Title:
ENGINEERED KETOREDUCTASES FOR THE PREPARATION OF CHIRAL ALCOHOLS AND METHODS THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/001593
Kind Code:
A1
Abstract:
Provided is an engineered ketoreductase polypeptide capable of preparing chiral alcohols, especially for catalyzing the reduction of ethyl 4-chloroacetoacetate to produce ethyl (R) -4-chloro-3-hydroxybutyrate. The polypeptides has high catalytic activity, high stereoselectivity, better thermal stability and solvent tolerance; the reaction process using engineered ketoreductase polypeptides may have a substrate loading of up to 426 g/L and a conversion of up to 99%.
Inventors:
CHEN HAIBIN (CN)
LIANG YAFANG (CN)
ZHANG YINGXIN (CN)
XIA ZEKUN (CN)
ZHOU AMENG (CN)
BOCOLA MARCO (CN)
XU JUNPENG (CN)
LUO XIAO (CN)
MAO JINGXIAN (CN)
HONG RUIMEI (CN)
WU XINWEI (CN)
SUN LEI (CN)
DAI YIXIN (CN)
WANG SONGXUE (CN)
PENG QINLI (CN)
LIANG YAFANG (CN)
ZHANG YINGXIN (CN)
XIA ZEKUN (CN)
ZHOU AMENG (CN)
BOCOLA MARCO (CN)
XU JUNPENG (CN)
LUO XIAO (CN)
MAO JINGXIAN (CN)
HONG RUIMEI (CN)
WU XINWEI (CN)
SUN LEI (CN)
DAI YIXIN (CN)
WANG SONGXUE (CN)
PENG QINLI (CN)
Application Number:
PCT/CN2023/094905
Publication Date:
January 04, 2024
Filing Date:
May 17, 2023
Export Citation:
Assignee:
ENZYMASTER NINGBO BIO ENG CO LTD (CN)
International Classes:
C12N9/04; C12N1/21; C12N11/00; C12N15/53; C12P7/18; C12P7/22; C12P7/62; C12P41/00; C12R1/19
Foreign References:
| CN103923957A | 2014-07-16 | |||
| US20170226546A1 | 2017-08-10 | |||
| CN101437948A | 2009-05-20 | |||
| CN111321129A | 2020-06-23 | |||
| CN103276027A | 2013-09-04 | |||
| CN101473027A | 2009-07-01 |
Other References:
NOBUYUKI URANO; SATOKO FUKUI; SHOKO KUMASHIRO; TAKERU ISHIGE; SHINJI KITA; KEIJI SAKAMOTO; MICHIHIKO KATAOKA; SAKAYU SHIMIZU;: "Directed evolution of an aminoalcohol dehydrogenase for efficient production of double chiral aminoalcohols", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, ELSEVIER, AMSTERDAM, NL, vol. 111, no. 3, 12 November 2010 (2010-11-12), NL , pages 266 - 271, XP028149092, ISSN: 1389-1723, DOI: 10.1016/j.jbiosc.2010.11.005
SCHENKELS P, DUINE J A: "Nicotinoprotein (NADH-containing) alcohol dehydrogenase from Rhodococcus erythropolis DSM 1069: An efficient catalyst for coenzyme-independent oxidation of a broad spectrum of alcohols and the interconversion of alcohols and aldehydes.", MICROBIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, vol. 146, no. 4, 1 April 2000 (2000-04-01), Reading , pages 775 - 785, XP002253322, ISSN: 1350-0872
SCHENKELS P, DUINE J A: "Nicotinoprotein (NADH-containing) alcohol dehydrogenase from Rhodococcus erythropolis DSM 1069: An efficient catalyst for coenzyme-independent oxidation of a broad spectrum of alcohols and the interconversion of alcohols and aldehydes.", MICROBIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, vol. 146, no. 4, 1 April 2000 (2000-04-01), Reading , pages 775 - 785, XP002253322, ISSN: 1350-0872
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