Title:
DETECTION METHOD
Document Type and Number:
WIPO Patent Application WO/2020/233503
Kind Code:
A1
Abstract:
Provided is a method for assessing cytotoxicity of a cytotoxic cell that includes culturing a plurality of the cytotoxic cells with a plurality of target cells in a microfluidic device and determining whether the cytotoxic cells have a cytotoxic effect on the target cells, such as by determining a cell count and/or status of the cytotoxic cells and/or the target cells. A kit and a system for use in the aforementioned method are also provided.
Inventors:
WONG HANG HENG (CN)
WANG HAITAO (CN)
WONG KUAN UN (CN)
WANG HAITAO (CN)
WONG KUAN UN (CN)
Application Number:
PCT/CN2020/090425
Publication Date:
November 26, 2020
Filing Date:
May 15, 2020
Export Citation:
Assignee:
UNIV MACAU (CN)
AW MEDICAL CO LTD (CN)
AW MEDICAL CO LTD (CN)
International Classes:
C07K16/28; C07K16/30; G01N33/569
Domestic Patent References:
| WO2017123978A1 | 2017-07-20 | |||
| WO2017053902A1 | 2017-03-30 |
Other References:
PRADIP BAJGAIN; SUPANNIKAR TAWINWUNG; LINDSEY D’ELIA; SUJITA SUKUMARAN; NORIHIRO WATANABE; VALENTINA HOYOS; PREMAL LULLA; MALCOLMK: "CAR T cell therapy for breast cancer:harnessing the tumor milieu to drive T cell activation", JOURNAL FOR IMMUNOTHERAPY OF CANCER, vol. 6, no. 1, 10 May 2018 (2018-05-10), pages 1 - 13, XP021256347, DOI: :10.1186/s40425-018-0347-5
ADA HANG-HENG WONG, HAORAN LI, YANWEI JIA, PUI-IN MAK, RUI PAULO DA SILVA MARTINS, YAN LIU, CHI MAN VONG, HANG CHEONG WONG, PAK KI: "Drug screening of cancer cell lines and human primary tumors using droplet microfluidics", SCIENTIFIC REPORTS, vol. 7, no. 1, 9109, 22 August 2017 (2017-08-22), pages 1410 - 1412, XP055755749, DOI: 10.1038/s41598-017-08831-z
ANDREA K. POMERANTZ, SARI-SARRAF FARID, GROVE KERRI J., PEDRO LILIANA, RUDEWICZ PATRICK J., FATHMAN JOHN W., KRUCKER THOMAS: "Enabling drug discovery and development through single-cell imaging", EXPERT OPINION ON DRUG DISCOVERY, vol. 14, no. 2, 24 December 2018 (2018-12-24), pages 115 - 125, XP055756587, ISSN: 1746-0441, DOI: 10.1080/17460441.2019.1559147
SHI,X. M. ET AL: "Progress in detection methods of cytotoxic T lymphocyte activity", ZHONGGUO MIANYIXUE ZAZHI = CHINESE JOURNAL OF IMMUNOLOGY, vol. 31, no. 3, 31 December 2015 (2015-12-31), pages 439 - 432, XP009524432, ISSN: 1000-484X, DOI: 10.3969/j.issn.1000-484X.2015.03.033
ADA HANG-HENG WONG, HAORAN LI, YANWEI JIA, PUI-IN MAK, RUI PAULO DA SILVA MARTINS, YAN LIU, CHI MAN VONG, HANG CHEONG WONG, PAK KI: "Drug screening of cancer cell lines and human primary tumors using droplet microfluidics", SCIENTIFIC REPORTS, vol. 7, no. 1, 9109, 22 August 2017 (2017-08-22), pages 1410 - 1412, XP055755749, DOI: 10.1038/s41598-017-08831-z
ANDREA K. POMERANTZ, SARI-SARRAF FARID, GROVE KERRI J., PEDRO LILIANA, RUDEWICZ PATRICK J., FATHMAN JOHN W., KRUCKER THOMAS: "Enabling drug discovery and development through single-cell imaging", EXPERT OPINION ON DRUG DISCOVERY, vol. 14, no. 2, 24 December 2018 (2018-12-24), pages 115 - 125, XP055756587, ISSN: 1746-0441, DOI: 10.1080/17460441.2019.1559147
SHI,X. M. ET AL: "Progress in detection methods of cytotoxic T lymphocyte activity", ZHONGGUO MIANYIXUE ZAZHI = CHINESE JOURNAL OF IMMUNOLOGY, vol. 31, no. 3, 31 December 2015 (2015-12-31), pages 439 - 432, XP009524432, ISSN: 1000-484X, DOI: 10.3969/j.issn.1000-484X.2015.03.033
Attorney, Agent or Firm:
BEIJING PAT SF INTELLECTUAL PROPERTY AGENCY CO. LTD (CN)
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