Title:
COMPOUND ENZYME AND PREPARATION METHOD FOR RESISTANT DEXTRIN
Document Type and Number:
WIPO Patent Application WO/2022/156211
Kind Code:
A1
Abstract:
Provided are a compound enzyme and a method for preparing resistant dextrin by cooperating with synchronous extrusion. The method comprises: selecting the proportion of compound amylase according to different glycosidic bond action sites; performing high shear co-extrusion processing on a compound enzyme and starch; and by means of thermal-mechanical multi-physical-field coupling (µm-level non-directional shearing) and synergistic enzymolysis fixed-point depolymerization (nm-level specific shearing) in an extrusion cavity, finely adjusting and controlling the breaking order and degree of a linear chain and a branch chain of the starch so as to form differential chain length distributions. According to the method, a built-in dual-screw cavity is used as a reactor for efficient enzymolysis of the starch, and under the condition of increasing the axial diffusion rate, the residence time, and the action area of the reactor, the dosage of an enzyme liquid is reduced, thereby forming the high-crystallinity resistant dextrin (Mw: 0.5-8 kDa).
Inventors:
XU ENBO (CN)
LIU DONGHONG (CN)
TANG JUNYU (CN)
ZHOU JIANWEI (CN)
CHEN JIANLE (CN)
TIAN JINHU (CN)
CHENG HUAN (CN)
YE XINGQIAN (CN)
LIU DONGHONG (CN)
TANG JUNYU (CN)
ZHOU JIANWEI (CN)
CHEN JIANLE (CN)
TIAN JINHU (CN)
CHENG HUAN (CN)
YE XINGQIAN (CN)
Application Number:
PCT/CN2021/114247
Publication Date:
July 28, 2022
Filing Date:
August 24, 2021
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
International Classes:
C12P19/04; C12N9/26; C12N9/44; C12P19/14; C12P19/16; C12P19/22
Foreign References:
| CN112725312A | 2021-04-30 | |||
| CN111154818A | 2020-05-15 | |||
| CN104561191A | 2015-04-29 | |||
| JP2019001735A | 2019-01-10 |
Other References:
KRZYŻANIAK WOJCIECH, BIAŁAS WOJCIECH, OLESIENKIEWICZ ARTUR, JANKOWSKI TOMASZ, GRAJEK WŁODZIMIERZ: "CHARACTERISTICS OF OLIGOSACCHARIDES PRODUCED BY ENZYMATIC HYDROLYSIS OF POTATO STARCH USING MIXTURE OF PULLULANASES AND ALPHA-AMYLASES", ELECTRONIC JOURNAL OF POLISH AGRICULTURAL UNIVERSITIES, WYDAWNICTWO AKADEMII ROLNICZEJ WE WROCLAWIU, WROCLAW, PO, vol. 6, no. 2, 31 December 2003 (2003-12-31), PO , XP055953109, ISSN: 1505-0297
SHEN XINMIAO, WANG RUI;WANG YAN;MA YUJIA;WANG CHUNHUI;TANG YANJUN: "Study on Preparation of Potato Fat Simulation by Complex Enzymes", AGRICULTURAL SCIENCE & TECHNOLOGY AND EQUIPMENT, vol. 1, 31 January 2020 (2020-01-31), XP055953026, ISSN: 1674-1161, DOI: 10.16313/j.cnki.nykjyzb.2020.01.021
SHEN XINMIAO, WANG RUI;WANG YAN;MA YUJIA;WANG CHUNHUI;TANG YANJUN: "Study on Preparation of Potato Fat Simulation by Complex Enzymes", AGRICULTURAL SCIENCE & TECHNOLOGY AND EQUIPMENT, vol. 1, 31 January 2020 (2020-01-31), XP055953026, ISSN: 1674-1161, DOI: 10.16313/j.cnki.nykjyzb.2020.01.021
Attorney, Agent or Firm:
HANGZHOU HUACHEN JOINT INTELLECTUAL PROPERTY AGENCY (SPECIAL GENERAL PARTNERSHIP) (CN)
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