Title:
CPA PRIMER, KIT AND DETECTION METHOD FOR KILLING LEUKOTOXIN POSITIVE STAPHYLOCOCCUS AUREUS
Document Type and Number:
WIPO Patent Application WO/2023/000514
Kind Code:
A1
Abstract:
Disclosed in the present invention are a CPA primer, a detection kit and a detection method for killing leukotoxin positive staphylococcus aureus. The CPA primer designed for a target spot pvl comprises stripping primers 4s and 5a, a cross amplification primer 2a1s, and specific primers 2a and 3a, and the nucleotide sequences of the primers are respectively as shown in SEQ ID NO.1 to SEQ ID NO.5. According to the present invention, a pair of stripping primers, cross primers and specific primers are designed for a specific target sequence pvl of staphylococcus aureus toxin-killing toxin, a cross primer isothermal amplification reaction system is constructed, and the detection of the pvl staphylococcus aureus carrying the leukocyte killing toxin gene is realized.
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Inventors:
XU ZHENBO (CN)
LIU ZIQI (CN)
LUO YUTING (CN)
LIU JUNYAN (CN)
CHEN LING (CN)
YE YANRUI (CN)
LI XIAOXI (CN)
HONG WEI (CN)
PENG FANG (CN)
FU XIN (CN)
HU SHUAIFENG (CN)
SU JIANYU (CN)
LIU ZIQI (CN)
LUO YUTING (CN)
LIU JUNYAN (CN)
CHEN LING (CN)
YE YANRUI (CN)
LI XIAOXI (CN)
HONG WEI (CN)
PENG FANG (CN)
FU XIN (CN)
HU SHUAIFENG (CN)
SU JIANYU (CN)
Application Number:
PCT/CN2021/123979
Publication Date:
January 26, 2023
Filing Date:
October 15, 2021
Export Citation:
Assignee:
UNIV SOUTH CHINA TECH (CN)
International Classes:
C12Q1/689; C12N15/11; C12Q1/14; C12Q1/6844
Domestic Patent References:
| WO2021003878A1 | 2021-01-14 |
Foreign References:
| CN109735636A | 2019-05-10 |
Other References:
JIANG HUA, WANG KAN, YAN MUXIA, YE QIAN, LIN XIAOJING, CHEN LING, YE YANRUI, ZHANG LI, LIU JUNYAN, HUANG TENGYI: "Pathogenic and Virulence Factor Detection on Viable but Non-culturable Methicillin-Resistant Staphylococcus aureus", FRONTIERS IN MICROBIOLOGY, vol. 12, XP093025932, DOI: 10.3389/fmicb.2021.630053
"Thesis for Academic Master Degree Jiangsu University", 15 February 2021, JIANGSU UNIVERSITY, CN, article ZHOU ZHIQING: "Establishment of Rapid Detection Technology for Mrsa Bacteria with pvl and Study on Its Pathogenic Mechanism)", pages: 1 - 75, XP093025933
FUNAKI TOSHITAKA, YASUHARA TSUTOMU, KUGAWA SATOSHI, YAMAZAKI YOHEI, SUGANO EMI, NAGAKURA YOSHIMI, YOSHIDA KATSUHIKO, FUKUCHI KUNIH: "SCCmec typing of PVL-positive community-acquired Staphylococcus aureus (CA-MRSA) at a Japanese hospital", HELIYON, ELSEVIER LTD, GB, vol. 5, no. 3, 1 March 2019 (2019-03-01), GB , pages e01415, XP093025936, ISSN: 2405-8440, DOI: 10.1016/j.heliyon.2019.e01415
"Thesis for Academic Master Degree Jiangsu University", 15 February 2021, JIANGSU UNIVERSITY, CN, article ZHOU ZHIQING: "Establishment of Rapid Detection Technology for Mrsa Bacteria with pvl and Study on Its Pathogenic Mechanism)", pages: 1 - 75, XP093025933
FUNAKI TOSHITAKA, YASUHARA TSUTOMU, KUGAWA SATOSHI, YAMAZAKI YOHEI, SUGANO EMI, NAGAKURA YOSHIMI, YOSHIDA KATSUHIKO, FUKUCHI KUNIH: "SCCmec typing of PVL-positive community-acquired Staphylococcus aureus (CA-MRSA) at a Japanese hospital", HELIYON, ELSEVIER LTD, GB, vol. 5, no. 3, 1 March 2019 (2019-03-01), GB , pages e01415, XP093025936, ISSN: 2405-8440, DOI: 10.1016/j.heliyon.2019.e01415
Attorney, Agent or Firm:
GUANGZHOU HUAXUE INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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