Title:
ENGINEERING BACTERIA FOR PRODUCING β-ELEMENE, CONSTRUCTION METHOD THEREFOR, AND APPLICATION
Document Type and Number:
WIPO Patent Application WO/2022/222213
Kind Code:
A1
Abstract:
Provided is recombinant bacteria for producing β-elemene. Also provided are a method for constructing the recombinant bacteria and a method for producing β-elemene by using the recombinant bacteria. The recombinant bacteria express Solidago canadensis Germacrene A Synthase (ScGAS) and introduce recombinant plasmids containing atoB, idi, and ispA of E. coli and ERG13, tHMG1, ERG12, ERG8, and MVD1 of Saccharomyces cerevisiae. After optimizing same under fermentation conditions, the yield of β-elemene reaches 156.94 mg/L.
Inventors:
YU ZONGXIA (CN)
FENG BAOMIN (CN)
HUO JINYAN (CN)
LU XUAN (CN)
WANG HUIGUO (CN)
CHU XIAOHUI (CN)
WANG XIAOYU (CN)
FENG BAOMIN (CN)
HUO JINYAN (CN)
LU XUAN (CN)
WANG HUIGUO (CN)
CHU XIAOHUI (CN)
WANG XIAOYU (CN)
Application Number:
PCT/CN2021/094795
Publication Date:
October 27, 2022
Filing Date:
May 20, 2021
Export Citation:
Assignee:
UNIV DALIAN (CN)
International Classes:
C12N1/21; C12N9/04; C12N9/10; C12N9/12; C12N9/88; C12N9/90; C12N15/70; C12P5/00; C12R1/19
Foreign References:
| CN112063540A | 2020-12-11 | |||
| CN104120141A | 2014-10-29 | |||
| CN110819650A | 2020-02-21 | |||
| EP3536792A1 | 2019-09-11 |
Other References:
GAO YUNYUN: "The Study of Microbial Synthesis of Germacrene a the Precursor of β-elemene", MASTER THESIS, TIANJIN POLYTECHNIC UNIVERSITY, CN, 15 December 2012 (2012-12-15), CN , XP055979607, ISSN: 1674-0246
ZHU LINFANG: "In Vitro Recombinant Expression and Catalytic Study of the Enzyme Related to the Synthesis of β-elemene by Mevalonate Pathway", MASTER THESIS, TIANJIN POLYTECHNIC UNIVERSITY, CN, 15 January 2019 (2019-01-15), CN , XP055979611, ISSN: 1674-0246
HUO JINYAN, CAO HONGYU; CHU XIAOHUI; FENG BAOMIN; YU ZONGXIA: "Homology modeling and molecular docking of germacrene A synthase", JOURNAL OF TIANJIN NORMAL UNIVERSITY (NATURAL SCIENCE EDITION), vol. 40, no. 6, 30 November 2020 (2020-11-30), pages 24 - 29, XP055979613, ISSN: 1671-1114, DOI: 10.19638/j.issn1671-1114.20200605
JW. D. K. ET AL.: "(+)-Germacrene A biosynthesis - The committed step in the biosynthesis of bitter sesquiterpene lactones in chicory", PLANT PHYSIOLOGY, vol. 17, no. 4, 8 August 1998 (1998-08-08), XP002112686, ISSN: 0032-0889, DOI: 10.1104/pp.117.4.1381
ZHU LINFANG: "In Vitro Recombinant Expression and Catalytic Study of the Enzyme Related to the Synthesis of β-elemene by Mevalonate Pathway", MASTER THESIS, TIANJIN POLYTECHNIC UNIVERSITY, CN, 15 January 2019 (2019-01-15), CN , XP055979611, ISSN: 1674-0246
HUO JINYAN, CAO HONGYU; CHU XIAOHUI; FENG BAOMIN; YU ZONGXIA: "Homology modeling and molecular docking of germacrene A synthase", JOURNAL OF TIANJIN NORMAL UNIVERSITY (NATURAL SCIENCE EDITION), vol. 40, no. 6, 30 November 2020 (2020-11-30), pages 24 - 29, XP055979613, ISSN: 1671-1114, DOI: 10.19638/j.issn1671-1114.20200605
JW. D. K. ET AL.: "(+)-Germacrene A biosynthesis - The committed step in the biosynthesis of bitter sesquiterpene lactones in chicory", PLANT PHYSIOLOGY, vol. 17, no. 4, 8 August 1998 (1998-08-08), XP002112686, ISSN: 0032-0889, DOI: 10.1104/pp.117.4.1381
Attorney, Agent or Firm:
DALIAN EAST PATENT AGENT LTD. (CN)
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