Title:
DNA METHYLATION BIOMARKERS FOR CANCER DIAGNOSING AND TREATMENT
Document Type and Number:
WIPO Patent Application WO/2020/251851
Kind Code:
A3
Abstract:
Cancer is the second most common cause of death worldwide, identification of cancer-specific DNA methylation events released by tumors into blood can be used for cost-effective, minimally invasive diagnostics and monitoring of cancer. The present invention clinically tested a set of ten DNA methylation specific qPCR amplicons, designed to detect most common human carcinoma types, in cell free DNA extracted from plasma fraction of blood samples from healthy controls and non-small cell lung cancer (NSCLC) cases. The DNA methylation biomarkers distinguish lung cancer cases from controls with high sensitivity and specificity (AUC = 0.956), and furthermore, the signal from the markers depends on the tumor size and decreases after surgical resection of lung tumors. These observations indicate clinical value of these DNA methylation biomarkers for minimally invasive diagnostics and monitoring of NSCLC. It is predicted that these DNA methylation biomarkers will detect additional carcinoma types as well.
Inventors:
FUTSCHER BERNARD W (US)
VRBA LUKAS (US)
NELSON MARK A (US)
VRBA LUKAS (US)
NELSON MARK A (US)
Application Number:
PCT/US2020/036342
Publication Date:
January 28, 2021
Filing Date:
June 05, 2020
Export Citation:
Assignee:
UNIV ARIZONA (US)
International Classes:
A61P35/04; C12Q1/68; C12Q1/686; C12Q1/6886; G01N33/50
Domestic Patent References:
| WO2019068082A1 | 2019-04-04 |
Foreign References:
| US20090208930A1 | 2009-08-20 |
Other References:
VRBA LUKAS, OSHIRO MARC M., KIM SAMUEL S., GARLAND LINDA L., PLACENCIA CRYSTAL, MAHADEVAN DARUKA, NELSON MARK A., FUTSCHER BERNARD: "DNA methylation biomarkers discovered in silico detect cancer in liquid biopsies from non-small cell lung cancer patients", EPIGENETICS, LANDES BIOSCIENCE, US, vol. 15, no. 4, 27 November 2019 (2019-11-27), US, pages 419 - 430, XP055785561, ISSN: 1559-2294, DOI: 10.1080/15592294.2019.1695333
VRBA LUKAS, FUTSCHER BERNARD W.: "DNA methylation changes in biomarker loci occur early in cancer progression", F1000RESEARCH, vol. 8, 2106, 1 January 2019 (2019-01-01), pages 1 - 9, XP055785555, DOI: 10.12688/f1000research.21584.1
VRBA LUKAS, FUTSCHER BERNARD W.: "DNA methylation changes in biomarker loci occur early in cancer progression", F1000RESEARCH, vol. 8, 2106, 1 January 2019 (2019-01-01), pages 1 - 9, XP055785555, DOI: 10.12688/f1000research.21584.1
Attorney, Agent or Firm:
NGUYEN, Quan (US)
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