Title:
ELECTROTRANSFORMATION OF GRAM-POSITIVE, ANAEROBIC, THERMOPHILIC BACTERIA
Document Type and Number:
WIPO Patent Application WO/2010/056450
Kind Code:
A3
Abstract:
The present invention relates to methods for transforming Gram-positive, anaerobic, thermophilic bacteria via electroporation and Gram-positive, anaerobic, thermophilic bacteria transformed by the disclosed methods. The methods employ voltage pulsing schemes that decrease arcing such that increased transformation efficiency and cell viability is observed. The present invention is further directed to a method for transforming Gram-positive, anaerobic, thermophilic bacteria via electroporation using recovery/selection temperatures to effect increased transformation efficiency in difficult to transform bacteria.
More Like This:
Inventors:
OLSON DANIEL G (US)
Application Number:
PCT/US2009/060501
Publication Date:
August 19, 2010
Filing Date:
October 13, 2009
Export Citation:
Assignee:
MASCOMA CORP (US)
OLSON DANIEL G (US)
OLSON DANIEL G (US)
International Classes:
C12N15/00; C12N15/09; C12N15/64
Domestic Patent References:
| WO2005116203A2 | 2005-12-08 |
Other References:
KUSAOKE H ET AL.: "An Improved Method for DNA Transformation of Intact Cells of Bacillus subtilis by Electroporation", MEMOIRS OF FUKUI INSTITUTE OF TECHNOLOGY, vol. 22, no. 1, 1 January 1992 (1992-01-01), pages 223 - 233, XP002570241
TYURIN MICHAEL V ET AL: "Electrotransformation of Clostridium thermocellum", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 70, no. 2, 1 February 2004 (2004-02-01), pages 883 - 890, XP002352430, ISSN: 0099-2240
TARDIF C ET AL: "Electrotransformation studies in Clostridium cellulolyticum", JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY, BASINGSTOKE, GB, vol. 27, no. 5, 1 November 2001 (2001-11-01), pages 271 - 274, XP009129780, ISSN: 1367-5435
JENNERT K C B ET AL: "GENE TRANSFER TO CLOSTRIDIUM CELLULOLYTICUM ATCC35319", MICROBIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, GB, vol. 146, 1 January 2000 (2000-01-01), pages 3071 - 3080, XP002515778, ISSN: 1350-0872
TYURIN M ET AL: "Electrotransformation of Clostridium acetobutylicum ATCC 824 using high-voltage radio frequency modulated square pulses", JOURNAL OF APPLIED MICROBIOLOGY, OXFORD, GB, vol. 88, no. 2, 1 February 2000 (2000-02-01), pages 220 - 227, XP002352429, ISSN: 1364-5072
LURQUIN P F: "GENE TRANSFER BY ELECTROPORATION", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, HUMANA PRESS, INC, US, vol. 7, no. 1, 1 January 1997 (1997-01-01), pages 5 - 35, XP001073618, ISSN: 0273-2289
TYURIN MICHAEL V ET AL: "Electrotransformation of Clostridium thermocellum", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 70, no. 2, 1 February 2004 (2004-02-01), pages 883 - 890, XP002352430, ISSN: 0099-2240
TARDIF C ET AL: "Electrotransformation studies in Clostridium cellulolyticum", JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY, BASINGSTOKE, GB, vol. 27, no. 5, 1 November 2001 (2001-11-01), pages 271 - 274, XP009129780, ISSN: 1367-5435
JENNERT K C B ET AL: "GENE TRANSFER TO CLOSTRIDIUM CELLULOLYTICUM ATCC35319", MICROBIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, GB, vol. 146, 1 January 2000 (2000-01-01), pages 3071 - 3080, XP002515778, ISSN: 1350-0872
TYURIN M ET AL: "Electrotransformation of Clostridium acetobutylicum ATCC 824 using high-voltage radio frequency modulated square pulses", JOURNAL OF APPLIED MICROBIOLOGY, OXFORD, GB, vol. 88, no. 2, 1 February 2000 (2000-02-01), pages 220 - 227, XP002352429, ISSN: 1364-5072
LURQUIN P F: "GENE TRANSFER BY ELECTROPORATION", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, HUMANA PRESS, INC, US, vol. 7, no. 1, 1 January 1997 (1997-01-01), pages 5 - 35, XP001073618, ISSN: 0273-2289
Attorney, Agent or Firm:
JACKMAN, Peter, A. et al. (Kessler Goldstein & Fox P.L.L.C.,1100 New York Avenue, N.W, Washington DC, US)
Download PDF: