Title:
DETECTION METHOD AND DETECTION KIT FOR HIGH-SENSITIVITY SARS-COV-2 NEUTRALIZING ANTIBODY
Document Type and Number:
WIPO Patent Application WO/2022/206401
Kind Code:
A1
Abstract:
Disclosed in the present invention are a detection method and a detection kit for a high-sensitivity SARS-CoV-2 neutralizing antibody, belonging to the technical field of biomedical detection. The kit of the present invention comprises a chromatographic test paper, a card shell and a sample diluent, wherein the chromatographic test paper comprises a bottom plate, a sample pad, a binding pad, an NC membrane and a water-absorbing pad, the NC membrane is sequentially provided with a capture line, a detection line and a quality control line, the capture line is coated with an ACE2 protein, the detection line is coated with an RBD protein, and the binding pad is provided with an RBD protein marker. According to the present invention, the sensitivity of detecting the neutralizing antibody is improved by means of using the principle of a blocking method and a sandwich method; and a non-neutralizing antibody targeting the RBD is captured in advance by means of adding the capture line, so that the specificity of subsequently detecting the neutralizing antibody by means of the sandwich method is ensured.
Inventors:
ZHANG YUJI (CN)
Application Number:
PCT/CN2022/081424
Publication Date:
October 06, 2022
Filing Date:
March 17, 2022
Export Citation:
Assignee:
NANJING LEADING MEDICAL TECH CO LTD (CN)
International Classes:
G01N33/68; G01N33/543; G01N33/558; G01N33/569
Foreign References:
| CN112730851A | 2021-04-30 | |||
| CN211905393U | 2020-11-10 | |||
| CN111879933A | 2020-11-03 | |||
| CN112485436A | 2021-03-12 | |||
| JP2009002822A | 2009-01-08 |
Attorney, Agent or Firm:
ZHONG LUN LAW FIRM (CN)
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