Title:
DIGITAL PCR METHOD FOR INTEGRATED MICRO-DROPLET CHIP
Document Type and Number:
WIPO Patent Application WO/2024/098295
Kind Code:
A1
Abstract:
The present invention provides a digital PCR method for an integrated micro-droplet chip, which belongs to the technical field of digital PCR analyzers. The method comprises: controlling a sample adding cavity and a gas-liquid interface to form a first pressure difference therebetween and controlling an oil-liquid interface and the gas-liquid interface to form a second pressure difference therebetween to drive a sample in the sample adding cavity and a generated oil of the oil-liquid interface, respectively, to enter a micro-droplet generation structure, and storing generated micro-droplets in a reaction cavity; placing the reaction cavity in a heating module for heating and amplification; controlling an external pressure to drive a detection pushing oil to enter the reaction cavity from the gas-liquid interface to allow the micro-droplets in the reaction cavity flow from the reaction cavity to the micro-droplet generation structure, and driving an detection separation oil, by external pressure, to enter the micro-droplet generation structure from the oil-liquid interface, so that the detection separation oil separates the micro-droplets flowing from the reaction cavity to the micro-droplet generation structure to form queues, and the micro-droplets enter a fluorescence detection area. According to the present application, time-division multiplexing of the micro-droplet generation structure allows generation, amplification, and detection of droplets to be integrated in one chip, thus realizing a fully integrated and totally enclosed digital PCR process.
Inventors:
SU SHISHENG (CN)
LIU XIAOBIN (CN)
XU BO (CN)
LIU JINWEI (CN)
HAN YINGMIN (CN)
ZHOU HAIYU (CN)
DU XIAOCHUN (CN)
YANG WENJUN (CN)
WANG YONGDOU (CN)
LIU XIAOBIN (CN)
XU BO (CN)
LIU JINWEI (CN)
HAN YINGMIN (CN)
ZHOU HAIYU (CN)
DU XIAOCHUN (CN)
YANG WENJUN (CN)
WANG YONGDOU (CN)
Application Number:
PCT/CN2022/130941
Publication Date:
May 16, 2024
Filing Date:
November 09, 2022
Export Citation:
Assignee:
TARGETINGONE TECH BEIJING CORPORATION (CN)
International Classes:
C12Q1/686; C12M1/00
Foreign References:
| CN112553063A | 2021-03-26 | |||
| CN109752353A | 2019-05-14 | |||
| CN114196741A | 2022-03-18 | |||
| CN216808822U | 2022-06-24 | |||
| CN114085762A | 2022-02-25 | |||
| CN216550456U | 2022-05-17 | |||
| CN115181654A | 2022-10-14 | |||
| CN115094120A | 2022-09-23 | |||
| CN114085751A | 2022-02-25 | |||
| CN109825426A | 2019-05-31 |
Attorney, Agent or Firm:
BEIJING FAIRNESSLS (XUANFA) LAW FIRM (CN)
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