Background of the Invention Most newly manufactured cellulose-containing fabrics have a handle that is rather hard and stiff unless they are treated with finishing components. In addition, the fabric surface appears not smooth due to small fuzzy fibers protruding from its surface.

Furthermore, after a relatively short period of wear, pilling appears on the fabric surface, giving it an unappealing, worn look. For these reasons, improving fabric handle, appearance and pilling resistance is one of the main goals of the textile industry. However, only partial success has been achieved.

A high degree of fabric softness and smoothness can be obtained by using fine, i. e., low-denier, yarns in weaving. However, the resulting cost is high as the loom output decreases proportionately with the weft yarn diameter.

A less expensive way of ensuring a soft and smooth fabric handle is to impregnate the finished fabric with a softening agent, typically a cationic, sometimes silicone-based, surface active compound. However, this treatment does not remove pills and fuzz. Furthermore, the fabric obtains a somewhat greasy handle and is not wash-proof and its moisture absorbency is often considerably reduced.

One chemical method is crosslinking fibers to reduce the fibrillation (Nicolai et al, 1996, Textile Res. J. 66 (9) 575-580). However, this method causes a decrease in fiber tenacity.

Another known method for obtaining a soft and smooth fabric is treating cellulosic fabrics with cellulases. See, Bazin et al., "Enzymatic Bio-Polishing of Cellulosic Fabric,"presented at the 58th Congres of the Association of Chemists and the Textile Industry in Mulhouse, France (October 25,1991) and Asferg et al., "Softening and polishing of cotton fabrics by cellulase treatment,"ITB DyeingIPrintingIFinishing (February 1990). Cellulase treatment of the fabric surface improves fabric quality with respect to handle, appearance and pilling resistance. The most important effects are less fuzz and pilling, increased gloss/luster, improved fabric handle, increased durable softness, and improved water absorbency. These effects are referred to as biopolishing effects. The particular conditions that are utilized are important in determining the outcome of the treatment.

Many processes require exposing the fabric to mechanical agitation to obtain satisfactory biopolishing results. See, for example, WO 9320278; Cavaco-Paulo et al.

(1994, Biocatalysis :10 353-360); and Cavaco-Paulo et al. (1996, Textile Res. J.

66: 287-294). However, under some conditions, significant weight loss and strength loss are also observe.

Current methods in cellulase biopolishing are mainly batch processes. The common continuous or semi-continuous processes such as pad-steamer/J-box are not used because they do not provide high mechanical action and use only small volumes of solution and thus result in insufficient and/or uneven biopolishing. For example, non- uniform biopolishing can result from the use of a cellulase complex, in part because different cellulases exhibit different affinities for cellulose and thus are differentially bound by the fabric.

Thus, there is a need in the art for effective biopolishing methods that can be used in conventional continuous or semi-continuous processes.

Summarv of the Invention The present invention provides a method for treating a cellulose-containing fabric to improve at least one polished property of the fabric. The method is carried out by the steps of (a) contacting the fabric with an aqueous bulk solution comprising a cellulase, wherein the cellulase has a low affinity for cellulose, and (b) subjecting the contacte fabric to high temperature.

Preferably, the method is carried out in a continuous or semi-continuous apparats. In these embodiments, the method further comprises, after step (a), removing the contacte fabric from the bulk solution. In preferred embodiments, the fabric is contacte with the bulk solution for less than about 5 minutes, most preferably, for less than about 1 minute. The contacting and subjecting steps may be performed sequentially or simultaneously.

The polished property may be one or more of pilling note, handle, and appearance. In preferred embodiments, the methods of the invention result in an improvement in pilling note of at least about 0.25; more preferably, at least about 0.5; and most preferably, at least about 1.0.

The low-affinity cellulases are preferably enzymes that exhibait thermostable cellulase activity. Typically, the bulk solution contains less than about 200 CMCU/ml of cellulase activity, preferably less than about 100 CMCU/ml, and more preferably, less than about 50 CMCU/ml.

In other aspects, the invention provides methods for combine biopolishing and dyeing, or combine biopolishing and scouring. In these embodiments, the aqueous solution with which the fabric is contacte contains, in addition to the low-affinity cellulase, other appropriate components such as, e. g., dyes and auxiliary compound.

Detailed Description of the Invention The present invention provides biopolishing methods that enhance the quality of cellulosic fabrics. The methods are carried out by (i) contacting a cellulosic fabric, preferably in a continuous or semi-continuous apparats, with an aqueous bulk solution comprising at least one cellulase that exhibits a low affinity for cellulose and (ii) subjecting the cellulase-contacted fabric to a high temperature.

Biopolishing as used herein refers to a treatment that is directe towards improving one or more of the following properties: fabric handle, appearance, and pilling resistance. The methods allow uniform action by the cellulase (s) on the fabric and result in mesurable improvements in one or more of these properties, while minimizing loss of fabric weight and/or fabric strength and obviating the need for mechanical agitation. The present invention minimizes loss of cellulase from the aqueous solution via adsorption to the fabric, and thereby allows the use of conventional semi-continuous or continuous textile industry equipment. The methods of the invention can also be combine with processes such as alkaline chemical preparation, fabric dyeing, printing, and finishing, thereby affording increased flexibility in textile manufacturing. Furthermore, the simultaneous use of other enzymes, such as, e. g., lipase, protase, hemicellulases, and/or pectinases, allows the simultaneous removal of cellulosic and non-cellulosic materials. Finally, the methods of the invention can reduce the formation of lint dust during subsequent sewing and home laundry of fabrics treated according to the invention.

Cellulosic fabrics as used herein encompass both knitted and woven structures made from cellulosic fibers, including, without limitation, cotton, flax, ramie, hemp, jute, rayon/viscose, tencel/lyocell, or their blends, as well as fabrics made blends of cellulosic fibers and other natural and/or manade fibers such as, e. g., wool, silk, polyester, nylon, and the like.

A continuous or semi-continuous apparats as used herein refers, without limitation, to conventional equipment such as, e. g., pad-steamer-washing boxes or pad- J boxes, in which the fabric is wetted by contact with a bulk solution, and, once passed through, is no longer in direct contact with the bulk solution. This is distinguished

from equipment in which the fabric is in continuous contact with a bulk solution throughout the treatment (batch methods). In a batch apparats, the liquid: fabric ratio (weight of solution used per weight of fabric) is generally greater than about 400%, as compare with a wet pick-up (weight of solution absorbe per weight of fabric) of between about 50% and about 150% in a continuous or semi-continuous apparats. It will be understood that the present invention encompasses the use of any configuration or apparats in which the fabric is only exposed to the bulk solution for a short time relative to the total treatment time, with or without padding to remove excess solution from the fabric.

"High temperature"as used herein refers to temperatures above about 65°C, preferably above about 70°C, and most preferably above about 90°C.

Cellulases In practicing the present invention, a cellulosic fabric is contacte with a cellulase that exhibits a low affinity for cellulose. As used herein, a cellulase or cellulolytic enzyme is an enzyme that hydrolyzes cellulose, including, without limitation, 1,4- (3-D-glucan cellobiohydrolase (EC 3.2.1.91), endo- (3-1,4-D-glucan-4- glucanohydrolase (EC 3.2.1.4), and ß-glucosidase (EC 3.2.1.21). Cellulase enzymatic activity (expressed as endoglucanase units or CMCU) is typically determined by incubating an enzyme with carboxymethylcellulose (CMC) at pH 7.5 for 20 min, after which the formation of reducing sugars is determined using the p-hydroxybenzoic acid- hydrazide (PHBAH) rection (Lever, 1972, Anal. Biochem. :47 273-279, with the modification that 5g potassium sodium tartrate is added in addition to 1.5 g of PHBAH).

Enzymes having a low affinity for cellulose, also referred to as"low-affinity cellulases", may be identifie using, for example, the method described in Example 4 below, which involves incubation of the enzyme with Avicel to allow binding, followed by elution and detection of bound enzyme. Typically, an enzyme having a low affinity for cellulose will not exhibit binding to Avicel in this assay. The use of enzymes having higher affinity for cellulose is disadvantageous in a continuous or semi-

continuous apparats, because it results in (a) non-uniform adsorption of enzyme to the fabric and (b) loss of enzyme from the bulk solution because of adsorption to the fabric.

A cellulase having low affinity for cellulose generally lacks a functional cellulose-binding domain (CBD), either intrinsically or subsequent to modification of the cellulase sequence. CBDs are peptide sequences that confer high-affinity binding to cellulose, including, without limitation, sequences defined by Peter Tomme et al. in "Cellulose-Binding Domains: Classification and Properties"in Enzymatic Degradation of Insoluble Carbohydrates, John N. Saddler and Michael H. Penner (Eds.), ACS Symposium Series, No. 618,1996. Tomme et al. classifie more than 120 cellulose- binding domains into ten families (designated I-X), and identifie CBDs in various enzymes such as cellulases, xylanases, mannanases, arabinofuranosidases, acetyl esterases and chitinases, as well as in non-hydrolytic polysaccharide-binding proteins.

Low-affinity cellulases according to the present invention may either lack a CBD sequence entirely, or may contain a residual CBD sequence that has been modifie to destroy its cellulose-binding activity, by deletion, addition, and/or substitution of one or more residues or by any chemical or enzymatic modification of the intact protein; such a modifie sequence is also referred to as a non-functional CBD.

According to the invention, a fabric that has been contacte with a low-affinity cellulase is also exposed to high temperatures. Accordingly, the cellulases used in practicing the invention are preferably thermostable, i. e., exhibit optimal cellulase enzymatic activity at a temperature of at least about 55°C, preferably at least about 65°C, more preferably at least about 75°C and most preferably at least about 85°C.

Any low-affinity cellulase may be used in practicing the invention, so long as it exhibits at least about 20% of its maximal enzymatic activity at a temperatures above about 65°C. Preferably, the cellulase exhibits at least about 50% of its maximal activity at a temperature of about 65°C.

Non-limiting examples of cellulases useful in practicing the present invention include the cellulase from Pyrococcus whose sequence is depicted in SEQ ID NO: 1 and the cellulase from Dictyoglomus whose sequence is depicted in SEQ ID NO: 2. Other

suitable cellulases inclue, without limitation, cellulases derived from the following thermophilic cellulases, which have been modifie if necessary to reduce their affinity for cellulose: ß-glucosidase from Pyrococcus furiosus (Kengen et al., 1993, Eur. J. Biochem. 213: 305); exoglucanase from Thermotoga sp. (Ruttersmith et al., 1991, Biochem. J. 277: 887); cellulases from Thermotoga maritima (Bronnenmeier et al, 1995, Appl. Environ. Microbiol. 61: 1399; Microbiology 142: 2532,1996); ß- glucosidase from Thermotoga maritima (Gabelsberger et al., 1993, FEMS Microbiol.

Lett. 109: 131); endoglucanase B from Thermotoga neapolitania (Bok et al., 1994, ACS Symp. Ser. 566: 54); endoglucanase from Archebacteria (WO 97/44361); endoglucanase from Acidothermus cellulolyticus (WO 96/02551); cellulase from Rhodothermus marinus (Hreggvidsson et al., 1996, Environ. Microbiol. 62: 3047); and an exocellulase/endocellulase from Caldocellum saccharolyticum (Saul, Nuc. Acids Res.

17:439,1989).

The cellulases may be obtained from their cell of origin or from a recombinant organism that has been programme to synthesize the cellulase from a heterologous gene. Preferably, the cellulases are monocomponent enzymes, i. e., are single polypeptides having a defined enzymatic activity that are not synthesized as part of a multicomponent complex exhibiting multiple enzymatic activities. The cellulases may be recovered by conventional procedures including, but not limited to, centrifugation, filtration, spray-drying, evaporation, or precipitation. As used herein,"purified"or "isolated"cellulase is cellulase that has been treated to remove non-cellulase material derived from the cell in which it was synthesized that could interfere with its enzymatic activity. If the cellulase is secreted into the culture medium, purification may comprise separating the culture medium from the biomass by centrifugation, filtration, or precipitation, using conventional methods. Alternatively, the cellulase may be released from the host cell by cell disruption and separation of the biomass. In some cases, further purification may be achieved by conventional protein purification methods, including without limitation ammonium sulfate precipitation; acid or chaotrope extraction; ion-exchange, molecular sieve, and hydrophobic chromatography, including FPLC and HPLC; preparative isoelectric focusing; and preparative polyacrylamide gel

electrophoresis. Alternatively, purification may be achieved using affinity chromatography, including immunoaffinity chromatography. For example, hybrid recombinant cellulases may be used having an additional amino acid sequence that serves as an aff'nity"tag", which facilitates purification using an appropriate solid- phase matrix.

Other Components In some embodiments of the invention, the bulk solution containing the low- affinity cellulase further comprises other components, including without limitation other enzymes, as well as one or more of surfactants, bleaching agents, antifoaming agents, builder systems, and the like, that enhance the biopolishing process and/or provide superior effects related to, e. g., dyeability and/or wettability. The aqueous solution may also contain dyeing agents.

Enzymes suitable for use in the present invention include without limitation: Pectin-digesting enzymes: Suitable pectin-digesting enzymes (some of which are identifie by their Enzyme Classification numbers in accordance with the Recommendations (1992) of the International Union of Biochemistry and Molecular Biology (IUBMB)) inclue, without limitation, pectin-degrading enzymes such as pectate lyase, pectin lyase, pectin methyl esterase, polygalacturonase 2.1.15),(3. and rhamnogalacturonase (WO 92/19728).

Hemicellulases: Suitable hemicellulases include without limitation endo- arabinanase 2.1.99,(3. Rombouts et al., Carb. Polymers :9 25,1988), <BR> <BR> <BR> <BR> arabinofuranosidase, endo-ß-1,4-galactanase, endo-xylanase 2.1.8),(3. mannanase, and xyloglucanase.

Amylases: Suitable amylases include a-amylases (a-1,4 glucan-4-glucanohydrolase, EC 2.1.1),3. including, without limitation, Bacillus a-amylases (which in the present context are termed"Termamyl-like a-amylases"), including B. licheniformis, B. amyloliquefaciens, and B. stearothermophilus a-amylase.

Commercially available Termamyl-like B. licheniformis a-amylases are Optitherms and Takatherm (available from Solvay), Maxamyls (available from Gist-

brocades/Genencor), Spezym AA (available from Genencor), and Keistase (available from Daiwa). Non-Termamyl-like a-amylase inclue, without limitation, members of the Fungamyl-like a-amylase family.

Protases: Suitable protases include those of animal, vegetable or microbial origin, preferably of microbial origin. The protase may be a serine protase or a metalloprotease, preferably an alkaline microbial protase or a trypsin-like protase.

Examples of protases include aminopeptidases, including prolyl aminopeptidase (3.4.11.5), X-pro aminopeptidase (3.4.11.9), bacterial leucyl aminopeptidase (3.4.11.10), thermophilic aminopeptidase (3.4.11.12), lysyl aminopeptidase (3.4.11.15), tryptophanyl aminopeptidase (3.4.11.17), and methionyl aminopeptidase (3.4.11.18); serine endopeptidases, including chymotrypsin (3.4.21.1), trypsin (3.4.21.4), cucumisin (3.4.21.25), brachyurin (3.4.21.32), cerevisin (3.4.21.48) and subtilisin (3.4.21.62); cystine endopeptidases, including papain (3.4.22.2), ficain (3.4.22.3), chymopapain (3.4.22.6), asclepain (3.4.22.7), actinidain (3.4.22.14), caricain (3.4.22.30) and ananain (3.4.22.31); aspartic endopeptidases, including pepsin A (3.4.23.1), Aspergillopepsin I (3.4.23.18), Penicillopepsin (3.4.23.20) and Saccharopepsin (3.4.23.25); and metalloendopeptidases, including Bacillolysin (3.4.24.28).

Non-limiting examples of subtilisins include subtilisin BPN', subtilisin amylosacchariticus, subtilisin 168, subtilisin mesentericopeptidase, subtilisin Carlsberg, subtilisin DY, subtilisin 309, subtilisin 147, thermitase, aqualysin, Bacillus PB92 protase, proteinase K, protase TW7, and protase TW3.

Commercially available protases include AlcalaseT"', SavinaseT"', PrimaseT"', DuralaseT"', EsperaseT"', and KannaseT"^ (Novo Nordisk A/S), MaxataseT"', MaxacalTM, MaxapemT"", ProperaseTM, PurafectT"', Purafect OxPTM, FN2T"', and FN3TM (Genencor International Inc.).

Also contemplated for use in the present invention are protase variants, such as those disclosed in EP 130.756 (Genentech), EP 214.435 (Henkel), WO 87/04461 (Amgen), WO 87/05050 (Genex), EP 251.446 (Genencor), EP 260.105 (Genencor), Thomas et al., (1985), Nature :318 375-376, Thomas et al., (1987), J. Mol. Biol. , 193,

pp. 803-813, Russel et al., (1987), Nature 328: 496-500, WO 88/08028 (Genex), WO 88/08033 (Amgen), WO 89/06279 (Nove Nordisk A/S), WO 91/00345 (Nove Nordisk A/S), EP 525 610 (Solvay) and WO 94/02618 (Gist-Brocades N. V.).

The activity of protases can be determined as described in"Methods of Enzymatic Analysis", third edition, 1984, Verlag Chemie, Weinheim, vol. 5.

Lipases: Suitable lipases (also termed carboxylic ester hydrolases) include those of bacterial or fungal origin, including triacylglycerol lipases (3.1.1.3) and Phospholipase A2. (3.1.1.4.). Lipases for use in the present invention inclue, without limitation, lipases from Humicola (synonym Thermomyces), such as from H. lanuginosa (T. lanuginosus) as described in EP 258 068 and EP 305 216 or from H. insolens as described in WO 96/13580; a Pseudomonas lipase, such as from P. alcaligenes or P. pseudoalcaligenes (EP 218 272), P. cepacia (EP 331 376), P. stutzeri (GB 1,372,034), P. fluorescens, Pseudomonas sp. strain SD 705 (WO 95/06720 and WO 96/27002), P. wisconsinensis (WO 96/12012); a Bacillus lipase, such as from B. subtils (Dartois et al., Biochem. Biophys. Acta, 1131: 253-360,1993), B. stearothermophilus (JP 64/744992) or B. pumilus (WO 91/16422). Other examples are lipase variants such as those described in WO 92/05249, WO 94/01541, EP 407 225, EP 260 105, WO 95/35381, WO 96/00292, WO 95/30744, WO 94/25578, WO 95/14783, WO 95/22615, WO 97/04079 and WO 97/07202. Preferred commercially available lipase enzymes include Lipolase and Lipolase UltraT"', LipozymeTM, PalataseTM, NovozymTM435, and LecitaseTM (all available from Novo Nordisk A/S).

The activity of the lipase can be determined as described in"Methods of Enzymatic Analysis", Third Edition, 1984, Verlag Chemie, Weinhein, vol. 4.

Preferably, the enzymes are derived from alkalophilic microorganisms and/or exhibait enzymatic activity at elevated temperatures. The enzymes may be isolated from their cell of origin or may be recombinantly produced, and may be chemically or genetically modifie. Typically, the enzymes are incorporated in the aqueous solution at a level of from about 0.0001% to about 1 % of enzyme protein by weight of the composition, more preferably from about 0.001 % to about 0.5 % and most preferably from 0.01% to 0.2 %. It will be understood that the amount of enzymatic activity units

for each additional enzyme to used in the methods of the present invention in conjunction with a particular cellulase can be easily determined using conventional assays.

Surfactants suitable for use in practicing the present invention inclue, without limitation, nonionic (U. S. Patent No. 4,565,647); anionic; cationic; and zwitterionic surfactants (U. S. Patent No. 3,929,678); which are typically present at a concentration of between about 0.002% to about 3% by weight, preferably from about 0.02% to about 2% by weight. Anionic surfactants inclue, without limitation, linear alkylben- zenesulfonate, a-olefinsulfonate, alkyl sulfate (fatty alcohol sulfate), alcohol ethoxysulfate, secondary alkanesulfonate, alpha-sulfo fatty acid methyl ester, alkyl-or alkenylsuccinic acid, and soap. Non-ionic surfactants inclue, without limitation, alcohol ethoxylate, nonylphenol ethoxylate, alkylpolyglycoside, alkyldimethylamine- oxide, ethoxylated fatty acid monoethanolamide, fatty acid monoethanolamide, polyhydroxy alkyl fatty acid amide, and N-acyl N-alkyl derivatives of glucosamine ("glucamides").

Builder systems inclue, without limitation, aluminosilicates, silicates, polycarboxylates and fatty acids, chelating agents such as ethylenediamine tetraacetate, aminopolyphosphonates, particularly ethylenediamine tetramethylene phosphonic acid, and diethylene triamine pentamethylenephosphonic acid, which are included at a concentration of between about 5% to 80% by weight, preferably between about 5% and about 30% by weight.

Bleaching systems may comprise an oxidizing agent such as hydrogen peroxide, perborate, peracetate, or percarbonate, which may be combine with a peracid-forming bleach activator such as tetraacetylethylenediamine or nonanoyloxybenzenesulfonate.

Alternatively, the bleaching system may comprise peroxyacids of, e. g., the amide, imide, or sulfone type.

Antifoam agents include without limitation silicones (U. S. Patent No.

3,933,672; DC-544 (Dow Corning), which are typically included at a concentration of between about 0.01 % and about 1 % by weight.

The compositions may also contain soil-suspending agents, soil-releasing agents, optical brighteners, abrasives, and/or bactericides, as are conventionally known in the art.

Dyeing agents including, without limitation, dyes disclosed in Shore (ed.), Cellulosic Dyeing, 1995 (Society of Dyers and Colorists, Alden Press, Oxford).<BR> <BR> <P>Biopolishing Methods The present invention provides methods for biopolishing fabric which comprise (a) coiitacting a cellulosic fabric, preferably in a continuos or semi-continuous apparats, with an aqueous solution comprising at least a low-affinity cellulase and (b) subjecting the contacte fabric to a high temperature. The contacting step involves exposing the fabric relatively briefly (typically, for less than 5 min) to a bulk solution containing the enzyme, after which the fabric may be padded to remove excess solution. This results in a wet pick-up (expressed as weight of solution: weight of fabric x 100) of between about 50 and about 200%, preferably between about 50 and about 130%. The contacting and subjecting steps may be performed simultaneously (i. e., by contacting the fabric with the bulk solution while heating) or sequentially (i. e., by first contacting the fabric with the bulk solution; optionally removing excess solution; and, subsequently, subjecting the wetted fabric to high temperature).

To achieve effective biopolishing, the concentration of enzyme in the aqueous solution (CMCU/ml), the temperature to which the fabric is subjected, and the total incubation time, will vary, depending on: (i) the nature of the fabric; (ii) the particular low-affinity cellulase used; (iii) the pH of the solution; (iv) the time during which the fabric is contacte with the bulk solution; and (v) the presence of other components in the aqueous solution.

Determination of suitable enzyme concentration to be used, as well as optimization of other variables, can be achieved using only routine experimentation by

establishing a matrix of conditions and testing different points in the matrix. For example, the enzyme concentration, the temperature at which the contacting occurs, and the time of contact can be varied, after which the resulting fiber or textile is evaluated for (a) one or more biopolished properties, such as, e. g., fabric handle, appearance, or pilling resistance, and, optionally, (b) potential loss in fabric strength and/or weight.

Fabric handle and appearance are evaluated by panel testing, using a rating of 1- 3 (worst to best).

Pilling can be measured using any conventional method, such as, e. g., according to American Society for Testing and Materials protocol ASTM D 4970-89, using a Martindale Abrasion and Pilling Tester (James H. Heal & Co, UK). In this method, pilling is evaluated visually on a scale of 1 to 5, where 1 signifies severe pilling and 5 signifies no pilling.

Fabric strength is measured using any conventional method, such as, e. g., according to ASTM protocol D 3786-87, using a Mullen Burst tester (Model C, B. F.

Perkins, Chicopee MA).

In practicing the invention, conditions are selected in which one or more biopolished properties, particularly pilling, show improvements over untreated controls, but in which fabric strength loss is minimal. Preferably, a pilling note increase of at least about 0.25 is observe, more preferably at least about 0.5, and most preferably at least about 1.0. Preferably, fabric strength loss is less than about 20%, more preferably less than about 10 %, and most preferably less than about 5 %.

Typically, the bulk solution contains a low-affinity cellulase at a concentration of less than about 200 CMCU/ml, more preferably less than about 100 CMCU/ml, and most preferably less than about 50 CMCU/ml; at a temperature of at least about 65°C, preferably at least about 75°C, and most preferably at least about 85°C; and at a pH of between about 4 and 12, preferably between about 5 and 10, and most preferably between about 7 and 10.

Combination methods: The present invention also encompasses combination methods in which biopolishing is carried out simultaneously with scouring and/or

dyeing. In these embodiments, the aqueous bulk solution also contains other components, including without limitation the enzymes disclosed herein, as well as other components, such as, e. g., dyes (including without limitation reactive dyes, direct dyes, sulphur dyes, and vat dyes) and dye auxiliaries. See, Shore (ed.), Cellulosic Dyeing, 1995 (Society of Dyers and Colorists, Alden Press, Oxford). The contacte fabric is then subjected to a high temperature, which results in simultaneous dyeing or scouring and biopolishing.

The following examples are intended as non-limiting illustrations of the present invention.

Example 1: Biopolishing Using Dictyoglomus Cellulase The following expriment was performed to evaluate the biopolishing capability of Dictyoglomus cellulase in a continuous apparats.

Methods : The fabric used was Knitted Fabric 460 (Test Fabrics Inc.), which is 100% cotton bleached interlock. The fabric was cut into 20x30 cm pieces weighing about 12.5 g each. The weight of each swatch was determined after conditioning for at least 24 hours at 65 2 % relative humidity and 21 2°C (70 t 3 °F).

The cellulase comprise the catalytic domain from Dictyoglomus cellulase (whose sequence is shown in SEQ ID NO: 2) which was formulated in 15 mM sodium phosphate. The pH and enzyme concentration were as shown in Table 1 below.

Swatches were contacte with enzyme solutions for less than 45 seconds and then padded through a pad, after which they were weighed and hung immediately in a Mathis steam range (Type PSA-HTF) (Werner Mathis USA Inc. Concord, NC). The percentage of solution on fabric (% wet pick-up) and ratio of cellulase activity to fabric are shown in Table 1. Fabric swatches were treated at 90°C and 100% relative humidity for 90 minutes. All swatches were then transferred and rinsed in de-ionized water for at least 5 minutes, after which they were air dried. Finally, the swatches

were conditioned at 65 t 2 % relative humidity and 21 t 2°C (70 3 ° F) temperature for at least 24 hours before evaluation.

Fabric strength was measured on Mullen Burst tester model C according to ASTM D3786-87: Standard Test Method for Hydraulic Bursting Strength of Knitted Goods and Nonwoven Fabrics-Diaphragm Bursting Strength Tester Method. The results are presented as the average of at least 8 measurements. Pilling note was measured according to ASTM D 4970-89: Standard Test Method for Pilling Resistance and Other Related Surface Changes of Textiles Fabrics (Martindale Pressure Tester Method). After 500 revolutions, pilling on the fabric was evaluated visually against a standard scale 1 to 5, where 1 indicates very severe pilling and 5 indicates no pilling.

The results are presented as the average of at least two measurements.

Results : The results are shown in Table 1 below and in Figure 1. As the concentration of enzyme increased, a corresponding increase in pilling note was observe. The increase in pilling resistance is greater at pH 8.1 than at pH 6.0. Under the indicated conditions of enzyme concentration and pH, the method of the invention results in minimal fabric strength loss (less than 5% loss at pH 6.0 and no detectable loss at pH 8.1). An even pilling on the surface also indicated that the fabric had been exposed uniformly to the cellulase.

These results demonstrate that biopolishing of cotton fabric with Digtyoglomus cellulase improves fabric pilling resistance significantly without detectable strength loss.

Table 1 SOLUTION CELLULASE WET CELLULASE STRENGTH PILLIN PH SOLUTION PICK-UP ACTIVITY LOSS NOTE (CMCU/ML) (% W/W) (CMCU/G (%) (500 FABRIC) REV) 6 0 125 0 0 1. 5 3 6 4.8 130 6.2 2.4 2 5 6 9.7 137 13.3 3.5 2.5 2 8.1 0 128 0 0 2 4 8.1 4.8 131 6.3 0 2.75 6 8.1 9.7 137 13.3 0 3

Example :2 Biopolishing Using Pvrococcus Cellulase The following expriment was performed to evaluate the biopolishing capability of Dictyoglomus cellulase in a continuous apparats.

Biopolishing was carried out essentially as described in Example 1, except that the buffer used consiste of 9.53 g sodium tetraborate decahydrate dissolve in 2.5 1 deionized water and adjusted to pH 9.2, and the cellulase was derived from Pyrococcus (whose sequence is depicted in SEQ ID NO: 1).

Methods: Swatches were padded and treated as described in Example 1. The fabric wet pick-up was 94%. The fabric was treated for 90 min at pH 9.2,90°C, and relative humidity 100%. The rinsing, drying, evaluating procedures were the same as in Example 1 except that pilling note was evaluated after 125 revolutions.

Results: No statistically significant strength loss was detected for all cellulase-treated swatches when compare with controls that were not exposed to enzymes. On the other hand, pilling note increases as enzyme activity increases (Figure 2). These results indicated that Pyroccocus cellulases are useful for biopolishing, while causing

little fabric strength loss in a Pad Steamer apparats. A better appearance and fabric handle were also achieved.

Example 3: Combination Treatments The following experiments were performed to evaluate the methods of the present invention in combine scouring and biopolishing.

Methods : The fabric used was Fabric 4600, which is an unscoured and unbleached 100% cotton fabric. Fabric preparation and buffer were the same as described in Example 2 above.

The bulk solution contained: (a) The Pyroccucus cellulase described in Exemple 2 above, at a concentration of 6.12 CMCU/ml and 4.9 CMCU/g fabric; and (b) thermostable pectate lyase at a concentration of 1.93 mv-mol/ml/min. Swatches were padded and treated as described in Example 1. The fabric wet pick-up was 80%.

Treatment conditions were pH 9.2,90°C, relative humidity (RH) 100%, and treatment was for 90 min.

The rinsing, drying, evaluating procedures are the same as described in Example 1 above. Wetting speed was evaluated according to the AATCC test method.

A water drop from 1 cm high burette was allowed to fall to a taut surface of fabric specimen. The time for water disappearance on the fabric surface was recorde as wetting time. Eight measurements on each specimen were carried out and averaged.

Results : Fabric pilling resistance improved after either cellulase treatment or combine treatment with cellulase and pectinase (Table 2). Furthermore, the average wetting time also decreased significantly relative to non-enzyme-treated controls (Table 3). These results indicated that the methods of the invention can be used in combine biopolishing and scouring.

Table 2 Pilling Note (125 rev.) non-enzyme 2 Cellulase 2.5 Pectinase 2 Cell+ Pect. 2.5 Table 3 wetting time (second) 1 2 3 4 5 6 7 8 9 Average non-enzyme 7 50 115 249 >300 >300 >300 >300 >300 >300 Cellulase 64 40 40 46 164 124 214 182 109 Pectinase 61 70 65 64 94 96 95 64 104 79 Cell+ Pect. 37 40 39 30 28 22 28 28 32 Example 4: Identification of Low-Affinitv Cellulases The following method is used to measure the affinity of a polypeptide for cellulose, in order to identify low-affinity cellulases.

200 Ill of a 1 mg/ml enzyme solution is mixed with 200 pl of a 10% (w/v) Avicel suspension, which is made up in 0. 1M sodium phosphate buffer, pH 7.5, and mixed for 15 min. The mixture is incubated for lh at 4°C, after which it is subjected to centrifugation for 5 min at 5000 rpm in a microfuge. The supernatant is removed, and the Avicel is washed with 1 ml of buffer and re-pelleted. Finally, the Avicel pellet is resuspended in SDS-PAGE loading buffer and incubated at 95°C for 2 min. After centrifugation for 5 min at 5000 rpm, the supernatant is recovered and loaded on a 4-20% gradient acrylamide SDS gel (Novex), and electrophoresis is performed in an Xcell mini- cell (Novex). Electrophoresis and staining are performed according to the manufacturer's instructions.

Using this method, low-affinity cellulases are identifie as cellulases that do not result in a detectable band in SDS-PAGE using Coomassie Blue staining.

All patents, patent applications, and literature references referred to herein are hereby incorporated by reference in their entirety.

Many variations of the present invention will suggest themselves to those skilled in the art in light of the above detailed description. Such obvious variations are within the full intended scope of the appende claims