Title:
EMBRYO CULTURE METHOD
Document Type and Number:
WIPO Patent Application WO/2023/182287
Kind Code:
A1
Abstract:
The present invention provides an embryo culture method. More specifically, the invention includes culturing an embryo (especially pre-implantation) on a dish having, on the surface thereof, an extracellular matrix layer having a sufficient thickness. The invention enables, for example, development of an embryo into a post-implantation embryo.
Inventors:
MENO CHIKARA (JP)
NII TAKENOBU (JP)
NII TAKENOBU (JP)
Application Number:
PCT/JP2023/010943
Publication Date:
September 28, 2023
Filing Date:
March 20, 2023
Export Citation:
Assignee:
UNIV KYUSHU NAT UNIV CORP (JP)
International Classes:
C12N5/073; C12M1/00; C12Q1/02
Foreign References:
| JP2020130055A | 2020-08-31 |
Other References:
GU ZHEN, GUO JIA, ZHAI JINGLEI, FENG GUIHAI, WANG XIANNING, GAO ZILI, JIANG LIYUAN, WEN YONGQIANG, WANG LIU, WEI LI, WANG SHUTAO, : "A uterus-inspired 3D niche drives embryo development beyond implantation", RESEARCH SQUARE, 3 December 2020 (2020-12-03), XP093095069, Retrieved from the Internet [retrieved on 20231025], DOI: 10.21203/rs.3.rs-121209/v1
MORRIS, SAMANTHA A. ET AL.: "Dynamics of anterior-posterior axis formation in the developing mouse embryo", NATURE COMMUNICATIONS, vol. 3, 2012, XP002681405, DOI: 10.1038/ncomms1671
AKIZAWA HIROKI, SAITO SHUN, KOHRI NANAMI, FURUKAWA ERI, HAYASHI YOSHIHIRO, BAI HANAKO, NAGANO MASASHI, YANAGAWA YOJIRO, TSUKAHARA : "Deciphering two rounds of cell lineage segregations during bovine preimplantation development", THE FASEB JOURNAL, FEDERATION OF AMERICAN SOCIETIES FOR EXPERIMENTAL BIOLOGY, US, vol. 35, no. 10, 1 October 2021 (2021-10-01), US, XP093095071, ISSN: 0892-6638, DOI: 10.1096/fj.202002762RR
TAKAYA ABE: "Development of an in vitro implantation model using morula organoids ", SEARCH RESEARCH PROJECTS I FY2020 RESEARCH REPORT; 19K18685, 27 December 2021 (2021-12-27), XP093095073, Retrieved from the Internet [retrieved on 20231025]
KAWAHARA MANABU: "Elucidation of the cell differentiation mechanism through the development of a new bovine fertilized egg culture system - Expectations for the development of animal husbandry from elucidation of the basic design of cattle ", RESEARCH FACULTY OF AGRICULTURE, 14 October 2021 (2021-10-14), XP093095076, Retrieved from the Internet [retrieved on 20231025]
BEDZHOV, IVAN ET AL.: "In vitro culture of mouse blastocysts beyond the implantation stages", NATURE PROTOCOLS, vol. 9, 2014, pages 2732 - 2739, XP055930068, DOI: 10.1038/nprot.2014.186
AGUILERA-CASTREJON, ALEJANDRO ET AL.: "Ex utero mouse embryogenesis from pre- gastrulation to late organogenesis", NATURE, vol. 593, 2021, pages 119 - 124, XP037443359, DOI: 10.1038/ s 41586-021 -03416-3
MORRIS, SAMANTHA A. ET AL.: "Dynamics of anterior-posterior axis formation in the developing mouse embryo", NATURE COMMUNICATIONS, vol. 3, 2012, XP002681405, DOI: 10.1038/ncomms1671
AKIZAWA HIROKI, SAITO SHUN, KOHRI NANAMI, FURUKAWA ERI, HAYASHI YOSHIHIRO, BAI HANAKO, NAGANO MASASHI, YANAGAWA YOJIRO, TSUKAHARA : "Deciphering two rounds of cell lineage segregations during bovine preimplantation development", THE FASEB JOURNAL, FEDERATION OF AMERICAN SOCIETIES FOR EXPERIMENTAL BIOLOGY, US, vol. 35, no. 10, 1 October 2021 (2021-10-01), US, XP093095071, ISSN: 0892-6638, DOI: 10.1096/fj.202002762RR
TAKAYA ABE: "Development of an in vitro implantation model using morula organoids ", SEARCH RESEARCH PROJECTS I FY2020 RESEARCH REPORT; 19K18685, 27 December 2021 (2021-12-27), XP093095073, Retrieved from the Internet
KAWAHARA MANABU: "Elucidation of the cell differentiation mechanism through the development of a new bovine fertilized egg culture system - Expectations for the development of animal husbandry from elucidation of the basic design of cattle ", RESEARCH FACULTY OF AGRICULTURE, 14 October 2021 (2021-10-14), XP093095076, Retrieved from the Internet
BEDZHOV, IVAN ET AL.: "In vitro culture of mouse blastocysts beyond the implantation stages", NATURE PROTOCOLS, vol. 9, 2014, pages 2732 - 2739, XP055930068, DOI: 10.1038/nprot.2014.186
AGUILERA-CASTREJON, ALEJANDRO ET AL.: "Ex utero mouse embryogenesis from pre- gastrulation to late organogenesis", NATURE, vol. 593, 2021, pages 119 - 124, XP037443359, DOI: 10.1038/ s 41586-021 -03416-3
Attorney, Agent or Firm:
MORITA Yutaka (JP)
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