Title:
CORYNEBACTERIUM GLUTAMICUM PROMOTERS
Document Type and Number:
WIPO Patent Application WO2002040679
Kind Code:
A3
Abstract:
The invention relates to isolated polynucleotides from Corynebacterium glutamicum which are useful in the regulation of geneexpression. In particular, the invention relates to isolated polynucleotides comprising C.glutamicum promoters which may be used to regulate, i.e., either increase or decrease, gene expression. In certain embodiments, isolated promoter sequences of the present invention regulate gene expression through the use of exogenous or endogenous induction. The invention further provides recombinant vectors and recombinant cells comprising isolated polynucleotides of the present invention, preferably in operable association with heterologous genes. Also provided are methods of regulating bacterial gene expression comprising growth of a recombinant cell of the present invention. In particular, the present invention provides methods to regulate genes involved in amino acid production comprising growth of a recombinant cell of the present invention. In certain imbodiments, the present invention provides methods of regulating gene expression in bacteria, particularly Corynebacterium species, especially of the genus Corynebacterium, comprising fermentation growth of a recombinant cell of the present invention, where metabolite concentrations, temperature, or oxygen levels are manipulated to regulate gene expression.
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Inventors:
RAYAPATI P JOHN
CRAFTON COREY M
CRAFTON COREY M
Application Number:
PCT/US2001/043096
Publication Date:
May 08, 2003
Filing Date:
November 15, 2001
Export Citation:
Assignee:
ARCHER DANIELS MIDLAND CO (US)
International Classes:
C07K14/34; C12N1/21; C12N15/31; C12N15/77; (IPC1-7): C12N15/31; C12N15/62; C12N15/77; C12N1/21; C12P13/04
Domestic Patent References:
| WO2001000844A2 | 2001-01-04 | |||
| WO1996015246A1 | 1996-05-23 | |||
| WO1988009819A2 | 1988-12-15 |
Foreign References:
| EP1108790A2 | 2001-06-20 |
Other References:
PATEK MIROSLAV ET AL: "Promoters from Corynebacterium glutamicum: Cloning, molecular analysis and search for a consensus motif.", MICROBIOLOGY (READING), vol. 142, no. 5, 1996, pages 1297 - 1309, XP008006983, ISSN: 1350-0872
VASICOVA P ET AL: "Integrative and autonomously replicating vectors for analysis of promoters in Corynebacterium glutamicum.", BIOTECHNOLOGY TECHNIQUES, vol. 12, no. 10, October 1998 (1998-10-01), pages 743 - 746, XP008006984, ISSN: 0951-208X
VASICOVA PAVLA ET AL: "Analysis of the Corynebacterium glutamicum dapA promoter.", JOURNAL OF BACTERIOLOGY, vol. 181, no. 19, October 1999 (1999-10-01), pages 6188 - 6191, XP002211212, ISSN: 0021-9193
REINSCHEID DIETER J ET AL: "Cloning, sequence analysis, expression and inactivation of the Corynebacterium glutamicum pta-ack operon encoding phosphotransacetylase and acetate kinase.", MICROBIOLOGY (READING), vol. 145, no. 2, February 1999 (1999-02-01), pages 503 - 513, XP002211213, ISSN: 1350-0872
VASICOVA P ET AL: "Integrative and autonomously replicating vectors for analysis of promoters in Corynebacterium glutamicum.", BIOTECHNOLOGY TECHNIQUES, vol. 12, no. 10, October 1998 (1998-10-01), pages 743 - 746, XP008006984, ISSN: 0951-208X
VASICOVA PAVLA ET AL: "Analysis of the Corynebacterium glutamicum dapA promoter.", JOURNAL OF BACTERIOLOGY, vol. 181, no. 19, October 1999 (1999-10-01), pages 6188 - 6191, XP002211212, ISSN: 0021-9193
REINSCHEID DIETER J ET AL: "Cloning, sequence analysis, expression and inactivation of the Corynebacterium glutamicum pta-ack operon encoding phosphotransacetylase and acetate kinase.", MICROBIOLOGY (READING), vol. 145, no. 2, February 1999 (1999-02-01), pages 503 - 513, XP002211213, ISSN: 1350-0872
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