments scraped from growing cultures that were cut into smaller mycelial tips in sterile distilled water in 50 ml conical tubes using a homogenizer (Omni International, Kennesaw, GA). The suspension was filtered through two layers of cheesecloth and then stirred. 0.05% Tween 20 and 0.02% Silwet L-77 (Loveland Industries Inc., Greeley, CO) were added. Using a hemocytometer, the suspension was adjusted with sterile distilled water to a concentration of 1 x 10 ⁶ mycelial fragments/ml. A day after inoculation, plates were incubated in a growth chamber (Percival Scientific, Inc) set at 14 hours photoperiod, maintained at 25 °C and 90% relative humidity.

Data recorded w ere the most progressed stage on inoculated leaves, 3 to 4 w eeks after inoculation (Stover 1986). Data were analyzed using analysis of variance (AN OVA) in PROC GLM of SAS (version 9.4; SAS Institute Inc., Cary, NC) and significant differences ( P < 0.05) were observed among treatments.

Table 6: Control of black sigatoka by bacterial strain AIP015329 using Grand Nain leaf pieces ^a Means were compared using Tukey's honestly significant difference mean separation test with a 0.05 significance level.

Example 5: Evaluation of activity of bacterial strain AIP015329 against Phakopsora pachyrhizi (Asian Soybean Rust)

The susceptible soybean cultivar Williams 82 was used in strain evaluation using the detached-leaf technique (Twizeyimana and Hartman, 2010). Briefly, soybean seeds were sown in 18-cell plastic inserts that were filled with soil-less mix (Sunshine Mix, LC1 ; Sun Gro Horticulture Inc., Bellevue, WA) and placed inside a flat. Cells were fertilized at planting with slow-release pellets (Osmocote 19-6-12; 2 pellets per cm ² ). Flats were maintained inside a growth chamber (Percival Scientific, Inc., Boone, IA) maintained at 70% relative humidity (RH) with a daily cycle of 14 h of light and 10 h of darkness at 24° and 20°C, respectively.

Healthy soybean leaves were excised and cut into uniform leaf disks 35 mm in diameter using a large cork borer (C.S. Osborne & Co, Harrison, NJ). Leaf disks (each as an experimental unit) were sprayed on abaxial side with 120 μl of bacterial strain (1 x 10 ⁸ CFU/mL of sterile distilled water) and a fungicide control (azoxystrobin 0.5 ppm, Quadris, Syngenta, Greensboro, NC) using a fingertip sprayer (Container & Packaging Supply, Eagle, ID) fitted to a 15 mL conical centrifuge tube (Fisher Scientific, Cat No.14-59- 53A). Leaf disks were placed adaxial side down on saturated 20 x 20 cm filter paper (Whatman International Ltd., Kent, England) in a plastic container (Blister Box 20 x 20 cm, Placon, Madison, WI); two filter papers were used per box. Boxes with leaf disks were incubated at room temperature in the dark, 24 hours later, leaf disks in the box were inoculated with a spore suspension of P. pachyrhizi urediniospores (120 μl per leaf disk at 5 x 10 ⁴ urediniospores/mL of sterile distilled water) using an atomizer attached to an air compressor (Twizeyimana and Hartman, 2010). After inoculation, boxes were incubated in the dark for a period of 18 h followed by a cycle of 13 hours of light (40-60 mhioΐ m V ¹ ) at 21.5°C and 11 h of darkness at 22°C inside a growth chamber (Percival Scientific, Inc.) maintained at 80% RH. Prior to placing in a growth chamber, boxes were placed inside zip bags (Webster Industries, Peabody, MA).

Data recorded were numbers of uredinia in 1-cm diameter circle recorded 14 days after inoculation. Rust severity score for each replication was an average of the number of sporulating uredinia from two arbitrarily selected 1-cm diameter circles of leaf tissue from inoculated leaf disk. The number of sporulating uredinia per each replication was used to calculate percent disease control values as follows: 100 - [(number of sporulating uredinia from each treatment/number of sporulating uredinia from the inoculated-non treated treatment) x 100]. The percent disease control value for each treatment was the a verage of percent disease control values from both replications. Data were analyzed using analysis of variance (ANOVA) in PROC GLM of SAS (version 9.4; SAS Institute Inc., Cary, NC) and significant differences (P < 0.05) were observed among treatments.

Table 7: Control of Asian soybean rust by bacterial strain AIP015329 using soybean leaf disks ^a Means were compared using Tukey's honestly significant difference mean separation test with a 0.05 significance level.

Example 6: Preparation of Wettable Powder Formulations of AIP15329

A wettable powder formulation comprising bacterial strain AIP15329 was prepared using two different methods.

For the tray drying method, 829 grams of cell paste obtained from a liquid culture of AIP15329 was mixed with 18.24 g of glycerol and 72.95 g of microcel E using a food processor. Half of the cell paste was dried at 40°C to a water activity of less than 0.30, at which time it contained 3.53E11 CFU/g. The other half was dried at 25 °C and 50% RH to a water activity of less than 0.6, at which time it contained 4.75E11 CFU/g. The dried powder formulation was stored in heat sealed mylar pouches at 4°C.

For the spray drying method, 200 grams of cell paste obtained from a liquid culture of AIP15329 was mixed with 22 g of skim milk powder using an emulsion blender. This material was spray dried at outlet temperature of 70°C, at which time it contained 7.86E10 CFU/g. The dried powder fonnulation was stored in heat sealed mylar pouches at 4°C.

Example 7: Evaluation of activity of formulations of bacterial strain AIP015329 against Podosphaera xanthii (powdery mildew on cucurbits)

This evaluation followed the protocol used in Example 3. Briefly, leaf disks were each sprayed with 150 μL of different formulations of the bacterial strain AIP015329, tebuconazole 10 ppm (fungicide control) on the adaxial surface 24 hours before inoculation with the fungal pathogen. After treatment, leaf disks were incubated in the dark for 24 hours at 23 °C.

Leaf disks were inoculated on the treated leaf surface by spraying a conidial suspension (1 x 10 ⁶ conidia/ml of sterile distilled water) of P. xanthii. Inoculated and non-inoculated/not treated controls were added. Treatments were placed into sealed clear plastic boxes and incubated for six days at 25 °C with a relative humidity' of 80% and a 12 h photoperiod. Each treatment was rated on a disease severity scale from 0 to 4, with 0 being no symptoms and 4 being greater than 50% of the leaf disk covered with colonies. The number of powdery mildew colonies were also recorded for each treatment. This experiment was run once, with each treatment replicated three times. Data was analyzed in SAS JMP version 14.0. Results are shown in Table 8. Table 8: Control of powdery mildew by bacterial strain AIP015329 formulations using squash leaf discs ^a Means separation analyzed using SAS JMP version 14.0 command LSMeans Tukey’s HSD. Example 8: Evaluation of activity of formulations of bacterial strain AIP015329 against Phakopsora pachyrhizi (Asian Soybean Rust) This evaluation followed the protocol used in Example 5. Briefly, leaf disks were each sprayed with 150 µL of different formulations of the bacterial strain AIP015329, azoxystrobin (0.25 ppm) (fungicide control) on the abaxial surface with 150 μl using a fingertip sprayer (Container & Packaging Supply, Eagle, ID) fitted to a 15 mL conical centrifuge tube. Leaf disks were placed adaxial side down on saturated 20 × 20 cm filter paper in a plastic container (Blister Box 20 × 20 cm, Placon, Madison, WI); two filter papers were used per box. Boxes with leaf disks were incubated at room temperature in the dark, 24 hours later, leaf disks in the box were inoculated as described earlier. Inoculated and non-inoculated/not treated controls were added. After inoculation, boxes were maintained and incubated as described in the first-round evaluations. Data recorded were numbers of uredinia in 1-cm diameter circle recorded 14 days after inoculation. The number of sporulating uredinia per each replication was used to calculate percent disease control values as follows: 100 − [(number of sporulating uredinia from each treatment/number of sporulating uredinia from the inoculated-nontreated treatment) × 100]. Data were analyzed using analysis of variance (ANOVA) in PROC GLM of SAS (version 9.4; SAS Institute Inc., Cary, NC) and significant differences (P < 0.05) were observed among treatments (Table 9). Table 9: Control of Asian soybean rust by bacterial strain AIP015329 formulations using soybean leaf discs ^a Means were compared using Tukey's honestly significant difference mean separation test with a 0.05 significance level.

Example 9: Colorado Potato Beetle Leaf Disc Assay

A starter culture is prepared by filling a 96-well block with 1-ml (per well) LB media. Each well of the block is inoculated with a bacterial strain. This starter culture is grown at 30°C shaking at 225 rpms for 24 h. Assay cultures are prepared by filling two 48-well blocks with -1.7 ml (per well) media. Twenty-five μl from each well of the starter culture is added to the assay culture blocks. Assay block are grown at 30 °C for either 24, 48 or 72 hrs at 225 rpms.

After growth, the assay blocks are removed from the incubator/shaker and centrifuged for 20 min at 4000 rpms in order to pellet the microbial content. The supernatant is then poured off so that only the pellet remained. Pellets are then re-suspended in 1.7 ml buffer and placed on ice until they are used in the assay. All microbial preparations are applied within 12 h of preparation.

A single prefilter is placed in each well of a 24-well plate. 50 μl ddH20 is applied to each filter, to maintain the relative humidity throughout the experiment. Undamaged and uncurled potato leaves from a potato plant are selected for use. A #8 cork borer is used to make leaf discs, the center vein of the leaf is avoided. A single leaf disc is placed so the top-side of the leaf was facing up into each well of a 24-well plate, the leaf is placed so that it is not in contact with any of the sides of the plate. Microbial strains are provided as a liquid culture by microbiology in 48-well blocks with each well containing approx. 2250 μl suspension. 100 μl of 1% stock solution of surfactant (Silwet ECO spreader) is added to each well. The culture is thoroughly mixed and 40 μl is pipetted onto a potato leaf disc. This process is repeated so that every bacterial treatment is applied to two leaf discs. The treatment is allowed to spread over the entire leaf.

After treatments dry, 5-6, 2nd-instar CPB larvae are added to each well. CPB eggs are purchased from French Agricultural Research, Inc., Lamberton, MN. After adding 5-6 larvae to each well, the plates are sealed with a pressure-sensitive adhesive cover and 4 small holes are added above each well. Plates are then placed in a Percival incubator and maintained at 26 °C and 55% RH with 12/12 light: dark photoperiod for 24 h. After 24 h, plates are evaluated for the percent of each leaf disc that is consumed by the CPB larvae. Plates are than returned to the incubator. Forty-eight hours post-treatment, the plates are removed from the incubator and CPB mortality is recorded for any wells in which <20% estimated leaf consumption occurred at the 24 h read. A microbe is considered active on CPB when less than 20% of the leaf disc has been consumed and/or there is greater than 80% mortality in three or more independent repetitions. Example 10: Lepidopteran Diet Overlay Assay

Fall armyworm (FAW), com earworm (CEW), European com borer (ECB) southwestern com borer (SWCB) and diamond backed moth (DBM) eggs are purchased from a commercial insectary (Benzon Research Inc., Carlisle, PA). The FAW, CEW, ECB and BCW eggs are incubated to the point that eclosion occurred within 12 hrs of the assay setup. SWCB and DBM are introduced to the assay as neonate larvae. Assays are carried out in 24-well trays containing multispecies lepidopteran diet (SOUTHLAND PRODUCTS INCORPORATED, Lake Village, AR). Whole culture microbial suspensions are applied to the surface of the diet (diet overlay) and allowed to evaporate and soak into the diet. The bioassay plates are sealed with a plate sealing film vented with pin holes. The plates are incubated at 26 °C at 65%RH on a 16:8 daymight cycle in a Percival for 5 days. The assays are assessed for level of mortality, growth inhibition and feeding inhibition. A microbe is considered active on CEW or FAW when mortality, growth inhibition and/or feeding inhibition is greater than the controls in three or more independent repetitions.

Example 11: Western Com Rootworm Diet Overlay Assay:

Western com rootworm (WCR) eggs are purchased from French Agricultural Research, Inc., Lamberton, MN. 60 μl volume of whole culture microbial suspension is inoculated on the top surface of diet in well/s of 24-well plate (Cellstar, 24-well, Greiner Bio One) and allowed to dry. Each well contains 500 μl diet (Marrone et al, 1985). Fifteen to twenty neonate larvae are introduced in each well using a fine tip paint bmsh and the plate is covered with membrane (Viewseal, Greiner Bio One). The bioassay is stored at ambient temperature and scored for mortality, growth inhibiton, and/or feeding inhibition at day 4. A microbe is considered active on WCR when it has greater than 70% mortality in three or more independent repetitions.

Example 12: Stink Bug feeding assay

Southern Green stink bugs (SGSB) are reared at the AgBiome facility in RTP, NC. Only healthy second instar SGSB are used in the assay. One ml of a 50:50 whole culture microbe: sucrose suspension is added to 4 wells of a 24 well plate, which is then covered with stretched parafilm that served as a feeding membrane to expose the SGSB to the diet/sample mixture. The plates are incubated at 25°C:21°C, 16:8 daymight cycle at 65%RH for 5 days. Mortality is assessed for each sample. A microbe is considered active for SGSB when mortality is greater than 60% in three or more independent repetitions.

Example 13: Aphid contact assay

Aphid contact assays are performed to test the efficacy of AIP015329 on aphids.

Example 13.1: Aphid primary contact assay

The following in vitro assay is used in the primary screen to determine if the bacterial culture or sample has contact activity against aphids (soybean aphids, Aphis glycines (SBA) and green peach aphids, Myzus persicae (GPA)). This assay also is used to guide to determine the active portion or fraction of a bacterial culture, such as a whole cell broth, or of a supernatant derived from a whole cell broth, and to assist in determing its active components. Aphid colonies are initiated at AgBiome from insects from North Carolina State University and Michigan State University. Five to ten aphids are removed from infested leaves with a paintbrush and placed on top of leaf discs cut from fresh un-infested leaves of 3-4 week old radish (or soybean) plants using a cork borer. One leaf disc is placed into each well of a 24-well plate on top of the agar solution. Plates are sealed with a pressure-sensitive adhesive fdm and 4 small air holes are punched into the fdm over each well. Plates are placed in a Percival growth chamber for 24 hours in order to allow aphids to acclimate before microbial application.

Leaf discs are removed from wells and submerged into treatment 3 times and then returned to each well. 24-well plates are placed under a fan to dry. Plates are re-sealed with a fdm held in Percival and observations for mortality, reproduction, and honeydew production are taken 3 days post-treatment.

Bacterial cultures are considered active when high aphid mortality, high nymph mortality, low reproduction and low honey dew production are observed in three or more independent assays.

Example 13.2: Insecticidal activity of supernatant and cell pellet of bacterial strain AIP015329

The following in vitro assay is performed to determine contact insecticidal activity of a bacterial strain against the aphid. Bacterial strains grown in liquid media are removed from the incubator/shaker and centrifuged for 20 min at 4000 rpms in order to pellet the cells and cellular debris. The supernatant (cell-free broth and/or spent media) is then separated from the pellet and diluted 0.5x. The pellets are ished in buffer and then re -suspended in the growth volume.

The samples are rated on a scale from high activity (+++) to no activity (-) observed. High active (+++) rating is made when high adult mortality, high nymph mortality, low reproduction and low honey dew production are observed in three or more independent assays.

Example 14: Wettable powder formulations

The cultures produced in Example 1 are centrifuged for 20 minutes at 10,000 rpm to produce a pellet. The supernatant is poured off and another volume of liquid culture is added to the previous pellet and centrifuged again.

A wettable powder formulation is produced by adding 10% (by mass of pellet) of glycerol to the cell pellet and then mixing with a spatula. 40% (by mass) of Microcel E is transferred to a food processor and the glycerol/pellet is poured over the Microcel. This material is blended using the knife blade attachment of the food processor for not more than 10 seconds, lire product is dried overnight at 40°C to a water activity of approximately 0.3. The dried powder formulation is stored in vacuum sealed mylar pouches at 22°C.

Example 15: Field Evaluations Grape field trials were performed to test the efficacy of AIP075655, AIP059286, and AIP015329 on grape powdery mildew caused by the fungus Uncinula necator and on Botrytis gray mold of grapes caused by Botrytis cinerea.

Example 15.1: Evaluation of activity of bacterial strains against Uncinula necator (grape powdery mildew) Two grape field trials were performed during 2020 to test the efficacy of AIP075655, AIP059286, and AIP015329 on grape powdery mildew caused by the fungus Uncinula necator. One trial was located in Kerman, California and the other trial was located in Parkdale, Oregon. Each of the strains were tested at rates of 2.5 and 5 pounds of product per acre and were compared to the chemical fungicide standard Rally® (myclobutanil) at standard field rates. Treatment applications began at flowering and applications were made on a 7 day spray interval. At the Parkdale, OR site a total of 6 applications were made, while at the Kerman, CA site a total of 4 applications were made. All treatments contained a commercial adjuvant to improve the spreading of the application. Visual ratings of percent incidence of powdery mildew on grape clusters were collected on a per plot basis. Data were analyzed using R and ARM software. Means were compared with a Least Significant Difference test at the p=0.05 level. Statistically significant differences between means are indicated by different letters following the mean value of each treatment in Table 10.

Table 10: Control of Grape Powdery Mildew by bacterial strains in two field trials

All tested rates of the three strains demonstrated statistically significant reduction in the incidence of powdery mildew over the untreated plots. All tested rates of the three strains were statistically similar to the Rally® standard, with the exception of the 2.5 lb rate of AIP075655. Considering the three strains tested performed as well as the standard, each of these strains demonstrates fungicidal efficacy against grape powdery mildew.

Example 15.2: Evaluation of activity of bacterial strains against Botrytis cinerea (Botrytis gray mold of grapes)

A grape field trial was performed in Dundee, New York to test the efficacy of AIP075655, AIP059286, and AIP015329 on Botrytis gray mold of grapes caused by Botrytis cinerea. Each of the strains were tested at rates of 2.5 and 5 pounds of product per acre and were compared to the chemical standard Elevate® (fenhexamid) at standard field rates. A total of four treatment applications were made beginning at flowering, followed by bunch closure, verasion and two weeks after verasion. Visual ratings of percent severity of Botrytis gray mold on grape clusters were collected on a per plot basis. Data were analyzed using R and ARM software. Means were compared with a Least Significant Difference test at the p=0.05 level. Statistically significant differences between means are indicated by different letters following the mean value of each treatment in Table 11.

Table 11. Control of Grape Botrytis Gray Mold by bacterial strains in a field trial

All tested rates of the three strains demonstrated statistically significant reduction in the incidence of powdery mildew over the untreated plots. All tested rates of the three strains were statistically similar to the Elevate® standard. Considering the three strains tested performed as well as the standard, each of these strains demonstrates fungicidal efficacy against Botrytis gray mold of grapes.

Example 16, Rhizoctonia Damping-Off Assay-- Soybean Mock Seed Treatment/In-Furrow

11-14 day old Rhizoctonia solani infested grain is ground. The ground inoculum is screened through a #10 screen to remove any grain that is not ground well. The ground, screened infected grains are added to Fafard Superfine Germination media at 1.5 grams of ground inoculum to 1 liter of soil mix by volume. Germination mix, inoculum, and 1 liter of water per 75 liters of germination media are added to a cement mixer and mix until everything is well incorporated. The well incorporated media-inoculum material is placed into a secondary holding container with a lid and held at 20°C for 18 hours before using in the assay.

606-cell planting trays are filled with inoculated germination media making sure to not pack the media too firmly. One soybean seed is sown per 606 cell, planting at a depth of 1.5 to 2cm leaving the planting holes open if applying treatments as a liquid formulation. Individual planting cells are treated with one of the re-suspended strains set forth in Table 2 at 3ml per cell/seed. The seed treatment is directly over the top of the seed. Once treatments is applied, the shake flats is shaken lightly shake to close planting holes. The planting trays are lightly watered and placed in a humidity dome on the flat. After 3-4 days, flats are checked for moisture and lightly watered as needed to ensure cells are evenly moist. The humidity dome is replaced after watering.

Data Collection and Results: After 10-12 days, the assay is evaluated to determine the number of seeds that germinated. Data is reported as the % of seeds that germinated out of a total of 6 seeds per treatment.

Example 17, Field Trials Against Various Plant Pathogens for the Various Bacterial Strains or active variants thereof

The various bacterial strains recited in Table 2 are applied to the crops listed in Table 12 in the field under the current agronomic practices at listed in Table 12 to achieve uniform plant coverage and follow proper agronomic practices. Treatments are applied preventative ly and/or curative ly at the appropriate timings per disease.

Table 12: Field trials against plant pathogens

The specific treatments are outlined below:

Treatment List:

1. Non-Inoculated, untreated Check 2. Inoculated Check

3. Chemical control chosen by cooperator applied at label instructions

4. (Optional) Biological control Serenade applied at label instructions

5. Experimental bacterial treatment(s) at 5 lb/Acre and/or 2.5 lb/Acre Example 18. Field trials against various plant pathogens for the bacterial strains or active variants thereof employing seed treatments

A seed treatment formulation is made by mixing 10 g of a formulated bacterial strain recited herein or an active variant thereof plus 30ml water plus 15ml Unicoat Polymer. The weighed out seed is placed in a sterilized mason jar. An appropriate amount of seed treatment solution based on the seed weight (,05ml/25g seed) is added and the mixture is shaken for 60 seconds or until the seeds were visually well coated. The seeds are placed into a single layer in a foil roasting pan and placed under a laminar flow hood for 1 hour or until seeds are dry. Once the seeds dry, they are placed in an air tight container and stored at RT.

The seed treatment formulation is applied to seed of the crops listed in Table 13 prior to being planted into the field. Bacterial strain treatments are applied for preventative control of the diseases and at the application rates in Table 14. The specific treatments are outlined below.

Table 13: Crops for seed treatments Seed Treatment Trial Treatment List:

1. Non-inoculated, untreated Check

2. Inoculated Check

3. Disease appropriate Seed Treatment Chemical Check chosen and applied by cooperator

5. Experimental bacterial Seed Treatment(s)

Table 14: Trials for seed treatments Example 19. Field trials against various fungal pathogens for the bacterial strains or active variants thereof employing in-furrow treatments

The various bacterial strains or active variants thereof recited in Table 1 are applied to the crops listed in Table 13 as in-furrow treatments at time of planting as preventative control for the diseases and at the treatment rates listed in Table 15. The specific treatments are outlined below: In-Furrow Trial Treatment List:

1. Non-inoculated Check

2. Inoculated Check

3. In-Furrow bacterial treatment(s) 5g/L + Capsil at 6oz/100 Gallons at 15 Gallons/Acre

4. Disease appropriate in-furrow chemical check as chosen and applied by cooperator.

Table 15: Trials for in-furrow treatments

All publications and patent applications mentioned in the specification are indicative of the level of skill of those skilled in the art to which this invention pertains. All publications and patent applications are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.

Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it will be obvious that certain changes and modifications may be practiced within the scope of the appended claims.