Title:
AN ELP FUSION PROTEIN AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2010/063172
Kind Code:
A1
Abstract:
Provided are a fusion protein comprising ELP and uses thereof, and ELP-tagged protease that can be used as recycling utilized enzyme with immobilized enzyme characteric and uses thereof. For example, the purification or seperation method using of phase transition characteristic of ELP comprises contacting the ELP fusion protein having protease cleavage site with ELP-tagged enzyme, then subjecting the cleavage mixture to phase transition mediated by changing temperature, pH or salt ionic concentration to recover the protein of interest from the cleavage mixture.
Inventors:
XU ANLONG (CN)
LAN DONGMING (CN)
HUANG GUANGRUI (CN)
WANG LEI (CN)
CHEN SHANGWU (CN)
LAN DONGMING (CN)
HUANG GUANGRUI (CN)
WANG LEI (CN)
CHEN SHANGWU (CN)
Application Number:
PCT/CN2009/072395
Publication Date:
June 10, 2010
Filing Date:
June 23, 2009
Export Citation:
Assignee:
UNIV SUN YAT SEN (CN)
XU ANLONG (CN)
LAN DONGMING (CN)
HUANG GUANGRUI (CN)
WANG LEI (CN)
CHEN SHANGWU (CN)
XU ANLONG (CN)
LAN DONGMING (CN)
HUANG GUANGRUI (CN)
WANG LEI (CN)
CHEN SHANGWU (CN)
International Classes:
C07K1/12; C07K19/00; C12N9/50; C12N15/62
Domestic Patent References:
| WO2002074928A2 | 2002-09-26 | |||
| WO2008030968A2 | 2008-03-13 |
Foreign References:
| US20080032931A1 | 2008-02-07 | |||
| CN101418290A | 2009-04-29 |
Other References:
LIM D.W. ET AL.: "Improved non-chromatographic purification of a recombinant protein by cationic elastin-like polypeptides", BIOMACROMOLECULES, vol. 8, no. 5, 2007, pages 1417 - 1424
HYE-JIN K. ET AL.: "Heterologous expression and optimized one-step separation oflevansucrase via elastin-like polypeptides tagging system", J. MICROBIOL. BIOTECHNOL., vol. 17, no. 11, 2007, pages 1751 - 1757
HYE-JIN K. ET AL.: "Heterologous expression and optimized one-step separation oflevansucrase via elastin-like polypeptides tagging system", J. MICROBIOL. BIOTECHNOL., vol. 17, no. 11, 2007, pages 1751 - 1757
Attorney, Agent or Firm:
SCIHEAD PATENT AGENT CO., LTD (Huihua Commercial & Trade BuildingNo.80 Xianliezhong Road, Yuexiu,Guangzhou, Guangdong 0, CN)
Download PDF: