DECARBOXYLASE PROTEINS WITH HIGH KETO-ISOVALERATE DECARBOXYLASE ACTIVITY

Title:

DECARBOXYLASE PROTEINS WITH HIGH KETO-ISOVALERATE DECARBOXYLASE ACTIVITY

Document Type and Number:

WIPO Patent Application WO/2013/016724

Kind Code:

A3

Abstract:

The present invention relates to recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise at least one nucleic acid molecule encoding a polypeptide with keto-isovalerate decarboxylase (KIVD) activity, wherein said polypeptide is at least about 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to a polypeptide selected from SEQ ID NOs: 1-214. Also provided are modified decarboxylases exhibiting an improved ability to utilize α-ketoisovalerate as a substrate in various beneficial enzymatic conversions.

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Inventors:

DUNDON CATHERINE ASLESON (US)
ROBERG-PEREZ KEVIN (US)
SNOW CHRISTOPHER (US)
MEINHOLD PETER (US)

Application Number:

PCT/US2012/048802

Publication Date:

June 06, 2013

Filing Date:

July 30, 2012

Export Citation:

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Assignee:

GEVO INC (US)
DUNDON CATHERINE ASLESON (US)
ROBERG-PEREZ KEVIN (US)
SNOW CHRISTOPHER (US)
MEINHOLD PETER (US)

International Classes:

C12N15/00

Domestic Patent References:

WO2010051527A2

2010-05-06

Foreign References:

US20080274526A1	2008-11-06
US7851188B2	2010-12-14

Other References:

DATABASE GENBANK 20 May 2011 (2011-05-20), GAO Y. ET AL.: "Lactococcus lactis subsp. lactis CV56, complete genome", retrieved from urf: http:/lwww.ncbi.nlm.nih.gov/nuccore/326405597?sat=148satkey=10498990 accession no. P002365
ZHANG K. ET AL.: "Expanding metabolism for biosynthesis of nonnatural alcohols", PROC. NATL. ACAD. SCI. USA, vol. 105, no. 52, 30 December 2008 (2008-12-30), pages 20653 - 20658, XP002576547

Attorney, Agent or Firm:

BLINKA, Thomas A. et al. (777 6th Street N.W.,Suite 110, Washington District of Columbia, US)

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