Title:
DIRECT MULTIPLEX CHARACTERIZATION OF GENOMIC DNA
Document Type and Number:
WIPO Patent Application WO2002057491
Kind Code:
A3
Abstract:
The invention is directed to novel methods of multiplexing nucleic acid reactions, including amplification, detection and genotyping. The invention relies on the use of percircle probes that are circularized in the presence of the corresponding target nucleic acids, cleaved, and then amplified.
Inventors:
WILLIS THOMAS D
HARDENBOL PAUL
JAIN MANHEESH
STOLC VIKTOR
RONAGHI MOSTAFA
DAVIS RONALD W
HARDENBOL PAUL
JAIN MANHEESH
STOLC VIKTOR
RONAGHI MOSTAFA
DAVIS RONALD W
Application Number:
PCT/US2001/047672
Publication Date:
October 09, 2003
Filing Date:
October 24, 2001
Export Citation:
Assignee:
UNIV LELAND STANFORD JUNIOR (US)
International Classes:
G01N33/53; C07H21/02; C07H21/04; C12N15/09; C12P19/34; C12Q1/48; C12Q1/68; C12Q1/6827; C12Q1/6858; C12Q1/686; G01N33/566; (IPC1-7): C12Q1/68
Domestic Patent References:
| WO1998004746A1 | 1998-02-05 | |||
| WO1996015271A1 | 1996-05-23 |
Foreign References:
| US5854033A | 1998-12-29 | |||
| US5660988A | 1997-08-26 |
Other References:
SHOEMAKER D D ET AL: "QUANTITATIVE PHENOTYPIC ANALYSIS OF YEAST DELETION MUTANTS USING A HIGHLY PARALLEL MOLECULAR BAR-CODING STRATEGY", NATURE GENETICS, NEW YORK, NY, US, vol. 14, no. 4, 1 December 1996 (1996-12-01), pages 450 - 456, XP002043431, ISSN: 1061-4036
THOMAS D C ET AL: "AMPLIFICATION OF PADLOCK PROBES FOR DNA DIAGNOSTICS BY CASCADE ROLLING CIRCLE AMPLIFICATION OR THE POLYMERASE CHAIN REACTION", ARCHIVES OF PATHOLOGY, AMERICAN MEDICAL ASSOCIATION, CHICAGO, IL,, US, vol. 123, no. 12, December 1999 (1999-12-01), pages 1170 - 1176, XP008001739, ISSN: 0003-9985
THOMAS D C ET AL: "AMPLIFICATION OF PADLOCK PROBES FOR DNA DIAGNOSTICS BY CASCADE ROLLING CIRCLE AMPLIFICATION OR THE POLYMERASE CHAIN REACTION", ARCHIVES OF PATHOLOGY, AMERICAN MEDICAL ASSOCIATION, CHICAGO, IL,, US, vol. 123, no. 12, December 1999 (1999-12-01), pages 1170 - 1176, XP008001739, ISSN: 0003-9985
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