Title:
DNA CAPABLE OF INDUCING OSTEOBLAST-SPECIFIC EXPRESSION, AND NUCLEOTIDE SEQUENCE FOR SAME
Document Type and Number:
WIPO Patent Application WO/2011/016561
Kind Code:
A1
Abstract:
Disclosed are: an enhancer which can induce osteoblast-specific expression; and use of the enhancer. Specifically disclosed is a fragment having a function of an enhancer in Runx2 gene and having a length of about 1.3 kb, particularly 0.34 kb, specifically an enhancer depicted in SEQ ID NO: 1, 3, 4 or 5. The enhancer can activate the transcription of an osteoblast-specific gene when bound to a promoter.
Inventors:
KOMORI TOSHIHISA (JP)
IZUMI SHINICHI (JP)
MIYAZAKI TOSHIHIRO (JP)
ROKUTANDA SATOSHI (JP)
KOMORI HISATO (JP)
LIU WENGUANG (CN)
IZUMI SHINICHI (JP)
MIYAZAKI TOSHIHIRO (JP)
ROKUTANDA SATOSHI (JP)
KOMORI HISATO (JP)
LIU WENGUANG (CN)
Application Number:
PCT/JP2010/063411
Publication Date:
February 10, 2011
Filing Date:
August 06, 2010
Export Citation:
Assignee:
UNIV NAGASAKI (JP)
KOMORI TOSHIHISA (JP)
IZUMI SHINICHI (JP)
MIYAZAKI TOSHIHIRO (JP)
ROKUTANDA SATOSHI (JP)
KOMORI HISATO (JP)
LIU WENGUANG (CN)
KOMORI TOSHIHISA (JP)
IZUMI SHINICHI (JP)
MIYAZAKI TOSHIHIRO (JP)
ROKUTANDA SATOSHI (JP)
KOMORI HISATO (JP)
LIU WENGUANG (CN)
International Classes:
C12N15/09; A01K67/027; A61K48/00; A61P1/02; A61P9/10; A61P19/00; A61P19/02; A61P19/08; A61P19/10; A61P35/00; A61P35/04; A61P43/00; C12N5/10; C12Q1/02
Foreign References:
| US20060242725A1 | 2006-10-26 |
Other References:
ZAMBOTTI A ET AL.: "Characterization of an osteoblast-specific enhancer element in the CBFA1 gene.", J BIOL CHEM, vol. 277, no. 44, 2002, pages 41497 - 41506
STRONG DD ET AL.: "The human type la2 procollagen nuclear targeting sequence together with human runx2 enhancer elements drives robust expression in transgenic mouse osteoblasts.", JOURNAL OF BONE AND MINERAL RESEARCH, vol. 22, no. 1, 2007, pages S80, 1288
THIRUNAVUKKARASU ET AL.: "Cryptic enhancer elements in luciferase reporter vectors respond to the osteoblast-specific transcription factor Osf2/Cbfal.", BIOTECHNIQUES, vol. 28, no. 3, 2000, pages 506 - 510
STRONG DD ET AL.: "The human type la2 procollagen nuclear targeting sequence together with human runx2 enhancer elements drives robust expression in transgenic mouse osteoblasts.", JOURNAL OF BONE AND MINERAL RESEARCH, vol. 22, no. 1, 2007, pages S80, 1288
THIRUNAVUKKARASU ET AL.: "Cryptic enhancer elements in luciferase reporter vectors respond to the osteoblast-specific transcription factor Osf2/Cbfal.", BIOTECHNIQUES, vol. 28, no. 3, 2000, pages 506 - 510
Attorney, Agent or Firm:
TAKASHIMA, HAJIME (JP)
Takashima 1 (JP)
Takashima 1 (JP)
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