Title:
DETECTION OF LIGAND/ANTILIGAND COMPLEX FORMATION BY ELECTROMAGNETICALLY DETECTABLE BULK PROPERTY MEASUREMENT
Document Type and Number:
WIPO Patent Application WO2004008105
Kind Code:
A3
Abstract:
Disclosed are methods for analyzing molecular binding events in which the formation of ligand/antiligand complexes can be directly detected in a mixture without requiring separation of the components of the mixture from each other by measuring bulk properties of the mixture (i.e., properties that have contributions from several or all of the components present in the mixture). Using these techniques, it is possible to screen libraries without labeling either the target antiligand or ligand. The invention also provides a method for determining the strength of ligand/antiligand binding by further analysis of the same signals.
Inventors:
CHAPMAN ROBERT G (US)
GHANOUNI PEJMAN (US)
SHMULEVICH IGOR A (US)
BALABAN DAVID (US)
HEANUE JOSEPH (US)
MAY ANDREW P (US)
SHARKOV NIKOLAI A (US)
GHANOUNI PEJMAN (US)
SHMULEVICH IGOR A (US)
BALABAN DAVID (US)
HEANUE JOSEPH (US)
MAY ANDREW P (US)
SHARKOV NIKOLAI A (US)
Application Number:
PCT/US2003/022526
Publication Date:
October 14, 2004
Filing Date:
July 17, 2003
Export Citation:
Assignee:
MDS INC DBA MDS SCIEX (CA)
CHAPMAN ROBERT G (US)
GHANOUNI PEJMAN (US)
SHMULEVICH IGOR A (US)
BALABAN DAVID (US)
HEANUE JOSEPH (US)
MAY ANDREW P (US)
SHARKOV NIKOLAI A (US)
CHAPMAN ROBERT G (US)
GHANOUNI PEJMAN (US)
SHMULEVICH IGOR A (US)
BALABAN DAVID (US)
HEANUE JOSEPH (US)
MAY ANDREW P (US)
SHARKOV NIKOLAI A (US)
International Classes:
C12Q1/00; C12Q1/68; G01N3/00; G01N21/00; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/551; G01N33/567; G06F19/00; G01N; (IPC1-7): C12Q1/68; C12Q1/00; G01N3/00; G01N21/00; G01N33/00; G01N33/48; G01N33/53; G01N33/543; G01N33/551; G01N33/567
Other References:
MALMQVIST ET AL: "Biomolecular interaction analysis: affinity biosensor technologies for functional analysis of proteins", CURRENT OPINION IN CHEMICAL BIOLOGY, vol. 1, 1997, pages 378 - 383, XP002978720
LIEB SG: "Simplex Method of Nonlinear Least-Squares - A Logical Complementary Method to Linear Least-Square Analysis of Data", J. OF CHEMICAL EDUCATION, vol. 74, no. 8, August 1997 (1997-08-01), pages 1008 - 1011, XP002978777
LIEB SG: "Simplex Method of Nonlinear Least-Squares - A Logical Complementary Method to Linear Least-Square Analysis of Data", J. OF CHEMICAL EDUCATION, vol. 74, no. 8, August 1997 (1997-08-01), pages 1008 - 1011, XP002978777
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