Title:
CYANOHYDRIN ETHERS AND ESTERS AS HIGH-SENSITIVITY ENZYME SUBSTRATES
Document Type and Number:
WIPO Patent Application WO2003104167
Kind Code:
A3
Abstract:
Ethers and esters of cyanohydrins that contain optically detectable moieties are highly effective for detecting, monitoring, and measuring the activity of enzymes that cause the cleavage of certain types of substrates. The cyanohydrins function as proaldehydes and proketones, spontaneously converting to aldehydes and ketones, respectively, which provide a large increase in optical detectability relative to both the starting esters and ethers and the cyanohydrins.
Inventors:
HAMMOCK BRUCE D
SHAN GUOMIN
ZHANG RONG
SHAN GUOMIN
ZHANG RONG
Application Number:
PCT/US2002/031623
Publication Date:
March 25, 2004
Filing Date:
October 02, 2002
Export Citation:
Assignee:
UNIV CALIFORNIA (US)
International Classes:
C07C255/37; C07C255/38; C07C255/39; C07C271/24; C07D295/155; C07D303/40; C07K5/087; (IPC1-7): C12N9/10; C07C255/03; C07C255/30
Other References:
SHAN G. ET AL.: "Development of sensitive esterase assays based on alpha-cyano-containing esters", ANALYTICAL BIOCHEMISTRY, vol. 299, 2001, pages 54 - 62, XP002972463
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