Title:
CRISPR-BASED NUCLEIC ACID DETECTION KIT AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/207909
Kind Code:
A1
Abstract:
Disclosed is a CRISPR-based detection kit and use thereof. The detection kit of the present invention comprises a gene editing system. The gene editing system comprises a nuclease and a guide RNA. The sequence of the guide RNA is selected from one or more of nucleotide sequences set forth in SEQ ID NOs: 1-4. The kit of the present invention can reduce the detection limit while improving the detection efficiency within a shortened reaction time and reduced reaction temperature. The operation does not include nucleic acid extraction, eliminating the need for uncovering, thus being simple, convenient, and rapid.
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Inventors:
LIU JIA (CN)
QU JIEMING (CN)
QU JIEMING (CN)
Application Number:
PCT/CN2023/090320
Publication Date:
November 02, 2023
Filing Date:
April 24, 2023
Export Citation:
Assignee:
UNIV SHANGHAI TECHNOLOGY (CN)
RUIJIN HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE (CN)
RUIJIN HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE (CN)
International Classes:
C12N15/113; C12N9/22; C12N15/11; C12Q1/68; C12Q1/6806; C12Q1/6844; C12Q1/70; C12R1/93
Domestic Patent References:
| WO2021240443A1 | 2021-12-02 |
Foreign References:
| CN115029345A | 2022-09-09 | |||
| CN111534643A | 2020-08-14 | |||
| CN113528686A | 2021-10-22 | |||
| CN112176107A | 2021-01-05 |
Other References:
AIXIA ZHANG, ZHU QINGFENG;CHEN PEI;YU YANG;WEI WENKANG: "RNA Editing System Based on CRISPR/Cas13 and Its Application in Nucleic Acid Detection", GUANGDONG AGRICULTURAL SCIENCES, vol. 47, no. 11, 28 October 2020 (2020-10-28), pages 243 - 251, XP093103794, ISSN: 1004-874X, DOI: 10.16768/j.issn.1004-874X.2020.11.027
SAFARI, F. ET AL.: "CRISPR Systems: Novel Approaches for Detection and Combating COVID-19", VIRUS RESEARCH, vol. 294, 8 January 2021 (2021-01-08), XP086472850, DOI: 10.1016/j.virusres.2020.198282
SAFARI, F. ET AL.: "CRISPR Systems: Novel Approaches for Detection and Combating COVID-19", VIRUS RESEARCH, vol. 294, 8 January 2021 (2021-01-08), XP086472850, DOI: 10.1016/j.virusres.2020.198282
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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