Title:
ENUMERATING MICROORGANISMS USING GELABLE, COLD WATER-SOLUBLE MEDIA
Document Type and Number:
WIPO Patent Application WO/2024/050269
Kind Code:
A3
Abstract:
Provided are methods for enumerating target microorganisms in a test sample, comprising: retaining a liquid test sample having dissolved therein a nutrient base media component and a glucomannan-containing gelling agent component of a solid cold water-soluble microbiological medium; forming a gelled layer of the dissolved medium; incubating the gelled layer under conditions sufficient for microbial growth and detectable colony formation in and/or on the gelled layer; and enumerating a number of target microorganisms in the test sample by counting the colonies. Preferred gelling agents comprises 5-7 g glucomannan per 100 ml liquid test sample, and optionally comprise a gum(s). Compositions, kits and combinations comprising a nutrient base media and a glucomannan-containing gelling agent, are also provided. The compositions and methods enable enumerating a small number of target microorganisms (e.g., < 1 cell/mL) in a large volume (e.g., 50 ml to 150 mL or larger) of a liquid test sample.
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Inventors:
HSU EDDIE FU-CHIH (US)
SAMADPOUR MANSOUR (US)
SAMADPOUR MANSOUR (US)
Application Number:
PCT/US2023/072757
Publication Date:
April 25, 2024
Filing Date:
August 23, 2023
Export Citation:
Assignee:
INST FOR ENVIRONMENTAL HEALTH INC (US)
International Classes:
C12Q1/04; C08B37/00; C12N1/00; C12N1/02
Attorney, Agent or Firm:
DAVISON, Barry L. et al. (US)
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