Title:
DISPLAY OF VIRAL PROTEINS AS LIGANDS FOR CELL-SURFACE RECEPTOR
Document Type and Number:
WIPO Patent Application WO1999060148
Kind Code:
A3
Abstract:
The invention relates to the targeted delivery of substances to cells. The invention provides a method for selecting at least one mutant protein derived from a viral protein as a ligand capable of binding to a cell-surface receptor, comprising displaying at least one mutant of said protein on the surface of a micro-organism expressing said protein, further comprising selecting at least one such a mutant micro-organism for its capacity to bind to said receptor further comprising incorporating said protein in a virus-like particle or gene-delivery vehicle allowing targeting of said virus-like particle or gene-delivery vehicle to cells carrying said receptor.
Inventors:
VAN ES HELMUTH
Application Number:
PCT/NL1999/000311
Publication Date:
January 13, 2000
Filing Date:
May 20, 1999
Export Citation:
Assignee:
INTROGENE BV (NL)
International Classes:
A61K47/48; C07K14/15; C12N1/21; C12N7/04; C12N15/10; C12N15/861; C12N15/867; C12N15/87; C40B40/02; A61K48/00; (IPC1-7): C12N15/87; C12N15/86; C12N7/04; A61K47/48; C12N15/10
Domestic Patent References:
| WO1995026412A1 | 1995-10-05 | |||
| WO1993025234A1 | 1993-12-23 |
Foreign References:
| EP0705844A1 | 1996-04-10 | |||
| FR2758821A1 | 1998-07-31 |
Other References:
HONG S S ET AL: "PROTEIN LIGANDS OF THE HUMAN ADENOVIRUS TYPE 2 OUTER CAPSID IDENTIFIED BY BIOPANNING OF A PHAGE-DISPLAYED PEPTIDE LIBRARY ON SEPARATE DOMAINS OF WILD-TYPE AND MUTANT PENTON CAPSOMERS", EMBO JOURNAL, vol. 14, no. 19, 1995, pages 4714 - 4727, XP002051922
ALBRITTON L M ET AL: "A PUTATIVE MURINE ECOTROPIC RETROVIRUS RECEPTOR GENE ENCODES A MULTIPLE MEMBRANE-SPANNING PROTEIN AND CONFERS SUSCEPTIBILITY TO VIRUS INFECTION", CELL, vol. 57, 19 May 1989 (1989-05-19), pages 659 - 666, XP002036212
ALBRITTON, L. M. ET AL.: "Envelope-binding domain in the cationic amino acid transporter determines the host range of ecotropic murine retroviruses", JOURNAL OF VIROLOGY., vol. 67, no. 4, April 1993 (1993-04-01), ICAN SOCIETY FOR MICROBIOLOGY US, pages 2091 - 2096, XP002084804
KRASNYKH V N ET AL: "GENERATION OF RECOMBINANT ADENOVIRUS VECTORS WITH MODIFIED FIBERS FOR ALTERING VIRAL TROPISM", JOURNAL OF VIROLOGY, vol. 70, no. 10, October 1996 (1996-10-01), pages 6839 - 6846, XP002067518
ALBRITTON L M ET AL: "A PUTATIVE MURINE ECOTROPIC RETROVIRUS RECEPTOR GENE ENCODES A MULTIPLE MEMBRANE-SPANNING PROTEIN AND CONFERS SUSCEPTIBILITY TO VIRUS INFECTION", CELL, vol. 57, 19 May 1989 (1989-05-19), pages 659 - 666, XP002036212
ALBRITTON, L. M. ET AL.: "Envelope-binding domain in the cationic amino acid transporter determines the host range of ecotropic murine retroviruses", JOURNAL OF VIROLOGY., vol. 67, no. 4, April 1993 (1993-04-01), ICAN SOCIETY FOR MICROBIOLOGY US, pages 2091 - 2096, XP002084804
KRASNYKH V N ET AL: "GENERATION OF RECOMBINANT ADENOVIRUS VECTORS WITH MODIFIED FIBERS FOR ALTERING VIRAL TROPISM", JOURNAL OF VIROLOGY, vol. 70, no. 10, October 1996 (1996-10-01), pages 6839 - 6846, XP002067518
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