Title:
EFFICIENT MYOCARDIAL CELL INDUCTION METHOD
Document Type and Number:
WIPO Patent Application WO/2014/185358
Kind Code:
A1
Abstract:
The present invention provides a method for producing myocardial cells from pluripotent stem cells with high efficiency by including, in the method for producing myocardial cells from pluripotent stem cells, a process for forming embryoid bodies by dissociating embryoid bodies obtained partway through the production process and re-aggregating same.
Inventors:
YAMANAKA SHINYA (JP)
YOSHIDA YOSHINORI (JP)
MIKI KENJI (JP)
YOSHIDA YOSHINORI (JP)
MIKI KENJI (JP)
Application Number:
PCT/JP2014/062500
Publication Date:
November 20, 2014
Filing Date:
May 09, 2014
Export Citation:
Assignee:
UNIV KYOTO (JP)
International Classes:
C12N5/0735; C12N5/077; C12N5/10
Domestic Patent References:
| WO2012024782A1 | 2012-03-01 | |||
| WO2012108444A1 | 2012-08-16 | |||
| WO2007069666A1 | 2007-06-21 | |||
| WO2007002136A2 | 2007-01-04 | |||
| WO2009118928A1 | 2009-10-01 | |||
| WO2008118820A2 | 2008-10-02 | |||
| WO2009007852A2 | 2009-01-15 | |||
| WO2009032194A1 | 2009-03-12 | |||
| WO2009058413A1 | 2009-05-07 | |||
| WO2009057831A1 | 2009-05-07 | |||
| WO2009075119A1 | 2009-06-18 | |||
| WO2009079007A1 | 2009-06-25 | |||
| WO2009091659A2 | 2009-07-23 | |||
| WO2009101084A1 | 2009-08-20 | |||
| WO2009101407A2 | 2009-08-20 | |||
| WO2009102983A2 | 2009-08-20 | |||
| WO2009114949A1 | 2009-09-24 | |||
| WO2009117439A2 | 2009-09-24 | |||
| WO2009126250A2 | 2009-10-15 | |||
| WO2009126251A2 | 2009-10-15 | |||
| WO2009126655A2 | 2009-10-15 | |||
| WO2009157593A1 | 2009-12-30 | |||
| WO2010009015A2 | 2010-01-21 | |||
| WO2010033906A2 | 2010-03-25 | |||
| WO2010033920A2 | 2010-03-25 | |||
| WO2010042800A1 | 2010-04-15 | |||
| WO2010050626A1 | 2010-05-06 | |||
| WO2010056831A2 | 2010-05-20 | |||
| WO2010068955A2 | 2010-06-17 | |||
| WO2010098419A1 | 2010-09-02 | |||
| WO2010102267A2 | 2010-09-10 | |||
| WO2010111409A2 | 2010-09-30 | |||
| WO2010111422A2 | 2010-09-30 | |||
| WO2010115050A2 | 2010-10-07 | |||
| WO2010124290A2 | 2010-10-28 | |||
| WO2010147395A2 | 2010-12-23 | |||
| WO2010147612A1 | 2010-12-23 | |||
| WO2010008054A1 | 2010-01-21 | |||
| WO2010137746A1 | 2010-12-02 | |||
| WO2009123349A1 | 2009-10-08 | |||
| WO2010013845A1 | 2010-02-04 | |||
| WO2011007900A1 | 2011-01-20 | |||
| WO2009146408A1 | 2009-12-03 |
Foreign References:
| JP2008546413A | 2008-12-25 | |||
| JP2008546414A | 2008-12-25 | |||
| JP2012519005A | 2012-08-23 | |||
| JP2013507936A | 2013-03-07 | |||
| US5843780A | 1998-12-01 |
Other References:
DUBOIS, N.C. ET AL.: "SIRPA is a specific cell -surface marker for isolating cardiomyocytes derived from human pluripotent stem cells", NATURE BIOTECHNOLOGY, vol. 29, no. 11, 2011, pages 1011 - 1018, XP009167979
YANG, L. ET AL.: "Human cardiovascular progenitor cells develop from a KDR+ embryonic- stem- cell -derived population", NATURE, vol. 453, 2008, pages 524 - 528, XP002561095
IGLESIAS-GARCIA, O. ET AL.: "Induced pluripotent stem cells as a new strategy for cardiac regeneration and disease modeling", JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, vol. 62, 30 April 2013 (2013-04-30), pages 43 - 50, XP055295217
WILLIEMS, E. ET AL.: "Small-Molecule Inhibitors of the Wnt Pathway Potently Promote Cardiomyocytes From Human Embryonic Stem Cell Derived Mesoderm", CIRC RES., vol. 109, 2011, pages 360 - 364, XP055080723
CHEN, B. ET AL.: "Small molecule.mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer", NATURE CHEMICAL BIOLOGY, vol. 5, no. 2, 2009, pages 100 - 107, XP055295218
KATTMAN, S.J. ET AL.: "Stage-Specific Optimization of Activin/Nodal and BMP Signaling Promotes Cardiac Differentiation of Mouse and Human Pluripotent Stem Cell Lines", CELL STEM CELL, vol. 8, 2011, pages 228 - 240, XP055008193
YAN P ET AL., BIOCHEM BIOPHYS RES COMMUN., vol. 379, 2009, pages 115 - 20
LAFLAMME MA ET AL., NAT BIOTECHNOL, vol. 25, 2007, pages 1015 - 1024
YANG L ET AL., NATURE, vol. 453, 2008, pages 524 - 528
M. J. EVANS; M. H. KAUFMAN, NATURE, vol. 292, 1981, pages 154 - 156
J. A. THOMSON ET AL., SCIENCE, vol. 282, 1998, pages 1145 - 1147
J. A. THOMSON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 92, 1995, pages 7844 - 7848
J. A. THOMSON ET AL., BIOL. REPROD., vol. 55, 1996, pages 254 - 259
J. A. THOMSON; V. S. MARSHALL, CURR. TOP. DEV. BIOL., vol. 38, 1998, pages 133 - 165
THOMSON JA ET AL., PROC NATL. ACAD. SCI. USA., vol. 92, 1995, pages 7844 - 7848
THOMSON JA ET AL., SCIENCE, vol. 282, 1998, pages 1145 - 1147
H. SUEMORI ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 345, 2006, pages 926 - 932
M. UENO ET AL., PROC. NATL. ACAD. SCI. USA, vol. 103, 2006, pages 9554 - 9559
H. SUEMORI ET AL., DEV. DYN., vol. 222, 2001, pages 273 - 279
H. KAWASAKI ET AL., PROC. NATL. ACAD. SCI. USA, vol. 99, 2002, pages 1580 - 1585
KLIMANSKAYA I ET AL., NATURE, vol. 444, 2006, pages 481 - 485
E. KROON ET AL., NAT. BIOTECHNOL., vol. 26, 2008, pages 443 - 452
M. KANATSU-SHINOHARA ET AL., BIOL. REPROD., vol. 69, 2003, pages 612 - 616
K. SHINOHARA ET AL., CELL, vol. 119, 2004, pages 1001 - 1012
MASANORI TAKEHASHI ET AL.: "Experimental Medicine", vol. 26, 2008, YODOSHA, pages: 41 - 46
Y. MATSUI ET AL., CELL, vol. 70, 1992, pages 841 - 847
J. L. RESNICK ET AL., NATURE, vol. 359, 1992, pages 550 - 551
K. TAKAHASHI; S. YAMANAKA, CELL, vol. 126, 2006, pages 663 - 676
K. TAKAHASHI ET AL., CELL, vol. 131, 2007, pages 861 - 872
J. YU ET AL., SCIENCE, vol. 318, 2007, pages 1917 - 1920
NAKAGAWA, M. ET AL., NAT. BIOTECHNOL., vol. 26, 2008, pages 101 - 106
HUANGFU D ET AL., NAT. BIOTECHNOL., vol. 26, 2008, pages 795 - 797
SHI Y ET AL., CELL STEM CELL, vol. 2, 2008, pages 525 - 528
EMINLI S ET AL., STEM CELLS, vol. 26, 2008, pages 2467 - 2474
HUANGFU D ET AL., NAT BIOTECHNOL., vol. 26, 2008, pages 1269 - 1275
SHI Y ET AL., CELL STEM CELL, vol. 3, 2008, pages 568 - 574
ZHAO Y ET AL., CELL STEM CELL, vol. 3, 2008, pages 475 - 479
MARSON A, CELL STEM CELL, vol. 3, 2008, pages 132 - 135
FENG B ET AL., NAT CELL BIOL., vol. 11, 2009, pages 197 - 203
R.L. JUDSON ET AL., NAT. BIOTECH., vol. 27, 2009, pages 459 - 461
LYSSIOTIS CA ET AL., PROC NATL ACAD SCI USA., vol. 106, 2009, pages 8912 - 8917
KIM JB ET AL., NATURE, vol. 461, 2009, pages 649 - 643
ICHIDA JK ET AL., CELL STEM CELL., vol. 5, 2009, pages 491 - 503
HENG JC ET AL., CELL STEM CELL., vol. 6, 2010, pages 167 - 74
HAN J ET AL., NATURE, vol. 463, 2010, pages 1096 - 100
MALI P ET AL., STEM CELLS., vol. 28, 2010, pages 713 - 720
MAEKAWA M ET AL., NATURE, vol. 474, 2011, pages 225 - 9
CELL, vol. 126, 2006, pages 663 - 676
CELL, vol. 131, 2007, pages 861 - 872
SCIENCE, vol. 318, 2007, pages 1917 - 1920
SCIENCE, vol. 322, 2008, pages 945 - 949
SCIENCE, vol. 322, 2008, pages 949 - 953
WARREN L, CELL STEM CELL, vol. 7, 2010, pages 618 - 630
TAKAHASHI K ET AL., PLOS ONE., vol. 4, 2009, pages E8067
SUN N ET AL., PROC NATL ACAD SCI U S A., vol. 106, 2009, pages 15720 - 15725
YOSHIDA Y ET AL., CELL STEM CELL, vol. 5, 2009, pages 237 - 241
T. WAKAYAMA ET AL., SCIENCE, vol. 292, 2001, pages 740 - 743
S. WAKAYAMA ET AL., BIOL. REPROD., vol. 72, 2005, pages 932 - 936
J. BYRNE ET AL., NATURE, vol. 450, 2007, pages 497 - 502
J. B. CIBELLI ET AL., NATURE BIOTECHNOL., vol. 16, 1998, pages 642 - 646
SAYAKA WAKAYAMA ET AL., EXPERIMENTAL MEDICINE, vol. 26, no. 5, 2008, pages 47 - 52
P. B. YU ET AL., CIRCULATION, vol. 116, 2007
P.B. YU ET AL., NAT. CHEM. BIOL., vol. 4, 2008, pages 33 - 41
J. HAO ET AL., PLOS ONE, vol. 3, no. 8, 2008, pages E2904
R. K. LINDEMANN ET AL., MOL. CANCER, vol. 2, 2003, pages 20
TAKAHASHI K ET AL., CELL, vol. 131, 2007, pages 861 - 72
OKITA. K ET AL., STEM CELLS., 29 November 2012 (2012-11-29)
TAKAHASHI K ET AL., CELL., vol. 131, 2007, pages 861 - 72
NAKAGAWA M ET AL., NAT BIOTECHNOL., vol. 26, 2008, pages 101 - 6
OKITA ET AL., NAT METHODS, vol. 8, no. 5, 2011, pages 409 - 12
OKITA ET AL., STEM CELLS, vol. 31, no. 3, 2013, pages 458 - 66
KAJIWARA ET AL., PROC NATL ACAD SCI USA., vol. 109, no. 31, 2012, pages 12538 - 43
SUEMORI H ET AL., BIOCHEM BIOPHYS RES COMMUN., vol. 345, 2006, pages 926 - 32
YANG, L. ET AL.: "Human cardiovascular progenitor cells develop from a KDR+ embryonic- stem- cell -derived population", NATURE, vol. 453, 2008, pages 524 - 528, XP002561095
IGLESIAS-GARCIA, O. ET AL.: "Induced pluripotent stem cells as a new strategy for cardiac regeneration and disease modeling", JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, vol. 62, 30 April 2013 (2013-04-30), pages 43 - 50, XP055295217
WILLIEMS, E. ET AL.: "Small-Molecule Inhibitors of the Wnt Pathway Potently Promote Cardiomyocytes From Human Embryonic Stem Cell Derived Mesoderm", CIRC RES., vol. 109, 2011, pages 360 - 364, XP055080723
CHEN, B. ET AL.: "Small molecule.mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer", NATURE CHEMICAL BIOLOGY, vol. 5, no. 2, 2009, pages 100 - 107, XP055295218
KATTMAN, S.J. ET AL.: "Stage-Specific Optimization of Activin/Nodal and BMP Signaling Promotes Cardiac Differentiation of Mouse and Human Pluripotent Stem Cell Lines", CELL STEM CELL, vol. 8, 2011, pages 228 - 240, XP055008193
YAN P ET AL., BIOCHEM BIOPHYS RES COMMUN., vol. 379, 2009, pages 115 - 20
LAFLAMME MA ET AL., NAT BIOTECHNOL, vol. 25, 2007, pages 1015 - 1024
YANG L ET AL., NATURE, vol. 453, 2008, pages 524 - 528
M. J. EVANS; M. H. KAUFMAN, NATURE, vol. 292, 1981, pages 154 - 156
J. A. THOMSON ET AL., SCIENCE, vol. 282, 1998, pages 1145 - 1147
J. A. THOMSON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 92, 1995, pages 7844 - 7848
J. A. THOMSON ET AL., BIOL. REPROD., vol. 55, 1996, pages 254 - 259
J. A. THOMSON; V. S. MARSHALL, CURR. TOP. DEV. BIOL., vol. 38, 1998, pages 133 - 165
THOMSON JA ET AL., PROC NATL. ACAD. SCI. USA., vol. 92, 1995, pages 7844 - 7848
THOMSON JA ET AL., SCIENCE, vol. 282, 1998, pages 1145 - 1147
H. SUEMORI ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 345, 2006, pages 926 - 932
M. UENO ET AL., PROC. NATL. ACAD. SCI. USA, vol. 103, 2006, pages 9554 - 9559
H. SUEMORI ET AL., DEV. DYN., vol. 222, 2001, pages 273 - 279
H. KAWASAKI ET AL., PROC. NATL. ACAD. SCI. USA, vol. 99, 2002, pages 1580 - 1585
KLIMANSKAYA I ET AL., NATURE, vol. 444, 2006, pages 481 - 485
E. KROON ET AL., NAT. BIOTECHNOL., vol. 26, 2008, pages 443 - 452
M. KANATSU-SHINOHARA ET AL., BIOL. REPROD., vol. 69, 2003, pages 612 - 616
K. SHINOHARA ET AL., CELL, vol. 119, 2004, pages 1001 - 1012
MASANORI TAKEHASHI ET AL.: "Experimental Medicine", vol. 26, 2008, YODOSHA, pages: 41 - 46
Y. MATSUI ET AL., CELL, vol. 70, 1992, pages 841 - 847
J. L. RESNICK ET AL., NATURE, vol. 359, 1992, pages 550 - 551
K. TAKAHASHI; S. YAMANAKA, CELL, vol. 126, 2006, pages 663 - 676
K. TAKAHASHI ET AL., CELL, vol. 131, 2007, pages 861 - 872
J. YU ET AL., SCIENCE, vol. 318, 2007, pages 1917 - 1920
NAKAGAWA, M. ET AL., NAT. BIOTECHNOL., vol. 26, 2008, pages 101 - 106
HUANGFU D ET AL., NAT. BIOTECHNOL., vol. 26, 2008, pages 795 - 797
SHI Y ET AL., CELL STEM CELL, vol. 2, 2008, pages 525 - 528
EMINLI S ET AL., STEM CELLS, vol. 26, 2008, pages 2467 - 2474
HUANGFU D ET AL., NAT BIOTECHNOL., vol. 26, 2008, pages 1269 - 1275
SHI Y ET AL., CELL STEM CELL, vol. 3, 2008, pages 568 - 574
ZHAO Y ET AL., CELL STEM CELL, vol. 3, 2008, pages 475 - 479
MARSON A, CELL STEM CELL, vol. 3, 2008, pages 132 - 135
FENG B ET AL., NAT CELL BIOL., vol. 11, 2009, pages 197 - 203
R.L. JUDSON ET AL., NAT. BIOTECH., vol. 27, 2009, pages 459 - 461
LYSSIOTIS CA ET AL., PROC NATL ACAD SCI USA., vol. 106, 2009, pages 8912 - 8917
KIM JB ET AL., NATURE, vol. 461, 2009, pages 649 - 643
ICHIDA JK ET AL., CELL STEM CELL., vol. 5, 2009, pages 491 - 503
HENG JC ET AL., CELL STEM CELL., vol. 6, 2010, pages 167 - 74
HAN J ET AL., NATURE, vol. 463, 2010, pages 1096 - 100
MALI P ET AL., STEM CELLS., vol. 28, 2010, pages 713 - 720
MAEKAWA M ET AL., NATURE, vol. 474, 2011, pages 225 - 9
CELL, vol. 126, 2006, pages 663 - 676
CELL, vol. 131, 2007, pages 861 - 872
SCIENCE, vol. 318, 2007, pages 1917 - 1920
SCIENCE, vol. 322, 2008, pages 945 - 949
SCIENCE, vol. 322, 2008, pages 949 - 953
WARREN L, CELL STEM CELL, vol. 7, 2010, pages 618 - 630
TAKAHASHI K ET AL., PLOS ONE., vol. 4, 2009, pages E8067
SUN N ET AL., PROC NATL ACAD SCI U S A., vol. 106, 2009, pages 15720 - 15725
YOSHIDA Y ET AL., CELL STEM CELL, vol. 5, 2009, pages 237 - 241
T. WAKAYAMA ET AL., SCIENCE, vol. 292, 2001, pages 740 - 743
S. WAKAYAMA ET AL., BIOL. REPROD., vol. 72, 2005, pages 932 - 936
J. BYRNE ET AL., NATURE, vol. 450, 2007, pages 497 - 502
J. B. CIBELLI ET AL., NATURE BIOTECHNOL., vol. 16, 1998, pages 642 - 646
SAYAKA WAKAYAMA ET AL., EXPERIMENTAL MEDICINE, vol. 26, no. 5, 2008, pages 47 - 52
P. B. YU ET AL., CIRCULATION, vol. 116, 2007
P.B. YU ET AL., NAT. CHEM. BIOL., vol. 4, 2008, pages 33 - 41
J. HAO ET AL., PLOS ONE, vol. 3, no. 8, 2008, pages E2904
R. K. LINDEMANN ET AL., MOL. CANCER, vol. 2, 2003, pages 20
TAKAHASHI K ET AL., CELL, vol. 131, 2007, pages 861 - 72
OKITA. K ET AL., STEM CELLS., 29 November 2012 (2012-11-29)
TAKAHASHI K ET AL., CELL., vol. 131, 2007, pages 861 - 72
NAKAGAWA M ET AL., NAT BIOTECHNOL., vol. 26, 2008, pages 101 - 6
OKITA ET AL., NAT METHODS, vol. 8, no. 5, 2011, pages 409 - 12
OKITA ET AL., STEM CELLS, vol. 31, no. 3, 2013, pages 458 - 66
KAJIWARA ET AL., PROC NATL ACAD SCI USA., vol. 109, no. 31, 2012, pages 12538 - 43
SUEMORI H ET AL., BIOCHEM BIOPHYS RES COMMUN., vol. 345, 2006, pages 926 - 32
Attorney, Agent or Firm:
KAWAGUCHI, Yoshiyuki et al. (JP)
Yoshiyuki Kawaguchi (JP)
Yoshiyuki Kawaguchi (JP)
Download PDF: