Title:
ENZYME IMMOBILIZATION CARRIER AND PREPARATION METHOD THEREFOR, IMMOBILIZED ENZYME AND PREPARATION METHOD THEREFOR, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/066402
Kind Code:
A1
Abstract:
Provided are an enzyme immobilization carrier and a preparation method therefor, an immobilized enzyme and a preparation method therefor, and use thereof. The enzyme immobilization carrier comprises a resin matrix and a divinyl sulfone group modified on the resin matrix by means of a covalent bond. In order to realize the directed immobilization of an enzyme, the divinyl sulfone group is loaded on the resin matrix by means of the covalent bond. In a subsequent enzyme immobilization reaction, by using specific binding of the divinyl sulfone group to a histidine tag, the divinyl sulfone group in the enzyme immobilization carrier is connected to a specific amino acid residue in an enzyme molecule by means of a covalent bond, without reacting with an active group in the enzyme molecule, thereby effectively avoiding excessive influence of the enzyme immobilization carrier on enzyme conformation, and thus retaining a high catalytic activity of the enzyme. Compared with a traditional metal chelating type resin-enzyme molecule binding mode, the present invention shows better immobility.
Inventors:
HONG HAO (US)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
PAN LONG (CN)
MA LITENG (CN)
CUI YUXIA (CN)
HOU BINGYANG (CN)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
PAN LONG (CN)
MA LITENG (CN)
CUI YUXIA (CN)
HOU BINGYANG (CN)
Application Number:
PCT/CN2023/095332
Publication Date:
April 04, 2024
Filing Date:
May 19, 2023
Export Citation:
Assignee:
ASYMCHEM LABORATORIES FUXIN CO LTD (CN)
International Classes:
C12N11/08; C12N9/04; C12N9/10; C12P7/22; C12P17/12; C12P19/40
Domestic Patent References:
| WO1993001498A1 | 1993-01-21 |
Foreign References:
| CN101848988A | 2010-09-29 | |||
| CN110078829A | 2019-08-02 | |||
| CN108503565A | 2018-09-07 | |||
| JP2003194815A | 2003-07-09 | |||
| CN115537410A | 2022-12-30 |
Other References:
MORELLON-STERLING ROBERTO, CARBALLARES DIEGO, ARANA-PEÑA SARA, SIAR EL-HOCINE, BRAHAM SABRINA AIT, FERNANDEZ-LAFUENTE ROBERTO: "Advantages of Supports Activated with Divinyl Sulfone in Enzyme Coimmobilization: Possibility of Multipoint Covalent Immobilization of the Most Stable Enzyme and Immobilization via Ion Exchange of the Least Stable Enzyme", ACS SUSTAINABLE CHEMISTRY & ENGINEERING, AMERICAN CHEMICAL SOCIETY, US, vol. 9, no. 22, 7 June 2021 (2021-06-07), US , pages 7508 - 7518, XP093154259, ISSN: 2168-0485, DOI: 10.1021/acssuschemeng.1c01065
LI MINGYANG, CHENG FANG, LI HAOQIANG, JIN WEIWEI, CHEN CHEN, HE WEI, CHENG GANG, WANG QING: "Site-Specific and Covalent Immobilization of His-Tagged Proteins via Surface Vinyl Sulfone–Imidazole Coupling", LANGMUIR, AMERICAN CHEMICAL SOCIETY, US, vol. 35, no. 50, 17 December 2019 (2019-12-17), US , pages 16466 - 16475, XP055832702, ISSN: 0743-7463, DOI: 10.1021/acs.langmuir.9b02933
JULIA MORALES-SANFRUTOS, LOPEZ-JARAMILLO JAVIER, ORTEGA-MUÑOZ MARIANO, MEGIA-FERNANDEZ ALICIA, PEREZ-BALDERAS FRANCISCO, HERNANDEZ: "Vinyl sulfone: a versatile function for simple bioconjugation and immobilization", ORGANIC & BIOMOLECULAR CHEMISTRY, ROYAL SOCIETY OF CHEMISTRY, vol. 8, no. 3, 1 January 2010 (2010-01-01), pages 667, XP055018208, ISSN: 14770520, DOI: 10.1039/B920576D
LI MINGYANG, CHENG FANG, LI HAOQIANG, JIN WEIWEI, CHEN CHEN, HE WEI, CHENG GANG, WANG QING: "Site-Specific and Covalent Immobilization of His-Tagged Proteins via Surface Vinyl Sulfone–Imidazole Coupling", LANGMUIR, AMERICAN CHEMICAL SOCIETY, US, vol. 35, no. 50, 17 December 2019 (2019-12-17), US , pages 16466 - 16475, XP055832702, ISSN: 0743-7463, DOI: 10.1021/acs.langmuir.9b02933
JULIA MORALES-SANFRUTOS, LOPEZ-JARAMILLO JAVIER, ORTEGA-MUÑOZ MARIANO, MEGIA-FERNANDEZ ALICIA, PEREZ-BALDERAS FRANCISCO, HERNANDEZ: "Vinyl sulfone: a versatile function for simple bioconjugation and immobilization", ORGANIC & BIOMOLECULAR CHEMISTRY, ROYAL SOCIETY OF CHEMISTRY, vol. 8, no. 3, 1 January 2010 (2010-01-01), pages 667, XP055018208, ISSN: 14770520, DOI: 10.1039/B920576D
Attorney, Agent or Firm:
KANGXIN PARTNERS , P.C. (CN)
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