Title:
ENGINEERED POLYPEPTIDES AND THEIR APPLICATIONS IN SYNTHESIS OF BETA-HYDROXY-ALPHA-AMINO ACIDS
Document Type and Number:
WIPO Patent Application WO/2018/219107
Kind Code:
A1
Abstract:
Provided are engineered polypeptides that are useful for the asymmetric synthesis of β-hydroxy-α-amino acids under industrial-relevant conditions. The engineered polypeptides disclosed are developed through directed evolution based on the ability of catalytic synthesis of (2S, 3R) -2-amino-3-hydroxy-3- (4-nitrophenyl) propanoic acid. Also provided are polynucleotides encoding the engineered polypeptides, host cells capable of expressing engineered polypeptides, and methods of producing β-hydroxy-α-amino acids using engineered polypeptides. Compared to other processes of preparation, the use of the engineered polypeptides for the preparation of β-hydroxy-α-amino acids results in high purity of the desired stereoisomers, mild reaction conditions, low pollution and low energy consumption. It has good industrial application prospects.
Inventors:
CHEN HAIBIN (CN)
BONG YONG KOY (CN)
XU QING (CN)
ZHOU AMENG (CN)
SHEN TIANRAN (CN)
YANG JIADONG (CN)
YANG ZHUHONG (CN)
BONG YONG KOY (CN)
XU QING (CN)
ZHOU AMENG (CN)
SHEN TIANRAN (CN)
YANG JIADONG (CN)
YANG ZHUHONG (CN)
Application Number:
PCT/CN2018/086227
Publication Date:
December 06, 2018
Filing Date:
May 09, 2018
Export Citation:
Assignee:
ENZYMASTER NINGBO BIO ENG CO LTD (CN)
International Classes:
C07K14/21; C12P13/04; C12P41/00
Foreign References:
| JP2006121983A | 2006-05-18 | |||
| CA2648697A1 | 2007-10-25 | |||
| EP0460883A2 | 1991-12-11 | |||
| CN104673814A | 2015-06-03 |
Other References:
DATABASE GenBank [O] 10 August 2005 (2005-08-10), "phenylserine aldolase [Pseudomonas putida", XP055556916, Database accession no. BAD91544.1
GWON, H.J. ET AL.: "Diastereoselective synthesis of L-threo-3, 4-dihydroxyphenylserine by low-specific L-threonine aldolase mutants", BIOTECHNOL. LETT., vol. 32, no. 1, 17 September 2009 (2009-09-17), pages 143 - 149, XP019766769
FESKO, K.: "Threonine aldolases: perspectives in engineering and screening the enzymes with enhanced substrate and stereo specificities", APPL. MICROBIOL. BIOTECHNOL., vol. 100, no. 6, 26 January 2016 (2016-01-26), pages 2579 - 2590, XP035631555
LEE, S.J. ET AL.: "High-throughput screening methods for selecting 1-threonine aldolases with improved activity", JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC, vol. 26, no. 3-6, 31 December 2003 (2003-12-31), pages 265 - 272, XP002992768
See also references of EP 3630798A4
GWON, H.J. ET AL.: "Diastereoselective synthesis of L-threo-3, 4-dihydroxyphenylserine by low-specific L-threonine aldolase mutants", BIOTECHNOL. LETT., vol. 32, no. 1, 17 September 2009 (2009-09-17), pages 143 - 149, XP019766769
FESKO, K.: "Threonine aldolases: perspectives in engineering and screening the enzymes with enhanced substrate and stereo specificities", APPL. MICROBIOL. BIOTECHNOL., vol. 100, no. 6, 26 January 2016 (2016-01-26), pages 2579 - 2590, XP035631555
LEE, S.J. ET AL.: "High-throughput screening methods for selecting 1-threonine aldolases with improved activity", JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC, vol. 26, no. 3-6, 31 December 2003 (2003-12-31), pages 265 - 272, XP002992768
See also references of EP 3630798A4
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