Title:
DNA ARRAYS FOR MEASURING SENSITIVITY TO ANTICANCER AGENT
Document Type and Number:
WIPO Patent Application WO/2003/004640
Kind Code:
A1
Abstract:
DNA arrays for measuring sensitivity to a metabolic antagonist−type anticancer agent or a combined use of such an anticancer agent with another anticancer agent, characterized by having at least 13 types of target gene fragments, involving respectively at least two types of genes selected from among nucleic acid metabolism−associated enzyme genes, gene repair−associated enzyme genes, drug tolerance−associated factor genes and housekeeping genes, wherein these gene fragments have been selected by the following steps 1) and 2) and immobilized on a substrate&semi 1) the step of selecting fragments having high specificity for target genes by searching the homology with the use of data base&semi and 2) the step of performing northern hybridization against RNA obtained from tumor cells with the use of the fragments selected in the step 1) as probes to thereby confirm the specificity for the target genes. Thus, expression of several ten to several hundred genes in a specimen can be conveniently measured by a single assay procedure at a high qualification level.
More Like This:
Inventors:
TAKECHI TEIJI (JP)
KOIZUMI KATSUHISA (JP)
AZUMA ATSUSHI (JP)
FUKUSHIMA MASAKAZU (JP)
KOIZUMI KATSUHISA (JP)
AZUMA ATSUSHI (JP)
FUKUSHIMA MASAKAZU (JP)
Application Number:
PCT/JP2002/006754
Publication Date:
January 16, 2003
Filing Date:
July 03, 2002
Export Citation:
Assignee:
TAIHO PHARMACEUTICAL CO LTD (JP)
TAKECHI TEIJI (JP)
KOIZUMI KATSUHISA (JP)
AZUMA ATSUSHI (JP)
FUKUSHIMA MASAKAZU (JP)
TAKECHI TEIJI (JP)
KOIZUMI KATSUHISA (JP)
AZUMA ATSUSHI (JP)
FUKUSHIMA MASAKAZU (JP)
International Classes:
C12Q1/68; (IPC1-7): C12N15/09; C12Q1/68
Domestic Patent References:
| WO2001032928A2 | 2001-05-10 | |||
| WO2001075160A1 | 2001-10-11 |
Other References:
SCHERF U. ET AL.: "A gene expression database for the molecular pharmacology of cancer", NAT. GENET., vol. 24, no. 3, 2000, pages 236 - 244, XP002225993
TSUNODA T. ET AL.: "Diagnosis system of drug sensitivity of cancer using cDNA microarray and multivariate statistical anaylsis", FRONTIERS SCIENCE SERIES, vol. 30, 2000, pages 16 - 17, XP002960710
GREM J.L. ET AL.: "Thymidine kinase, thymidylate synthase and dihydropyrimidine dehydrogenase profiles of cell lines of the National Cancer Institute's Anticancer Frug Scteen", CLIN. CANCER RES., vol. 7, no. 4, April 2001 (2001-04-01), pages 999 - 1009, XP002960711
ISHIKAWA Y. ET AL.: "Dihydropyrimidine dehydrogenase activity and messenger RNA level may be related to the antitumor effect of 5-fluorouracil on human tumor xenografts in nude mice", CLIN. CANCER RES., vol. 5, no. 4, 1999, pages 883 - 889, XP002960712
SALONGA D. ET AL.: "Colorectal tumors responding to 5-fluorouracil have low gene expression levels of dihydropyrimidine dehydrogenase, thymidylate synthase and thymidine phosphorylase", CLIN. CANCER RES., vol. 6, no. 4, 2000, pages 1322 - 1327, XP002960713
TAKUMI OCHIAI ET AL.: "Daicho-gan ni okeru orotate phosphoribosyl transferase (OPRT), thymidine kinase (TS), dihydropyrimidine dehydrogenase (DPD) no hatsugen to CD-DST-ho (collagen gel droplet embedded culture drug sensitivity test) ni yoru 5-FU kanjusei shiken tono kanren ...", GAN TO KAGAKU RYOHO, vol. 28, no. 5, May 2001 (2001-05-01), pages 661 - 667, XP002960714
TAKECHI T. ET AL.: "Screening of differentially expressed genes in 5-fluorouracil-resistant human gastrointestinal tumor cells", JPN. J. CANCER RES., vol. 92, no. 6, June 2001 (2001-06-01), pages 696 - 703, XP002960715
ISHIKAWA Y. ET AL.: "Dihydropyrimidine dehydrogenase and messenger RNA levels in gastric cancer: possible predictor for sensitivity to 5-fluorouracil", JPN. J. CANCER RES., vol. 91, no. 1, 2000, pages 105 - 112, XP002960716
MATA J.F. ET AL.: "Role of the human concentrative nucleoside transporter (hCNT1) in the cytotoxic action of 5(prime)-deoxy-5-fluorouridine, an active intermediate metabolite of capecitabine, a novel oral anticancer drug", MOL. PHARMACOL., vol. 59, no. 6, June 2001 (2001-06-01), pages 1542 - 1548, XP002960717
METZGER R. ET AL.: "ERCC1 mRNA levels complement thymidylate synthase mRNA levels in predicting response and survival for gastric cancer patients receiving combination cisplatin and fluorouracil chemotherapy", J. CLIN. ONCOL., vol. 16, no. 1, 1998, pages 309 - 316, XP001118953
DUSSEAU C. ET AL.: "Analysis of uracil DNA glycosylase in human colorectal cancer", INT. J. ONCOL., vol. 18, no. 2, February 2001 (2001-02-01), pages 393 - 399, XP002960718
ZEMBUTSU H. ET AL.: "Genome-wide cDNA microarray screening to correlate gene expression profiles with sensitivity of 85 human cancer xenografts to anticancer drugs", CANCER RES., vol. 62, no. 2, January 2002 (2002-01-01), pages 518 - 527, XP002224797
KIHARA C. ET AL.: "Prediction of sensitivity of esophageal tumors to adjuvant chemotherapy by cDNA microarray analysis of gene-expression profiles", CANCER RES., vol. 61, no. 17, September 2001 (2001-09-01), pages 6474 - 6479, XP002960719
WANG W. ET AL.: "Pharmacogenomic dissection of resistance to thymidylate synthase inhibitors", CANCER RES., vol. 61, no. 14, July 2001 (2001-07-01), pages 5505 - 5510, XP002960720
TSUNODA T. ET AL.: "Diagnosis system of drug sensitivity of cancer using cDNA microarray and multivariate statistical anaylsis", FRONTIERS SCIENCE SERIES, vol. 30, 2000, pages 16 - 17, XP002960710
GREM J.L. ET AL.: "Thymidine kinase, thymidylate synthase and dihydropyrimidine dehydrogenase profiles of cell lines of the National Cancer Institute's Anticancer Frug Scteen", CLIN. CANCER RES., vol. 7, no. 4, April 2001 (2001-04-01), pages 999 - 1009, XP002960711
ISHIKAWA Y. ET AL.: "Dihydropyrimidine dehydrogenase activity and messenger RNA level may be related to the antitumor effect of 5-fluorouracil on human tumor xenografts in nude mice", CLIN. CANCER RES., vol. 5, no. 4, 1999, pages 883 - 889, XP002960712
SALONGA D. ET AL.: "Colorectal tumors responding to 5-fluorouracil have low gene expression levels of dihydropyrimidine dehydrogenase, thymidylate synthase and thymidine phosphorylase", CLIN. CANCER RES., vol. 6, no. 4, 2000, pages 1322 - 1327, XP002960713
TAKUMI OCHIAI ET AL.: "Daicho-gan ni okeru orotate phosphoribosyl transferase (OPRT), thymidine kinase (TS), dihydropyrimidine dehydrogenase (DPD) no hatsugen to CD-DST-ho (collagen gel droplet embedded culture drug sensitivity test) ni yoru 5-FU kanjusei shiken tono kanren ...", GAN TO KAGAKU RYOHO, vol. 28, no. 5, May 2001 (2001-05-01), pages 661 - 667, XP002960714
TAKECHI T. ET AL.: "Screening of differentially expressed genes in 5-fluorouracil-resistant human gastrointestinal tumor cells", JPN. J. CANCER RES., vol. 92, no. 6, June 2001 (2001-06-01), pages 696 - 703, XP002960715
ISHIKAWA Y. ET AL.: "Dihydropyrimidine dehydrogenase and messenger RNA levels in gastric cancer: possible predictor for sensitivity to 5-fluorouracil", JPN. J. CANCER RES., vol. 91, no. 1, 2000, pages 105 - 112, XP002960716
MATA J.F. ET AL.: "Role of the human concentrative nucleoside transporter (hCNT1) in the cytotoxic action of 5(prime)-deoxy-5-fluorouridine, an active intermediate metabolite of capecitabine, a novel oral anticancer drug", MOL. PHARMACOL., vol. 59, no. 6, June 2001 (2001-06-01), pages 1542 - 1548, XP002960717
METZGER R. ET AL.: "ERCC1 mRNA levels complement thymidylate synthase mRNA levels in predicting response and survival for gastric cancer patients receiving combination cisplatin and fluorouracil chemotherapy", J. CLIN. ONCOL., vol. 16, no. 1, 1998, pages 309 - 316, XP001118953
DUSSEAU C. ET AL.: "Analysis of uracil DNA glycosylase in human colorectal cancer", INT. J. ONCOL., vol. 18, no. 2, February 2001 (2001-02-01), pages 393 - 399, XP002960718
ZEMBUTSU H. ET AL.: "Genome-wide cDNA microarray screening to correlate gene expression profiles with sensitivity of 85 human cancer xenografts to anticancer drugs", CANCER RES., vol. 62, no. 2, January 2002 (2002-01-01), pages 518 - 527, XP002224797
KIHARA C. ET AL.: "Prediction of sensitivity of esophageal tumors to adjuvant chemotherapy by cDNA microarray analysis of gene-expression profiles", CANCER RES., vol. 61, no. 17, September 2001 (2001-09-01), pages 6474 - 6479, XP002960719
WANG W. ET AL.: "Pharmacogenomic dissection of resistance to thymidylate synthase inhibitors", CANCER RES., vol. 61, no. 14, July 2001 (2001-07-01), pages 5505 - 5510, XP002960720
Attorney, Agent or Firm:
The, Patent Corporate Body Aruga Patent Office (3-6 NIHONBASHININGYOCHO 1-CHOM, CHUO-KU Tokyo, JP)
Download PDF:
Previous Patent: EXPRESSION VECTORS ENCODING BACTERIOPHAGE SIGNAL PEPTIDES
Next Patent: METHOD OF AMPLIFYING NONCYCLIC NUCLEIC ACID FRAGMENT
Next Patent: METHOD OF AMPLIFYING NONCYCLIC NUCLEIC ACID FRAGMENT