Title:
DETECTION AND QUANTITATIVE METHOD FOR POST-TRANSLATIONAL MODIFICATION PROTEOMICS
Document Type and Number:
WIPO Patent Application WO/2018/170981
Kind Code:
A1
Abstract:
Disclosed is a comparative proteomic technique. In particular, disclosed is a detection and quantitative method for post-translational modification proteomics. The method labels the protein sample to be detected and the internal standard with equiponderous tandem mass tags, and carries out tandem mass spectrometry for the labelled mixture, wherein the internal standard is a peptide fragment mixture rich in post-translational modifications to be detected.
Inventors:
DAI LUNZHI (CN)
Application Number:
PCT/CN2017/081059
Publication Date:
September 27, 2018
Filing Date:
April 19, 2017
Export Citation:
Assignee:
UNIV SICHUAN (CN)
International Classes:
H01J37/32; G01N1/28; G01N33/68
Other References:
THOMPSON, J.W. ET AL.: "Solid-Phase Capture for the Detection and Relative Quantification of S-nitrosoproteins by Mass Spectrometry", METHODS, vol. 62, no. 2, 1 August 2013 (2013-08-01), pages 130 - 137, XP055539929
CHENG, L. ET AL.: "Peptide Labeling Using Isobaric Tagging Reagents for Quantitative Phosphoproteomics", METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J., vol. 1355, 1 January 2016 (2016-01-01), pages 53 - 70
WANG, ZHIJIE ET AL.: "Determination of Chloramphenicol, Thiamphenicol and Florfenicol Residues in Aquatic Products by HPLC-MS with Internal Standard Method", PROGRESS IN FISHERY SCIENCES, vol. 30, no. 2, 30 April 2009 (2009-04-30), pages 115 - 119
CHENG, L. ET AL.: "Peptide Labeling Using Isobaric Tagging Reagents for Quantitative Phosphoproteomics", METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J., vol. 1355, 1 January 2016 (2016-01-01), pages 53 - 70
WANG, ZHIJIE ET AL.: "Determination of Chloramphenicol, Thiamphenicol and Florfenicol Residues in Aquatic Products by HPLC-MS with Internal Standard Method", PROGRESS IN FISHERY SCIENCES, vol. 30, no. 2, 30 April 2009 (2009-04-30), pages 115 - 119
Attorney, Agent or Firm:
CHOFN INTELLECTUAL PROPERTY (CN)
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