Title:
DETECTION, QUANTIFICATION, AND EXPRESSION ANALYSIS OF FULL VIRAL CAPSIDS
Document Type and Number:
WIPO Patent Application WO/2023/212616
Kind Code:
A3
Abstract:
Methods and apparatuses that may be useful for detecting, quantifying, and/or analyzing viral capsids may include a viral vector with nucleic acid region with a promoter at the 5' end; a gene of interest under control of the promoter, wherein the gene of interest includes a polyadenylation signal sequence; and a nucleic acid segment encoding a CRISPR guide RNA (gRNA) element, wherein the nucleic acid segment is downstream of the gene of interest, and the gene of interest and the gRNA element are separated by the polyadenylation signal sequence. The CRISPR guide RNA element may control expression of a detectable protein, indicative of viral capsid status.
Inventors:
VITU ELVIRA (US)
GOODWIN NEAL (US)
NEUFELD BELLA (US)
GUNSTREAM STEPHEN (US)
GOODWIN NEAL (US)
NEUFELD BELLA (US)
GUNSTREAM STEPHEN (US)
Application Number:
PCT/US2023/066264
Publication Date:
December 07, 2023
Filing Date:
April 26, 2023
Export Citation:
Assignee:
ALPHA TEKNOVA INC (US)
International Classes:
C12N15/86; A61K48/00; C12N9/22; C12N15/62
Domestic Patent References:
| WO2016094872A1 | 2016-06-16 | |||
| WO2021173359A1 | 2021-09-02 |
Foreign References:
| US20210392862A1 | 2021-12-23 | |||
| US20190264186A1 | 2019-08-29 | |||
| US20180187172A1 | 2018-07-05 |
Attorney, Agent or Firm:
GONT, Linda K. (US)
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