BACKGROUND OF THE INVENTION This invention relates to receptors, particularly receptors involved in neurotransmission.

Substance P (SP) is one member of a family of peptide neurotransmitters known as tachykinins (von Euler et al., J. Physiol . 21:74, 1931; Chang et al., J. Biol . Chem . 245:4784, 1970).

Molecular characterization has revealed that tachykinins are transcribed from alternatively-spliced mRNA molecules (termed, a , β and mRNAs) and are translated as precursor molecules, termed preprotachykinins (Nawa et al., Nature 306:32, 1983; Kawaguchi et al., Biochem . Biophyε . Res . Commun . 139:1040, 1986; Krause et al., Proc . Natl. Acad . Sci, USA 84.J881, 1987). Specifically, the β and η messages encode preprotachykinins which include substance P and another tachykinin, neurokinin A; the β mRNA molecule encodes an amino-terminally extended form of neurokinin A, termed neuropeptide K or NpK; and the α form encodes only substance P. Mature tachykinin molecules are produced from the preprotachykinins by proteolytic processing. Structurally, the tachykinin family shares the COOH- terminal protein sequence: Phe-X-Gly-Leu-Met-NH ₂ (SEQ ID N0.:1), where X is Phe, Tyr, Val, or lie. Substance P, in particular, is of amino acid sequence: Arg-Pro-Lys- Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH ₂ (SEQ ID NO.:2). Three classes of tachykinin receptors have been identified by bioassay and radioligand binding (Martling et al., Life Sci . 4.0:1633, 1987; Buck et al., Science 226:987, 1984; Burcher et al., J . Pharmacol . Exp . Ther . 236:819, 1986). Analysis of these receptors has revealed a COOH-terminal consensus sequence of the receptors which

controls biological activity and divergent amino-terminal sequences which determine receptor affinity. The result of such an arrangement is that each tachykinin recognizes each of the three receptor types, but with varying avidity. In particular, the NK-1 receptor preferentially binds substance P; the NK-2 receptor preferentially binds neurokinin A; and the NK-3 receptor preferentially recognizes neurokinin B (another tachykinin, remote from neurokinin A or substance P) . Synthetic tachykinin analogs designed to act as competitive inhibitors exhibit relative selectivity for each of the three neurokinin receptors ( ormser et al., BMBO J. .5:2805, 1986; Cavinikas et al., Eur. J. Pharmacol . 77:205, 1982; Regoli et al., Tachykinin Antagonists , eds. R. Hakanson and F. Sundler) pp. 277-287, Elsevier Science Publisher B. V., Amsterdam, 1985) .

Tachykinins, in general, have been found to display a wide tissue distribution and to be involved in numerous physiological activities (Lee et al., Eur. J. Pharmacol . 130:209, 1986; Lundberg et al. , Aσta Physiol . Scand. 119:243. 1983; Nicoll et al., Ann . Rev. Neurosci . 3.:227, 1980; Pernow, Pharmacol . Rev. 3_5:85, 1983; Maggio, Ann. .Rev. Neurosci. 1_1:13, 1988). Such activities include vasodilation (Pernow and Rosell, Acta . Physiol . Scand. 3.5139, 1975), smooth muscle contraction (Pernow, Acta Physiol . Scand. 93 Suppl. 105:1, 1953), stimulation of salivary secretion (Vogler et al., N. Y. Acad. Sci . 104:378. 1963; Leeman and Hammerschlag, Endocrinology 1:803, 1967), transmission of painful stimuli from the peripheral nervous system, and interactions with other neurotransmitters in the brain (Nicoll et al., Ann . Rev. Neurosci . 3.:227, 1980; Maggio, Ann . Rev. Neurosci . 11:13, 1988; Nilsson et al. , Med. Biol . 52:424, 1974; Hokfelt et al., Science 190:889, 1975; Mantyh et al., Nature 309:795, 1984; omack et al., Nature 334:351. 1988).

Substance P has also been found to play a role in the activation of cells of the immune system, including mononuclear leukocytes (e.g., monocytes and lymphocytes) and polymorphonuclear leukocytes (Payan et al., J ^* . Clin . .Invest. 7_4Jl532, 1984; Hartung et al., J ^* . Immunol. 13J5:3856, 1986; Lotz et al., Science 235:893, 1987; Perianin et al., Biochem . Biophys . Res . Commun . 161:520, 1989) .

SUMMARY OF THE INVENTION In one aspect, the invention generally features human recombinant NK-1 receptor or a fragment thereof. Preferably, the receptor includes an amino acid sequence substantially identical to the amino acid sequence shown in Fig. 1 (SEQ ID N0.:3). By a "substantially identical" amino acid sequence is meant an amino acid sequence which differs only by conservative amino acid substitutions, for example, substitution of one amino acid for another of the same class (e.g., valine for glycine, arginine for lysine, etc.) or by one or more non-conservative amino acid substitutions, deletions, or insertions located at positions of the amino acid sequence which do not destroy the biological activity of the receptor. Such equivalent growth factors can be isolated by extraction from the tissues or cells of any animal which naturally produce such a receptor or which can be induced to do so, using the methods described below, or their equivalent; or can be isolated by chemical synthesis; or can be isolated by standard techniques of recombinant DNA technology, e.g., by isolation of cDNA or genomic DNA encoding such a receptor.

In a related aspect, the invention features a substantially isolated polypeptide which is a fragment of a human NK-1 receptor and includes an extracellular domain capable of binding substance P neurotransmitter. By a "polypeptide" is meant any chain of amino acids,

regardless of length or post-translational modification (e.g., glycosylation) . A "substantially isolated polypeptide" is one which is substantially free of other proteins, carbohydrates and lipids with which it is naturally associated. By an "extracellular domain" is meant any portion of the protein (in this case, the human NK-1 receptor) which is exposed on the outer surface of a receptor-bearing cell, and which contains significant structural information to participate in or define specific binding.

In preferred embodiments, the polypeptide is selected from the group including:

(a) MDNVLPVDSDLSPNISTNTSEPNQFVQPA Q (SEQ ID NO.:4; amino acid residues 1 to 31 of Fig. 1; SEQ ID NO.:3); (b) FTYAVHNE YYG (SEQ ID NO.:5; amino acid residues 90 to 101 of Fig. l; SEQ ID N0.:3);

(c) TTETMPSRWCMIEWPEHPNKIYEKVYH (SEQ ID NO.:6; amino acid residues 170 to 197 of Fig. 1; SEQ ID NO.:3);

(d) PYINPDLYLKKFIQQVY (SEQ ID N0.:7; amino acid residues 271 to 287 of Fig. 1; SEQ ID N0.:3); and (e) fragments or analogues of (a)-(d) which are capable of binding human NK-1 neurotransmitter. Preferably, such a polypeptide is a recombinant polypeptide.

In other related aspects, the invention features purified DNA which encodes a receptor (or fragment thereof) or a polypeptide described above; vectors which contain such DNA and are capable of directing expression of the protein encoded by the DNA in a vector-containing cell; and cells containing such vectors (preferably eukaryotic cells, e.g., mammalian cells). By "purified DNA" is meant a DNA molecule which encodes the human NK- 1 receptor (or an appropriate receptor or analog) , but which is free of the genes that, in the naturally- occurring genome of the organism from which the DNA of

the invention is derived, flank the gene encoding the NK- 1 receptor.

The expression vectors or vector-containing cells of the invention can be used in a method of the invention to produce human NK-1 receptor and the polypeptides described above. The method involves providing a cell transformed with DNA encoding the NK-1 receptor or a fragment thereof positioned for expression in the cell; culturing the transformed cell under conditions for expressing the DNA; and isolating the recombinant human NK-1 receptor protein. By "transformed cell" is meant a cell into which (or into an ancestor of which) has been introduced, by means of genetic engineering, a DNA molecule encoding the human NK-1 receptor (or a fragment or analog, thereof) . Such a DNA molecule is "positioned for expression" meaning that the DNA molecule is positioned adjacent to a DNA sequence which directs transcription and translation of the sequence (i.e., facilitates the production of the NK-1 receptor protein, or fragment or analog, thereof) .

In yet another aspect, the invention features purified antibody which binds preferentially to the human NK-1 receptor (or a fragment thereof) or a polypeptide described above. By "purified antibody" is meant one which is sufficiently free of other proteins, carbohydrates, and lipids with which it is naturally associated to permit therapeutic administration. Such an antibody "preferentially binds" to a human NK-l receptor (or fragment or analog, thereof), i.e., does not substantially recognize and bind to other antigenically- unrelated molecules. Preferably, the antibody neutralizes the biological activity in vivo of the protein to which it binds. By "biological activity" is meant the ability of the NK-1 receptor to bind substance P and signal the appropriate cascade of biological

events. By "neutralize" is meant to partially or completely block (e.g. , the biological activity of the human NK-1 receptor) .

In other aspects, the receptor, polypeptides, or antibodies described above are used as the active ingredient of therapeutic compositions. In such therapeutic compositions, the active ingredient may be formulated with a physiologically-acceptable carrier or anchored within the membrane of a cell. These therapeutic compositions are used in methods of reducing pain, treating inflammatory diseases (e.g. , arthritis or asthma) , treating mental illnesses (e.g. , schizophrenia) , and treating physiological responses to stress. ("Stress" as used herein means the reaction of the body to forces of a deleterious nature, infections, and various abnormal states that tend to disturb homeostasis) . The methods involve administering to a mammal the therapeutic composition in a dosage effective to antagonize an interaction between substance P neurotransmitter and an NK-1 receptor.

Finally, the invention features a method of screening candidate compounds for their ability to antagonize interaction between substance P neurotransmitter and an NK-1 receptor. The method involves: a) mixing a candidate antagonist compound with a first compound which includes a recombinant NK-1 receptor (or fragment) or a polypeptide or an antibody described above on the one hand and with a second compound which includes substance P on the other hand; b) determining whether the first and second compounds bind; and c) identifying antagonistic compounds as those which interfere with the binding of the first compound to the second compound. By an "antagonist" is meant a molecule which inhibits a particular activity, in this case, the ability of substance P to interact with the NK-

1 receptor and/or to trigger the biological events resulting from such an interaction.

The proteins of the instant invention are involved in a wide range of physiological activities and therefore are useful to treat or, alternatively, to develop therapeutics to treat a variety of disorders (e.g., molecules which antagonize a substance P: NK-1 receptor interaction) . The disorders to be treated include pain, particularly chronic pain; diseases arising from inappropriate inflammatory responses, such as arthritis and asthma; and mental-health disorders, such as schizophrenia, anxiety, stress, and the like. Preferred therapeutics include antagonists, e.g., peptide fragments, antibodies, or drugs, which block substance P or NK-1 receptor function by interfering with the neurotransmitter:receptor interaction. Because the receptor component may now be produced by recombinant techniques and because candidate antagonists may be screened in vitro, the instant invention provides a simple and rapid approach to the identification of useful therapeutics. Once identified, a peptide- or antibody- based therapeutic may also be produced, in large quantity and inexpensively, using recombinant and molecular biological techniques. Other features and advantages of the invention will be apparent from the following description of the preferred embodiments thereof, and from the claims.

DETAILED DESCRIPTION The drawings will first briefly be described. DRAWINGS

FIG. 1 shows the complete nucleic acid and deduced amino acid sequence of a cDNA encoding the human NK-1 receptor gene (i.e., SEQ ID NO.:3). CLONING OF THE HUMAN NK-1 RECEPTOR GENE The human NK-1 receptor was cloned as follows.

Human IM-9 lymphocyte cells (ATCC Accession No. CCL 159) were cultured by standard techniques. RNA was extracted from approximately 10 cells by first freezing in liquid nitrogen and then extracting with guanidium thiocyanate (by the methods of Ullrich et al.. Science 196:1313. 1977; and Chirgwin et al., Biochemistry 24.:5294, 1979), and centrifuging through cesium chloride by the method of Gilsin et al. (Biochemistry 13:2633, 1974). Poly(A) ⁺ RNA was then isolated by passing the material over oligo(dT)-Sepharose (Pharmacia, Piscataway, NJ) by the method of Aviv and Leder (Proc. Natl . Acad . Sci . USA 69 _. :14087, 1972) and transcribed into cDNA by the method of Gubler and Hoffman (Gene (Amεt . ) 15:263, 1983). Synthetic oligonucleotide primers were designed based on the cDNA sequence reported for the rat NK-1 receptor (Nakanishi et al., J. Biol . chem. 264:17649. 1989) . These primers extended from nucleotide 85 to 105 (i.e., the sense primer) and from nucleotide 538-558 (i.e., the antisense primer); each included EcoRI restriction site sequences at their 5' ends. Such primers were, respectively, of sequence:

5'-AATGAATTCACCTGGCAAATCGTTCTTTGG-3' (SEQ ID NO. :8) 5•-AATGAATTCCTCCGGCCACTCGATCATGCA-3' (SEQ ID NO. :9) The primers were synthesized by standard cyanoethyl phosphoramidite chemistry using an Applied Biosystems Model 318A DNA Synthesizer (Foster City, CA) .

Approximately 100 ng of human IM-9 lymphocyte cell cDNA was combined with 1 μg of each of the synthetic primers and recombinant Taq DNA polymerase (Perkin-Elmer, Norwalk, CT) . Twenty-five cycles of polymerase chain reaction were carried out by the method of Gerard et al. (J. Biol . Chem . 265:20455, 1990). Each cycle included: 1 min at 95°C, 2 min at 37°C, and 2 min at 72°C. This was followed by a final extension period of 7 min at 72°C. The PCR product, an approximately 500 base pair

fragment including nucleotides 85 to 555, was purified, following electrophoretic separation, using GeneClean (Bio 101, La Jolla, CA) . The fragment was digested with j_?σoRI, repurified as above following a second round of electrophoresis, and ligated to coRI-digested pBluescript SK+ (Stratagene, La Jolla, CA) . Clones were amplified in Escherichia coli XL-lblue (Stratagene, La Jolla, CA) , purified by centrifugation through cesium chloride (by the method of Birnbolm and Doly, Nucl . Acids Res . 2:1513, 1979), and subjected to double-stranded nucleic acid sequencing using DNA polymerase and [ ⁵ S]dATP by the method of the supplier (Sequenase, United States Biochemical Corporation, Cleveland, OH) . The fragment exhibited 90% sequence identity with the rat molecule. A human placental genomic DNA library, prepared by standard techniques, was screened by Southern blot analysis using the 500-bp NK-1 receptor fragment (isolated above) labelled with ³ P. Bacteriophage DNA was transferred to nitrocellulose filters (in duplicate) and hybridized with the probe for 16 h, at 42°C, in 5X SSC (0.75M NaCl, 0.075M sodium citrate)/50% formamide, containing 20mM Tris-HCl, pH 7.5, IX Denhardt's solution, 10% dextran sulfate, and 0.1% SDS. Filters were washed three times, for 10 min each, in 2X SSC/0.1% SDS, at 22°C; followed by one wash, for 30 min, in 0.2X SSC/0.1% SDS at 68°C; and exposed to X-ray film (Kodak X-Omat, Eastman Kodak, Rochester, NY) at -70°C. Three overlapping clones were obtained, which together constituted the entire NK-1 receptor gene. The NK-1- encoding fragments were subcloned into pBluescript

(Stratagene, La Jolla, CA) and sequenced by the method of double-stranded sequencing (as described above) .

A full-length human NK-2 receptor cDNA was cloned as follows.

Oligonucleotide primers corresponding to the putative 3' end and the putative 5' end of the cDNA were designed based on an analysis of the genomic NK-1 sequence and the identification of a putative 5* ATG site. Specifically, these primers corresponded to nucleotides -7 to +11 (i.e., the sense primer) and nucleotides 1190 to 1298 (i.e., the antisense primer) and each included a nested BamHI restriction site at its 5 ^• end. Primers were synthesized as described above. PCR reactions were carried out (also as described above) using about 100 ng of human IM-9 lymphocyte cDNA and 1 μg of each primer. The PCR amplification involved 5 cycles, each including 1 min. at 95°C , 1.5 min. at 45°C, and 3 min. at 72°C; followed by 25 cycles, each including 1 min. at 95°C, 1.5 min at 55°C, and 3 min. at 72°C; and a final extension of 7 min. at 72°C. Ten percent of the reaction mixture was subjected to secondary PCR, using the same primers and the same cycling conditions as were used for the initial reaction. The material obtained was purified by agarose electrophoresis, digested with BamHI, repurified by agarose electrophoresis, and ligated to BamHI-digested pBluescript SK+ (Stratagene; La Jolla, CA) . The PCR product was determined to be a fragment of approximately 1.2 kb. The cDNA clone was sequenced using the methods described above; its nucleic acid sequence and deduced amino acid sequence are shown in FIG. 1 (SEQ ID NO.:3) .

By comparison of the NK-1 receptor sequence with other members of the rhodopsin superfamily and by examination of the hydrophobic and hydrophilic regions of the molecule, extracellular and intracellular domains may be deduced. Extracellular domains include: amino acids 1 to 31 (SEQ ID N0.:4); amino acids 90 to 101 (SEQ ID NO.:5); amino acids 170 to 197 (SEQ ID N0.:6); and amino acids 271 to 287 (SEQ ID N0.:7). Intracellular domains

include: amino acids 60 to 67; amino acids 129 to 148; amino acids 220 to 248; and 310 to 407. POLYPEPTIDES ACCORDING TO THE INVENTION

Polypeptides according to the invention include the entire human NK-1 receptor as described in FIG. 1 (SEQ. ID. N0.:3). Alternatively, any analog or fragment of the NK-1 receptor capable of interacting with substance P is useful in the invention. Such an interaction may be readily assayed using any of a number of standard in vitro methods (see, e.g., Regoli et al., Tachykinin .Antagonists , eds. Hakanson and Sundler, Elsevier Science Publisher, Amsterdam, 1985) . In one particular assay, substance P is adhered to a microtiter plate (using methods similar to those for adhering antigens for an ELISA assay) and the ability of labelled NK-1 receptor fragment- or receptor analog- expressing cells (e.g., labelled with ³ H-thymidine) to bind the immobilized substance P is used to detect an interaction between substance P and the receptor component. Specific receptor analogues of interest include full-length or partial receptor proteins including an amino acid sequence which differs only by conservative amino acid substitutions, for example, substitution of one amino acid for another of the same class (e.g., valine for glycine, arginine for lysine, etc.) or by one or more non-conservative amino acid substitutions, deletions, or insertions located at positions of the amino acid sequence which do not destroy the receptors' ability to interact with substance P (as assayed above) . Specific receptor fragments of interest include the portions of the receptor deduced to be extracellular. Such regions may be identified by comparison with related proteins of similar structure (e.g., other members of the rhodopsin superfamily) ; useful regions are those exhibiting homology to the extracellular domains of well-

characterized members of the family. Examples of these regions include, without limitation:

(a) MDNVLPVDSDLSPNISTNTSEPNQFVQPAWQ (SEQ ID N0.:4; amino acid residues 1 to 31 of Fig. 1; SEQ ID NO.:3); (b) FTYAVHNEWYYG (SEQ ID NO.:5; amino acid residues 90 to 101 of Fig. 1; SEQ ID NO.:3);

(c) TTETMPSRWCMIEWPEHPNKIYEKVYH (SEQ ID NO.:6; amino acid residues 170 to 197 of Fig. 1; SEQ ID NO.:3); and (d) PYINPDLYLKKFIQQVY (SEQ ID NO.:7; amino acid residues 271 to 287 of Fig. 1; SEQ ID N0.:3).

Alternatively, from the primary amino acid sequence, the secondary protein structure and, therefore, the extracellular domain regions may be deduced semi- empirically using a hydrophobicity/hydrophilicity calculation such as the Chou-Fasman method (see, e.g., Chou and Fasman, Ann . Rev. Biochem . 12:251, 1978). Hydrophilic domains, particularly ones surrounded by hydrophobic stretches (e.g., transmembrane domains) present themselves as strong candidates for extracellular domains. Finally, extracellular domains may be identified experimentally using standard enzymatic digest analysis, e.g., tryptic digest analysis.

Candidate fragments would be tested for interaction with substance P by the assays described herein (e.g., the assay described above). POLYPEPTIDE EXPRESSION

Polypeptides according to the invention may be produced by transformation of a suitable host cell with all or part of an NK-1-encoding cDNA or genomic DNA fragment (e.g., described above) in a suitable expression vehicle, and expression of the receptor. *

Those skilled in the field will understand that any of a wide variety of expression systems may be used to provide the recombinant receptor protein. The precise

host cell used is not critical to the invention, however the following host cells are preferred: Chinese Hamster Ovary (CHO) cells, Madin-Darby Canine Kidney (MDCK) cells, COS cells, and fibroblast cells, such as mouse 3T3 cells. Such cells are available from a wide range of sources (e.g., the American Type Culture Collection, Rockland, MD; Accession Nos. CCL 61, CCL 34, CRL 1650, and CCL 163, respectively) . The method of transfection and the choice of expression vehicle will depend on the host system selected. Mammalian cell transfection methods are described, e.g., in Ausubel et al. (Current Protocols in Molecular Biology, John Wiley & Sons, New York, 1989) ; expression vehicles may be chosen from those provided, e.g., in Cloning Vectors : A Laboratory Manual (P.H. Pouwels et al., 1985, Supp. 1987).

One particularly preferred expression system is the mouse 3T3 fibroblast host cell transfected with a pMAMneo expression vector (Clontech, Palo Alto, CA) . pMAMneo provides: an RSV-LTR enhancer linked to a dexamethasone-inducible MMTV-LTR promoter, an SV40 origin of replication (which allows replication in mammalian systems) , a selectable neomycin gene, and SV40 splicing and polyadenylation sites. DNA encoding the NK-1 receptor or an appropriate receptor fragment or analog (as described above) would be inserted into the pMANneo vector in an orientation designed to allow expression. The recombinant receptor protein would be isolated as described below. Other preferable host cells which may be used in conjunction with the pMAMneo expression vehicle include COS cells and CHO cells (ATCC Accession Nos. CRL 1650 and CCL 61, respectively) .

In another preferred expression system, the NK-l receptor protein (or receptor fragment or analog) is produced by a stably-transfected mammalian cell line. A number of vectors suitable for stable transfection of

mammalian cells are available to the public, e.g., see Pouwels et al. (supra) : methods for constructing such cell lines are also publically available, e.g., in Ausubel et al. (supra) . In one example, cDNA encoding the receptor (or receptor fragment or analog) is cloned into an expression vector which includes the dihydrofolate reductase (DHFR) gene. Integration of the plasmid and, therefore, the NK-1 receptor-encoding gene into the host cell chromosome is selected for by inclusion of 0.01-300 μM methotrexate in the cell culture medium (as described in Ausubel et al., supra) . This dominant selection can be accomplished in most cell types. Recombinant protein expression can be increased by DHFR- ediated amplification of the transfected gene. Methods for selecting cell lines bearing gene amplifications are described in Ausubel et al. (supra) ; such methods generally involve extended culture in medium containing gradually increasing levels of methotrexate. DHFR-containing expression vectors commonly used for this purpose include pCVSEII-DHFR and pAdD26SV(A) (described in Ausubel et al., supra) . Any of the host cells described above or, preferably, a DHFR-deficient CHO cell line (e.g., CHO DHFR~cells, ATCC Accession No. CRL 9096) are among the host cells preferred for DHFR selection of a stably-transfected cell line or DHFR-mediated gene amplification.

Yeast cells may also be used as a host system. Yeast vectors into which the NK-1 receptor- or receptor fragment- or analog-encoding DNA may be cloned are publically available, and many are described in Pouwels et al. (supra) . Methods of yeast transformation are described in Ausubel et al. (supra) .

Once the recombinant NK-1 receptor protein (or fragment or analog, thereof) is expressed, it is isolated, e.g. , using affinity chromatography. In one

example, substance P or anti-NK-1 receptor antibodies (described below) may be attached to a column and used to isolate intact receptor or receptor fragments or analogues. Lysis and fractionation of receptor-harboring cells prior to affinity chromatography may be performed by standard methods (see, e.g., Ausubel et al., supra) . Once isolated, the recombinant protein can, if desired, be further purified, e.g., by high performance liquid chromatography. Receptors of the invention, particularly short receptor fragments, can also be produced by chemical synthesis (e.g., by the methods described in Solid Phase Peptide Synthesis , 2nd ed., 1984, The Pierce Chemical Co., Rockford, IL) . SCREENING FOR NK-1 RECEPTOR ANTAGONISTS

As discussed above, one aspect of the invention features screening for compounds that antagonize the interaction between substance P and the NK-1 receptor, thereby preventing or reducing the cascade of events that are mediated by that interaction. The elements of the screen are the substance P neurotransmitter (or a suitable receptor-binding fragment or analog thereof) and recombinant NK-1 receptor (or a suitable receptor fragment or analog, as outlined above) configured to permit detection of binding.

Substance P may be obtained from the Sigma Chemical Co. (St. Louis, MO) . Alternatively, it may be produced by standard methods of chemical synthesis or recombinant DNA technology given its known amino acid sequence: Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met- NH ₂ (SEQ ID NO. :2) .

Preferably, the NK-1 receptor component is produced by a cell that naturally presents substantially no receptor, e.g., by engineering such a cell to contain nucleic acid encoding the receptor component in an

appropriate expression system. Suitable cells are, e.g., those discussed above with respect to the production of recombinant receptor, most preferably mouse 3T3 fibroblast cells. The assay is preferably performed by fixing the cell expressing the NK-1 receptor component to a solid substrate (e.g., a test tube or microtiter well) by means well known to those in the art, and presenting labelled substance P or a fragment or analog thereof to the cell in the presence of the candidate antagonist. Binding is assayed by the detection label in association with the receptor component (and, therefore, in association with the solid substrate) . Molecules which specifically interfere with labelled substance P binding are considered to be useful in the invention.

The assay format may be any of a number of suitable formats for detecting specific binding, such as a radioimmunoassay format. Preferably, cells transiently or stably transfected with an NK-1 receptor expression vector are immobilized on a microtiter plate and reacted with substance P (or an active fragment or analog thereof) which is detectably labelled, e.g., with a radiolabel or an enzyme which can be assayed, e.g., alkaline phosphatase or horseradish peroxidase. Alternatively, substance P may be adhered to the microtiter plate (using methods similar to those for adhering antigens for an ELISA assay) and the ability of labelled NK-1 receptor expressing cells (e.g., labelled with ³ H-thymidine) can be used to detect specific receptor binding to the immobilized substance P.

In one particular example, a vector expressing the NK-1 receptor (or receptor fragment or analog) is transfected into COS-7 cells (ATCC Accession No. CRL 1651) by the DEAE dextran-chloroquine method (Ausubel et al., supra) . Expression of the receptor protein confers

binding of the substance P neurotransmitter to the cells; substance P does not bind to untransfected host cells or cells bearing the parent vector alone. 10 cm. tissue culture dishes are seeded with freshly trypsinized NK-l receptor-expressing COS-7 cells (750,000 cells, dish) 12- 18h post-transfection. Forty-eight hours later, triplicate dishes are incubated with 2nM radioiodinated substance P (-300 cp /fmol) . This is used as a "control" against which antagonist assays are measured. Such antagonist assays involve incubation of the NK-1 receptor-expressing cells with an equivalent amount of labelled substance P in combination with an appropriate amount of candidate antagonist. An antagonist useful in the invention blocks labelled substance P binding to the immobilized receptor-expressing cells.

Appropriate candidate antagonists include NK-1 receptor fragments, particularly fragments containing an extracellular domain (described above) ; such fragments would preferably including five or more amino acids. Other candidate antagonists include non-peptide compounds designed or derived from analysis of the receptor, as well as anti-NK-1 receptor antibodies. ANTI-NK-1 RECEPTOR ANTIBODIES

NK-1 receptor or receptor fragments or analogues (described above) may be used to raise antibodies by any of the conventional methods well known to those skilled in the art. For example, the cDNA sequence of the NK-1 receptor can be used to select short peptide sequences which can be synthesized (e.g., by chemical synthesis or recombinant DNA techniques) and used to immunize animals, e.g., rabbits, in order to generate antibodies. The antibodies may be polyclonal or monoclonal. Polyclonal antibodies may be enriched in anti-receptor activity, e.g., by column purification (i.e., by using receptor or receptor fragments or analogues immobilized on a column

to screen out the desired antibody, see, e.g., Ausubel et al., supra) . Desired monoclonal antibody-producing hybridomas may also be selected by stimulating and then screening with receptor or receptor fragments or analogues, using standard immunological techniques (see, e.g., Ausubel et al., supra) . THERAPEUTICS AND VACCINES

Particularly suitable therapeutics are the antagonistic receptor fragments (described above) formulated in an appropriate buffer such as physiological saline. Where it is particularly desirable to mimic the receptor conformation at the membrane interface, the fragment may include transmembrane residues adjacent to the extracellular domain of the receptor. In this case, the fragment may be associated with an appropriate lipid fraction (e.g., in lipid vesicles or attached to fragments obtained by disrupting a cell membrane) . Alternatively, anti-NK-1 receptor antibodies produced as described above may be used as a therapeutic. Again, the antibodies would be administered in a pharmaceutically- acceptable buffer (e.g., physiological saline). If appropriate, the antibody preparation may be combined with a suitable adjuvant.

The therapeutic preparation is administered in accordance with the condition to be treated. Ordinarily, it will be administered intravenously, at a dosage that provides suitable competition for substance P binding. Alternatively, it may be convenient to administer the therapeutic orally, nasally, or topically, e.g., as a liquid or a spray, respectively. Again, the dosage would be adjusted to provide suitable competition for NK-1 binding. Treatment may be repeated as necessary for alleviation of pain or disease symptoms.

Because the NK-1 receptor is involved in the transmission of stimuli from the peripheral nervous

system, the therapeutic peptides may be useful for alleviation of pain and may be particularly useful when administered to reduce chronic pain. This association with the nervous system also suggests that the NK-l receptor may facilitate the development of a therapeutic to treat mental-health disorders, such as schizophrenia, or reduce the symptoms of anxiety or stress. Further, the NK-1 receptor's involvement in mononuclear and polymorphonuclear leukocyte activation suggests that an NK-1 receptor antagonist can be used to treat leukocyte- proliferative diseases, e.g., inflammatory diseases, such as arthritis and asthma.

The antibodies of the invention, in a suitable buffer and, if appropriate, including an adjuvant, may also be used as a protective vaccine. Such a vaccine would be administered in a dosage that provides suitable competition for substance P binding over the long-term. Such a vaccine would be useful, e.g., to individuals suffering from chronic pain or any of the disorders (e.g., inflammatory diseases, mental-health disorders) described above.

Other embodiments are within the following claims.