COMPOSITIONS AND METHODS FOR OPTIMIZING CLEAVAGE OF RNA BY RNASE H

Title:

COMPOSITIONS AND METHODS FOR OPTIMIZING CLEAVAGE OF RNA BY RNASE H

Document Type and Number:

WIPO Patent Application WO2005089268

Kind Code:

A3

Abstract:

The present invention provides compositions and methods for the optimization of cleavage of RNA species by RNase H. In some emboediments, the invention provides oligonucleotides that possess two or more regions of differing conformation, and at least one transitional nucleobase positioned between the regions that is capable of modulating transfer of the helical conformation characteristic of the region bound to the 3'hydroxy thereof, to the region bound to the 5' hydroxyl thereof.

Inventors:

MIGAWA MICHAEL T (US)
LIMA WALTER F (US)
SWAYZE ERIC E (US)
NICHOLS JOSHUA (US)
WU HONGJIANG (US)
PRAKASH THAZHA P (US)
WYRZYKIEWICZ TADEUSZ KRZYSZTOF (US)
BHAT BALKRISHEN (US)
CROOKE STANLEY T (US)

Application Number:

PCT/US2005/008428

Publication Date:

May 18, 2006

Filing Date:

March 15, 2005

Export Citation:

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Assignee:

ISIS PHARMACEUTICALS INC (US)
MIGAWA MICHAEL T (US)
LIMA WALTER F (US)
SWAYZE ERIC E (US)
NICHOLS JOSHUA (US)
WU HONGJIANG (US)
PRAKASH THAZHA P (US)
WYRZYKIEWICZ TADEUSZ KRZYSZTOF (US)
BHAT BALKRISHEN (US)
CROOKE STANLEY T (US)

International Classes:

C12Q1/68; A61K48/00; C07H21/04; C12N15/11; C12N15/85

Foreign References:

US20030096770A1	2003-05-22
US20020160379A1	2002-10-31
US20030171211A1	2003-09-11

Other References: