Title:
ENGINEERING MONOCLONAL ANTIBODIES TO IMPROVE STABILITY AND PRODUCTION TITER
Document Type and Number:
WIPO Patent Application WO/2020/142611
Kind Code:
A3
Abstract:
Presented herein are methods directed to engineering monoclonal antibodies and antibody variants to improve stability and their production in culture. Specifically, the monoclonal antibodies can be engineered at heavy chain residue 56 (AHo numbering) to a glycine, alanine, or serine, and/or engineered at position 80 (AHo) to be a hydrophobic residue such as alanine, isoleucine, phenylalanine, leucine, methionine, or valine.
Inventors:
STEVENS JENNITTE LEANN (US)
TEMEL DENIZ (US)
ESTES BRAM (US)
AGRAWAL NEERAJ JAGDISH (US)
TEMEL DENIZ (US)
ESTES BRAM (US)
AGRAWAL NEERAJ JAGDISH (US)
Application Number:
PCT/US2020/012057
Publication Date:
September 03, 2020
Filing Date:
January 02, 2020
Export Citation:
Assignee:
AMGEN INC (US)
International Classes:
C07K16/00
Domestic Patent References:
| WO2009000099A2 | 2008-12-31 |
Foreign References:
| US20090155255A1 | 2009-06-18 |
Other References:
HONEGGER A ET AL: "Yet Another Numbering Scheme for Immunoglobulin Variable Domains: An Automatic Modeling and Analysis Tool", JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS, UNITED KINGDOM, vol. 309, no. 3, 8 June 2001 (2001-06-08), pages 657 - 670, XP004626893, ISSN: 0022-2836, DOI: 10.1006/JMBI.2001.4662
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