Title:
ENZYME DETECTION BY MICROFLUIDICS
Document Type and Number:
WIPO Patent Application WO/2013/029631
Kind Code:
A3
Abstract:
Microfluidic-implemented methods of detecting an enzyme, in particular a DNA-modifying enzyme, are provided, as well as methods for detecting a cell, or a microorganism expressing said enzyme. The enzyme is detected by providing a nucleic acid substrate, which is specifically targeted by that enzyme.
Inventors:
KOCH JOERN ERLAND (DK)
STOUGAARD MAGNUS (DK)
ANDERSEN BIRGITTA RUTH (DK)
JUUL SISSEL (DK)
LEONG KAM (US)
HO MEGAN YI-PING (US)
ANDERSEN FELICIE F (DK)
STOUGAARD MAGNUS (DK)
ANDERSEN BIRGITTA RUTH (DK)
JUUL SISSEL (DK)
LEONG KAM (US)
HO MEGAN YI-PING (US)
ANDERSEN FELICIE F (DK)
Application Number:
PCT/DK2012/050327
Publication Date:
May 10, 2013
Filing Date:
August 31, 2012
Export Citation:
Assignee:
KOCH JOERN ERLAND (DK)
STOUGAARD MAGNUS (DK)
ANDERSEN BIRGITTA RUTH (DK)
JUUL SISSEL (DK)
LEONG KAM (US)
HO MEGAN YI-PING (US)
ANDERSEN FELICIE F (DK)
STOUGAARD MAGNUS (DK)
ANDERSEN BIRGITTA RUTH (DK)
JUUL SISSEL (DK)
LEONG KAM (US)
HO MEGAN YI-PING (US)
ANDERSEN FELICIE F (DK)
International Classes:
C12Q1/00
Other References:
SISSEL JUUL ET AL: "Microfluidics-mediated isothermal detection of enzyme activity at the single molecule level", ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY,EMBC, 2011 ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE, IEEE, 30 August 2011 (2011-08-30), pages 3258 - 3261, XP032025888, ISBN: 978-1-4244-4121-1, DOI: 10.1109/IEMBS.2011.6090885
WEILIN XU ET AL: "Exploring both sequence detection and restriction endonuclease cleavage kinetics by recognition site via single-molecule microfluidic trapping", LAB ON A CHIP ROYAL SOCIETY OF CHEMISTRY UK, vol. 11, no. 3, 7 February 2011 (2011-02-07), pages 435 - 442, XP002693006, ISSN: 1473-0197
JUUL S ET AL: "Detection of single enzymatic events in rare or single cells using microfluidics", 25 October 2011, ACS NANO 20111025 AMERICAN CHEMICAL SOCIETY USA, VOL. 5, NR. 10, PAGE(S) 8305 - 8310, XP002693007
KOVARIK MICHELLE L ET AL: "Micro total analysis systems: fundamental advances and applications in the laboratory, clinic, and field.", 15 January 2013, ANALYTICAL CHEMISTRY 15 JAN 2013, VOL. 85, NR. 2, PAGE(S) 451 - 472, ISSN: 1520-6882, XP002693008
See also references of EP 2751277A2
WEILIN XU ET AL: "Exploring both sequence detection and restriction endonuclease cleavage kinetics by recognition site via single-molecule microfluidic trapping", LAB ON A CHIP ROYAL SOCIETY OF CHEMISTRY UK, vol. 11, no. 3, 7 February 2011 (2011-02-07), pages 435 - 442, XP002693006, ISSN: 1473-0197
JUUL S ET AL: "Detection of single enzymatic events in rare or single cells using microfluidics", 25 October 2011, ACS NANO 20111025 AMERICAN CHEMICAL SOCIETY USA, VOL. 5, NR. 10, PAGE(S) 8305 - 8310, XP002693007
KOVARIK MICHELLE L ET AL: "Micro total analysis systems: fundamental advances and applications in the laboratory, clinic, and field.", 15 January 2013, ANALYTICAL CHEMISTRY 15 JAN 2013, VOL. 85, NR. 2, PAGE(S) 451 - 472, ISSN: 1520-6882, XP002693008
See also references of EP 2751277A2
Attorney, Agent or Firm:
HØIBERG A/S (Copenhagen K, DK)
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