Title:
CULTURE METHOD, CULTURE PRODUCT, SPHEROID, AND METHOD FOR SCREENING FOR TEST SUBSTANCE
Document Type and Number:
WIPO Patent Application WO/2023/080145
Kind Code:
A1
Abstract:
This culture method includes culturing a spheroid of human neural cell-like cells in the presence of a tau protein aggregate and then culturing the culture product in a culture medium containing a lipid in an amount of 5 μg/mL or more, in which the lipid comprises at least one component selected from the group consisting of a glycerolipid, a glycerophospholipid and a sphingolipid. The culture method includes culturing a spheroid of human neural cell-like cells in the presence of a tau protein aggregate and then culturing the culture product in a culture medium containing a neurotrophic factor.
Inventors:
HIRAMINE HAYATO (JP)
NAGASHIMA RYOSUKE (JP)
MAEDA SUMIHIRO (JP)
OKANO HIDEYUKI (JP)
ISHIKAWA MITSURU (JP)
NAGASHIMA RYOSUKE (JP)
MAEDA SUMIHIRO (JP)
OKANO HIDEYUKI (JP)
ISHIKAWA MITSURU (JP)
Application Number:
PCT/JP2022/040950
Publication Date:
May 11, 2023
Filing Date:
November 02, 2022
Export Citation:
Assignee:
JSR CORP (JP)
UNIV KEIO (JP)
UNIV KEIO (JP)
International Classes:
C12N5/079; C12Q1/02; C12N15/12
Domestic Patent References:
| WO2016076435A1 | 2016-05-19 | |||
| WO2021090877A1 | 2021-05-14 |
Attorney, Agent or Firm:
MATSUNUMA Yasushi et al. (JP)
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